IL108330A - Method for preparing somatic embryos from a medium of suspension culture of plant tissue and preparation of plants from the above embryos - Google Patents

Method for preparing somatic embryos from a medium of suspension culture of plant tissue and preparation of plants from the above embryos

Info

Publication number: IL108330A
Authority: IL; Israel
Prior art keywords: pems; somatic embryos; pem; medium; embryos
Prior art date: 1993-01-15

Application number

IL10833094A

Other languages

English (en)

Hebrew (he)

Other versions

IL108330A0 (en

Original Assignee

Novartis Seeds B V

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1993-01-15

Filing date

1994-01-13

Publication date

2004-02-19

Family has litigation

First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=27450981&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=IL108330(A) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.

1993-01-15 Priority claimed from GB939300706A external-priority patent/GB9300706D0/en

1993-01-15 Priority claimed from GB939300705A external-priority patent/GB9300705D0/en

1993-01-15 Priority claimed from GB939300707A external-priority patent/GB9300707D0/en

1993-01-15 Priority claimed from GB939300729A external-priority patent/GB9300729D0/en

1994-01-13 Application filed by Novartis Seeds B V filed Critical Novartis Seeds B V

1994-04-12 Publication of IL108330A0 publication Critical patent/IL108330A0/xx

2004-02-19 Publication of IL108330A publication Critical patent/IL108330A/en

Links

210000002257 embryonic structure Anatomy 0.000 title claims abstract description 130
230000000392 somatic effect Effects 0.000 title claims abstract description 122
238000000034 method Methods 0.000 title claims abstract description 68
238000004114 suspension culture Methods 0.000 title claims abstract description 32
239000001963 growth medium Substances 0.000 title description 8
238000002360 preparation method Methods 0.000 title description 4
230000000408 embryogenic effect Effects 0.000 claims abstract description 35
210000001161 mammalian embryo Anatomy 0.000 claims abstract description 20
241000196324 Embryophyta Species 0.000 claims description 84
239000002609 medium Substances 0.000 claims description 82
SEOVTRFCIGRIMH-UHFFFAOYSA-N indole-3-acetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CNC2=C1 SEOVTRFCIGRIMH-UHFFFAOYSA-N 0.000 claims description 73
229930192334 Auxin Natural products 0.000 claims description 71
239000002363 auxin Substances 0.000 claims description 71
239000007788 liquid Substances 0.000 claims description 55
239000000463 material Substances 0.000 claims description 54
238000004161 plant tissue culture Methods 0.000 claims description 43
239000003104 tissue culture media Substances 0.000 claims description 41
CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 35
229930006000 Sucrose Natural products 0.000 claims description 35
239000005720 sucrose Substances 0.000 claims description 35
238000012258 culturing Methods 0.000 claims description 30
230000015572 biosynthetic process Effects 0.000 claims description 25
206010020649 Hyperkeratosis Diseases 0.000 claims description 24
208000035199 Tetraploidy Diseases 0.000 claims description 20
239000000725 suspension Substances 0.000 claims description 18
241000612153 Cyclamen Species 0.000 claims description 12
229930186364 cyclamen Natural products 0.000 claims description 11
230000006698 induction Effects 0.000 claims description 10
239000000203 mixture Substances 0.000 claims description 10
241000227653 Lycopersicon Species 0.000 claims description 8
150000001720 carbohydrates Chemical class 0.000 claims description 8
241000208175 Daucus Species 0.000 claims description 6
WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 6
241000208181 Pelargonium Species 0.000 claims description 6
WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 claims description 6
239000008103 glucose Substances 0.000 claims description 6
235000002566 Capsicum Nutrition 0.000 claims description 5
241000405217 Viola <butterfly> Species 0.000 claims description 5
235000002262 Lycopersicon Nutrition 0.000 claims description 4
241000219198 Brassica Species 0.000 claims description 3
235000011331 Brassica Nutrition 0.000 claims description 3
240000008574 Capsicum frutescens Species 0.000 claims description 3
MUPFEKGTMRGPLJ-RMMQSMQOSA-N Raffinose Natural products O(C[C@H]1[C@@H](O)[C@H](O)[C@@H](O)[C@@H](O[C@@]2(CO)[C@H](O)[C@@H](O)[C@@H](CO)O2)O1)[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 MUPFEKGTMRGPLJ-RMMQSMQOSA-N 0.000 claims description 3
