FI57782C - Foerfarande och reagens foer enzymkinetisk koncentrationsbestaemning hos ett substrat - Google Patents
Foerfarande och reagens foer enzymkinetisk koncentrationsbestaemning hos ett substrat Download PDFInfo
- Publication number
- FI57782C FI57782C FI2876/74A FI287674A FI57782C FI 57782 C FI57782 C FI 57782C FI 2876/74 A FI2876/74 A FI 2876/74A FI 287674 A FI287674 A FI 287674A FI 57782 C FI57782 C FI 57782C
- Authority
- FI
- Finland
- Prior art keywords
- substrate
- reaction
- enzyme
- concentration
- inhibitor
- Prior art date
Links
- 230000002255 enzymatic effect Effects 0.000 title claims description 13
- 239000000758 substrate Substances 0.000 claims description 77
- 238000000034 method Methods 0.000 claims description 70
- 238000006243 chemical reaction Methods 0.000 claims description 60
- 102000004190 Enzymes Human genes 0.000 claims description 59
- 108090000790 Enzymes Proteins 0.000 claims description 59
- 239000003112 inhibitor Substances 0.000 claims description 55
- 238000005259 measurement Methods 0.000 claims description 33
- 239000003153 chemical reaction reagent Substances 0.000 claims description 20
- 239000000872 buffer Substances 0.000 claims description 17
- 238000012360 testing method Methods 0.000 claims description 17
- 230000005764 inhibitory process Effects 0.000 claims description 15
- 239000000203 mixture Substances 0.000 claims description 15
- 230000035945 sensitivity Effects 0.000 claims description 15
- 210000002966 serum Anatomy 0.000 claims description 14
- 230000002401 inhibitory effect Effects 0.000 claims description 11
- 210000002381 plasma Anatomy 0.000 claims description 11
- 238000004458 analytical method Methods 0.000 claims description 7
- 230000002860 competitive effect Effects 0.000 claims description 7
- 230000008569 process Effects 0.000 claims description 6
- 238000006911 enzymatic reaction Methods 0.000 claims description 5
- 239000000126 substance Substances 0.000 claims description 4
- 230000008859 change Effects 0.000 claims description 3
- 239000007795 chemical reaction product Substances 0.000 claims description 3
- 238000009499 grossing Methods 0.000 claims description 2
- 239000003381 stabilizer Substances 0.000 claims description 2
- 239000007858 starting material Substances 0.000 claims description 2
- 229940088598 enzyme Drugs 0.000 description 52
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 28
- 229960001031 glucose Drugs 0.000 description 27
- 239000008103 glucose Substances 0.000 description 27
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 26
- ZNNZYHKDIALBAK-UHFFFAOYSA-M potassium thiocyanate Chemical compound [K+].[S-]C#N ZNNZYHKDIALBAK-UHFFFAOYSA-M 0.000 description 23
- 238000003556 assay Methods 0.000 description 20
- LEHOTFFKMJEONL-UHFFFAOYSA-N Uric Acid Chemical compound N1C(=O)NC(=O)C2=C1NC(=O)N2 LEHOTFFKMJEONL-UHFFFAOYSA-N 0.000 description 18
- TVWHNULVHGKJHS-UHFFFAOYSA-N Uric acid Natural products N1C(=O)NC(=O)C2NC(=O)NC21 TVWHNULVHGKJHS-UHFFFAOYSA-N 0.000 description 18
- 229940116269 uric acid Drugs 0.000 description 18
- 239000000523 sample Substances 0.000 description 17
- 230000000694 effects Effects 0.000 description 13
- 108010092464 Urate Oxidase Proteins 0.000 description 11
- 239000000243 solution Substances 0.000 description 11
- VGEREEWJJVICBM-UHFFFAOYSA-N phloretin Chemical compound C1=CC(O)=CC=C1CCC(=O)C1=C(O)C=C(O)C=C1O VGEREEWJJVICBM-UHFFFAOYSA-N 0.000 description 10
- 229940116357 potassium thiocyanate Drugs 0.000 description 10
- LRFVTYWOQMYALW-UHFFFAOYSA-N 9H-xanthine Chemical compound O=C1NC(=O)NC2=C1NC=N2 LRFVTYWOQMYALW-UHFFFAOYSA-N 0.000 description 8
- 108010050375 Glucose 1-Dehydrogenase Proteins 0.000 description 8
- 230000008033 biological extinction Effects 0.000 description 8
- 238000010790 dilution Methods 0.000 description 7
- 239000012895 dilution Substances 0.000 description 7
