IL155138A - Gel trap for electrophoresis - Google Patents

Gel trap for electrophoresis

Info

Publication number: IL155138A
Authority: IL; Israel
Prior art keywords: gel; chamber; matrix; gel matrix; aaid
Prior art date: 2000-11-02

Application number

IL155138A

Other languages

English (en)

Original Assignee

Gene Bio Applic Ltd

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

2000-11-02

Filing date

2003-03-27

Publication date

2007-06-03

2000-11-02 Priority claimed from IL13944600A external-priority patent/IL139446A0/xx

2000-11-02 Priority claimed from IL13944700A external-priority patent/IL139447A0/xx

2003-03-27 Application filed by Gene Bio Applic Ltd filed Critical Gene Bio Applic Ltd

2003-03-27 Priority to IL155138A priority Critical patent/IL155138A/en

2007-06-03 Publication of IL155138A publication Critical patent/IL155138A/en

Links

238000001962 electrophoresis Methods 0.000 title claims abstract description 42
238000000034 method Methods 0.000 claims abstract description 40
239000011159 matrix material Substances 0.000 claims description 124
238000004891 communication Methods 0.000 claims description 54
238000010521 absorption reaction Methods 0.000 claims description 35
150000001875 compounds Chemical class 0.000 claims description 30
239000000463 material Substances 0.000 claims description 30
239000013076 target substance Substances 0.000 claims description 30
229920000936 Agarose Polymers 0.000 claims description 18
238000004043 dyeing Methods 0.000 claims description 18
239000007853 buffer solution Substances 0.000 claims description 13
239000000872 buffer Substances 0.000 claims description 11
239000000853 adhesive Substances 0.000 claims description 4
230000001070 adhesive effect Effects 0.000 claims description 4
238000007789 sealing Methods 0.000 claims description 2
239000000499 gel Substances 0.000 description 186
OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 17
239000011543 agarose gel Substances 0.000 description 12
239000000975 dye Substances 0.000 description 9
239000000243 solution Substances 0.000 description 9
239000000126 substance Substances 0.000 description 9
239000000203 mixture Substances 0.000 description 8
238000005342 ion exchange Methods 0.000 description 7
238000013508 migration Methods 0.000 description 6
230000005012 migration Effects 0.000 description 6
238000005266 casting Methods 0.000 description 5
231100000331 toxic Toxicity 0.000 description 5
230000002588 toxic effect Effects 0.000 description 5
HRPVXLWXLXDGHG-UHFFFAOYSA-N Acrylamide Chemical compound NC(=O)C=C HRPVXLWXLXDGHG-UHFFFAOYSA-N 0.000 description 4
ZMMJGEGLRURXTF-UHFFFAOYSA-N ethidium bromide Chemical compound [Br-].C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CC)=C1C1=CC=CC=C1 ZMMJGEGLRURXTF-UHFFFAOYSA-N 0.000 description 4
229960005542 ethidium bromide Drugs 0.000 description 4
238000001502 gel electrophoresis Methods 0.000 description 4
102000004169 proteins and genes Human genes 0.000 description 4
108090000623 proteins and genes Proteins 0.000 description 4
230000002745 absorbent Effects 0.000 description 3
239000002250 absorbent Substances 0.000 description 3
238000010276 construction Methods 0.000 description 3
239000000356 contaminant Substances 0.000 description 3
230000005684 electric field Effects 0.000 description 3
238000002372 labelling Methods 0.000 description 3
231100000614 poison Toxicity 0.000 description 3
241000894007 species Species 0.000 description 3
239000003440 toxic substance Substances 0.000 description 3
VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 2
230000000711 cancerogenic effect Effects 0.000 description 2
231100000315 carcinogenic Toxicity 0.000 description 2
238000013461 design Methods 0.000 description 2
239000008151 electrolyte solution Substances 0.000 description 2
239000007850 fluorescent dye Substances 0.000 description 2
239000002245 particle Substances 0.000 description 2
238000000926 separation method Methods 0.000 description 2
239000000741 silica gel Substances 0.000 description 2
229910002027 silica gel Inorganic materials 0.000 description 2
230000002123 temporal effect Effects 0.000 description 2
238000012800 visualization Methods 0.000 description 2
229920001817 Agar Polymers 0.000 description 1
238000000636 Northern blotting Methods 0.000 description 1
239000004793 Polystyrene Substances 0.000 description 1
CGNLCCVKSWNSDG-UHFFFAOYSA-N SYBR Green I Chemical compound CN(C)CCCN(CCC)C1=CC(C=C2N(C3=CC=CC=C3S2)C)=C2C=CC=CC2=[N+]1C1=CC=CC=C1 CGNLCCVKSWNSDG-UHFFFAOYSA-N 0.000 description 1
DZBUGLKDJFMEHC-UHFFFAOYSA-N acridine Chemical compound C1=CC=CC2=CC3=CC=CC=C3N=C21 DZBUGLKDJFMEHC-UHFFFAOYSA-N 0.000 description 1
239000008186 active pharmaceutical agent Substances 0.000 description 1
239000008272 agar Substances 0.000 description 1
150000001408 amides Chemical class 0.000 description 1
239000000427 antigen Substances 0.000 description 1
102000036639 antigens Human genes 0.000 description 1
108091007433 antigens Proteins 0.000 description 1
229910052799 carbon Inorganic materials 0.000 description 1
150000001768 cations Chemical class 0.000 description 1
238000004140 cleaning Methods 0.000 description 1
238000011109 contamination Methods 0.000 description 1
230000001419 dependent effect Effects 0.000 description 1
230000001627 detrimental effect Effects 0.000 description 1
238000001035 drying Methods 0.000 description 1
230000009881 electrostatic interaction Effects 0.000 description 1
238000002474 experimental method Methods 0.000 description 1
238000003018 immunoassay Methods 0.000 description 1
239000007788 liquid Substances 0.000 description 1
229920002521 macromolecule Polymers 0.000 description 1
239000012528 membrane Substances 0.000 description 1
238000004806 packaging method and process Methods 0.000 description 1
230000002093 peripheral effect Effects 0.000 description 1
229920003023 plastic Polymers 0.000 description 1
239000004033 plastic Substances 0.000 description 1
229920002401 polyacrylamide Polymers 0.000 description 1
229920002223 polystyrene Polymers 0.000 description 1
238000012545 processing Methods 0.000 description 1
-1 proteins Chemical class 0.000 description 1
238000011160 research Methods 0.000 description 1
239000012146 running buffer Substances 0.000 description 1
238000001612 separation test Methods 0.000 description 1
150000003384 small molecules Chemical class 0.000 description 1
238000000638 solvent extraction Methods 0.000 description 1
238000010186 staining Methods 0.000 description 1
230000001954 sterilising effect Effects 0.000 description 1
238000012546 transfer Methods 0.000 description 1
239000012780 transparent material Substances 0.000 description 1
238000011144 upstream manufacturing Methods 0.000 description 1
238000001262 western blot Methods 0.000 description 1
238000007693 zone electrophoresis Methods 0.000 description 1