MUPFEKGTMRGPLJ-UHFFFAOYSA-N UNPD196149 Natural products OC1C(O)C(CO)OC1(CO)OC1C(O)C(O)C(O)C(COC2C(C(O)C(O)C(CO)O2)O)O1 MUPFEKGTMRGPLJ-UHFFFAOYSA-N 0.000 claims description 3
230000001413 cellular effect Effects 0.000 claims description 3
210000001672 ovary Anatomy 0.000 claims description 3
MUPFEKGTMRGPLJ-ZQSKZDJDSA-N raffinose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO[C@@H]2[C@@H]([C@@H](O)[C@@H](O)[C@@H](CO)O2)O)O1 MUPFEKGTMRGPLJ-ZQSKZDJDSA-N 0.000 claims description 3
230000002792 vascular Effects 0.000 claims description 3
241000234282 Allium Species 0.000 claims description 2
241000218999 Begoniaceae Species 0.000 claims description 2
241000723343 Cichorium Species 0.000 claims description 2
241000735332 Gerbera Species 0.000 claims description 2
241001495448 Impatiens <genus> Species 0.000 claims description 2
241000208822 Lactuca Species 0.000 claims description 2
241000209094 Oryza Species 0.000 claims description 2
240000007377 Petunia x hybrida Species 0.000 claims description 2
241000245063 Primula Species 0.000 claims description 2
235000000497 Primula Nutrition 0.000 claims description 2
229930190166 impatien Natural products 0.000 claims description 2
210000001938 protoplast Anatomy 0.000 claims description 2
238000002047 photoemission electron microscopy Methods 0.000 claims 7
229920001483 poly(ethyl methacrylate) polymer Polymers 0.000 claims 7
239000005631 2,4-Dichlorophenoxyacetic acid Substances 0.000 description 49
210000004027 cell Anatomy 0.000 description 49
FAIXYKHYOGVFKA-UHFFFAOYSA-N Kinetin Natural products N=1C=NC=2N=CNC=2C=1N(C)C1=CC=CO1 FAIXYKHYOGVFKA-UHFFFAOYSA-N 0.000 description 33
QANMHLXAZMSUEX-UHFFFAOYSA-N kinetin Chemical compound N=1C=NC=2N=CNC=2C=1NCC1=CC=CO1 QANMHLXAZMSUEX-UHFFFAOYSA-N 0.000 description 33
229960001669 kinetin Drugs 0.000 description 33
LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 27
210000001519 tissue Anatomy 0.000 description 19
240000008067 Cucumis sativus Species 0.000 description 18
PVNIIMVLHYAWGP-UHFFFAOYSA-N Niacin Chemical compound OC(=O)C1=CC=CN=C1 PVNIIMVLHYAWGP-UHFFFAOYSA-N 0.000 description 18
235000010799 Cucumis sativus var sativus Nutrition 0.000 description 15
239000002028 Biomass Substances 0.000 description 14
239000000243 solution Substances 0.000 description 14
239000006870 ms-medium Substances 0.000 description 13
230000030118 somatic embryogenesis Effects 0.000 description 13
QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 12
229910052760 oxygen Inorganic materials 0.000 description 12
239000001301 oxygen Substances 0.000 description 12
238000007873 sieving Methods 0.000 description 12
238000011161 development Methods 0.000 description 11
230000018109 developmental process Effects 0.000 description 11
LXNHXLLTXMVWPM-UHFFFAOYSA-N pyridoxine Chemical compound CC1=NC=C(CO)C(CO)=C1O LXNHXLLTXMVWPM-UHFFFAOYSA-N 0.000 description 11
PRPINYUDVPFIRX-UHFFFAOYSA-N 1-naphthaleneacetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CC=CC2=C1 PRPINYUDVPFIRX-UHFFFAOYSA-N 0.000 description 10
239000004677 Nylon Substances 0.000 description 10
UQHKFADEQIVWID-UHFFFAOYSA-N cytokinin Natural products C1=NC=2C(NCC=C(CO)C)=NC=NC=2N1C1CC(O)C(CO)O1 UQHKFADEQIVWID-UHFFFAOYSA-N 0.000 description 10
239000004062 cytokinin Substances 0.000 description 10
229920001778 nylon Polymers 0.000 description 10
239000005708 Sodium hypochlorite Substances 0.000 description 9
230000000977 initiatory effect Effects 0.000 description 9
CDAISMWEOUEBRE-GPIVLXJGSA-N inositol Chemical compound O[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@H](O)[C@@H]1O CDAISMWEOUEBRE-GPIVLXJGSA-N 0.000 description 9
239000011664 nicotinic acid Substances 0.000 description 9
229960003512 nicotinic acid Drugs 0.000 description 9
235000001968 nicotinic acid Nutrition 0.000 description 9
239000011148 porous material Substances 0.000 description 9
SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 description 9
239000008223 sterile water Substances 0.000 description 9
238000003756 stirring Methods 0.000 description 9
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 9
229960000367 inositol Drugs 0.000 description 8
CDAISMWEOUEBRE-UHFFFAOYSA-N scyllo-inosotol Natural products OC1C(O)C(O)C(O)C(O)C1O CDAISMWEOUEBRE-UHFFFAOYSA-N 0.000 description 8
229960003495 thiamine Drugs 0.000 description 8
229940088594 vitamin Drugs 0.000 description 8
229930003231 vitamin Natural products 0.000 description 8
235000013343 vitamin Nutrition 0.000 description 8
239000011782 vitamin Substances 0.000 description 8
229940011671 vitamin b6 Drugs 0.000 description 8
238000007865 diluting Methods 0.000 description 7
239000012737 fresh medium Substances 0.000 description 7
