IL36707A - Process for the preparation of polynucleotide phosphorylase - Google Patents

Process for the preparation of polynucleotide phosphorylase

Info

Publication number: IL36707A
Authority: IL; Israel
Prior art keywords: pnpase; solution; aqueous; approximately; supernatant
Prior art date: 1970-05-04

Application number

IL36707A

Other languages

English (en)

Hebrew (he)

Other versions

IL36707A0 (en

Original Assignee

Merck & Co Inc

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1970-05-04

Filing date

1971-04-26

Publication date

1974-10-22

1971-04-26 Application filed by Merck & Co Inc filed Critical Merck & Co Inc

1971-06-23 Publication of IL36707A0 publication Critical patent/IL36707A0/xx

1974-10-22 Publication of IL36707A publication Critical patent/IL36707A/en

Links

101001066878 Homo sapiens Polyribonucleotide nucleotidyltransferase 1, mitochondrial Proteins 0.000 title claims description 66
102000002681 Polyribonucleotide nucleotidyltransferase Human genes 0.000 title description 58
238000004519 manufacturing process Methods 0.000 title description 3
239000000243 solution Substances 0.000 claims description 61
239000006228 supernatant Substances 0.000 claims description 25
LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 18
239000000872 buffer Substances 0.000 claims description 16
238000000034 method Methods 0.000 claims description 16
102000004169 proteins and genes Human genes 0.000 claims description 16
108090000623 proteins and genes Proteins 0.000 claims description 16
FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 14
239000000203 mixture Substances 0.000 claims description 14
239000000463 material Substances 0.000 claims description 13
BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims description 10
OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims description 9
229910052921 ammonium sulfate Inorganic materials 0.000 claims description 9
235000011130 ammonium sulphate Nutrition 0.000 claims description 9
239000007864 aqueous solution Substances 0.000 claims description 9
238000005119 centrifugation Methods 0.000 claims description 9
239000000725 suspension Substances 0.000 claims description 9
230000008569 process Effects 0.000 claims description 7
KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 claims description 6
238000000265 homogenisation Methods 0.000 claims description 2
102100034410 Polyribonucleotide nucleotidyltransferase 1, mitochondrial Human genes 0.000 claims 8
241000025769 Mucor luteus Species 0.000 claims 1
102000009097 Phosphorylases Human genes 0.000 claims 1
108010073135 Phosphorylases Proteins 0.000 claims 1
230000009089 cytolysis Effects 0.000 claims 1
150000003839 salts Chemical class 0.000 claims 1
239000003054 catalyst Substances 0.000 description 24
238000006116 polymerization reaction Methods 0.000 description 18
239000002002 slurry Substances 0.000 description 18
239000001913 cellulose Substances 0.000 description 17
229920002678 cellulose Polymers 0.000 description 17
JPXZQMKKFWMMGK-KQYNXXCUSA-N IDP Chemical compound O[C@@H]1[C@H](O)[C@@H](COP(O)(=O)OP(O)(O)=O)O[C@H]1N1C(N=CNC2=O)=C2N=C1 JPXZQMKKFWMMGK-KQYNXXCUSA-N 0.000 description 16
102000040430 polynucleotide Human genes 0.000 description 16
108091033319 polynucleotide Proteins 0.000 description 16
102000004190 Enzymes Human genes 0.000 description 15
108090000790 Enzymes Proteins 0.000 description 15
229940088598 enzyme Drugs 0.000 description 15
239000002777 nucleoside Substances 0.000 description 14
-1 nucleoside diphosphate Chemical class 0.000 description 14
239000002157 polynucleotide Substances 0.000 description 14
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 13
230000002255 enzymatic effect Effects 0.000 description 12
239000001177 diphosphate Substances 0.000 description 11
235000011180 diphosphates Nutrition 0.000 description 11
230000000694 effects Effects 0.000 description 10
ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 9
239000002253 acid Substances 0.000 description 9
239000011780 sodium chloride Substances 0.000 description 8
239000000758 substrate Substances 0.000 description 8
241000191938 Micrococcus luteus Species 0.000 description 6
HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 6
238000003756 stirring Methods 0.000 description 6
AUHDWARTFSKSAC-HEIFUQTGSA-N (2S,3R,4S,5R)-3,4-dihydroxy-5-(hydroxymethyl)-2-(6-oxo-1H-purin-9-yl)oxolane-2-carboxylic acid Chemical compound [C@]1([C@H](O)[C@H](O)[C@@H](CO)O1)(N1C=NC=2C(O)=NC=NC12)C(=O)O AUHDWARTFSKSAC-HEIFUQTGSA-N 0.000 description 5
