IL100828A - Method and means for density gradient centrifugation - Google Patents

Method and means for density gradient centrifugation

Info

Publication number: IL100828A
Authority: IL; Israel
Prior art keywords: centrifugation; insert; vessel; layering; layering insert
Prior art date: 1992-01-31

Application number

IL10082892A

Other languages

English (en)

Other versions

IL100828A0 (en

Inventor

Michael Inbar

Mordechai Raduschetzky

Gerald Slutzky

David Shitrit

Original Assignee

Novamed Ltd

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1992-01-31

Filing date

1992-01-31

Publication date

2002-05-23

1992-01-31 Application filed by Novamed Ltd filed Critical Novamed Ltd

1992-01-31 Priority to IL10082892A priority Critical patent/IL100828A/en

1992-09-06 Publication of IL100828A0 publication Critical patent/IL100828A0/xx

1992-11-03 Priority to US07/971,525 priority patent/US5314074A/en

1992-12-10 Priority to AT92311300T priority patent/ATE155582T1/de

1992-12-10 Priority to DE69220917T priority patent/DE69220917T2/de

1992-12-10 Priority to ES92311300T priority patent/ES2108733T3/es

1992-12-10 Priority to EP92311300A priority patent/EP0553554B1/de

1992-12-16 Priority to JP33623992A priority patent/JP3325620B2/ja

2002-05-23 Publication of IL100828A publication Critical patent/IL100828A/en

Links

238000000432 density-gradient centrifugation Methods 0.000 title claims abstract description 11
238000000034 method Methods 0.000 title claims description 21
238000005119 centrifugation Methods 0.000 claims abstract description 52
239000007788 liquid Substances 0.000 claims abstract description 26
239000006185 dispersion Substances 0.000 claims abstract description 24
230000005484 gravity Effects 0.000 claims abstract description 8
239000012530 fluid Substances 0.000 claims description 20
239000007787 solid Substances 0.000 claims description 8
238000007373 indentation Methods 0.000 claims description 6
238000007789 sealing Methods 0.000 claims description 5
238000011084 recovery Methods 0.000 claims description 2
210000004369 blood Anatomy 0.000 description 6
239000008280 blood Substances 0.000 description 6
210000003743 erythrocyte Anatomy 0.000 description 6
239000000306 component Substances 0.000 description 5
210000004027 cell Anatomy 0.000 description 4
239000004033 plastic Substances 0.000 description 4
229920003023 plastic Polymers 0.000 description 4
239000013049 sediment Substances 0.000 description 4
239000004744 fabric Substances 0.000 description 3
210000000265 leukocyte Anatomy 0.000 description 3
239000007791 liquid phase Substances 0.000 description 3
239000000463 material Substances 0.000 description 3
-1 polyethylene Polymers 0.000 description 3
229920001917 Ficoll Polymers 0.000 description 2
239000004743 Polypropylene Substances 0.000 description 2
230000002457 bidirectional effect Effects 0.000 description 2
238000010908 decantation Methods 0.000 description 2
230000004048 modification Effects 0.000 description 2
238000012986 modification Methods 0.000 description 2
229920001155 polypropylene Polymers 0.000 description 2
238000000926 separation method Methods 0.000 description 2
239000004698 Polyethylene Substances 0.000 description 1
FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
241000700605 Viruses Species 0.000 description 1
XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 1
229910052782 aluminium Inorganic materials 0.000 description 1
239000012620 biological material Substances 0.000 description 1
230000015572 biosynthetic process Effects 0.000 description 1
239000012503 blood component Substances 0.000 description 1
210000003850 cellular structure Anatomy 0.000 description 1
238000004440 column chromatography Methods 0.000 description 1
238000010790 dilution Methods 0.000 description 1
239000012895 dilution Substances 0.000 description 1
239000000835 fiber Substances 0.000 description 1
150000004676 glycans Chemical class 0.000 description 1
238000003780 insertion Methods 0.000 description 1
230000037431 insertion Effects 0.000 description 1
230000005865 ionizing radiation Effects 0.000 description 1
239000000644 isotonic solution Substances 0.000 description 1
229920002521 macromolecule Polymers 0.000 description 1
238000004519 manufacturing process Methods 0.000 description 1
239000012528 membrane Substances 0.000 description 1
210000005087 mononuclear cell Anatomy 0.000 description 1
230000035515 penetration Effects 0.000 description 1
239000012071 phase Substances 0.000 description 1
230000000704 physical effect Effects 0.000 description 1
210000002381 plasma Anatomy 0.000 description 1
210000004623 platelet-rich plasma Anatomy 0.000 description 1
229920000573 polyethylene Polymers 0.000 description 1
229920001282 polysaccharide Polymers 0.000 description 1
239000005017 polysaccharide Substances 0.000 description 1
230000002265 prevention Effects 0.000 description 1
238000000746 purification Methods 0.000 description 1
238000004062 sedimentation Methods 0.000 description 1
239000011780 sodium chloride Substances 0.000 description 1
239000000243 solution Substances 0.000 description 1
239000006228 supernatant Substances 0.000 description 1
229920002994 synthetic fiber Polymers 0.000 description 1
239000012209 synthetic fiber Substances 0.000 description 1
235000001892 vitamin D2 Nutrition 0.000 description 1