- 238000004364 calculation method Methods 0.000 description 6
- ZWTDXYUDJYDHJR-UHFFFAOYSA-N (E)-1-(2,4-dihydroxyphenyl)-3-(2,4-dihydroxyphenyl)-2-propen-1-one Natural products OC1=CC(O)=CC=C1C=CC(=O)C1=CC=C(O)C=C1O ZWTDXYUDJYDHJR-UHFFFAOYSA-N 0.000 description 5
- 102000005548 Hexokinase Human genes 0.000 description 5
- 108700040460 Hexokinases Proteins 0.000 description 5
- BAWFJGJZGIEFAR-NNYOXOHSSA-N NAD zwitterion Chemical compound NC(=O)C1=CC=C[N+]([C@H]2[C@@H]([C@H](O)[C@@H](COP([O-])(=O)OP(O)(=O)OC[C@@H]3[C@H]([C@@H](O)[C@@H](O3)N3C4=NC=NC(N)=C4N=C3)O)O2)O)=C1 BAWFJGJZGIEFAR-NNYOXOHSSA-N 0.000 description 5
- YQHMWTPYORBCMF-UHFFFAOYSA-N Naringenin chalcone Natural products C1=CC(O)=CC=C1C=CC(=O)C1=C(O)C=C(O)C=C1O YQHMWTPYORBCMF-UHFFFAOYSA-N 0.000 description 5
- 229950006238 nadide Drugs 0.000 description 5
- 239000012085 test solution Substances 0.000 description 5
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 description 4
- 230000008901 benefit Effects 0.000 description 4
- 238000006555 catalytic reaction Methods 0.000 description 4
- 150000001875 compounds Chemical class 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 4
- 238000003367 kinetic assay Methods 0.000 description 4
- 229930027945 nicotinamide-adenine dinucleotide Natural products 0.000 description 4
- 102000004169 proteins and genes Human genes 0.000 description 4
- 108090000623 proteins and genes Proteins 0.000 description 4
- 239000012086 standard solution Substances 0.000 description 4
- 229940075420 xanthine Drugs 0.000 description 4
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 3
- 108010015776 Glucose oxidase Proteins 0.000 description 3
- 239000004366 Glucose oxidase Substances 0.000 description 3
- ZMZDMBWJUHKJPS-UHFFFAOYSA-M Thiocyanate anion Chemical compound [S-]C#N ZMZDMBWJUHKJPS-UHFFFAOYSA-M 0.000 description 3
- 238000008114 Uric Acid Assay Methods 0.000 description 3
- 102000020006 aldose 1-epimerase Human genes 0.000 description 3
- 108091022872 aldose 1-epimerase Proteins 0.000 description 3
- 238000001514 detection method Methods 0.000 description 3
- 229940116332 glucose oxidase Drugs 0.000 description 3
- 235000019420 glucose oxidase Nutrition 0.000 description 3
- ZMZDMBWJUHKJPS-UHFFFAOYSA-N hydrogen thiocyanate Natural products SC#N ZMZDMBWJUHKJPS-UHFFFAOYSA-N 0.000 description 3
- BOPGDPNILDQYTO-NNYOXOHSSA-N nicotinamide-adenine dinucleotide Chemical compound C1=CCC(C(=O)N)=CN1[C@H]1[C@H](O)[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OC[C@@H]2[C@H]([C@@H](O)[C@@H](O2)N2C3=NC=NC(N)=C3N=C2)O)O1 BOPGDPNILDQYTO-NNYOXOHSSA-N 0.000 description 3
- 239000008363 phosphate buffer Substances 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- WXHLLJAMBQLULT-UHFFFAOYSA-N 2-[[6-[4-(2-hydroxyethyl)piperazin-1-yl]-2-methylpyrimidin-4-yl]amino]-n-(2-methyl-6-sulfanylphenyl)-1,3-thiazole-5-carboxamide;hydrate Chemical compound O.C=1C(N2CCN(CCO)CC2)=NC(C)=NC=1NC(S1)=NC=C1C(=O)NC1=C(C)C=CC=C1S WXHLLJAMBQLULT-UHFFFAOYSA-N 0.000 description 2
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 2
- BTBUEUYNUDRHOZ-UHFFFAOYSA-N Borate Chemical compound [O-]B([O-])[O-] BTBUEUYNUDRHOZ-UHFFFAOYSA-N 0.000 description 2
- QXNVGIXVLWOKEQ-UHFFFAOYSA-N Disodium Chemical class [Na][Na] QXNVGIXVLWOKEQ-UHFFFAOYSA-N 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- POJWUDADGALRAB-UHFFFAOYSA-N allantoin Chemical compound NC(=O)NC1NC(=O)NC1=O POJWUDADGALRAB-UHFFFAOYSA-N 0.000 description 2
- 230000000903 blocking effect Effects 0.000 description 2
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 2
- 238000006073 displacement reaction Methods 0.000 description 2
- 239000012153 distilled water Substances 0.000 description 2
- 229940125532 enzyme inhibitor Drugs 0.000 description 2
- 239000002532 enzyme inhibitor Substances 0.000 description 2
- 230000002452 interceptive effect Effects 0.000 description 2
