IE911129A1 - Hepatitis c virus protease - Google Patents

Hepatitis c virus protease

Info

Publication number: IE911129A1
Authority: IE; Ireland
Prior art keywords: gly; thr; leu; val; ala
Prior art date: 1990-04-04

Application number

IE112991A

Other languages

English (en)

Other versions

IE84608B1 (en

Original Assignee

Chiron Corp

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1990-04-04

Filing date

1991-04-04

Publication date

1991-10-09

Publication of IE84608B1 publication Critical patent/IE84608B1/en

1991-04-04 Application filed by Chiron Corp filed Critical Chiron Corp

1991-10-09 Publication of IE911129A1 publication Critical patent/IE911129A1/en

Links

241000711549 Hepacivirus C Species 0.000 title claims abstract description 118
239000004365 Protease Substances 0.000 title claims abstract description 117
108091005804 Peptidases Proteins 0.000 title claims abstract description 114
102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 title claims abstract 16
108090000765 processed proteins & peptides Proteins 0.000 claims abstract description 55
102000004196 processed proteins & peptides Human genes 0.000 claims abstract description 44
229920001184 polypeptide Polymers 0.000 claims abstract description 42
239000013598 vector Substances 0.000 claims description 59
238000000034 method Methods 0.000 claims description 41
108090000623 proteins and genes Proteins 0.000 claims description 41
108091033319 polynucleotide Proteins 0.000 claims description 32
102000040430 polynucleotide Human genes 0.000 claims description 32
239000002157 polynucleotide Substances 0.000 claims description 32
230000004927 fusion Effects 0.000 claims description 27
239000000203 mixture Substances 0.000 claims description 26
108020001507 fusion proteins Proteins 0.000 claims description 25
230000000694 effects Effects 0.000 claims description 24
102000037865 fusion proteins Human genes 0.000 claims description 24
240000004808 Saccharomyces cerevisiae Species 0.000 claims description 21
102000004169 proteins and genes Human genes 0.000 claims description 21
239000013604 expression vector Substances 0.000 claims description 19
YOKVEHGYYQEQOP-QWRGUYRKSA-N Leu-Leu-Gly Chemical compound CC(C)C[C@H](N)C(=O)N[C@@H](CC(C)C)C(=O)NCC(O)=O YOKVEHGYYQEQOP-QWRGUYRKSA-N 0.000 claims description 16
TWLMXDWFVNEFFK-FJXKBIBVSA-N Thr-Arg-Gly Chemical compound [H]N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)NCC(O)=O TWLMXDWFVNEFFK-FJXKBIBVSA-N 0.000 claims description 14
IASNWHAGGYTEKX-IUCAKERBSA-N Arg-Arg-Gly Chemical compound NC(N)=NCCC[C@H](N)C(=O)N[C@@H](CCCN=C(N)N)C(=O)NCC(O)=O IASNWHAGGYTEKX-IUCAKERBSA-N 0.000 claims description 13
108090000848 Ubiquitin Proteins 0.000 claims description 13
102000044159 Ubiquitin Human genes 0.000 claims description 12