239000007853 buffer solution Substances 0.000 description 5
239000012153 distilled water Substances 0.000 description 5
229940028843 inosinic acid Drugs 0.000 description 5
239000004245 inosinic acid Substances 0.000 description 5
239000003960 organic solvent Substances 0.000 description 5
IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 4
RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 4
239000008346 aqueous phase Substances 0.000 description 4
238000000502 dialysis Methods 0.000 description 4
UEGPKNKPLBYCNK-UHFFFAOYSA-L magnesium acetate Chemical compound [Mg+2].CC([O-])=O.CC([O-])=O UEGPKNKPLBYCNK-UHFFFAOYSA-L 0.000 description 4
239000011654 magnesium acetate Substances 0.000 description 4
235000011285 magnesium acetate Nutrition 0.000 description 4
229940069446 magnesium acetate Drugs 0.000 description 4
239000002609 medium Substances 0.000 description 4
239000001509 sodium citrate Substances 0.000 description 4
NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 4
ZWIADYZPOWUWEW-XVFCMESISA-N CDP Chemical compound O=C1N=C(N)C=CN1[C@H]1[C@H](O)[C@H](O)[C@@H](COP(O)(=O)OP(O)(O)=O)O1 ZWIADYZPOWUWEW-XVFCMESISA-N 0.000 description 3
102000004163 DNA-directed RNA polymerases Human genes 0.000 description 3
108090000626 DNA-directed RNA polymerases Proteins 0.000 description 3
UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 3
GRSZFWQUAKGDAV-UHFFFAOYSA-N Inosinic acid Natural products OC1C(O)C(COP(O)(O)=O)OC1N1C(NC=NC2=O)=C2N=C1 GRSZFWQUAKGDAV-UHFFFAOYSA-N 0.000 description 3
102000016943 Muramidase Human genes 0.000 description 3
108010014251 Muramidase Proteins 0.000 description 3
108010062010 N-Acetylmuramoyl-L-alanine Amidase Proteins 0.000 description 3
239000012736 aqueous medium Substances 0.000 description 3
IERHLVCPSMICTF-XVFCMESISA-N cytidine 5'-monophosphate Chemical compound O=C1N=C(N)C=CN1[C@H]1[C@H](O)[C@H](O)[C@@H](COP(O)(O)=O)O1 IERHLVCPSMICTF-XVFCMESISA-N 0.000 description 3
239000008367 deionised water Substances 0.000 description 3
229910021641 deionized water Inorganic materials 0.000 description 3
239000000835 fiber Substances 0.000 description 3
230000002401 inhibitory effect Effects 0.000 description 3
235000013902 inosinic acid Nutrition 0.000 description 3
229960000274 lysozyme Drugs 0.000 description 3
239000004325 lysozyme Substances 0.000 description 3
235000010335 lysozyme Nutrition 0.000 description 3
239000000047 product Substances 0.000 description 3
238000000926 separation method Methods 0.000 description 3
239000007787 solid Substances 0.000 description 3
CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 2
229930183912 Cytidylic acid Natural products 0.000 description 2
KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 2
102000014150 Interferons Human genes 0.000 description 2
108010050904 Interferons Proteins 0.000 description 2
229910019142 PO4 Inorganic materials 0.000 description 2
UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 description 2
DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 2
230000009471 action Effects 0.000 description 2
238000013019 agitation Methods 0.000 description 2
239000012670 alkaline solution Substances 0.000 description 2
238000003556 assay Methods 0.000 description 2
238000006243 chemical reaction Methods 0.000 description 2
229920001577 copolymer Polymers 0.000 description 2
IERHLVCPSMICTF-UHFFFAOYSA-N cytidine monophosphate Natural products O=C1N=C(N)C=CN1C1C(O)C(O)C(COP(O)(O)=O)O1 IERHLVCPSMICTF-UHFFFAOYSA-N 0.000 description 2
229960001484 edetic acid Drugs 0.000 description 2
DNJIEGIFACGWOD-UHFFFAOYSA-N ethanethiol Chemical compound CCS DNJIEGIFACGWOD-UHFFFAOYSA-N 0.000 description 2
238000004108 freeze drying Methods 0.000 description 2
JMANVNJQNLATNU-UHFFFAOYSA-N glycolonitrile Natural products N#CC#N JMANVNJQNLATNU-UHFFFAOYSA-N 0.000 description 2
238000003306 harvesting Methods 0.000 description 2
239000012535 impurity Substances 0.000 description 2
229910052816 inorganic phosphate Inorganic materials 0.000 description 2
229940079322 interferon Drugs 0.000 description 2
230000004048 modification Effects 0.000 description 2
238000012986 modification Methods 0.000 description 2
229910052757 nitrogen Inorganic materials 0.000 description 2
150000003833 nucleoside derivatives Chemical class 0.000 description 2