- XGZVUEUWXADBQD-UHFFFAOYSA-L lithium carbonate Chemical compound [Li+].[Li+].[O-]C([O-])=O XGZVUEUWXADBQD-UHFFFAOYSA-L 0.000 description 2
- 229910052808 lithium carbonate Inorganic materials 0.000 description 2
- 210000004185 liver Anatomy 0.000 description 2
- 239000001103 potassium chloride Substances 0.000 description 2
- 235000011164 potassium chloride Nutrition 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 230000006641 stabilisation Effects 0.000 description 2
- 238000011105 stabilization Methods 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- QCVGEOXPDFCNHA-UHFFFAOYSA-N 5,5-dimethyl-2,4-dioxo-1,3-oxazolidine-3-carboxamide Chemical compound CC1(C)OC(=O)N(C(N)=O)C1=O QCVGEOXPDFCNHA-UHFFFAOYSA-N 0.000 description 1
- BDDLHHRCDSJVKV-UHFFFAOYSA-N 7028-40-2 Chemical compound CC(O)=O.CC(O)=O.CC(O)=O.CC(O)=O BDDLHHRCDSJVKV-UHFFFAOYSA-N 0.000 description 1
- POJWUDADGALRAB-PVQJCKRUSA-N Allantoin Natural products NC(=O)N[C@@H]1NC(=O)NC1=O POJWUDADGALRAB-PVQJCKRUSA-N 0.000 description 1
- 108010089254 Cholesterol oxidase Proteins 0.000 description 1
- 102000002322 Egg Proteins Human genes 0.000 description 1
- 108010000912 Egg Proteins Proteins 0.000 description 1
- 102000057621 Glycerol kinases Human genes 0.000 description 1
- JVTAAEKCZFNVCJ-UHFFFAOYSA-M Lactate Chemical compound CC(O)C([O-])=O JVTAAEKCZFNVCJ-UHFFFAOYSA-M 0.000 description 1
- 108091000080 Phosphotransferase Proteins 0.000 description 1
- 101710163410 Probable glycerol kinase Proteins 0.000 description 1
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 1
- 108010046334 Urease Proteins 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 229960000458 allantoin Drugs 0.000 description 1
- WQZGKKKJIJFFOK-DVKNGEFBSA-N alpha-D-glucose Chemical compound OC[C@H]1O[C@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-DVKNGEFBSA-N 0.000 description 1
- 235000010323 ascorbic acid Nutrition 0.000 description 1
- 229960005070 ascorbic acid Drugs 0.000 description 1
- 239000011668 ascorbic acid Substances 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 239000013060 biological fluid Substances 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 238000004159 blood analysis Methods 0.000 description 1
- 210000000601 blood cell Anatomy 0.000 description 1
- 239000004202 carbamide Substances 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 235000012000 cholesterol Nutrition 0.000 description 1
- 239000000356 contaminant Substances 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 238000007357 dehydrogenase reaction Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 238000011038 discontinuous diafiltration by volume reduction Methods 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229960001484 edetic acid Drugs 0.000 description 1
- 235000014103 egg white Nutrition 0.000 description 1
- 210000000969 egg white Anatomy 0.000 description 1
- 230000008030 elimination Effects 0.000 description 1
- 238000003379 elimination reaction Methods 0.000 description 1
- 238000007824 enzymatic assay Methods 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 230000035876 healing Effects 0.000 description 1
- 238000000265 homogenisation Methods 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 238000012933 kinetic analysis Methods 0.000 description 1
- 238000012417 linear regression Methods 0.000 description 1
- 238000000691 measurement method Methods 0.000 description 1
- 238000011326 mechanical measurement Methods 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 238000003541 multi-stage reaction Methods 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 238000004806 packaging method and process Methods 0.000 description 1
- 230000036961 partial effect Effects 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 102000020233 phosphotransferase Human genes 0.000 description 1
- 238000005375 photometry Methods 0.000 description 1
- 238000005554 pickling Methods 0.000 description 1
