IL166757A0 - A method of isolating cells - Google Patents

A method of isolating cells

Info

Publication number
IL166757A0
IL166757A0 IL16675702A IL16675702A IL166757A0 IL 166757 A0 IL166757 A0 IL 166757A0 IL 16675702 A IL16675702 A IL 16675702A IL 16675702 A IL16675702 A IL 16675702A IL 166757 A0 IL166757 A0 IL 166757A0
Authority
IL
Israel
Prior art keywords
cell lines
well
generating cell
cells
protein
Prior art date
Application number
IL16675702A
Other languages
English (en)
Original Assignee
Chromocell Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Chromocell Corp filed Critical Chromocell Corp
Publication of IL166757A0 publication Critical patent/IL166757A0/xx

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6816Hybridisation assays characterised by the detection means
    • C12Q1/6818Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07HSUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/04Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6809Methods for determination or identification of nucleic acids involving differential detection
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6841In situ hybridisation
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/158Expression markers
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00Screening for compounds of potential therapeutic value
    • G01N2500/04Screening involving studying the effect of compounds C directly on molecule A (e.g. C are potential ligands for a receptor A, or potential substrates for an enzyme A)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
IL16675702A 1999-11-23 2002-08-28 A method of isolating cells IL166757A0 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US16698799P 1999-11-23 1999-11-23
US09/718,448 US6692965B1 (en) 1999-11-23 2000-11-22 Isolation of living cells and preparation of cell lines based on detection and quantification of preselected cellular ribonucleic acid sequences
PCT/US2002/027749 WO2004020625A1 (en) 1999-11-23 2002-08-28 SELECTION AND ISOLATION OF LIVING CELLS USING mRNA-BINDING PROBES

Publications (1)

Publication Number Publication Date
IL166757A0 true IL166757A0 (en) 2006-01-15

Family

ID=36640908

Family Applications (1)

Application Number Title Priority Date Filing Date
IL16675702A IL166757A0 (en) 1999-11-23 2002-08-28 A method of isolating cells

Country Status (15)

Country Link
US (4) US6692965B1 (xx)
EP (2) EP2264165B1 (xx)
JP (1) JP4502808B2 (xx)
KR (1) KR101257500B1 (xx)
CN (1) CN1688693B (xx)
AT (1) ATE541924T1 (xx)
AU (1) AU2002332768A1 (xx)
CA (1) CA2496821C (xx)
DK (2) DK2264165T3 (xx)
ES (2) ES2379731T3 (xx)
IL (1) IL166757A0 (xx)
MX (1) MXPA05002192A (xx)
NZ (1) NZ538852A (xx)
PT (1) PT1546327E (xx)
WO (1) WO2004020625A1 (xx)