YXTFLTJYLIAZQG-FJXKBIBVSA-N Gly-Thr-Arg Chemical compound NCC(=O)N[C@@H]([C@H](O)C)C(=O)N[C@H](C(O)=O)CCCN=C(N)N YXTFLTJYLIAZQG-FJXKBIBVSA-N 0.000 claims description 10
125000002924 primary amino group Chemical group [H]N([H])* 0.000 claims description 10
KZNQNBZMBZJQJO-UHFFFAOYSA-N N-glycyl-L-proline Natural products NCC(=O)N1CCCC1C(O)=O KZNQNBZMBZJQJO-UHFFFAOYSA-N 0.000 claims description 9
KCRQEJSKXAIULJ-FJXKBIBVSA-N Thr-Gly-Arg Chemical compound [H]N[C@@H]([C@@H](C)O)C(=O)NCC(=O)N[C@@H](CCCNC(N)=N)C(O)=O KCRQEJSKXAIULJ-FJXKBIBVSA-N 0.000 claims description 9
AHERARIZBPOMNU-KATARQTJSA-N Thr-Ser-Leu Chemical compound [H]N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CO)C(=O)N[C@@H](CC(C)C)C(O)=O AHERARIZBPOMNU-KATARQTJSA-N 0.000 claims description 9
108010050848 glycylleucine Proteins 0.000 claims description 9
SITLTJHOQZFJGG-UHFFFAOYSA-N N-L-alpha-glutamyl-L-valine Natural products CC(C)C(C(O)=O)NC(=O)C(N)CCC(O)=O SITLTJHOQZFJGG-UHFFFAOYSA-N 0.000 claims description 8
WNQJTLATMXYSEL-OEAJRASXSA-N Thr-Phe-Leu Chemical compound [H]N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CC1=CC=CC=C1)C(=O)N[C@@H](CC(C)C)C(O)=O WNQJTLATMXYSEL-OEAJRASXSA-N 0.000 claims description 8
150000001875 compounds Chemical class 0.000 claims description 8
230000036961 partial effect Effects 0.000 claims description 8
108010002311 N-glycylglutamic acid Proteins 0.000 claims description 7
WWXNZNWZNZPDIF-SRVKXCTJSA-N Pro-Val-Arg Chemical compound NC(N)=NCCC[C@@H](C(O)=O)NC(=O)[C@H](C(C)C)NC(=O)[C@@H]1CCCN1 WWXNZNWZNZPDIF-SRVKXCTJSA-N 0.000 claims description 7
IMNVAOPEMFDAQD-NHCYSSNCSA-N Pro-Val-Glu Chemical compound [H]N1CCC[C@H]1C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CCC(O)=O)C(O)=O IMNVAOPEMFDAQD-NHCYSSNCSA-N 0.000 claims description 7
DGDCHPCRMWEOJR-FQPOAREZSA-N Thr-Ala-Tyr Chemical compound C[C@@H](O)[C@H](N)C(=O)N[C@@H](C)C(=O)N[C@H](C(O)=O)CC1=CC=C(O)C=C1 DGDCHPCRMWEOJR-FQPOAREZSA-N 0.000 claims description 7
VVZDBPBZHLQPPB-XVKPBYJWSA-N Val-Glu-Gly Chemical compound CC(C)[C@H](N)C(=O)N[C@@H](CCC(O)=O)C(=O)NCC(O)=O VVZDBPBZHLQPPB-XVKPBYJWSA-N 0.000 claims description 7
UJMCYJKPDFQLHX-XGEHTFHBSA-N Val-Ser-Thr Chemical compound C[C@H]([C@@H](C(=O)O)NC(=O)[C@H](CO)NC(=O)[C@H](C(C)C)N)O UJMCYJKPDFQLHX-XGEHTFHBSA-N 0.000 claims description 7
108010045350 alanyl-tyrosyl-alanine Proteins 0.000 claims description 7
108010013835 arginine glutamate Proteins 0.000 claims description 7
108010051307 glycyl-glycyl-proline Proteins 0.000 claims description 7
108010037850 glycylvaline Proteins 0.000 claims description 7
230000002797 proteolythic effect Effects 0.000 claims description 7
108010001336 Horseradish Peroxidase Proteins 0.000 claims description 6
MUJQWSAWLLRJCE-KATARQTJSA-N Ser-Leu-Thr Chemical compound [H]N[C@@H](CO)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H]([C@@H](C)O)C(O)=O MUJQWSAWLLRJCE-KATARQTJSA-N 0.000 claims description 6