- 230000003389 potentiating effect Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000002829 reductive effect Effects 0.000 description 1
- 238000012552 review Methods 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 230000003595 spectral effect Effects 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 230000007480 spreading Effects 0.000 description 1
- 239000011550 stock solution Substances 0.000 description 1
- 238000011282 treatment Methods 0.000 description 1
- UFTFJSFQGQCHQW-UHFFFAOYSA-N triformin Chemical compound O=COCC(OC=O)COC=O UFTFJSFQGQCHQW-UHFFFAOYSA-N 0.000 description 1
- 239000013026 undiluted sample Substances 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
- 238000013389 whole blood assay Methods 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
- C12Q1/32—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving dehydrogenase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/54—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving glucose or galactose
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/62—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving uric acid
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Emergency Medicine (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Claims (16)
1. Förfarande för bestämning av koncentrationen av ett substrat, speciellt ett i blodserum eller blodplasma löst eller suspenderat substrat genom tillsättning av ett eller flera specifika enzymer och bestämning av den enzymatiska reaktionens hastighet, k ä n n e -tecknat av att ätminstone en efter det hastighetsbestämmande steget i den enzymatiska substratomsättningen anpassad konkurrerande inhibitor tillsättes, för justering av förh&llandet av substrat-koncentrationen cg till skenbar Michaelis-konstant K’M tili —0,2:1 och för regiering av reaktionsförhällandena att motsvara en pseudo-första ordningens reaktion,blandningen homogeniseras, för reaktionen specifika reaktionsparametrars förändring bestäms efter bestämda tidsintervall och härav erhälls det undersökta substratets koncen-tration.
2. Förfarande enligt patentkravet l,kännetecknat av att förhällandet av substratkoncentrationen Cg tili skenbar Michaelis-konstant K'^ är högst 0,05:1 och fördelaktigt högst 0,01:1.
3- Förfarande enligt patentkravet 1 eller 2, känneteck- n a t av att s&som konkurrerande inhibitorer används sädana ämnen, som revesibelt binder enzymet och/eller substratet utan att en kemisk reaktion sker. 25 57782 Förfarande enligt patentkravet 3, kännetecknat av att sasom konkurrerande inhibitorer används sädana ämnen, som kemiskt liknar utgängsmaterialets och/eller analysreaktionens slut-produkter.
5· Förfarande enligt nägot av föregäende krav, kännetecknat av att en för analytiska ändamäl lämplig mätkänslighet reg-leras med hjälp av förhällandet av koncentrationerna av substratet, enzymet och den konkurrerande inhibitorn.
6. Förfarande enligt nägot av föregäende krav, kännetecknat av att mätprecisionen ökas genom att höja enzymhalten och/ ^ eller reaktionstemperaturen.
7. Förfarande enligt nägot av föregäende krav, kännetecknat av att bestämningen sker omedelbart efter homogeniseringen, efter en möjligtvis förekommande höjning av temperaturen och/eller en möjlig utjämning av noll-värdet.
8. Förfarande enligt nägot av föregäende krav, kännetecknat av att en möjligen mera tidskrävande utjämning av noll-värdet korrigeras genom att beakta noll-värdet.
9. Förfarande enligt nägot av patentkraven 1-7, kännetecknat av att man använder en buffert som lämpar sig för den sats som skall mätäs och vilken bufferts pH väsentligen motsvarar pH-maximet för denna sats totala reaktionshastighet.
10. Förfarande enligt nägot av föregäende krav, kännetecknat av att man inom pseudo-första ordningens reaktionsomräde i analysserien använder samma tidsintervall efter det att en bestämd vald tiet förlöpt frän reaktionens början (’’time-fixed^-teknik).