Classifications

- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/483—Physical analysis of biological material
- G01N33/487—Physical analysis of biological material of liquid biological material
- G01N33/49—Blood
- G01N33/491—Blood by separating the blood components

Landscapes

Health & Medical Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Engineering & Computer Science (AREA)
Biomedical Technology (AREA)
Chemical & Material Sciences (AREA)
Hematology (AREA)
Physics & Mathematics (AREA)
Analytical Chemistry (AREA)
Biochemistry (AREA)
Urology & Nephrology (AREA)
Ecology (AREA)
Food Science & Technology (AREA)
Medicinal Chemistry (AREA)
Biophysics (AREA)
Molecular Biology (AREA)
General Health & Medical Sciences (AREA)
General Physics & Mathematics (AREA)
Immunology (AREA)
Pathology (AREA)
Centrifugal Separators (AREA)
Sampling And Sample Adjustment (AREA)
Investigating Or Analysing Biological Materials (AREA)
External Artificial Organs (AREA)

IL10082892A 1992-01-31 1992-01-31 Method and means for density gradient centrifugation IL100828A (en)

Priority Applications (7)

Application Number	Priority Date	Filing Date	Title
IL10082892A IL100828A (en)	1992-01-31	1992-01-31	Method and means for density gradient centrifugation
US07/971,525 US5314074A (en)	1992-01-31	1992-11-03	Method and means for density gradient centrifugation
AT92311300T ATE155582T1 (de)	1992-01-31	1992-12-10	Verfahren und vorrichtung zur zentrifugaltrennung aufgrund des dichtegradienten
DE69220917T DE69220917T2 (de)	1992-01-31	1992-12-10	Verfahren und Vorrichtung zur Zentrifugaltrennung aufgrund des Dichtegradienten
ES92311300T ES2108733T3 (es)	1992-01-31	1992-12-10	Metodo y medios para centrifugacion por gradiente de densidad.
EP92311300A EP0553554B1 (de)	1992-01-31	1992-12-10	Verfahren und Vorrichtung zur Zentrifugaltrennung aufgrund des Dichtegradienten
JP33623992A JP3325620B2 (ja)	1992-01-31	1992-12-16	密度勾配遠心分離を実施する方法と、それに使用する成層用インサート等

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
IL10082892A IL100828A (en)	1992-01-31	1992-01-31	Method and means for density gradient centrifugation

Publications (2)

Publication Number	Publication Date
IL100828A0 IL100828A0 (en)	1992-09-06
IL100828A true IL100828A (en)	2002-05-23

Family

ID=11063334

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
IL10082892A IL100828A (en)	1992-01-31	1992-01-31	Method and means for density gradient centrifugation

Country Status (7)