FJWSJWACLMTDMI-WPRPVWTQSA-N Gly-Met-Val Chemical compound [H]NCC(=O)N[C@@H](CCSC)C(=O)N[C@@H](C(C)C)C(O)=O FJWSJWACLMTDMI-WPRPVWTQSA-N 0.000 claims description 5
JHVNNUIQXOGAHI-KJEVXHAQSA-N Met-Tyr-Thr Chemical compound C[C@H]([C@@H](C(=O)O)NC(=O)[C@H](CC1=CC=C(C=C1)O)NC(=O)[C@H](CCSC)N)O JHVNNUIQXOGAHI-KJEVXHAQSA-N 0.000 claims description 5
AJHCSUXXECOXOY-UHFFFAOYSA-N N-glycyl-L-tryptophan Natural products C1=CC=C2C(CC(NC(=O)CN)C(O)=O)=CNC2=C1 AJHCSUXXECOXOY-UHFFFAOYSA-N 0.000 claims description 5
PLQWGQUNUPMNOD-KKUMJFAQSA-N Ser-Tyr-Leu Chemical compound [H]N[C@@H](CO)C(=O)N[C@@H](CC1=CC=C(O)C=C1)C(=O)N[C@@H](CC(C)C)C(O)=O PLQWGQUNUPMNOD-KKUMJFAQSA-N 0.000 claims description 5
108010022999 Serine Proteases Proteins 0.000 claims description 5
102000012479 Serine Proteases Human genes 0.000 claims description 5
OBVSBEYOMDWLRJ-BFHQHQDPSA-N Ala-Gly-Thr Chemical compound C[C@@H](O)[C@@H](C(O)=O)NC(=O)CNC(=O)[C@H](C)N OBVSBEYOMDWLRJ-BFHQHQDPSA-N 0.000 claims description 4
NZGRHTKZFSVPAN-BIIVOSGPSA-N Ala-Ser-Pro Chemical compound C[C@@H](C(=O)N[C@@H](CO)C(=O)N1CCC[C@@H]1C(=O)O)N NZGRHTKZFSVPAN-BIIVOSGPSA-N 0.000 claims description 4
KARBMKZDLYMMOW-JYBASQMISA-N Cys-Trp-Thr Chemical compound C[C@H]([C@@H](C(=O)O)NC(=O)[C@H](CC1=CNC2=CC=CC=C21)NC(=O)[C@H](CS)N)O KARBMKZDLYMMOW-JYBASQMISA-N 0.000 claims description 4
239000004366 Glucose oxidase Substances 0.000 claims description 4
108010015776 Glucose oxidase Proteins 0.000 claims description 4
BMWFDYIYBAFROD-WPRPVWTQSA-N Gly-Pro-Val Chemical compound CC(C)[C@@H](C(O)=O)NC(=O)[C@@H]1CCCN1C(=O)CN BMWFDYIYBAFROD-WPRPVWTQSA-N 0.000 claims description 4
CDKZJGMPZHPAJC-ULQDDVLXSA-N Tyr-Leu-Val Chemical compound CC(C)[C@@H](C(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@@H](N)CC1=CC=C(O)C=C1 CDKZJGMPZHPAJC-ULQDDVLXSA-N 0.000 claims description 4
108010069926 arginyl-glycyl-serine Proteins 0.000 claims description 4
229940116332 glucose oxidase Drugs 0.000 claims description 4
235000019420 glucose oxidase Nutrition 0.000 claims description 4
OOWSBIOUKIUWLO-RCOVLWMOSA-N Asn-Gly-Val Chemical compound [H]N[C@@H](CC(N)=O)C(=O)NCC(=O)N[C@@H](C(C)C)C(O)=O OOWSBIOUKIUWLO-RCOVLWMOSA-N 0.000 claims description 3
XWSIYTYNLKCLJB-CIUDSAMLSA-N Asp-Lys-Asn Chemical compound [H]N[C@@H](CC(O)=O)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(N)=O)C(O)=O XWSIYTYNLKCLJB-CIUDSAMLSA-N 0.000 claims description 3
XTHUKRLJRUVVBF-WHFBIAKZSA-N Cys-Gly-Ser Chemical compound SC[C@H](N)C(=O)NCC(=O)N[C@@H](CO)C(O)=O XTHUKRLJRUVVBF-WHFBIAKZSA-N 0.000 claims description 3
LHSGPCFBGJHPCY-UHFFFAOYSA-N L-leucine-L-tyrosine Natural products CC(C)CC(N)C(=O)NC(C(O)=O)CC1=CC=C(O)C=C1 LHSGPCFBGJHPCY-UHFFFAOYSA-N 0.000 claims description 3
RXGLHDWAZQECBI-SRVKXCTJSA-N Leu-Leu-Ser Chemical compound CC(C)C[C@H](N)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CO)C(O)=O RXGLHDWAZQECBI-SRVKXCTJSA-N 0.000 claims description 3