Classifications

- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44704—Details; Accessories
- G01N27/44743—Introducing samples
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/24—Extraction; Separation; Purification by electrochemical means
- C07K1/26—Electrophoresis
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44704—Details; Accessories
- G01N27/44747—Composition of gel or of carrier mixture

Landscapes

Chemical & Material Sciences (AREA)
Health & Medical Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Molecular Biology (AREA)
Chemical Kinetics & Catalysis (AREA)
Electrochemistry (AREA)
Biochemistry (AREA)
General Health & Medical Sciences (AREA)
Analytical Chemistry (AREA)
Immunology (AREA)
Physics & Mathematics (AREA)
General Physics & Mathematics (AREA)
Organic Chemistry (AREA)
Pathology (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Biophysics (AREA)
Genetics & Genomics (AREA)
Medicinal Chemistry (AREA)
General Chemical & Material Sciences (AREA)
Dispersion Chemistry (AREA)
Investigating Or Analysing Biological Materials (AREA)
Apparatus Associated With Microorganisms And Enzymes (AREA)
Electrostatic Separation (AREA)
Peptides Or Proteins (AREA)
Polysaccharides And Polysaccharide Derivatives (AREA)
Physical Or Chemical Processes And Apparatus (AREA)
Jellies, Jams, And Syrups (AREA)

