IE44834L - Determining lipase activity - Google Patents
Determining lipase activityInfo
- Publication number
- IE44834L IE44834L IE90777A IE90777A IE44834L IE 44834 L IE44834 L IE 44834L IE 90777 A IE90777 A IE 90777A IE 90777 A IE90777 A IE 90777A IE 44834 L IE44834 L IE 44834L
- Authority
- IE
- Ireland
- Prior art keywords
- triglyceride
- carrier
- lipase activity
- soluble
- impregnated
- Prior art date
Links
- 235000019626 lipase activity Nutrition 0.000 title abstract 3
- UFTFJSFQGQCHQW-UHFFFAOYSA-N triformin Chemical compound O=COCC(OC=O)COC=O UFTFJSFQGQCHQW-UHFFFAOYSA-N 0.000 abstract 5
- 239000003153 chemical reaction reagent Substances 0.000 abstract 2
- 239000011159 matrix material Substances 0.000 abstract 2
- 239000007787 solid Substances 0.000 abstract 2
- 239000002904 solvent Substances 0.000 abstract 2
- BAECOWNUKCLBPZ-HIUWNOOHSA-N Triolein Natural products O([C@H](OCC(=O)CCCCCCC/C=C\CCCCCCCC)COC(=O)CCCCCCC/C=C\CCCCCCCC)C(=O)CCCCCCC/C=C\CCCCCCCC BAECOWNUKCLBPZ-HIUWNOOHSA-N 0.000 abstract 1
- PHYFQTYBJUILEZ-UHFFFAOYSA-N Trioleoylglycerol Natural products CCCCCCCCC=CCCCCCCCC(=O)OCC(OC(=O)CCCCCCCC=CCCCCCCCC)COC(=O)CCCCCCCC=CCCCCCCCC PHYFQTYBJUILEZ-UHFFFAOYSA-N 0.000 abstract 1
- 239000000839 emulsion Substances 0.000 abstract 1
- 239000012634 fragment Substances 0.000 abstract 1
- PHYFQTYBJUILEZ-IUPFWZBJSA-N triolein Chemical compound CCCCCCCC\C=C/CCCCCCCC(=O)OCC(OC(=O)CCCCCCC\C=C/CCCCCCCC)COC(=O)CCCCCCC\C=C/CCCCCCCC PHYFQTYBJUILEZ-IUPFWZBJSA-N 0.000 abstract 1
- 229940117972 triolein Drugs 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
- C12Q1/44—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving esterase
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The reagent for the determination of lipase activity in a solution consists of a solid matrix of an inert carrier which is soluble in an aqueous reaction solution and is impregnated with a triglyceride. The carrier is preferably impregnated with triolein; the carrier is, for example, a sugar. To prepare the reagent, at least one triglyceride is dissolved in a solvent. The resulting solution is then mixed with an appropriate amount of the inert carrier which is soluble in an aqueous reaction solution. Finally, the solvent is evaporated off so that a solid matrix of the carrier impregnated with the triglyceride remains. The lipase activity in a sample is determined by reacting the sample with an emulsion of a triglyceride in an aqueous reaction solution and measuring the rate at which the triglyceride is hydrolysed to soluble fragments.