BGOWRLSWJCVYAQ-CIUDSAMLSA-N Ser-Asp-Leu Chemical compound [H]N[C@@H](CO)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC(C)C)C(O)=O BGOWRLSWJCVYAQ-CIUDSAMLSA-N 0.000 claims description 3
YMTLKLXDFCSCNX-BYPYZUCNSA-N Ser-Gly-Gly Chemical compound OC[C@H](N)C(=O)NCC(=O)NCC(O)=O YMTLKLXDFCSCNX-BYPYZUCNSA-N 0.000 claims description 3
OWCVUSJMEBGMOK-YUMQZZPRSA-N Ser-Lys-Gly Chemical compound [H]N[C@@H](CO)C(=O)N[C@@H](CCCCN)C(=O)NCC(O)=O OWCVUSJMEBGMOK-YUMQZZPRSA-N 0.000 claims description 3
OUUQCZGPVNCOIJ-UHFFFAOYSA-M Superoxide Chemical compound [O-][O] OUUQCZGPVNCOIJ-UHFFFAOYSA-M 0.000 claims description 3
108010046334 Urease Proteins 0.000 claims description 3
230000005764 inhibitory process Effects 0.000 claims description 3
108010012058 leucyltyrosine Proteins 0.000 claims description 3
230000001105 regulatory effect Effects 0.000 claims description 3
HDHZCEDPLTVHFZ-GUBZILKMSA-N Asn-Leu-Glu Chemical compound [H]N[C@@H](CC(N)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(O)=O)C(O)=O HDHZCEDPLTVHFZ-GUBZILKMSA-N 0.000 claims description 2
AAKRWBIIGKPOKQ-ONGXEEELSA-N Leu-Val-Gly Chemical compound CC(C)C[C@H](N)C(=O)N[C@@H](C(C)C)C(=O)NCC(O)=O AAKRWBIIGKPOKQ-ONGXEEELSA-N 0.000 claims description 2
XYSXOCIWCPFOCG-IHRRRGAJSA-N Pro-Leu-Leu Chemical compound [H]N1CCC[C@H]1C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(O)=O XYSXOCIWCPFOCG-IHRRRGAJSA-N 0.000 claims description 2
RUCNAYOMFXRIKJ-DCAQKATOSA-N Val-Ala-Lys Chemical compound CC(C)[C@H](N)C(=O)N[C@@H](C)C(=O)N[C@H](C(O)=O)CCCCN RUCNAYOMFXRIKJ-DCAQKATOSA-N 0.000 claims description 2
OQWNEUXPKHIEJO-NRPADANISA-N Val-Glu-Ser Chemical compound CC(C)[C@@H](C(=O)N[C@@H](CCC(=O)O)C(=O)N[C@@H](CO)C(=O)O)N OQWNEUXPKHIEJO-NRPADANISA-N 0.000 claims description 2
108010089975 arginyl-glycyl-aspartyl-serine Proteins 0.000 claims description 2
108010084389 glycyltryptophan Proteins 0.000 claims description 2
230000002401 inhibitory effect Effects 0.000 claims description 2
239000003550 marker Substances 0.000 claims description 2
108010061238 threonyl-glycine Proteins 0.000 claims description 2
230000002103 transcriptional effect Effects 0.000 claims description 2
VPZXBVLAVMBEQI-UHFFFAOYSA-N glycyl-DL-alpha-alanine Natural products OC(=O)C(C)NC(=O)CN VPZXBVLAVMBEQI-UHFFFAOYSA-N 0.000 claims 9
PASHZZBXZYEXFE-LSDHHAIUSA-N Gly-Trp-Pro Chemical compound C1C[C@@H](N(C1)C(=O)[C@H](CC2=CNC3=CC=CC=C32)NC(=O)CN)C(=O)O PASHZZBXZYEXFE-LSDHHAIUSA-N 0.000 claims 7
WSGXUIQTEZDVHJ-GARJFASQSA-N Leu-Ala-Pro Chemical compound CC(C)C[C@H](N)C(=O)N[C@@H](C)C(=O)N1CCC[C@@H]1C(O)=O WSGXUIQTEZDVHJ-GARJFASQSA-N 0.000 claims 7
IKKVASZHTMKJIR-ZKWXMUAHSA-N Ala-Asp-Val Chemical compound [H]N[C@@H](C)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](C(C)C)C(O)=O IKKVASZHTMKJIR-ZKWXMUAHSA-N 0.000 claims 5
