FR2445312A1 - Procede de purification de l'insuline - Google Patents
Procede de purification de l'insulineInfo
- Publication number
- FR2445312A1 FR2445312A1 FR7931346A FR7931346A FR2445312A1 FR 2445312 A1 FR2445312 A1 FR 2445312A1 FR 7931346 A FR7931346 A FR 7931346A FR 7931346 A FR7931346 A FR 7931346A FR 2445312 A1 FR2445312 A1 FR 2445312A1
- Authority
- FR
- France
- Prior art keywords
- insulin
- purified
- purification process
- insulin purification
- eluted
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 title abstract 14
- 102000004877 Insulin Human genes 0.000 title abstract 7
- 108090001061 Insulin Proteins 0.000 title abstract 7
- 229940125396 insulin Drugs 0.000 title abstract 7
- 238000000746 purification Methods 0.000 title 1
- 238000000034 method Methods 0.000 abstract 3
- 229920005654 Sephadex Polymers 0.000 abstract 1
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 abstract 1
- 230000002378 acidificating effect Effects 0.000 abstract 1
- 238000005349 anion exchange Methods 0.000 abstract 1
- 239000012736 aqueous medium Substances 0.000 abstract 1
- 239000004202 carbamide Substances 0.000 abstract 1
- 125000002057 carboxymethyl group Chemical group [H]OC(=O)C([H])([H])[*] 0.000 abstract 1
- 150000001768 cations Chemical class 0.000 abstract 1
- 238000004587 chromatography analysis Methods 0.000 abstract 1
- 229910017053 inorganic salt Inorganic materials 0.000 abstract 1
- 102000004169 proteins and genes Human genes 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
- 125000004964 sulfoalkyl group Chemical group 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/62—Insulins
- C07K14/625—Extraction from natural sources
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- Genetics & Genomics (AREA)
- Gastroenterology & Hepatology (AREA)
- Toxicology (AREA)
- Endocrinology (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Zoology (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Diabetes (AREA)
- Peptides Or Proteins (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
Abstract
L'invention consiste en un procédé de purification de l'insuline en utilisant un échangeur de cations qui est un gel de dextrane réticulé contenant des groupement sulfoalkyle ou carboxyméthyle. L'insuline est éluée, sous forme purifee, avec un milieu aqueux acide contenant de l'urée et une concentration variable d'un sel minéral. L'insuline purifée par le procédé précédent peut avoir été préalablement purifiée par chromatographie sur gel échangeur d'anions. Ce procédé permet d'enlever de l'insuline les protéines autres que l'insuline, sur une échelle industrielle, et avec une perte minimale en insuline.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US97274478A | 1978-12-26 | 1978-12-26 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| FR2445312A1 true FR2445312A1 (fr) | 1980-07-25 |
Family
ID=25520061
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| FR7931346A Withdrawn FR2445312A1 (fr) | 1978-12-26 | 1979-12-20 | Procede de purification de l'insuline |
Country Status (10)
| Country | Link |
|---|---|
| EP (1) | EP0013826A1 (fr) |
| JP (1) | JPS5589252A (fr) |
| AU (1) | AU5397579A (fr) |
| BE (1) | BE880791A (fr) |
| DD (1) | DD148298A5 (fr) |
| DK (1) | DK546879A (fr) |
| FR (1) | FR2445312A1 (fr) |
| GB (1) | GB2038340A (fr) |
| IL (1) | IL58995A0 (fr) |
| PL (1) | PL220804A1 (fr) |
Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DK374579A (da) * | 1979-09-07 | 1981-03-08 | Nordisk Insulinlab | Fremgangsmaade til fremstilling af et injucerbart insulinpraeparat |
| DK366380A (da) * | 1980-08-28 | 1982-03-01 | Novo Industri As | Anvendelsen af grp og salte deraf |
| US4459226A (en) * | 1982-02-26 | 1984-07-10 | Eli Lilly And Company | Process for recovering insulin |
| US4616078A (en) * | 1985-04-08 | 1986-10-07 | Eli Lilly And Company | Process for purifying proinsulin-like materials |
| DE3511269A1 (de) * | 1985-04-12 | 1986-10-09 | Veb Berlin-Chemie, Ddr 1199 Berlin | Verfahren zur reinigung von insulin |
| JP2903593B2 (ja) * | 1989-02-14 | 1999-06-07 | 三菱化学株式会社 | 粒状緩効性窒素肥料の製造方法 |
| KR100341297B1 (ko) * | 1997-03-29 | 2002-11-16 | 주식회사종근당 | 재조합활성형프로인슐린의분리.정제방법 |
| US20040113023A1 (en) | 2001-03-23 | 2004-06-17 | Sebastian Benenowski | Superstructure switching device |
| CN103703015B (zh) | 2011-08-25 | 2019-07-05 | 霍夫曼-拉罗奇有限公司 | 阳离子和阴离子交换层析法 |
| EP2748181B1 (fr) * | 2011-09-30 | 2016-03-30 | GE Healthcare BioProcess R&D AB | Procédé de purification de pro-insuline clivée |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3876623A (en) * | 1973-05-09 | 1975-04-08 | Lilly Co Eli | Process for purifying insulin |
| US3878186A (en) * | 1973-05-09 | 1975-04-15 | Lilly Co Eli | Process for purifying insulin |
| FR2375193A1 (fr) * | 1976-12-27 | 1978-07-21 | Univ Minnesota | Procede de stabilisation, de concentration et de purification de l'insuline |
-
1979
- 1979-12-18 AU AU53975/79A patent/AU5397579A/en not_active Abandoned
- 1979-12-19 IL IL58995A patent/IL58995A0/xx unknown
- 1979-12-20 FR FR7931346A patent/FR2445312A1/fr not_active Withdrawn
- 1979-12-20 DK DK546879A patent/DK546879A/da unknown
- 1979-12-21 GB GB7944028A patent/GB2038340A/en not_active Withdrawn
- 1979-12-21 EP EP79303005A patent/EP0013826A1/fr not_active Withdrawn
- 1979-12-21 BE BE1/96622A patent/BE880791A/fr unknown
- 1979-12-25 JP JP17021879A patent/JPS5589252A/ja active Pending
- 1979-12-27 DD DD79218159A patent/DD148298A5/de unknown
- 1979-12-27 PL PL22080479A patent/PL220804A1/xx unknown
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3876623A (en) * | 1973-05-09 | 1975-04-08 | Lilly Co Eli | Process for purifying insulin |
| US3878186A (en) * | 1973-05-09 | 1975-04-15 | Lilly Co Eli | Process for purifying insulin |
| FR2375193A1 (fr) * | 1976-12-27 | 1978-07-21 | Univ Minnesota | Procede de stabilisation, de concentration et de purification de l'insuline |
Also Published As
| Publication number | Publication date |
|---|---|
| DD148298A5 (de) | 1981-05-20 |
| EP0013826A1 (fr) | 1980-08-06 |
| AU5397579A (en) | 1980-07-03 |
| DK546879A (da) | 1980-06-27 |
| IL58995A0 (en) | 1980-03-31 |
| JPS5589252A (en) | 1980-07-05 |
| BE880791A (fr) | 1980-06-23 |
| GB2038340A (en) | 1980-07-23 |
| PL220804A1 (fr) | 1980-10-06 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| ST | Notification of lapse |