DPJRMOMPQZCRJU-UHFFFAOYSA-M thiamine hydrochloride Chemical compound Cl.[Cl-].CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N DPJRMOMPQZCRJU-UHFFFAOYSA-M 0.000 description 7
OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 6
229910052799 carbon Inorganic materials 0.000 description 6
239000006285 cell suspension Substances 0.000 description 6
KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 6
230000013020 embryo development Effects 0.000 description 6
230000012010 growth Effects 0.000 description 6
239000007787 solid Substances 0.000 description 6
235000014633 carbohydrates Nutrition 0.000 description 5
238000006243 chemical reaction Methods 0.000 description 5
239000003795 chemical substances by application Substances 0.000 description 5
238000004128 high performance liquid chromatography Methods 0.000 description 5
230000001737 promoting effect Effects 0.000 description 5
235000019171 pyridoxine hydrochloride Nutrition 0.000 description 5
239000011764 pyridoxine hydrochloride Substances 0.000 description 5
241000219310 Beta vulgaris subsp. vulgaris Species 0.000 description 4
235000007688 Lycopersicon esculentum Nutrition 0.000 description 4
235000021536 Sugar beet Nutrition 0.000 description 4
239000012530 fluid Substances 0.000 description 4
230000002068 genetic effect Effects 0.000 description 4
230000001939 inductive effect Effects 0.000 description 4
241000894007 species Species 0.000 description 4
238000010561 standard procedure Methods 0.000 description 4
239000011550 stock solution Substances 0.000 description 4
BUCXEFZXWKUCCY-UHFFFAOYSA-N 4-methyl-3-(2-phenylethyl)-1,2,4-oxadiazol-5-one Chemical compound O1C(=O)N(C)C(CCC=2C=CC=CC=2)=N1 BUCXEFZXWKUCCY-UHFFFAOYSA-N 0.000 description 3
244000000626 Daucus carota Species 0.000 description 3
235000002767 Daucus carota Nutrition 0.000 description 3
241001415771 Torpedinidae Species 0.000 description 3
238000004458 analytical method Methods 0.000 description 3
238000004113 cell culture Methods 0.000 description 3
238000004519 manufacturing process Methods 0.000 description 3
238000005259 measurement Methods 0.000 description 3
230000000442 meristematic effect Effects 0.000 description 3
238000002229 photoelectron microspectroscopy Methods 0.000 description 3
235000008160 pyridoxine Nutrition 0.000 description 3
239000011677 pyridoxine Substances 0.000 description 3
239000002689 soil Substances 0.000 description 3
238000012360 testing method Methods 0.000 description 3
210000003934 vacuole Anatomy 0.000 description 3
229920001817 Agar Polymers 0.000 description 2
239000006002 Pepper Substances 0.000 description 2
235000016761 Piper aduncum Nutrition 0.000 description 2
235000017804 Piper guineense Nutrition 0.000 description 2
244000203593 Piper nigrum Species 0.000 description 2
235000008184 Piper nigrum Nutrition 0.000 description 2
239000008272 agar Substances 0.000 description 2
239000005030 aluminium foil Substances 0.000 description 2
HUTDUHSNJYTCAR-UHFFFAOYSA-N ancymidol Chemical compound C1=CC(OC)=CC=C1C(O)(C=1C=NC=NC=1)C1CC1 HUTDUHSNJYTCAR-UHFFFAOYSA-N 0.000 description 2
150000001875 compounds Chemical class 0.000 description 2
238000000151 deposition Methods 0.000 description 2
239000012895 dilution Substances 0.000 description 2
238000010790 dilution Methods 0.000 description 2
235000013399 edible fruits Nutrition 0.000 description 2
230000000694 effects Effects 0.000 description 2
238000005516 engineering process Methods 0.000 description 2
238000001914 filtration Methods 0.000 description 2
239000005556 hormone Substances 0.000 description 2
229940088597 hormone Drugs 0.000 description 2
230000001976 improved effect Effects 0.000 description 2
239000003617 indole-3-acetic acid Substances 0.000 description 2
JTEDVYBZBROSJT-UHFFFAOYSA-N indole-3-butyric acid Chemical compound C1=CC=C2C(CCCC(=O)O)=CNC2=C1 JTEDVYBZBROSJT-UHFFFAOYSA-N 0.000 description 2
238000011081 inoculation Methods 0.000 description 2
238000009630 liquid culture Methods 0.000 description 2
238000012544 monitoring process Methods 0.000 description 2
210000000056 organ Anatomy 0.000 description 2
239000010451 perlite Substances 0.000 description 2
235000019362 perlite Nutrition 0.000 description 2
238000003976 plant breeding Methods 0.000 description 2
239000003375 plant hormone Substances 0.000 description 2
238000004382 potting Methods 0.000 description 2
229940047208 pyridoxine 10 mg Drugs 0.000 description 2
239000007858 starting material Substances 0.000 description 2
229940088174 thiamine 1 mg Drugs 0.000 description 2
UZKQTCBAMSWPJD-UQCOIBPSSA-N trans-Zeatin Natural products OCC(/C)=C\CNC1=NC=NC2=C1N=CN2 UZKQTCBAMSWPJD-UQCOIBPSSA-N 0.000 description 2