[GB1530238A]
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US68297776A | 1976-05-04 | 1976-05-04 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| IE44834L true IE44834L (en) | 1977-11-04 |
| IE44834B1 IE44834B1 (en) | 1982-04-07 |
Family
ID=24742046
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| IE90777A IE44834B1 (en) | 1976-05-04 | 1977-05-04 | Method of determining lipase activity using a novel triglyceride reagent and method for preparing that reagent |
Country Status (12)
| Country | Link |
|---|---|
| JP (1) | JPS52134499A (en) |
| BE (1) | BE854219A (en) |
| CA (1) | CA1109373A (en) |
| CH (1) | CH628929A5 (en) |
| DE (1) | DE2719704A1 (en) |
| DK (1) | DK194577A (en) |
| FR (1) | FR2350604A1 (en) |
| GB (1) | GB1530238A (en) |
| IE (1) | IE44834B1 (en) |
| IT (1) | IT1114613B (en) |
| LU (1) | LU77256A1 (en) |
| NL (1) | NL7704782A (en) |
Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5510447A (en) * | 1978-07-07 | 1980-01-24 | Nippon Electric Co | Oxide permittivity material |
| DE2904305C2 (en) * | 1979-02-05 | 1981-07-02 | Boehringer Mannheim Gmbh, 6800 Mannheim | Lipase determination reagent and process for its preparation |
| DE2905531A1 (en) * | 1979-02-14 | 1981-01-08 | Boehringer Mannheim Gmbh | DIAGNOSTIC AGENT FOR DETECTING LEUCOCYTES IN BODY LIQUIDS |
| EP0021572A1 (en) * | 1979-06-04 | 1981-01-07 | American Hospital Supply Corporation | A substrate for use in a turbidimetric assay for lipase and a method for making this substrate |
| US4555483A (en) * | 1982-08-11 | 1985-11-26 | Eastman Kodak Company | Methods, compositions and elements for the determination of lipase |
| US4820627A (en) * | 1986-03-24 | 1989-04-11 | Em Diagnostic Systems, Inc. | Method of preparing particles suitable for tabletting into diagnostic reagents |
| US5009994A (en) * | 1986-03-24 | 1991-04-23 | Em Diagnostic Systems, Inc. | Particles containing mannitol suitable for tabletting into diagnostic reagents |
| FR2619219A1 (en) * | 1987-08-07 | 1989-02-10 | Strasbourg I Louis Pasteur Uni | Dry reagent for the determination of pancreatic lipase, process for preparing the said dry reagent and dry reagent and reagent in emulsion form obtained by reconstituting the dry reagent |
| JP2711332B2 (en) * | 1988-04-15 | 1998-02-10 | 株式会社ヤトロン | Method for producing freeze-dried product from which transparent aqueous solution of water-insoluble substance is obtained |
| FR2824334B1 (en) | 2001-05-03 | 2003-10-10 | Coletica | METHOD FOR TESTING A SUBSTANCE POSSIBLY ACTIVE IN THE FIELD OF LIPOLYSIS AND ITS MAINLY COSMETIC USE |
| JP5463012B2 (en) * | 2007-05-16 | 2014-04-09 | 富士フイルム株式会社 | Method for producing dry analytical element for pancreatic lipase measurement |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3539450A (en) * | 1966-06-30 | 1970-11-10 | Calbiochem | Stabilization of enzymes |
| ZA707714B (en) * | 1969-12-10 | 1971-09-29 | Boehringer Mannheim Gmbh | Reagent for the determination of lipase activity |
| US3917515A (en) * | 1974-03-13 | 1975-11-04 | Jack M Goldberg | Serum lipase method and medium |
-
1977
- 1977-04-15 GB GB1585977A patent/GB1530238A/en not_active Expired
- 1977-05-02 CA CA277,628A patent/CA1109373A/en not_active Expired
- 1977-05-02 NL NL7704782A patent/NL7704782A/en not_active Application Discontinuation
- 1977-05-03 IT IT2313477A patent/IT1114613B/en active
- 1977-05-03 BE BE177233A patent/BE854219A/en not_active IP Right Cessation
- 1977-05-03 FR FR7713341A patent/FR2350604A1/en active Granted
- 1977-05-03 DK DK194577A patent/DK194577A/en unknown
- 1977-05-03 CH CH554077A patent/CH628929A5/en not_active IP Right Cessation
- 1977-05-03 DE DE19772719704 patent/DE2719704A1/en not_active Withdrawn
- 1977-05-04 IE IE90777A patent/IE44834B1/en unknown
- 1977-05-04 LU LU77256A patent/LU77256A1/xx unknown
- 1977-05-04 JP JP5194277A patent/JPS52134499A/en active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| DE2719704A1 (en) | 1977-11-24 |
| CH628929A5 (en) | 1982-03-31 |
| BE854219A (en) | 1977-11-03 |
| FR2350604A1 (en) | 1977-12-02 |
| LU77256A1 (en) | 1977-12-13 |
| GB1530238A (en) | 1978-10-25 |
| JPS52134499A (en) | 1977-11-10 |
| CA1109373A (en) | 1981-09-22 |
| DK194577A (en) | 1977-11-05 |
| NL7704782A (en) | 1977-11-08 |
| IE44834B1 (en) | 1982-04-07 |
| IT1114613B (en) | 1986-01-27 |
| FR2350604B1 (en) | 1983-08-05 |
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