11. Förfarande enligt nägot av patentkraven 1-9, känneteck- · n a t av att begynnelsehastigheten bestäms ("initial-rate"-teknik).
12. Förfarande enligt nägot av föregäende krav, kännetecknat av att en lösning av det substrat som skall bestämmas används som redan har en konkurrerande inhiberande verkan. 1 Förfarande enligt patentkravet 12, kännetecknat
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE2349819 | 1973-10-04 | ||
| DE19732349819 DE2349819A1 (de) | 1973-10-04 | 1973-10-04 | Verfahren zur enzymkinetischen konzentrationsbestimmung eines substrats |
| DE19742440011 DE2440011C2 (de) | 1974-08-21 | Verfahren und Reagenz zur enzymkinetischen Konzentrationsbestimmung eines Substrates | |
| DE2440011 | 1974-08-21 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| FI287674A7 FI287674A7 (sv) | 1975-04-05 |
| FI57782B FI57782B (fi) | 1980-06-30 |
| FI57782C true FI57782C (fi) | 1980-10-10 |
Family
ID=25765898
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| FI2876/74A FI57782C (fi) | 1973-10-04 | 1974-10-02 | Foerfarande och reagens foer enzymkinetisk koncentrationsbestaemning hos ett substrat |
Country Status (14)
| Country | Link |
|---|---|
| US (1) | US3977944A (sv) |
| JP (1) | JPS5724119B2 (sv) |
| BE (1) | BE820736A (sv) |
| CA (1) | CA1031676A (sv) |
| CH (1) | CH614534A5 (sv) |
| DD (1) | DD113813A5 (sv) |
| DE (1) | DE2349819A1 (sv) |
| FI (1) | FI57782C (sv) |
| FR (1) | FR2257905B1 (sv) |
| GB (1) | GB1486474A (sv) |
| HU (1) | HU172778B (sv) |
| IT (1) | IT1030641B (sv) |
| NL (1) | NL181936C (sv) |
| SE (1) | SE414409B (sv) |
Families Citing this family (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE2558536B2 (de) * | 1975-12-24 | 1979-07-12 | Boehringer Mannheim Gmbh, 6800 Mannheim | Verfahren zur kinetischen Substratbestimmung und Reagens zu seiner Durchführung |
| US4162194A (en) * | 1976-02-13 | 1979-07-24 | Beckman Instruments, Inc. | Kinetic assay for acid phosphotase and composition therefore |
| US4218535A (en) | 1976-03-16 | 1980-08-19 | Beckman Instruments, Inc. | Determination of enzyme substrate concentration |
| US4159923A (en) * | 1977-01-11 | 1979-07-03 | Beckman Instruments, Inc. | Kinetic assay for inorganic phosphates and composition therefore |
| US4120755A (en) * | 1977-04-28 | 1978-10-17 | Beckman Instruments, Inc. | Kinetic method for determination of glucose concentrations with glucose dehydrogenase |
| FR2418462A1 (fr) * | 1978-02-22 | 1979-09-21 | Anvar | Procede de dosage de substrats intervenant dans des reactions enzymatiques |
| IT1099355B (it) * | 1978-10-12 | 1985-09-18 | Sclavo Inst Sieroterapeut | Composizione adatta alla determinazione in cinetica del glucosio |
| US4322496A (en) | 1980-04-17 | 1982-03-30 | Eastman Kodak Company | Inhibition of lactate oxidase |
| US4318985A (en) * | 1981-01-29 | 1982-03-09 | Miles Laboratories, Inc. | Method and device for detecting glucose concentration |
| IE820943L (en) * | 1982-04-21 | 1983-10-21 | Bartos Patent Dev Holding | Serological investigations by complement fixation test |
| JPS59159796A (ja) * | 1983-03-02 | 1984-09-10 | Oriental Yeast Co Ltd | 酵素活性の測定方法 |
| DE3340709A1 (de) * | 1983-11-10 | 1985-05-23 | Boehringer Mannheim Gmbh, 6800 Mannheim | Kompetitiver inhibitor fuer gk |
| EP0309525A1 (en) * | 1987-04-10 | 1989-04-05 | The Flinders University Of South Australia | Determination of ions in fluids |