UZKQTCBAMSWPJD-FARCUNLSSA-N trans-zeatin Chemical compound OCC(/C)=C/CNC1=NC=NC2=C1N=CN2 UZKQTCBAMSWPJD-FARCUNLSSA-N 0.000 description 2
238000012546 transfer Methods 0.000 description 2
229940023877 zeatin Drugs 0.000 description 2
HXKWSTRRCHTUEC-UHFFFAOYSA-N 2,4-Dichlorophenoxyaceticacid Chemical compound OC(=O)C(Cl)OC1=CC=C(Cl)C=C1 HXKWSTRRCHTUEC-UHFFFAOYSA-N 0.000 description 1
241000335053 Beta vulgaris Species 0.000 description 1
235000021533 Beta vulgaris Nutrition 0.000 description 1
240000002791 Brassica napus Species 0.000 description 1
240000007124 Brassica oleracea Species 0.000 description 1
UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 1
244000241257 Cucumis melo Species 0.000 description 1
235000009842 Cucumis melo Nutrition 0.000 description 1
235000009849 Cucumis sativus Nutrition 0.000 description 1
240000009034 Cyclamen persicum Species 0.000 description 1
SQUHHTBVTRBESD-UHFFFAOYSA-N Hexa-Ac-myo-Inositol Natural products CC(=O)OC1C(OC(C)=O)C(OC(C)=O)C(OC(C)=O)C(OC(C)=O)C1OC(C)=O SQUHHTBVTRBESD-UHFFFAOYSA-N 0.000 description 1
206010021033 Hypomenorrhoea Diseases 0.000 description 1
241000218922 Magnoliophyta Species 0.000 description 1
NWBJYWHLCVSVIJ-UHFFFAOYSA-N N-benzyladenine Chemical compound N=1C=NC=2NC=NC=2C=1NCC1=CC=CC=C1 NWBJYWHLCVSVIJ-UHFFFAOYSA-N 0.000 description 1
240000004713 Pisum sativum Species 0.000 description 1
235000010582 Pisum sativum Nutrition 0.000 description 1
JZRWCGZRTZMZEH-UHFFFAOYSA-N Thiamine Natural products CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N JZRWCGZRTZMZEH-UHFFFAOYSA-N 0.000 description 1
230000005856 abnormality Effects 0.000 description 1
229910052921 ammonium sulfate Inorganic materials 0.000 description 1
230000003698 anagen phase Effects 0.000 description 1
238000011138 biotechnological process Methods 0.000 description 1
239000001110 calcium chloride Substances 0.000 description 1
229910001628 calcium chloride Inorganic materials 0.000 description 1
235000011148 calcium chloride Nutrition 0.000 description 1
238000005119 centrifugation Methods 0.000 description 1
238000010367 cloning Methods 0.000 description 1
235000020197 coconut milk Nutrition 0.000 description 1
230000001332 colony forming effect Effects 0.000 description 1
210000000805 cytoplasm Anatomy 0.000 description 1
230000001086 cytosolic effect Effects 0.000 description 1
230000007423 decrease Effects 0.000 description 1
230000008021 deposition Effects 0.000 description 1
238000011156 evaluation Methods 0.000 description 1
238000000684 flow cytometry Methods 0.000 description 1
239000000499 gel Substances 0.000 description 1
230000035784 germination Effects 0.000 description 1
PEYVWSJAZONVQK-UHFFFAOYSA-N hydroperoxy(oxo)borane Chemical compound OOB=O PEYVWSJAZONVQK-UHFFFAOYSA-N 0.000 description 1
238000000338 in vitro Methods 0.000 description 1
229910017053 inorganic salt Inorganic materials 0.000 description 1
238000012423 maintenance Methods 0.000 description 1
239000002207 metabolite Substances 0.000 description 1
238000000386 microscopy Methods 0.000 description 1
238000002156 mixing Methods 0.000 description 1
229910000402 monopotassium phosphate Inorganic materials 0.000 description 1
230000000877 morphologic effect Effects 0.000 description 1
238000011206 morphological examination Methods 0.000 description 1
231100001184 nonphytotoxic Toxicity 0.000 description 1
230000000888 organogenic effect Effects 0.000 description 1
239000002243 precursor Substances 0.000 description 1
108090000623 proteins and genes Proteins 0.000 description 1
108010050466 raffinase Proteins 0.000 description 1
230000001172 regenerating effect Effects 0.000 description 1
238000011160 research Methods 0.000 description 1
230000000717 retained effect Effects 0.000 description 1
150000003839 salts Chemical class 0.000 description 1
238000012216 screening Methods 0.000 description 1
239000000126 substance Substances 0.000 description 1
229960004793 sucrose Drugs 0.000 description 1
230000009469 supplementation Effects 0.000 description 1
208000027223 tetraploidy syndrome Diseases 0.000 description 1
235000019157 thiamine Nutrition 0.000 description 1
KYMBYSLLVAOCFI-UHFFFAOYSA-N thiamine Chemical compound CC1=C(CCO)SCN1CC1=CN=C(C)N=C1N KYMBYSLLVAOCFI-UHFFFAOYSA-N 0.000 description 1
239000011721 thiamine Substances 0.000 description 1
231100000331 toxic Toxicity 0.000 description 1
230000002588 toxic effect Effects 0.000 description 1
238000013519 translation Methods 0.000 description 1
238000011282 treatment Methods 0.000 description 1
230000017260 vegetative to reproductive phase transition of meristem Effects 0.000 description 1
230000035899 viability Effects 0.000 description 1
230000003442 weekly effect Effects 0.000 description 1