VHAQSYHSDKERBS-XPUUQOCRSA-N Ala-Val-Gly Chemical compound C[C@H](N)C(=O)N[C@@H](C(C)C)C(=O)NCC(O)=O VHAQSYHSDKERBS-XPUUQOCRSA-N 0.000 claims 5
QNMKWNONJGKJJC-NHCYSSNCSA-N Asp-Leu-Val Chemical compound [H]N[C@@H](CC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](C(C)C)C(O)=O QNMKWNONJGKJJC-NHCYSSNCSA-N 0.000 claims 5
BUEFQXUHTUZXHR-LURJTMIESA-N Gly-Gly-Pro zwitterion Chemical compound NCC(=O)NCC(=O)N1CCC[C@H]1C(O)=O BUEFQXUHTUZXHR-LURJTMIESA-N 0.000 claims 5
DPUOLKQSMYLRDR-UBHSHLNASA-N Phe-Arg-Ala Chemical compound NC(N)=NCCC[C@@H](C(=O)N[C@@H](C)C(O)=O)NC(=O)[C@@H](N)CC1=CC=CC=C1 DPUOLKQSMYLRDR-UBHSHLNASA-N 0.000 claims 5
UWQJHXKARZWDIJ-ZLUOBGJFSA-N Ala-Ala-Cys Chemical compound C[C@H](N)C(=O)N[C@@H](C)C(=O)N[C@@H](CS)C(O)=O UWQJHXKARZWDIJ-ZLUOBGJFSA-N 0.000 claims 4
BTYTYHBSJKQBQA-GCJQMDKQSA-N Ala-Asp-Thr Chemical compound C[C@H]([C@@H](C(=O)O)NC(=O)[C@H](CC(=O)O)NC(=O)[C@H](C)N)O BTYTYHBSJKQBQA-GCJQMDKQSA-N 0.000 claims 4
YJHKTAMKPGFJCT-NRPADANISA-N Ala-Val-Glu Chemical compound [H]N[C@@H](C)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CCC(O)=O)C(O)=O YJHKTAMKPGFJCT-NRPADANISA-N 0.000 claims 4
HYQYLOSCICEYTR-YUMQZZPRSA-N Asn-Gly-Leu Chemical compound [H]N[C@@H](CC(N)=O)C(=O)NCC(=O)N[C@@H](CC(C)C)C(O)=O HYQYLOSCICEYTR-YUMQZZPRSA-N 0.000 claims 4
FOKISINOENBSDM-WLTAIBSBSA-N Gly-Thr-Tyr Chemical compound [H]NCC(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CC1=CC=C(O)C=C1)C(O)=O FOKISINOENBSDM-WLTAIBSBSA-N 0.000 claims 4
LVWIJITYHRZHBO-IXOXFDKPSA-N His-Leu-Thr Chemical compound [H]N[C@@H](CC1=CNC=N1)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H]([C@@H](C)O)C(O)=O LVWIJITYHRZHBO-IXOXFDKPSA-N 0.000 claims 4
JPCHYAUKOUGOIB-HJGDQZAQSA-N Met-Glu-Thr Chemical compound CSCC[C@H](N)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H]([C@@H](C)O)C(O)=O JPCHYAUKOUGOIB-HJGDQZAQSA-N 0.000 claims 4
HQTKVSCNCDLXSX-BQBZGAKWSA-N Ser-Arg-Gly Chemical compound [H]N[C@@H](CO)C(=O)N[C@@H](CCCNC(N)=N)C(=O)NCC(O)=O HQTKVSCNCDLXSX-BQBZGAKWSA-N 0.000 claims 4
LYGKYFKSZTUXGZ-ZDLURKLDSA-N Thr-Cys-Gly Chemical compound [H]N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CS)C(=O)NCC(O)=O LYGKYFKSZTUXGZ-ZDLURKLDSA-N 0.000 claims 4
XEVHXNLPUBVQEX-DVJZZOLTSA-N Thr-Trp-Gly Chemical compound C[C@H]([C@@H](C(=O)N[C@@H](CC1=CNC2=CC=CC=C21)C(=O)NCC(=O)O)N)O XEVHXNLPUBVQEX-DVJZZOLTSA-N 0.000 claims 4
MVYRJYISVJWKSX-KBPBESRZSA-N Tyr-His-Gly Chemical compound C1=CC(=CC=C1C[C@@H](C(=O)N[C@@H](CC2=CN=CN2)C(=O)NCC(=O)O)N)O MVYRJYISVJWKSX-KBPBESRZSA-N 0.000 claims 4
MIAZWUMFUURQNP-YDHLFZDLSA-N Val-Tyr-Asn Chemical compound CC(C)[C@@H](C(=O)N[C@@H](CC1=CC=C(C=C1)O)C(=O)N[C@@H](CC(=O)N)C(=O)O)N MIAZWUMFUURQNP-YDHLFZDLSA-N 0.000 claims 4
108010047857 aspartylglycine Proteins 0.000 claims 4
108010016616 cysteinylglycine Proteins 0.000 claims 4
108010040030 histidinoalanine Proteins 0.000 claims 4