| FI81120C (sv) * | 1988-09-26 | 1990-09-10 | Kone Oy | Förfarande för bestämning av glukos ur biologiska vätska samt reagensb landning för tillämpning av förfarandet |
| SE466158B (sv) * | 1989-04-25 | 1992-01-07 | Migrata Uk Ltd | Analysmetod foer glukosbestaemning i helblod |
| US5429930A (en) * | 1989-07-21 | 1995-07-04 | Bio-Research Products, Inc. | Kinetic enzyme assay for determining the CO2 content of body fluids using PEP carboxylase with inhibitor |
| US20030068655A1 (en) * | 2001-09-12 | 2003-04-10 | Protiveris, Inc. | Microcantilever apparatus and methods for detection of enzymes |
| SE523240C2 (sv) * | 2001-12-12 | 2004-04-06 | Akzo Nobel Nv | Användning av hydroxyetylsubstituerad amin som korrosionsinhibitor i saltvattenhaltig miljö i oljefältsapplikationer |
| US7555933B2 (en) * | 2006-08-01 | 2009-07-07 | Thermo Fisher Scientific Inc. | Method and software for detecting vacuum concentrator ends-of-runs |
| DE102016121553A1 (de) * | 2016-11-10 | 2018-05-17 | Technische Universität Darmstadt | Kompetitiver Test eines Enzymsubstrates mit interner Kompensation der Enzymaktivität |
| CN114577745A (zh) * | 2022-02-28 | 2022-06-03 | 深圳市乐土生物医药有限公司 | 一种检测亚甲基四氢叶酸脱氢酶2活性的方法 |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3627645A (en) * | 1969-07-31 | 1971-12-14 | Arequipa Foundation | Method and reagents for the determination of nicotine adenine dinucleotide phosphate and of glutathione |
-
1973
- 1973-10-04 DE DE19732349819 patent/DE2349819A1/de not_active Withdrawn
-
1974
- 1974-10-01 CH CH1319974A patent/CH614534A5/xx not_active IP Right Cessation
- 1974-10-02 FI FI2876/74A patent/FI57782C/fi active
- 1974-10-02 SE SE7412391A patent/SE414409B/sv not_active IP Right Cessation
- 1974-10-02 US US05/511,402 patent/US3977944A/en not_active Expired - Lifetime
- 1974-10-02 IT IT28023/74A patent/IT1030641B/it active
- 1974-10-02 DD DD181464A patent/DD113813A5/xx unknown
- 1974-10-03 GB GB42918/74A patent/GB1486474A/en not_active Expired
- 1974-10-03 NL NLAANVRAGE7413057,A patent/NL181936C/xx not_active IP Right Cessation
- 1974-10-04 FR FR7433550A patent/FR2257905B1/fr not_active Expired
- 1974-10-04 HU HU74EE00002269A patent/HU172778B/hu unknown
- 1974-10-04 JP JP11460774A patent/JPS5724119B2/ja not_active Expired
- 1974-10-04 BE BE149242A patent/BE820736A/xx not_active IP Right Cessation
- 1974-10-04 CA CA210,745A patent/CA1031676A/en not_active Expired
Also Published As
| Publication number | Publication date |
|---|---|
| FI57782B (fi) | 1980-06-30 |
| HU172778B (hu) | 1978-12-28 |
| US3977944A (en) | 1976-08-31 |
| FI287674A7 (sv) | 1975-04-05 |
| NL181936B (nl) | 1987-07-01 |
| DD113813A5 (sv) | 1975-06-20 |
| SE414409B (sv) | 1980-07-28 |
| BE820736A (fr) | 1975-04-04 |
| SE7412391L (sv) | 1975-04-07 |
| NL7413057A (nl) | 1975-04-08 |
| DE2440011A1 (sv) | 1975-10-23 |
| NL181936C (nl) | 1987-12-01 |
| JPS5724119B2 (sv) | 1982-05-22 |
| DE2349819A1 (de) | 1975-04-17 |
| DE2440011B1 (de) | 1975-10-23 |
| JPS5084298A (sv) | 1975-07-08 |
| FR2257905A1 (sv) | 1975-08-08 |
| FR2257905B1 (sv) | 1979-09-28 |
| CA1031676A (en) | 1978-05-23 |
| GB1486474A (en) | 1977-09-21 |
| CH614534A5 (sv) | 1979-11-30 |
| IT1030641B (it) | 1979-04-10 |
| AU7397574A (en) | 1976-04-08 |
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