Classifications

- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/005—Methods for micropropagation; Vegetative plant propagation using cell or tissue culture techniques
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/0018—Culture media for cell or tissue culture
- C12N5/0025—Culture media for plant cell or plant tissue culture
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/04—Plant cells or tissues

Landscapes

Life Sciences & Earth Sciences (AREA)
Engineering & Computer Science (AREA)
Biotechnology (AREA)
Health & Medical Sciences (AREA)
Biomedical Technology (AREA)
Cell Biology (AREA)
Developmental Biology & Embryology (AREA)
Organic Chemistry (AREA)
Chemical & Material Sciences (AREA)
Bioinformatics & Cheminformatics (AREA)
Genetics & Genomics (AREA)
Botany (AREA)
Wood Science & Technology (AREA)
Zoology (AREA)
General Engineering & Computer Science (AREA)
Biochemistry (AREA)
Environmental Sciences (AREA)
General Health & Medical Sciences (AREA)
Microbiology (AREA)
Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Medicines Containing Plant Substances (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)
Nitrogen Condensed Heterocyclic Rings (AREA)
Luminescent Compositions (AREA)
External Artificial Organs (AREA)

IL10833094A 1993-01-15 1994-01-13 Method for preparing somatic embryos from a medium of suspension culture of plant tissue and preparation of plants from the above embryos IL108330A (en)

Applications Claiming Priority (4)

Application Number	Priority Date	Filing Date	Title
GB939300706A GB9300706D0 (en)	1993-01-15	1993-01-15	Improvements in or relating to organic compounds
GB939300705A GB9300705D0 (en)	1993-01-15	1993-01-15	Improvements in or relating to organic compounds
GB939300707A GB9300707D0 (en)	1993-01-15	1993-01-15	Improvements in or relating to organic compounds
GB939300729A GB9300729D0 (en)	1993-01-15	1993-01-15	Improvements in or relating to organic compounds