239000002773 nucleotide Substances 0.000 description 2
125000003729 nucleotide group Chemical group 0.000 description 2
239000010452 phosphate Substances 0.000 description 2
229920000642 polymer Polymers 0.000 description 2
230000000379 polymerizing effect Effects 0.000 description 2
230000001376 precipitating effect Effects 0.000 description 2
238000000746 purification Methods 0.000 description 2
239000012465 retentate Substances 0.000 description 2
LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 2
DGVVWUTYPXICAM-UHFFFAOYSA-N β‐Mercaptoethanol Chemical compound OCCS DGVVWUTYPXICAM-UHFFFAOYSA-N 0.000 description 2
QFMZQPDHXULLKC-UHFFFAOYSA-N 1,2-bis(diphenylphosphino)ethane Chemical compound C=1C=CC=CC=1P(C=1C=CC=CC=1)CCP(C=1C=CC=CC=1)C1=CC=CC=C1 QFMZQPDHXULLKC-UHFFFAOYSA-N 0.000 description 1
CWGFSQJQIHRAAE-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol tetrahydrochloride Chemical compound Cl.Cl.Cl.Cl.OCC(N)(CO)CO CWGFSQJQIHRAAE-UHFFFAOYSA-N 0.000 description 1
QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 1
239000000061 acid fraction Substances 0.000 description 1
230000004913 activation Effects 0.000 description 1
238000005273 aeration Methods 0.000 description 1
239000003513 alkali Substances 0.000 description 1
150000008044 alkali metal hydroxides Chemical class 0.000 description 1
239000003963 antioxidant agent Substances 0.000 description 1
230000003078 antioxidant effect Effects 0.000 description 1
229940041514 candida albicans extract Drugs 0.000 description 1
239000006285 cell suspension Substances 0.000 description 1
238000003776 cleavage reaction Methods 0.000 description 1
239000000356 contaminant Substances 0.000 description 1
ATDGTVJJHBUTRL-UHFFFAOYSA-N cyanogen bromide Chemical compound BrC#N ATDGTVJJHBUTRL-UHFFFAOYSA-N 0.000 description 1
238000010908 decantation Methods 0.000 description 1
238000006872 enzymatic polymerization reaction Methods 0.000 description 1
238000000605 extraction Methods 0.000 description 1
238000000855 fermentation Methods 0.000 description 1
230000004151 fermentation Effects 0.000 description 1
238000001914 filtration Methods 0.000 description 1
230000000415 inactivating effect Effects 0.000 description 1
230000002779 inactivation Effects 0.000 description 1
239000000411 inducer Substances 0.000 description 1
230000006698 induction Effects 0.000 description 1
229910017053 inorganic salt Inorganic materials 0.000 description 1
239000006166 lysate Substances 0.000 description 1
238000002156 mixing Methods 0.000 description 1
235000015097 nutrients Nutrition 0.000 description 1
230000003287 optical effect Effects 0.000 description 1
239000012071 phase Substances 0.000 description 1
235000021317 phosphate Nutrition 0.000 description 1
229940115272 polyinosinic:polycytidylic acid Drugs 0.000 description 1
239000002244 precipitate Substances 0.000 description 1
238000010791 quenching Methods 0.000 description 1
230000000171 quenching effect Effects 0.000 description 1
238000011084 recovery Methods 0.000 description 1
230000009467 reduction Effects 0.000 description 1
235000017557 sodium bicarbonate Nutrition 0.000 description 1
229910000030 sodium bicarbonate Inorganic materials 0.000 description 1
239000011343 solid material Substances 0.000 description 1
239000002904 solvent Substances 0.000 description 1
239000007858 starting material Substances 0.000 description 1
239000010414 supernatant solution Substances 0.000 description 1
UEUXEKPTXMALOB-UHFFFAOYSA-J tetrasodium;2-[2-[bis(carboxylatomethyl)amino]ethyl-(carboxylatomethyl)amino]acetate Chemical compound [Na+].[Na+].[Na+].[Na+].[O-]C(=O)CN(CC([O-])=O)CCN(CC([O-])=O)CC([O-])=O UEUXEKPTXMALOB-UHFFFAOYSA-J 0.000 description 1
238000001665 trituration Methods 0.000 description 1
238000005406 washing Methods 0.000 description 1
238000005303 weighing Methods 0.000 description 1
239000012138 yeast extract Substances 0.000 description 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
- C12N9/1258—Polyribonucleotide nucleotidyltransferase (2.7.7.8), i.e. polynucleotide phosphorylase
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
- C12N11/10—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a carbohydrate
- C12N11/12—Cellulose or derivatives thereof
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/26—Preparation of nitrogen-containing carbohydrates
- C12P19/28—N-glycosides
- C12P19/30—Nucleotides
- C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides

Landscapes

Life Sciences & Earth Sciences (AREA)
Health & Medical Sciences (AREA)
Chemical & Material Sciences (AREA)
Organic Chemistry (AREA)
Engineering & Computer Science (AREA)
Zoology (AREA)
Wood Science & Technology (AREA)
Bioinformatics & Cheminformatics (AREA)
Genetics & Genomics (AREA)
Molecular Biology (AREA)
Microbiology (AREA)
Biotechnology (AREA)
Biochemistry (AREA)
General Engineering & Computer Science (AREA)
General Health & Medical Sciences (AREA)
Biomedical Technology (AREA)
Medicinal Chemistry (AREA)
Chemical Kinetics & Catalysis (AREA)
General Chemical & Material Sciences (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)
Enzymes And Modification Thereof (AREA)

IL36707A 1970-05-04 1971-04-26 Process for the preparation of polynucleotide phosphorylase IL36707A (en)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
US3455070A	1970-05-04	1970-05-04
US7733470A	1970-10-01	1970-10-01

Publications (2)

Publication Number	Publication Date
IL36707A0 IL36707A0 (en)	1971-06-23
IL36707A true IL36707A (en)	1974-10-22

Family

ID=26711110

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
IL36707A IL36707A (en)	1970-05-04	1971-04-26	Process for the preparation of polynucleotide phosphorylase

Country Status (9)

Country	Link
BE (1)	BE766527A (fr)
CA (1)	CA958666A (fr)
CH (1)	CH553226A (fr)
DE (2)	DE2121809A1 (fr)
FR (1)	FR2091158A5 (fr)
IE (1)	IE35076B1 (fr)
IL (1)	IL36707A (fr)
NL (1)	NL7105073A (fr)
SU (1)	SU396014A3 (fr)

1971
- 1971-04-15 NL NL7105073A patent/NL7105073A/xx unknown
- 1971-04-26 IL IL36707A patent/IL36707A/en unknown
- 1971-04-28 CA CA111,678A patent/CA958666A/en not_active Expired
- 1971-04-28 CH CH626871A patent/CH553226A/xx not_active IP Right Cessation
- 1971-04-29 IE IE531/71A patent/IE35076B1/xx unknown
- 1971-04-30 BE BE766527A patent/BE766527A/fr unknown
- 1971-04-30 SU SU1654637A patent/SU396014A3/ru active
- 1971-05-03 DE DE19712121809 patent/DE2121809A1/de active Pending
- 1971-05-03 DE DE19712144529 patent/DE2144529A1/de active Pending
- 1971-05-04 FR FR7115979A patent/FR2091158A5/fr not_active Expired

Also Published As

Publication number	Publication date
CH553226A (de)	1974-08-30
IE35076L (en)	1971-11-04
FR2091158A5 (en)	1972-01-14
BE766527A (fr)	1971-11-03
CA958666A (en)	1974-12-03
DE2144529A1 (de)	1972-11-30
SU396014A3 (fr)	1973-08-28
IE35076B1 (en)	1975-10-29
NL7105073A (fr)	1971-11-08
DE2121809A1 (de)	1971-12-23
IL36707A0 (en)	1971-06-23

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US4250263A (en)	1981-02-10	Method of purification of thermally stable enzymes
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US3033758A (en)	1962-05-08	Preparation of levans
US4080261A (en)	1978-03-21	Novel endonuclease and process for production thereof
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