IL155138A 2000-11-02 2003-03-27 Gel trap for electrophoresis IL155138A (en)

Priority Applications (1)

Application Number	Priority Date	Filing Date	Title
IL155138A IL155138A (en)	2000-11-02	2003-03-27	Gel trap for electrophoresis

Applications Claiming Priority (4)

Application Number	Priority Date	Filing Date	Title
IL13944600A IL139446A0 (en)	2000-11-02	2000-11-02	Gel for electrophoresis
IL13944700A IL139447A0 (en)	2000-11-02	2000-11-02	Gel trap for electrophoresis
PCT/IL2001/001000 WO2002037093A2 (fr)	2000-11-02	2001-10-29	Piege en gel pour electrophorese
IL155138A IL155138A (en)	2000-11-02	2003-03-27	Gel trap for electrophoresis

Publications (1)

Publication Number	Publication Date
IL155138A true IL155138A (en)	2007-06-03

Family

ID=26323988

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
IL155138A IL155138A (en)	2000-11-02	2003-03-27	Gel trap for electrophoresis

Country Status (9)

Country	Link
US (3)	US20040020775A1 (fr)
EP (2)	EP1330643B1 (fr)
AT (1)	ATE372514T1 (fr)
AU (4)	AU2002212670B2 (fr)
CA (2)	CA2427022A1 (fr)
DE (1)	DE60130353T2 (fr)
ES (1)	ES2292633T3 (fr)
IL (1)	IL155138A (fr)
WO (2)	WO2002037093A2 (fr)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
KR100351831B1 (ko) *	2000-10-02	2002-09-11	엘지전자 주식회사	Ｖｓｂ 송신 시스템
IL166716A0 (en) *	2005-02-07	2006-01-15	Gene Bio Applic Ltd	Double chamber tank for electrophoresis
USD563562S1 (en) *	2005-02-15	2008-03-04	Serenex, Inc.	Gel box for an auto sample feed gel system
HK1206430A1 (en) *	2012-05-31	2016-01-08	Ge Healthcare Bio-Sciences Ab	An electrophoresis system and a separation and identification method
CN104364640B (zh) *	2012-05-31	2016-10-26	通用电气健康护理生物科学股份公司	制造电泳盒的方法
WO2014150275A1 (fr) *	2013-03-15	2014-09-25	Bio-Rad Laboratories, Inc.	Gels de polyacrylamide stables au stockage pour coulée, transfert et prise d'image rapides
WO2015106356A1 (fr) *	2014-01-17	2015-07-23	Coastal Genomics Inc.	Cassettes à utiliser lors d'analyses électrophorétiques parallèles automatisées et leurs procédés de fabrication et d'utilisation
WO2016187535A2 (fr) *	2015-05-20	2016-11-24	Zephyrus Biosciences, Inc.	Systèmes et procédés pour séparation électrophorétique et analyse de substances à analyser
WO2021030427A2 (fr) *	2019-08-12	2021-02-18	Yale University	Système de chargement d'échantillon dans un puits intragel
EP4419901A4 (fr) *	2021-10-21	2025-11-19	Zadeh Cameron O	Appareil de gel électrophorétique à multiplexage à haut débit et procédés associés