Publications (2)

Publication Number	Publication Date
IL108330A0 IL108330A0 (en)	1994-04-12
IL108330A true IL108330A (en)	2004-02-19

Family

ID=27450981

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
IL10833094A IL108330A (en)	1993-01-15	1994-01-13	Method for preparing somatic embryos from a medium of suspension culture of plant tissue and preparation of plants from the above embryos

Country Status (18)

Country	Link
US (1)	US5587312A (de)
EP (2)	EP0608716B2 (de)
JP (1)	JP4121160B2 (de)
KR (1)	KR100314969B1 (de)
CN (1)	CN1053468C (de)
AT (1)	ATE187865T1 (de)
AU (1)	AU675094B2 (de)
CA (1)	CA2113374C (de)
DE (1)	DE69422205T3 (de)
DK (1)	DK0608716T4 (de)
ES (1)	ES2140471T5 (de)
GR (1)	GR3032584T3 (de)
IL (1)	IL108330A (de)
MA (1)	MA23089A1 (de)
PT (1)	PT608716E (de)
TR (1)	TR28634A (de)
TW (1)	TW367365B (de)
ZA (1)	ZA94289B (de)

Families Citing this family (24)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US6164973A (en) *	1995-01-20	2000-12-26	Vincent J. Macri	Processing system method to provide users with user controllable image for use in interactive simulated physical movements
GB9703628D0 (en) *	1997-02-21	1997-04-09	Abdelrahman Layla Z	Sugar cane production
AR019320A1 (es) *	1998-06-12	2002-02-13	Silvagen Inc	Metodo de siembra y germinacion ex vitro de embriones somaticos de plantas
US6946295B2 (en)	1998-06-12	2005-09-20	Cellfor, Inc.	Process for ex vitro sowing and germination of plant somatic embryos
US6265217B1 (en) *	2000-03-01	2001-07-24	Tong Yang Moolsan Company Limited	Method for producing microbulbs of garlic {Allium sativum l.} in vitro
US7598073B2 (en)	2002-04-09	2009-10-06	Weyerhaeuser Nr Company	Methods for producing high yields of zygotic-like cotyledonary pine embryos utilizing media that include a disaccharide and glucose
US7381562B2 (en)	2002-05-30	2008-06-03	Weyerhaeuser Company	Methods for producing cotyledonary pine embryos utilizing a gibberellin
CA2435337C (en)	2002-11-14	2010-03-23	Weyerhaeuser Company	Methods for producing conifer somatic embryos
US7521237B2 (en)	2003-06-23	2009-04-21	Weyerhaeuser Nr Company	Methods for promoting maturation of conifer somatic embryos
US20040266002A1 (en) *	2003-06-30	2004-12-30	Weyerhaeuser Company	Use of abscisic acid in somatic embryogenesis of pine trees
US7732205B2 (en)	2003-07-30	2010-06-08	Weyerhaeuser Nr Company	Development and stratification of pine somatic embryos using a liquid system
US7520089B2 (en)	2003-11-25	2009-04-21	Weyerhaeuser Nr Company	Method to improve manufactured seed germination
CA2495650C (en)	2004-02-25	2011-05-24	Weyerhaeuser Company	Continuous culture of conifer embryogenic tissue
US7452722B2 (en)	2004-03-02	2008-11-18	Weyerhaeuser Company	Methods for developing conifer somatic embryos
CA2568476C (en)	2005-12-22	2015-03-17	Weyerhaeuser Company	Methods for storing conifer somatic embryo germinants
US7858371B2 (en) *	2007-09-26	2010-12-28	Weyerhaeuser Nr Company	Method of separating embryo suspension mass
EP2132978A1 (de) *	2008-06-13	2009-12-16	Syngeta Participations AG	Verfahren für die Herstellung somatischer Embryonen aus Pflanzengewebe von Euphorbia pulcherrima
CN102763598B (zh) *	2012-08-16	2013-09-11	云南省农业科学院花卉研究所	利用体细胞胚繁育文山兜兰苗的方法
CN105104183B (zh) *	2015-08-26	2017-07-04	北京农业生物技术研究中心	一种菊苣原生质体诱导纯合四倍体植物的方法
US10043133B2 (en) *	2016-04-08	2018-08-07	Pearson Education, Inc.	Systems and methods of event-based content provisioning
CN107164303B (zh) *	2017-07-19	2020-11-20	泗县微腾知识产权运营有限公司	一种南瓜游离小孢子诱导培养基及诱导培养方法
ES2914629B2 (es) *	2020-12-14	2023-03-06	Instituto Madrileno De Investig Y Desarrollo Rural Agrario Y Alimentario Imidra	Procedimiento para la obtencion de biomasa y/o sustancias de valor utilizables como aditivos para piensos a partir de cultivos embriogenicos de encina o alcornoque y producto obtenido.
CN114847159B (zh) *	2022-04-28	2023-04-07	云南龙藏生物科技有限公司	一种川贝母鳞茎组织培养再生方法
PY2423542A (es)	2023-03-31	2024-12-06	CTC Centro de Tecnologia Canavieira S A	Proceso de cultivo de agregados celulares, agregados celulares, método de micropropagación de células vegetales y método de maduración de embriones somáticos