108010053725 prolylvaline Proteins 0.000 claims 4
OTCJMMRQBVDQRK-DCAQKATOSA-N Arg-Asp-Leu Chemical compound [H]N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC(C)C)C(O)=O OTCJMMRQBVDQRK-DCAQKATOSA-N 0.000 claims 3
KLKHFFMNGWULBN-VKHMYHEASA-N Asn-Gly Chemical compound NC(=O)C[C@H](N)C(=O)NCC(O)=O KLKHFFMNGWULBN-VKHMYHEASA-N 0.000 claims 3
JZLFYAAGGYMRIK-BYULHYEWSA-N Asn-Val-Asp Chemical compound [H]N[C@@H](CC(N)=O)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CC(O)=O)C(O)=O JZLFYAAGGYMRIK-BYULHYEWSA-N 0.000 claims 3
WCORRBXVISTKQL-WHFBIAKZSA-N Gly-Ser-Ser Chemical compound NCC(=O)N[C@@H](CO)C(=O)N[C@@H](CO)C(O)=O WCORRBXVISTKQL-WHFBIAKZSA-N 0.000 claims 3
FXTUGWXZTFMTIV-GJZGRUSLSA-N Gly-Trp-Arg Chemical compound C1=CC=C2C(=C1)C(=CN2)C[C@@H](C(=O)N[C@@H](CCCN=C(N)N)C(=O)O)NC(=O)CN FXTUGWXZTFMTIV-GJZGRUSLSA-N 0.000 claims 3
NOQPTNXSGNPJNS-YUMQZZPRSA-N His-Asn-Gly Chemical compound [H]N[C@@H](CC1=CNC=N1)C(=O)N[C@@H](CC(N)=O)C(=O)NCC(O)=O NOQPTNXSGNPJNS-YUMQZZPRSA-N 0.000 claims 3
ZFNLIDNJUWNIJL-WDCWCFNPSA-N Leu-Glu-Thr Chemical compound [H]N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H]([C@@H](C)O)C(O)=O ZFNLIDNJUWNIJL-WDCWCFNPSA-N 0.000 claims 3
SBANPBVRHYIMRR-UHFFFAOYSA-N Leu-Ser-Pro Natural products CC(C)CC(N)C(=O)NC(CO)C(=O)N1CCCC1C(O)=O SBANPBVRHYIMRR-UHFFFAOYSA-N 0.000 claims 3
JGKHAFUAPZCCDU-BZSNNMDCSA-N Leu-Tyr-Leu Chemical compound CC(C)C[C@H]([NH3+])C(=O)N[C@H](C(=O)N[C@@H](CC(C)C)C([O-])=O)CC1=CC=C(O)C=C1 JGKHAFUAPZCCDU-BZSNNMDCSA-N 0.000 claims 3
RFQATBGBLDAKGI-VHSXEESVSA-N Lys-Gly-Pro Chemical compound C1C[C@@H](N(C1)C(=O)CNC(=O)[C@H](CCCCN)N)C(=O)O RFQATBGBLDAKGI-VHSXEESVSA-N 0.000 claims 3
APKRGYLBSCWJJP-FXQIFTODSA-N Pro-Ala-Asp Chemical compound [H]N1CCC[C@H]1C(=O)N[C@@H](C)C(=O)N[C@@H](CC(O)=O)C(O)=O APKRGYLBSCWJJP-FXQIFTODSA-N 0.000 claims 3
ICTZKEXYDDZZFP-SRVKXCTJSA-N Pro-Arg-Pro Chemical compound N([C@@H](CCCN=C(N)N)C(=O)N1[C@@H](CCC1)C(O)=O)C(=O)[C@@H]1CCCN1 ICTZKEXYDDZZFP-SRVKXCTJSA-N 0.000 claims 3
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0089—Oxidoreductases (1.) acting on superoxide as acceptor (1.15)
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
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- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
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- C12N9/503—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from viruses
- C12N9/506—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from viruses derived from RNA viruses
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/95—Fusion polypeptide containing a motif/fusion for degradation (ubiquitin fusions, PEST sequence)
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2770/24011—Flaviviridae
- C12N2770/24211—Hepacivirus, e.g. hepatitis C virus, hepatitis G virus
- C12N2770/24222—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