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US6692965B1 (en) * 1999-11-23 2004-02-17 Chromocell Corporation Isolation of living cells and preparation of cell lines based on detection and quantification of preselected cellular ribonucleic acid sequences
KR20150005720A (ko) * 2004-02-18 2015-01-14 크로모셀 코포레이션 신호 프로브를 이용하는 방법 및 물질
CA2667631C (en) 2006-10-10 2017-11-07 Miacom Diagnostics Gmbh Nucleic acid beacons for fluorescent in-situ hybridisation and chip technology
CN101611137A (zh) * 2006-11-30 2009-12-23 克罗莫塞尔公司 用于蛋白质生产的最优化的宿主细胞
US20080216185A1 (en) * 2007-01-19 2008-09-04 Invitrogen Corporation Compositions and Methods for Genetic Manipulation and Monitoring of Cell Lines
ES2391906T3 (es) * 2007-09-12 2012-11-30 Institut Pasteur Polinucleótido apto para un ensayo indicador basado en una sola célula para controlar los patrones de expresión génica con una resolución espacio-temporal elevada
US20100297674A1 (en) * 2008-01-22 2010-11-25 Chromocell Corporation NOVEL CELL LINES EXPRESSING NaV AND METHODS USING THEM
US9534035B2 (en) * 2008-01-25 2017-01-03 Chromocell Corporation Cell lines expressing ENaC and methods using them
NZ586957A (en) * 2008-02-01 2014-03-28 Chromocell Corp Cell lines and methods for making and using them
EP2186827A1 (en) 2008-11-14 2010-05-19 HS LifeSciences Ltd. Surrogate marker directed cDNA cloning of selectively induced mRNAs
EP2391711B1 (en) * 2009-02-02 2015-04-08 Chromocell Corporation Novel cell lines and methods
WO2011014740A2 (en) 2009-07-31 2011-02-03 Chromocell Corporation Methods and composition for identifying and validating modulators of cell fate
US20120131701A1 (en) 2009-08-05 2012-05-24 Chromocell Corporation Improved plants, microbes, and organisms
CN103998933B (zh) 2011-10-20 2016-12-14 卓莫赛尔公司 鉴定调节苦味的化合物的测定
CN119655753A (zh) * 2012-12-14 2025-03-21 明德拉公司 用于检测和获取生物标志物的方法和装置
WO2014144991A1 (en) * 2013-03-15 2014-09-18 Chromocell Corporation Methods and materials using signaling probes
US9493742B2 (en) 2013-03-15 2016-11-15 Emory University Purification of cell mixtures using molecular beacons targeting cell specific RNA
WO2014151994A1 (en) 2013-03-15 2014-09-25 Kambiz Shekdar Genome editing using effector oligonucleotides for therapeutic treatment
CN105358983B (zh) 2013-04-24 2018-08-03 卓莫赛尔公司 用于鉴定调节苦味的化合物的测定
US20160177396A1 (en) * 2014-12-22 2016-06-23 Enzo Biochem, Inc. Comprehensive and comparative flow cytometry-based methods for identifying the state of a biological system
EP3612629A1 (en) 2017-04-18 2020-02-26 The Broad Institute, Inc. Compositions for detecting secretion and methods of use
WO2019210268A2 (en) 2018-04-27 2019-10-31 The Broad Institute, Inc. Sequencing-based proteomics
CN110819656B (zh) * 2019-11-11 2021-06-04 中国科学院上海高等研究院 一种基于同步光源的x-射线多色遗传标记探针及其制备方法以及应用
AU2021385427A1 (en) 2020-11-30 2023-06-29 Mindera Corporation Microneedle devices and methods, and skin condition assays

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Also Published As

Publication number Publication date
ATE541924T1 (de) 2012-02-15
HK1079238A1 (en) 2006-03-31
US20150143563A1 (en) 2015-05-21
DK2264165T3 (da) 2013-08-05
CA2496821C (en) 2012-06-19
EP1546327B1 (en) 2012-01-18
ES2423598T3 (es) 2013-09-23
EP1546327A4 (en) 2005-11-09
DK1546327T3 (da) 2012-03-26
US20100212040A1 (en) 2010-08-19
WO2004020625A1 (en) 2004-03-11
US20060147937A1 (en) 2006-07-06
JP4502808B2 (ja) 2010-07-14
KR101257500B1 (ko) 2013-04-23
EP1546327A1 (en) 2005-06-29
JP2005537005A (ja) 2005-12-08
EP2264165B1 (en) 2013-05-01
CA2496821A1 (en) 2004-03-11
CN1688693B (zh) 2010-12-22
EP2264165A3 (en) 2011-02-16
KR20050084810A (ko) 2005-08-29
MXPA05002192A (es) 2005-08-18
PT1546327E (pt) 2012-03-12
CN1688693A (zh) 2005-10-26
US6692965B1 (en) 2004-02-17
AU2002332768A1 (en) 2004-03-19
EP2264165A2 (en) 2010-12-22
ES2379731T3 (es) 2012-05-03
NZ538852A (en) 2006-03-31

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