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US5024944A (en) *	1986-08-04	1991-06-18	Lubrizol Genetics, Inc.	Transformation, somatic embryogenesis and whole plant regeneration method for Glycine species
US5030572A (en) *	1987-04-01	1991-07-09	Lubrizol Genetics, Inc.	Sunflower regeneration from cotyledons
ES2043884T3 (es) *	1987-11-18	1994-01-01	Ciba Geigy Ag	Metodo de gran rendimiento para la preparacion de algodon a partir de celulas cultivadas.
EP0328424A3 (de) *	1988-02-12	1992-04-29	Kyowa Hakko Kogyo Kabushiki Kaisha	Verfahren zur Produktion von somatischen Embryos
US5236841A (en) *	1989-03-09	1993-08-17	Gupta Pramod K	Method for reproducing conifers by somatic embryogenesis using stepwise hormone adjustment
US5482857A (en) *	1989-03-09	1996-01-09	Weyerhaeuser Company	Method for reproducing douglas-fir by somatic embryogenesis
US4957866A (en) *	1989-03-09	1990-09-18	Weyerhaeuser Company	Method for reproducing coniferous plants by somatic embryogenesis
EP0446374B1 (de) *	1989-09-30	1996-02-07	Kirin Beer Kabushiki Kaisha	Verfahren zur produktion von sämlingen
DK0517833T4 (da) *	1990-03-02	2008-11-17	Bayer Bioscience Nv	Genetisk transformation og regenering af sukkerroer
US5334530A (en) *	1992-03-10	1994-08-02	Woods Susan H	Method and media for the somatic embryogenesis and regeneration of bamboo
WO1993023529A2 (fr) *	1992-05-12	1993-11-25	Champagne Moët & Chandon	Culture stabilisee d'agregats cellulaires et procede de developpement des embryons a partir d'une souche proembryogene destine a la regeneration de la vigne
US5294594A (en) *	1992-12-23	1994-03-15	American Cyanamid Company	Water dispersible granular herbicidal compositions comprising dinitroaniline herbicides, montmorillonite carrier and water-swellable polymer
US5491090A (en) *	1994-02-09	1996-02-13	Westvaco Corporation	Embryogenic coniferous liquid suspension cultures

1994
- 1994-01-13 EP EP94100442A patent/EP0608716B2/de not_active Expired - Lifetime
- 1994-01-13 AU AU53142/94A patent/AU675094B2/en not_active Ceased
- 1994-01-13 DE DE69422205T patent/DE69422205T3/de not_active Expired - Lifetime
- 1994-01-13 DK DK94100442T patent/DK0608716T4/da active
- 1994-01-13 IL IL10833094A patent/IL108330A/en not_active IP Right Cessation
- 1994-01-13 CA CA002113374A patent/CA2113374C/en not_active Expired - Lifetime
- 1994-01-13 TR TR00024/94A patent/TR28634A/xx unknown
- 1994-01-13 ES ES94100442T patent/ES2140471T5/es not_active Expired - Lifetime
- 1994-01-13 PT PT94100442T patent/PT608716E/pt unknown
- 1994-01-13 AT AT94100442T patent/ATE187865T1/de active
- 1994-01-13 EP EP99108585A patent/EP0934691A3/de not_active Withdrawn
- 1994-01-14 CN CN94100613A patent/CN1053468C/zh not_active Expired - Lifetime
- 1994-01-14 JP JP00247094A patent/JP4121160B2/ja not_active Expired - Lifetime
- 1994-01-14 MA MA23395A patent/MA23089A1/fr unknown
- 1994-01-14 ZA ZA94289A patent/ZA94289B/xx unknown
- 1994-01-14 KR KR1019940000563A patent/KR100314969B1/ko not_active Expired - Fee Related
- 1994-01-18 TW TW083100355A patent/TW367365B/zh not_active IP Right Cessation
1995
- 1995-03-20 US US08/406,822 patent/US5587312A/en not_active Expired - Lifetime
2000
- 2000-02-04 GR GR20000400279T patent/GR3032584T3/el unknown

