HK64897A - Solubilization and oxidation somatotropin from transformed microorganisms - Google Patents

Solubilization and oxidation somatotropin from transformed microorganisms Download PDF

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Publication number
HK64897A
HK64897A HK64897A HK64897A HK64897A HK 64897 A HK64897 A HK 64897A HK 64897 A HK64897 A HK 64897A HK 64897 A HK64897 A HK 64897A HK 64897 A HK64897 A HK 64897A
Authority
HK
Hong Kong
Prior art keywords
somatotropin
detergent
protein
process according
oxidation
Prior art date
Application number
HK64897A
Other languages
English (en)
French (fr)
Inventor
Timothy W. Evans
Mark W. Knuth
Original Assignee
Pharmacia & Upjohn Company
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
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First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=25011872&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=HK64897(A) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Pharmacia & Upjohn Company filed Critical Pharmacia & Upjohn Company
Publication of HK64897A publication Critical patent/HK64897A/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
    • C07K1/1133—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure by redox-reactions involving cystein/cystin side chains
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/61—Growth hormone [GH], i.e. somatotropin

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Endocrinology (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Toxicology (AREA)
  • Zoology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Claims (8)

  1. Verfahren zum Umwandeln einer unlöslichen Form von Somatotropin oder eines Somatotropinanalogen aus einem transformierten Mikroorganismus in die native Disulfidbindungstransformation, umfassend das Solubilisieren und Oxidieren des Somatotropins, wobei das Solubilisieren in Gegenwart eines Detergens durchgeführt wird, wobei das Detergens Natriumdodecylsulfat oder eine Verbindung der Formel         CH₃-(CH₂)n-CO-NR₁-CHR₂-COOH   (I) bedeutet, worin n eine ganze Zahl von 8 bis 20 bedeutet, R₁ Methyl oder Ethyl bedeutet und R₂ Wasserstoff, Methyl, Ethyl, Isopropyl oder n-Propyl bedeutet, dadurch gekennzeichnet, daß die Oxidation ebenfalls in Gegenwart eines Detergens durchgeführt wird.
  2. Verfahren nach Anspruch 1, worin der Mikroorganismus in einer ungepufferten alkalischen Lösung, vor der Oxidation des Somatotropins lysiert wird.
  3. Verfahren nach Anspruch 1 oder 2, worin das Somatotropin in Gegenwart einer Verbindung der Formel I solubilisiert und oxidiert wird.
  4. Verfahren nach Anspruch 3, worin die Verbindung der Formel I N-Lauroylmethylglycin ist.
  5. Verfahren nach einem der vorhergehenden Ansprüche, worin das Somatotropin oder sein Analoges in einer Konzentration größer als 1 mg/ml oxidiert wird.
  6. Verfahren nach einem der vorhergehenden Ansprüche, worin das Somatotropin oder sein Analoges Rindersomatotropin oder ein Rindersomatotropin-Analoges ist.
  7. Verfahren nach einem der vorhergehenden Ansprüche, worin der Mikroorganismus ein E. Coli-Stamm ist.
  8. Verfahren nach einem der vorhergehenden Ansprüche, worin das Oxidieren das Einführen von Luft in die Mischung von Somatotropin und Detergens umfaßt.
HK64897A 1985-06-26 1997-05-15 Solubilization and oxidation somatotropin from transformed microorganisms HK64897A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US74901685A 1985-06-26 1985-06-26
PCT/US1986/001289 WO1987000204A2 (en) 1985-06-26 1986-06-13 Purification of somatotropin from transformed microorganisms

Publications (1)

Publication Number Publication Date
HK64897A true HK64897A (en) 1997-05-23

Family

ID=25011872

Family Applications (1)

Application Number Title Priority Date Filing Date
HK64897A HK64897A (en) 1985-06-26 1997-05-15 Solubilization and oxidation somatotropin from transformed microorganisms

Country Status (9)