Family Cites Families (23)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
NL151506B (nl) *	1971-08-12	1976-11-15	Biotest Serum Institut Gmbh	Werkwijze en inrichting voor het uitvoeren van een tweedimensionale immuunelektroforese.
AT320865B (de) *	1973-02-12	1975-03-10	Immuno Ag	Verfahren zum Bestimmen von normalen und pathologischen Plasma-Lipoproteinen in menschlichen Körperflüssigkeiten und Apparat zur Durchführung des Verfahrens
US3888759A (en) *	1973-05-25	1975-06-10	Yeda Res & Dev	Flat plate electrophoresis
DE2406959C3 (de) *	1974-02-14	1979-02-08	Behringwerke Ag, 3550 Marburg	Anordnung und Verfahren zur Bestimmung von Antigenen
DE3232685C2 (de) *	1982-09-02	1985-02-14	Reiner Dr. 8039 Puchheim Westermeier	Gelmatrix zur horizontalen ein- und/oder zweidimensionalen elektrophoretischen Auftrennung von Anionen und Kationen, Verfahren zu deren Herstellung und Verwendung eines Spritzensystems zur Probenaufgabe für die Gelmatrix
FR2533704B1 (fr) *	1982-09-27	1986-03-21	Spiral	Dosage immunologique dans le serum de l'apolipoproteine b des lipoproteines de basse densite
US4666581A (en) *	1984-05-09	1987-05-19	Hitachi, Ltd.	Apparatus for two-dimensional electrophoresis
US4608146A (en) *	1985-04-09	1986-08-26	Bio-Rad Laboratories, Inc.	Horizontal electrophoresis cell for rapid assembly
US4735697A (en) *	1985-04-17	1988-04-05	Phoresis Transfer Systems, Inc.	Method and apparatus for separating complex mixtures of bio-organic materials
US4709810A (en) *	1986-11-14	1987-12-01	Helena Laboratories Corporation	Container for an electrophoretic support medium
US4830725A (en) *	1987-08-04	1989-05-16	Life Technologies, Inc.	Electrophoresis apparatus
US5085756A (en) *	1990-08-21	1992-02-04	Hewlett-Packard Company	Column separation system for electrophoresis with sample pretreatment
US5228971A (en) *	1990-12-20	1993-07-20	Wisconsin Alumni Research Foundation	Horizontal gel electrophoresis apparatus
CA2083820A1 (fr) *	1991-03-28	1992-09-29	Noubar B. Afeyan	Identification par soustraction d'un produit contenu dans un debit chromatographique
US5443704A (en) *	1991-12-31	1995-08-22	Fmc Corporation	Electrophoresis gel container assemblies
US6129828A (en) *	1996-09-06	2000-10-10	Nanogen, Inc.	Apparatus and methods for active biological sample preparation
WO1995014921A1 (fr) *	1993-11-23	1995-06-01	Perkin-Elmer Corporation	Piegeage de matrices de sequençage d'acide nucleique dans des melanges d'echantillonnage par des reseaux polymeres enchevetres
IL112147A (en) *	1994-01-19	1999-12-22	Du Pont	Sample holder and method for automated electrophoresis
US5582702A (en) *	1995-04-26	1996-12-10	Ethrog Biotechnology Ltd.	Apparatus and method for electrophoresis
US5827418A (en) *	1996-10-11	1998-10-27	Hoefer Pharmacia Biotech, Inc.	Electrophoresis cassette
US5800691A (en) *	1996-12-23	1998-09-01	Guest Elchrom Scientific Ag	Electrophoresis gels containing sample wells with enlarged loading area
AU1804099A (en) *	1997-12-05	1999-06-28	Mosaic Technologies	Slotted electrophoresis gel composition and methods of use thereof
DE19830988A1 (de) *	1998-07-10	2000-01-20	Lion Bioscience Ag	Verfahren zur Zuführung von Probenmaterial, Probenzuführteil sowie Elektrophoresevorrichtung

2001
- 2001-10-29 WO PCT/IL2001/001000 patent/WO2002037093A2/fr not_active Ceased
- 2001-10-29 AU AU2002212670A patent/AU2002212670B2/en not_active Ceased
- 2001-10-29 EP EP01980886A patent/EP1330643B1/fr not_active Expired - Lifetime
- 2001-10-29 AU AU2002214219A patent/AU2002214219B2/en not_active Ceased
- 2001-10-29 AU AU1267002A patent/AU1267002A/xx active Pending
- 2001-10-29 AU AU1421902A patent/AU1421902A/xx active Pending
- 2001-10-29 EP EP01982678A patent/EP1330644A2/fr not_active Withdrawn
- 2001-10-29 WO PCT/IL2001/001001 patent/WO2002037094A2/fr not_active Ceased
- 2001-10-29 AT AT01980886T patent/ATE372514T1/de not_active IP Right Cessation
- 2001-10-29 DE DE60130353T patent/DE60130353T2/de not_active Expired - Lifetime
- 2001-10-29 ES ES01980886T patent/ES2292633T3/es not_active Expired - Lifetime
- 2001-10-29 CA CA002427022A patent/CA2427022A1/fr not_active Abandoned
- 2001-10-29 CA CA2427025A patent/CA2427025C/fr not_active Expired - Fee Related
2003
- 2003-03-27 IL IL155138A patent/IL155138A/en not_active IP Right Cessation
- 2003-04-23 US US10/422,061 patent/US20040020775A1/en not_active Abandoned
- 2003-04-23 US US10/422,062 patent/US7264703B2/en not_active Expired - Fee Related
2006
- 2006-03-08 US US11/371,313 patent/US7320747B2/en not_active Expired - Fee Related