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Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Enzymes And Modification Thereof (AREA)
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Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
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IE112991A 1990-04-04 1991-04-04 Hepatitis c virus protease IE911129A1 (en)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
USUNITEDSTATESOFAMERICA04/04/19905
US50543390A	1990-04-04	1990-04-04

Publications (2)

Publication Number	Publication Date
IE84608B1 IE84608B1 (en)
IE911129A1 true IE911129A1 (en)	1991-10-09

Family

ID=24010295

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
IE112991A IE911129A1 (en)	1990-04-04	1991-04-04	Hepatitis c virus protease

Country Status (12)

Country	Link
US (7)	US5371017A (fr)
EP (2)	EP0527788B1 (fr)
JP (3)	JP3320411B2 (fr)
AT (2)	ATE433460T1 (fr)
AU (1)	AU7675491A (fr)
CA (1)	CA2079105C (fr)
DE (2)	DE69133402T2 (fr)
DK (1)	DK0527788T3 (fr)
ES (2)	ES2324597T3 (fr)
IE (1)	IE911129A1 (fr)
PL (1)	PL169273B1 (fr)
WO (1)	WO1991015575A1 (fr)

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1991
- 1991-04-04 DE DE69133402T patent/DE69133402T2/de not_active Expired - Lifetime
- 1991-04-04 EP EP91908105A patent/EP0527788B1/fr not_active Expired - Lifetime
- 1991-04-04 JP JP50763191A patent/JP3320411B2/ja not_active Expired - Lifetime
- 1991-04-04 CA CA002079105A patent/CA2079105C/fr not_active Expired - Lifetime
- 1991-04-04 DK DK91908105T patent/DK0527788T3/da active
- 1991-04-04 PL PL91296328A patent/PL169273B1/pl unknown
- 1991-04-04 DE DE69133617T patent/DE69133617D1/de not_active Expired - Lifetime
- 1991-04-04 ES ES02019415T patent/ES2324597T3/es not_active Expired - Lifetime
- 1991-04-04 AT AT02019415T patent/ATE433460T1/de not_active IP Right Cessation
- 1991-04-04 AT AT91908105T patent/ATE270326T1/de not_active IP Right Cessation
- 1991-04-04 IE IE112991A patent/IE911129A1/en not_active IP Right Cessation
- 1991-04-04 AU AU76754/91A patent/AU7675491A/en not_active Abandoned
- 1991-04-04 EP EP02019415A patent/EP1304335B1/fr not_active Expired - Lifetime
- 1991-04-04 ES ES91908105T patent/ES2219637T3/es not_active Expired - Lifetime
- 1991-04-04 WO PCT/US1991/002210 patent/WO1991015575A1/fr not_active Ceased
- 1991-04-04 US US07/680,296 patent/US5371017A/en not_active Expired - Lifetime
1994
- 1994-12-06 US US08350884 patent/US5585258C1/en not_active Expired - Lifetime
1995
- 1995-05-12 US US08440548 patent/US5597691C1/en not_active Expired - Lifetime
1996
- 1996-09-06 US US08/709,177 patent/US5885799A/en not_active Expired - Lifetime
- 1996-09-06 US US08/709,173 patent/US5712145A/en not_active Expired - Lifetime
2001
- 2001-06-11 JP JP2001176369A patent/JP3507045B2/ja not_active Expired - Lifetime
- 2001-06-18 US US09/884,455 patent/US20030064499A1/en not_active Abandoned
- 2001-06-18 US US09/884,456 patent/US20030027317A1/en not_active Abandoned
2003
- 2003-10-31 JP JP2003373722A patent/JP2004073212A/ja not_active Withdrawn