Country	Link
US (1)	US5314074A (de)
EP (1)	EP0553554B1 (de)
JP (1)	JP3325620B2 (de)
AT (1)	ATE155582T1 (de)
DE (1)	DE69220917T2 (de)
ES (1)	ES2108733T3 (de)
IL (1)	IL100828A (de)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US5431814A (en) *	1993-10-22	1995-07-11	Jorgensen; Glen	Centrifugal filter apparatus and method
US5648223A (en) *	1994-08-31	1997-07-15	Activated Cell Therapy, Inc.	Methods for enriching breast tumor cells
US5646004A (en) *	1994-08-31	1997-07-08	Activated Cell Therapy, Inc.	Methods for enriching fetal cells from maternal body fluids
JP3397795B2 (ja) *	1994-08-31	2003-04-21	デンドレオンコーポレイション	細胞分離装置および方法
US5707331A (en) *	1995-05-05	1998-01-13	John R. Wells	Automatic multiple-decanting centrifuge
AUPO488697A0 (en) *	1997-01-31	1997-02-20	Australian Red Cross Society (Western Australian Division)	Method and means for separating blood
US6390966B2 (en) *	2000-04-18	2002-05-21	Large Scale Proteomics Corporation	Method for making density gradients
DE60132198T2 (de)	2000-04-28	2008-12-18	Harvest Technologies Corp., Plymouth	Plattentrenneinrichtung für blutbestandteile
MX2009009506A (es) *	2007-03-23	2009-09-16	Nestec Sa	Capsula de ingredientes para bebida con placa perforada que tiene perforaciones liberadoras de presion.
US8177072B2 (en) *	2008-12-04	2012-05-15	Thermogenesis Corp.	Apparatus and method for separating and isolating components of a biological fluid
KR101197974B1 (ko) *	2011-11-01	2012-11-05	박현정	신속한 원심분리가 가능한 원심분리용 용기
GB201418382D0 (en) *	2014-10-16	2014-12-03	Ge Healthcare Uk Ltd	Device and method for separating cells
CN104593221B (zh) *	2015-01-13	2016-10-19	中国科学院生物物理研究所	可调多层细胞分离筛装置
WO2017046736A1 (en) *	2015-09-15	2017-03-23	Spark-Tech Sp. Z O.O.	Device and method for fluids separation by density gradient
PL237582B1 (pl) *	2015-09-15	2021-05-04	Spark Tech Spolka Z Ograniczona Odpowiedzialnoscia	Insert do pojemnika do wirowania, zwłaszcza, do probówki, do wydzielenia frakcji o pożądanym zakresie gęstości metodą wirowania na gradiencie gęstości oraz pojemnik do wirowania zawierający ten insert
JP7733073B2 (ja) *	2023-07-18	2025-09-02	Ｈ．Ｕ．グループホールディングス株式会社	分離インサート、及び分離器具
CN118086051A (zh) *	2024-04-10	2024-05-28	福建省妇幼保健院	一种可充分灵活回收活动精子的精子上游管

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US4001122A (en) *	1973-08-22	1977-01-04	Telan Corporation	Method and device for separating blood components
US3931010A (en) *	1974-02-27	1976-01-06	Becton, Dickinson And Company	Serum/plasma separators with centrifugal valves
US4083788A (en) *	1975-11-19	1978-04-11	Ferrara Louis T	Blood serum-isolation device
US4257886A (en) *	1979-01-18	1981-03-24	Becton, Dickinson And Company	Apparatus for the separation of blood components
US4224942A (en) *	1979-04-25	1980-09-30	Northwestern University	Cell fractionating method
US4417981A (en) *	1981-05-04	1983-11-29	Becton, Dickinson And Company	Blood phase separator device
US4492634A (en) *	1982-09-28	1985-01-08	Emde Medical Research	Pre-evacuated blood collection tube with anti-hemolysis baffle system and centrifugation propelled filtration disc and efficient serum-from cells separator
CA1291098C (en) *	1984-12-04	1991-10-22	Albert August Luderer	Lymphocyte collection tube
US5053134A (en) *	1984-12-04	1991-10-01	Becton Dickinson And Company	Lymphocyte collection tube
US4722792A (en) *	1985-02-09	1988-02-02	Kurashiki Boseki Kabushiki Kaisha	Filter for centrifugal separator
IL74967A (en) *	1985-04-18	1988-10-31	Assaf Pharmaceutical Ind	Separation of materials from a liquid dispersion by sedimentation
US4602995A (en) *	1985-05-20	1986-07-29	Technicon Instruments Corporation	Liquid level adjusting and filtering device
US4636361A (en) *	1985-11-18	1987-01-13	Miklos Marian	Device for separating liquid fractions
US4683058A (en) *	1986-03-20	1987-07-28	Costar Corporation	Filter for centrifuge tube
US4822495A (en) *	1987-07-07	1989-04-18	St. Mary's Hospital And Medical Center	Cell block collection method and apparatus
US4818386A (en) *	1987-10-08	1989-04-04	Becton, Dickinson And Company	Device for separating the components of a liquid sample having higher and lower specific gravities
US4844818A (en) *	1987-10-23	1989-07-04	Becton Dickinson & Company	Method for separating the cellular components of blood samples