Also Published As

Publication number	Publication date
CA2427025C (fr)	2011-09-20
WO2002037093A3 (fr)	2002-07-25
AU2002212670B2 (en)	2006-08-17
US7320747B2 (en)	2008-01-22
ATE372514T1 (de)	2007-09-15
DE60130353D1 (de)	2007-10-18
US20040020776A1 (en)	2004-02-05
CA2427022A1 (fr)	2002-05-10
AU1421902A (en)	2002-05-15
WO2002037094A2 (fr)	2002-05-10
AU2002214219B2 (en)	2006-08-17
EP1330643B1 (fr)	2007-09-05
DE60130353T2 (de)	2008-05-29
ES2292633T3 (es)	2008-03-16
US20040020775A1 (en)	2004-02-05
WO2002037094A3 (fr)	2002-07-25
EP1330644A2 (fr)	2003-07-30
AU1267002A (en)	2002-05-15
CA2427025A1 (fr)	2002-05-10
EP1330643A2 (fr)	2003-07-30
US7264703B2 (en)	2007-09-04
WO2002037093A2 (fr)	2002-05-10
US20060207882A1 (en)	2006-09-21

Legal Events

Date	Code	Title	Description
2012-10-31	MM9K	Patent not in force due to non-payment of renewal fees

Publication	Publication Date	Title
US8361299B2 (en)	2013-01-29	Multichannel preparative electrophoresis system
US7264703B2 (en)	2007-09-04	Gel trap for electrophoresis
US6602391B2 (en)	2003-08-05	Apparatus and method for combined capillary separation and blotting of biological macromolecules
EP0749576A1 (fr)	1996-12-27	Procedes de preparation de gels de polyacrylamide pour analyse electrophoretique
US8449745B2 (en)	2013-05-28	Monolithic electrophoresis flat gel system
JP2004510168A (ja)	2004-04-02	電気泳動システム
AU2002214219A1 (en)	2002-07-18	Gel trap for electrophoresis
US4391688A (en)	1983-07-05	Electrophoresis system for multiple agarose slab gels
EP2986978B1 (fr)	2019-05-15	Peigne pour mini-gel
AU2002212670A1 (en)	2002-07-18	Gel for electrophoresis
EP0871870A1 (fr)	1998-10-21	Element de chargement a membrane pour electrophorese sur gel
US4750506A (en)	1988-06-14	Apparatus for processing a slab gel
CA2424298A1 (fr)	2003-04-04	Procede et dispositif pour electrophorese en 2d dans des gels importants
Lapin et al.	1985	A simple routine method for SDS-electrophoresis of urinary proteins in kidney transplant patients
CA1181034A (fr)	1985-01-15	Systeme d'electrophorese pour plaques de gel d'agarose multiples
US5747600A (en)	1998-05-05	Reconstitutable polyacrylamide materials and methods for producing same
CA2429266A1 (fr)	2002-05-30	Passerelles isoelectriques et procede et appareil permettant d'utiliser lesdites passerelles
JPS61105453A (ja)	1986-05-23	多数アガロ−ススラブゲルの連続電気泳動装置
WO2022023959A1 (fr)	2022-02-03	Dispositifs, systèmes et procédés d'électrotransfert et d'électrophorèse
WO1997019989A1 (fr)	1997-06-05	Materiaux a base de polyacrylamide pouvant etre reconstitues et procedes de production
WO2003070360A1 (fr)	2003-08-28	Dispositif de separation de petits volumes
Nowotny	1979	Separation of Human Hemoglobins by Electrofocusing