Also Published As

Publication number	Publication date
US20030027317A1 (en)	2003-02-06
JP2004073212A (ja)	2004-03-11
US5585258A (en)	1996-12-17
WO1991015575A1 (fr)	1991-10-17
ATE270326T1 (de)	2004-07-15
JPH05507612A (ja)	1993-11-04
ATE433460T1 (de)	2009-06-15
PL169273B1 (pl)	1996-06-28
DE69133617D1 (de)	2009-07-23
ES2219637T3 (es)	2004-12-01
US5597691C1 (en)	2001-12-11
US5885799A (en)	1999-03-23
CA2079105A1 (fr)	1991-10-05
DE69133402T2 (de)	2004-11-11
US5597691A (en)	1997-01-28
CA2079105C (fr)	2000-06-13
AU7675491A (en)	1991-10-30
DE69133402D1 (de)	2004-08-12
US5371017A (en)	1994-12-06
EP1304335A3 (fr)	2004-04-07
JP2002051791A (ja)	2002-02-19
JP3507045B2 (ja)	2004-03-15
EP0527788B1 (fr)	2004-06-30
JP3320411B2 (ja)	2002-09-03
US5585258C1 (en)	2002-01-15
EP1304335B1 (fr)	2009-06-10
IE20060594A1 (en)	2009-05-27
US5712145A (en)	1998-01-27
EP0527788A1 (fr)	1993-02-24
EP1304335A2 (fr)	2003-04-23
DK0527788T3 (da)	2004-09-06
US20030064499A1 (en)	2003-04-03
ES2324597T3 (es)	2009-08-11

Legal Events

Date	Code	Title	Description
2011-05-25	MK9A	Patent expired

Publication	Publication Date	Title
US5585258A (en)	1996-12-17	Hepatitis C virus protease
US5989905A (en)	1999-11-23	HCV NS3 protein fragments having helicase activity and improved solubility
IE84608B1 (en)		Hepatitis C virus protease
Falgout et al.	1993	Deletion analysis of dengue virus type 4 nonstructural protein NS2B: identification of a domain required for NS2B-NS3 protease activity
US6211338B1 (en)	2001-04-03	Single-chain recombinant complexes of hepatitis C virus NS3 protease and NS4A cofactor peptide
EP0846164B1 (fr)	2005-03-02	Procede pour tester l'activite proteolytique de polypeptides ayant une activite de protease hcv ns3 et peptides substrats a utiliser dans cette methode.
WO1991015596A1 (fr)	1991-10-17	Inhibiteurs de la protease du virus de l'hepatite c
WO1997040168A1 (fr)	1997-10-30	Protease du virus de l'hepatite g
IE85503B1 (en)	2010-05-12	Hepatitis c virus protease
JPH05192160A (ja)	1993-08-03	Ｃ型肝炎ウイルスゲノムＲＮＡ、ｃＤＮＡ及びポリペプチド