1992
- 1992-01-31 IL IL10082892A patent/IL100828A/en not_active IP Right Cessation
- 1992-11-03 US US07/971,525 patent/US5314074A/en not_active Expired - Fee Related
- 1992-12-10 DE DE69220917T patent/DE69220917T2/de not_active Expired - Fee Related
- 1992-12-10 AT AT92311300T patent/ATE155582T1/de not_active IP Right Cessation
- 1992-12-10 ES ES92311300T patent/ES2108733T3/es not_active Expired - Lifetime
- 1992-12-10 EP EP92311300A patent/EP0553554B1/de not_active Expired - Lifetime
- 1992-12-16 JP JP33623992A patent/JP3325620B2/ja not_active Expired - Lifetime

Also Published As

Publication number	Publication date
EP0553554B1 (de)	1997-07-16
EP0553554A1 (de)	1993-08-04
DE69220917T2 (de)	1998-09-10
JPH06206008A (ja)	1994-07-26
JP3325620B2 (ja)	2002-09-17
DE69220917D1 (de)	1997-08-21
ATE155582T1 (de)	1997-08-15
US5314074A (en)	1994-05-24
ES2108733T3 (es)	1998-01-01
IL100828A0 (en)	1992-09-06

Legal Events

Date	Code	Title
2002-11-10	FF	Patent granted
2002-12-01	KB	Patent renewed
2006-03-12	KB	Patent renewed
2010-06-16	KB	Patent renewed
2012-10-31	EXP	Patent expired

Publication	Publication Date	Title
US5314074A (en)	1994-05-24	Method and means for density gradient centrifugation
US4861477A (en)	1989-08-29	Tubular container for centrifugal separation
US5560830A (en)	1996-10-01	Separator float and tubular body for blood collection and separation and method of use thereof
EP1014088B1 (de)	2006-03-08	Vorrichtung und Verfahren zur Trennung von Bestanteilen einer flüssigen Probe
US4040959A (en)	1977-08-09	Multi-purpose blood bag
US7205157B2 (en)	2007-04-17	Method of separating cells from a sample
US6406671B1 (en)	2002-06-18	Device and method for separating components of a fluid sample
US4436631A (en)	1984-03-13	Multiple particle washing system and method of use
CA1296693C (en)	1992-03-03	Apparatus and method for separating phases of blood
US3879295A (en)	1975-04-22	Vacutainer with positive separation barrier
US5707876A (en)	1998-01-13	Method and apparatus for harvesting constituent layers from a centrifuged material mixture
US4892537A (en)	1990-01-09	Bag for separation and isolation of blood components
AU5156193A (en)	1993-11-29	Flow restrictor-separation device
GB1592935A (en)	1981-07-15	Device for use in the centrifugal separation of components of a liquid
SK138394A3 (en)	1995-06-07	Method for separating of liquid sample and device for realization of this method
US11534534B2 (en)	2022-12-27	Apparatus and methods for processing blood
US12097311B2 (en)	2024-09-24	Apparatus and methods for concentrating platelet-rich plasma
US20200064329A1 (en)	2020-02-27	Blood Plasma Separation Device
CN106796216B (zh)	2020-03-03	用于分离细胞的装置和方法
US4969882A (en)	1990-11-13	Bag for separation and isolation of blood components
US20230010728A1 (en)	2023-01-12	Device and Method for Centrifuging a Physiological Fluid
IL106488A (en)	1996-08-04	Method for density gradient centrifugation
Murthy et al.	1980	Recovery of microquantities of subcellular particles by ultracentrifugation in automatic pipet tips