Also Published As

Publication number	Publication date
PT608716E (pt)	2000-05-31
GR3032584T3 (en)	2000-05-31
CA2113374A1 (en)	1994-07-16
TR28634A (tr)	1996-12-04
JPH06237659A (ja)	1994-08-30
DK0608716T3 (da)	2000-06-13
ATE187865T1 (de)	2000-01-15
AU675094B2 (en)	1997-01-23
IL108330A0 (en)	1994-04-12
DK0608716T4 (da)	2003-11-24
DE69422205D1 (de)	2000-01-27
EP0934691A3 (de)	2001-03-28
KR100314969B1 (ko)	2002-02-19
ES2140471T3 (es)	2000-03-01
AU5314294A (en)	1994-07-21
EP0608716B2 (de)	2003-08-06
MA23089A1 (fr)	1994-01-10
CN1053468C (zh)	2000-06-14
EP0934691A2 (de)	1999-08-11
DE69422205T3 (de)	2004-04-29
EP0608716A3 (de)	1994-12-07
TW367365B (en)	1999-08-21
ZA94289B (en)	1995-07-14
EP0608716A2 (de)	1994-08-03
EP0608716B1 (de)	1999-12-22
US5587312A (en)	1996-12-24
KR940018012A (ko)	1994-08-16
ES2140471T5 (es)	2004-06-01
JP4121160B2 (ja)	2008-07-23
CN1092107A (zh)	1994-09-14
DE69422205T2 (de)	2000-09-07
CA2113374C (en)	2008-04-29

Legal Events

Date	Code	Title
2004-07-25	FF	Patent granted
2008-02-10	KB	Patent renewed
2012-01-31	KB	Patent renewed
2014-09-30	EXP	Patent expired

Publication	Publication Date	Title
EP0608716B2 (de)	2003-08-06	Verbesserungen in der somatischen Embryogenese
Klimaszewska et al.	2002	Conifer somatic embryogenesis: I. Development
Winkelmann et al.	1998	Establishing embryogenie suspension cultures in Cyclamen persicum'Purple Flamed'
Ara et al.	2000	Plant regeneration from protoplasts of mango (Mangifera indica L.) through somatic embryogenesis
US20090111182A1 (en)	2009-04-30	Method for Propagation of Plant
Tang et al.	2001	Plant regeneration from embryogenic cultures initiated from mature loblolly pine zygotic embryos
Lam	1977	Regeneration of plantlets from single cells in potatoes
US7785884B2 (en)	2010-08-31	Low density spreading methods for conifer somatic embryogenesis
US7732205B2 (en)	2010-06-08	Development and stratification of pine somatic embryos using a liquid system
Wang et al.	2001	Plant regeneration from embryogenic suspension cultures of dune reed
US7452722B2 (en)	2008-11-18	Methods for developing conifer somatic embryos
CA2604700C (en)	2012-12-11	Low density spreading methods for somatic embryogenesis
US7888099B2 (en)	2011-02-15	Methods for producing a synchronized population of conifer somatic embryos
EP1491089A1 (de)	2004-12-29	Medien und Methoden für die Förderung der Entwicklung von somatischen Embryonen für Nadelbäume
Kanwar et al.	2008	Plant regeneration in Robinia pseudoacacia from cell suspension cultures
Newton et al.	2005	Slash pine (Pinus elliottii Engelm.)
Denchev et al.	1995	Micropropagation through somatic embryos
Ruffoni et al.	2006	Somatic embryogenesis in floricultural crops: experiences of massive propagation of Lisianthus, Genista and Cyclamen
AU2003231584B2 (en)	2007-09-20	Methods for producing conifer somatic embryos
Kanchanapoom et al.	1999	Cell suspension, isolation and culture of cacao (Theobroma cacao L.) protoplasts
DENCHEV et al.	2013	II. 6 Micropropagation Through Somatic Embryos
US20040237130A1 (en)	2004-11-25	Embryogenic culture initiation of douglas-fir by maltose
CA2470448A1 (en)	2004-12-30	Use of a abscisic acid in somatic embryogenesis of pine trees