FR2397637A1 - Procede pour determiner l'activite des isoenzymes - Google Patents

Procede pour determiner l'activite des isoenzymes

Info

Publication number
FR2397637A1
FR2397637A1 FR7820588A FR7820588A FR2397637A1 FR 2397637 A1 FR2397637 A1 FR 2397637A1 FR 7820588 A FR7820588 A FR 7820588A FR 7820588 A FR7820588 A FR 7820588A FR 2397637 A1 FR2397637 A1 FR 2397637A1
Authority
FR
France
Prior art keywords
activity
isoenzyme
isoenzymes
determining
sample
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
FR7820588A
Other languages
English (en)
Other versions
FR2397637B1 (fr
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Eastman Kodak Co
Original Assignee
Eastman Kodak Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Eastman Kodak Co filed Critical Eastman Kodak Co
Publication of FR2397637A1 publication Critical patent/FR2397637A1/fr
Application granted granted Critical
Publication of FR2397637B1 publication Critical patent/FR2397637B1/fr
Granted legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

L'invention est relative à un procédé pour déterminer l'activité des isoenzymes. Pour déterminer l'activité d'une isoenzyme dans un échantillon contenant d'autres isoenzyrnes, on réalise une première mesure de l'activité enzymatique totale dans l'échantillon; puis on ajoute un agent tensio-actif dont la nature et la concentration sont déterminées de façon à obtenir l'inhibition de l'activité d'une isoenzyme spécifique. La différence entre les résultats de la premiere et de la seconde mesures fournit l'activité de cette isoenzyme. Application à la réalisation d'analyses et à l'établissement de diagnostics.
FR7820588A 1977-07-11 1978-07-11 Procede pour determiner l'activite des isoenzymes Granted FR2397637A1 (fr)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US05/814,770 US4250255A (en) 1977-07-11 1977-07-11 Assay method for isoenzyme activity

Publications (2)

Publication Number Publication Date
FR2397637A1 true FR2397637A1 (fr) 1979-02-09
FR2397637B1 FR2397637B1 (fr) 1982-02-19

Family

ID=25215970

Family Applications (1)

Application Number Title Priority Date Filing Date
FR7820588A Granted FR2397637A1 (fr) 1977-07-11 1978-07-11 Procede pour determiner l'activite des isoenzymes

Country Status (6)

Country Link
US (1) US4250255A (fr)
JP (1) JPS5434298A (fr)
CA (1) CA1116061A (fr)
DE (1) DE2830453C2 (fr)
FR (1) FR2397637A1 (fr)
GB (1) GB2000869B (fr)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS60141299A (ja) * 1983-12-28 1985-07-26 Wako Pure Chem Ind Ltd 脱水素酵素の活性度測定法
JPS60196569A (ja) * 1984-03-19 1985-10-05 三洋電機株式会社 冷媒流量制御装置
JPS60196570A (ja) * 1984-03-19 1985-10-05 三洋電機株式会社 冷媒流量制御装置
US4803159A (en) * 1985-06-03 1989-02-07 Eastman Kodak Company Analytical element and method for determination of total lacate dehydrogenase
US5364765A (en) * 1987-05-28 1994-11-15 Abbott William A Method and reagent system for assaying isoenzyme profiles
US5158873A (en) * 1987-05-28 1992-10-27 Abbott Laboratories Method and reagent for determining LD-1 isoenzyme
ES2036234T3 (es) * 1987-05-28 1993-05-16 Abbott Laboratories Un metodo para determinar la actividad de la isoenzima dl-1 de fluidos biologicos.
GB8816443D0 (en) * 1988-07-11 1988-08-17 Albright & Wilson Liquid enzymatic detergents
US6242208B1 (en) 1988-07-15 2001-06-05 International Reagents Corporation LDH1 assay
EP0401641B1 (fr) * 1989-06-09 1995-01-25 Abbott Laboratories Méthode pour l'essai d'iso-enzymes
IE920779A1 (en) * 1991-03-13 1992-09-23 Du Pont Selective stabilization of lactate dehydrogenase isoenzyme¹ld1 by high molecular weight polyols
EP2640384A1 (fr) 2010-11-18 2013-09-25 Synta Pharmaceuticals Corp. Présélection de patients pour un traitement thérapeutique fondé sur un état hypoxique, avec des agents sensibles à l'oxygène
WO2012068491A1 (fr) 2010-11-18 2012-05-24 Synta Pharmaceuticals Corp. Présélection de sujets pour un traitement thérapeutique par de l'élesclomol sur la base de statut hypoxique
JP2014503499A (ja) 2010-11-18 2014-02-13 シンタ ファーマスーティカルズ コーポレーション 低酸素状態に基づく治療に適した被験体の事前選択
WO2013170182A1 (fr) 2012-05-11 2013-11-14 Synta Pharmaceuticals Corp. Traitement d'un cancer au moyen d'un composé inhibiteur de hsp90

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3326777A (en) * 1964-12-10 1967-06-20 Warner Lambert Pharmaceutical Process for differentiating the isoenzymes of lactic dehydrogenase
US3388044A (en) * 1965-12-07 1968-06-11 Warner Lambert Pharmaceutical Process for differentiating the isoenzymes of lactic dehydrogenase
GB1385319A (en) * 1971-09-22 1975-02-26 Nat Res Dev Enzyme preparations
US3992158A (en) * 1973-08-16 1976-11-16 Eastman Kodak Company Integral analytical element
DE2513407A1 (de) * 1975-03-26 1976-10-14 Schmidt Karlheinz Ein verfahren zur differenzierung der leber- und herzspezifischen lactatdehydrogenase im blutserum
US3994783A (en) * 1975-09-26 1976-11-30 Calbiochem Differential assay of creatine phosphokinase isoenzymes

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
CA1970 *
EXBK/72 *
EXBK/77 *

Also Published As

Publication number Publication date
US4250255A (en) 1981-02-10
FR2397637B1 (fr) 1982-02-19
GB2000869B (en) 1982-01-13
JPS5434298A (en) 1979-03-13
DE2830453C2 (de) 1983-07-07
JPS5728271B2 (fr) 1982-06-15
GB2000869A (en) 1979-01-17
CA1116061A (fr) 1982-01-12
DE2830453A1 (de) 1979-02-01

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