Country Link
US (2) US6916914B1 (de)
EP (2) EP0229110B1 (de)
JP (1) JPH06104070B2 (de)
AT (1) ATE76435T1 (de)
DE (1) DE3685424D1 (de)
ES (1) ES2032383T3 (de)
GR (1) GR3004917T3 (de)
HK (1) HK64897A (de)
WO (1) WO1987000204A2 (de)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE76435T1 (de) * 1985-06-26 1992-06-15 Upjohn Co Solubilisation und oxidation von somatotropin aus transformierten mikroorganismen.
JPH01132598A (ja) * 1987-10-14 1989-05-25 Pitman Moore Inc 変性剤溶液中に含まれる組換え蛋白質における分子内ジスルフィド結合の生成を促進する方法
US4958007A (en) * 1988-05-17 1990-09-18 Schering-Plough Corp. Extraction of human interleukin-4- from bacteria
US5079230A (en) * 1988-09-12 1992-01-07 Pitman-Moore, Inc. Stable bioactive somatotropins
US5023323A (en) * 1990-09-12 1991-06-11 Monsanto Company Method of somatotropin naturation
ZA9711733B (en) 1996-12-31 1998-07-01 Monsanto Co Method for solubilization and naturation of somatotropins
PE20090100A1 (es) * 2007-03-30 2009-02-26 Wyeth Corp Metodos de separacion y deteccion de acetato de bacedoxifeno en composiciones farmaceuticas
CN102089319B (zh) 2008-05-08 2013-11-13 味之素株式会社 蛋白质的重折叠方法
RU2570625C2 (ru) * 2010-03-31 2015-12-10 Амсилк Гмбх Выделение нерастворимых искомых белков

Family Cites Families (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4443539A (en) * 1980-02-05 1984-04-17 The Upjohn Company Process for preparing bovine growth hormone
IL59690A (en) 1980-03-24 1983-11-30 Yeda Res & Dev Production of bovine growth hormone by microorganisms and modified microorganisms adapted to produce it
IE52755B1 (en) 1980-08-26 1988-02-17 Univ California Bovine pre-growth and growth hormone
EP0085036A1 (de) 1982-01-18 1983-08-03 Monsanto Company Verfahren zur Verbesserung der Rindermilchproduktion
EP0103395A3 (de) 1982-08-17 1985-05-22 Biogen N.V. DNS-Sequenzen, rekombinante DNS-Moleküle und Verfahren zur Herstellung von rinderwachstumshormonähnlichen Polypeptiden, mit grosser Ausbeute
US4511502A (en) * 1982-12-22 1985-04-16 Genentech, Inc. Purification and activity assurance of precipitated heterologous proteins
US4512922A (en) * 1982-12-22 1985-04-23 Genentech, Inc. Purification and activity assurance of precipitated heterologous proteins
US4518526A (en) * 1982-12-22 1985-05-21 Genentech, Inc. Purification and activity assurance of precipitated heterologous proteins
US4620948A (en) * 1982-12-22 1986-11-04 Genentech, Inc. Purification and activity assurance of precipitated heterologous proteins
US4511503A (en) * 1982-12-22 1985-04-16 Genentech, Inc. Purification and activity assurance of precipitated heterologous proteins
GR79124B (de) * 1982-12-22 1984-10-02 Genentech Inc
US4599197A (en) * 1982-12-22 1986-07-08 Genentech, Inc. Purification and activity assurance of precipitated heterologous proteins
BG49718A3 (bg) 1983-07-15 1992-01-15 Bio- Technology General Corp Метод за получаване на полипептид със супероксиддисмутазна активност
US4652630A (en) * 1985-02-22 1987-03-24 Monsanto Company Method of somatotropin naturation
US5037806A (en) * 1985-02-22 1991-08-06 Monsanto Company Biologically active method using somatotropins
US4612367A (en) * 1985-04-08 1986-09-16 Eli Lilly And Company Process for purifying growth hormone-like materials
ATE76435T1 (de) * 1985-06-26 1992-06-15 Upjohn Co Solubilisation und oxidation von somatotropin aus transformierten mikroorganismen.
US5023323A (en) * 1990-09-12 1991-06-11 Monsanto Company Method of somatotropin naturation
US5240834A (en) * 1991-01-22 1993-08-31 Albert Einstein College Of Medicine Of Yeshiva University Solubilization of protein after bacterial expression using sarkosyl

Also Published As

Publication number Publication date
ATE76435T1 (de) 1992-06-15
EP0229110A1 (de) 1987-07-22
US6586575B1 (en) 2003-07-01
WO1987000204A2 (en) 1987-01-15
US6916914B1 (en) 2005-07-12
EP0229110B1 (de) 1992-05-20
EP0263902A1 (de) 1988-04-20
ES2032383T3 (es) 1993-02-16
JPH06104070B2 (ja) 1994-12-21
EP0263902B1 (de) 1992-05-27
GR3004917T3 (de) 1993-04-28
JPS62503143A (ja) 1987-12-17
WO1987000204A3 (en) 1987-03-12
DE3685424D1 (de) 1992-06-25

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PC Patent ceased (i.e. patent has lapsed due to the failure to pay the renewal fee)