IL78543A - Production of interleukin-2 protein by E. ILOC - Google Patents

Production of interleukin-2 protein by E. ILOC

Info

Publication number: IL78543A
Authority: IL; Israel
Prior art keywords: ion source; medium; protein; ifn; escherichia coli
Prior art date: 1985-04-30

Application number

IL7854386A

Other languages

English (en)

Hebrew (he)

Other versions

IL78543A0 (en

Original Assignee

Takeda Chemical Industries Ltd

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1985-04-30

Filing date

1986-04-18

Publication date

1994-11-28

1985-04-30 Priority claimed from PCT/JP1985/000248 external-priority patent/WO1986006410A1/ja

1986-03-03 Priority claimed from JP61045667A external-priority patent/JPH0634746B2/ja

1986-04-18 Application filed by Takeda Chemical Industries Ltd filed Critical Takeda Chemical Industries Ltd

1986-08-31 Publication of IL78543A0 publication Critical patent/IL78543A0/xx

1994-11-28 Publication of IL78543A publication Critical patent/IL78543A/en

Links

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VJLOFJZWUDZJBX-UHFFFAOYSA-N bis(2-hydroxyethyl)azanium;chloride Chemical compound [Cl-].OCC[NH2+]CCO VJLOFJZWUDZJBX-UHFFFAOYSA-N 0.000 description 1
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238000011835 investigation Methods 0.000 description 1
238000005342 ion exchange Methods 0.000 description 1
238000004255 ion exchange chromatography Methods 0.000 description 1
159000000014 iron salts Chemical class 0.000 description 1
BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 description 1
WBJZTOZJJYAKHQ-UHFFFAOYSA-K iron(3+) phosphate Chemical compound [Fe+3].[O-]P([O-])([O-])=O WBJZTOZJJYAKHQ-UHFFFAOYSA-K 0.000 description 1
229910000359 iron(II) sulfate Inorganic materials 0.000 description 1
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229960000310 isoleucine Drugs 0.000 description 1
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239000008101 lactose Substances 0.000 description 1
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239000002523 lectin Substances 0.000 description 1
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239000006166 lysate Substances 0.000 description 1
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230000014759 maintenance of location Effects 0.000 description 1
239000011656 manganese carbonate Substances 0.000 description 1
235000006748 manganese carbonate Nutrition 0.000 description 1
229940093474 manganese carbonate Drugs 0.000 description 1
229940077478 manganese phosphate Drugs 0.000 description 1
229910000016 manganese(II) carbonate Inorganic materials 0.000 description 1
XMWCXZJXESXBBY-UHFFFAOYSA-L manganese(ii) carbonate Chemical compound [Mn+2].[O-]C([O-])=O XMWCXZJXESXBBY-UHFFFAOYSA-L 0.000 description 1
JHKXZYLNVJRAAJ-UHFFFAOYSA-N methionyl-alanine Chemical group CSCCC(N)C(=O)NC(C)C(O)=O JHKXZYLNVJRAAJ-UHFFFAOYSA-N 0.000 description 1
235000019796 monopotassium phosphate Nutrition 0.000 description 1
238000011580 nude mouse model Methods 0.000 description 1
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239000000813 peptide hormone Substances 0.000 description 1
238000012510 peptide mapping method Methods 0.000 description 1
239000000546 pharmaceutical excipient Substances 0.000 description 1
239000008363 phosphate buffer Substances 0.000 description 1
230000001766 physiological effect Effects 0.000 description 1
239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 description 1
235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 description 1
235000011164 potassium chloride Nutrition 0.000 description 1
GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 description 1
LWIHDJKSTIGBAC-UHFFFAOYSA-K potassium phosphate Substances [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 description 1
238000001556 precipitation Methods 0.000 description 1
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125000003607 serino group Chemical group [H]N([H])[C@]([H])(C(=O)[*])C(O[H])([H])[H] 0.000 description 1
229910000029 sodium carbonate Inorganic materials 0.000 description 1
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125000006850 spacer group Chemical group 0.000 description 1
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WPLOVIFNBMNBPD-ATHMIXSHSA-N subtilin Chemical compound CC1SCC(NC2=O)C(=O)NC(CC(N)=O)C(=O)NC(C(=O)NC(CCCCN)C(=O)NC(C(C)CC)C(=O)NC(=C)C(=O)NC(CCCCN)C(O)=O)CSC(C)C2NC(=O)C(CC(C)C)NC(=O)C1NC(=O)C(CCC(N)=O)NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C1NC(=O)C(=C/C)/NC(=O)C(CCC(N)=O)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)CNC(=O)C(NC(=O)C(NC(=O)C2NC(=O)CNC(=O)C3CCCN3C(=O)C(NC(=O)C3NC(=O)C(CC(C)C)NC(=O)C(=C)NC(=O)C(CCC(O)=O)NC(=O)C(NC(=O)C(CCCCN)NC(=O)C(N)CC=4C5=CC=CC=C5NC=4)CSC3)C(C)SC2)C(C)C)C(C)SC1)CC1=CC=CC=C1 WPLOVIFNBMNBPD-ATHMIXSHSA-N 0.000 description 1
239000005720 sucrose Substances 0.000 description 1
239000013589 supplement Substances 0.000 description 1
230000001502 supplementing effect Effects 0.000 description 1
229960002180 tetracycline Drugs 0.000 description 1
229930101283 tetracycline Natural products 0.000 description 1
235000019364 tetracycline Nutrition 0.000 description 1
150000003522 tetracyclines Chemical class 0.000 description 1
238000010257 thawing Methods 0.000 description 1
229940124597 therapeutic agent Drugs 0.000 description 1
125000000341 threoninyl group Chemical group [H]OC([H])(C([H])([H])[H])C([H])(N([H])[H])C(*)=O 0.000 description 1
229940104230 thymidine Drugs 0.000 description 1
230000009466 transformation Effects 0.000 description 1
230000001131 transforming effect Effects 0.000 description 1
230000004614 tumor growth Effects 0.000 description 1
238000000108 ultra-filtration Methods 0.000 description 1
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150000003722 vitamin derivatives Chemical class 0.000 description 1
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
229910000368 zinc sulfate Inorganic materials 0.000 description 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides

Landscapes

Life Sciences & Earth Sciences (AREA)
Chemical & Material Sciences (AREA)
Organic Chemistry (AREA)
Engineering & Computer Science (AREA)
Wood Science & Technology (AREA)
Health & Medical Sciences (AREA)
Zoology (AREA)
General Chemical & Material Sciences (AREA)
Bioinformatics & Cheminformatics (AREA)
Microbiology (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Chemical Kinetics & Catalysis (AREA)
Biotechnology (AREA)
Biochemistry (AREA)
Molecular Biology (AREA)
General Engineering & Computer Science (AREA)
General Health & Medical Sciences (AREA)
Genetics & Genomics (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)
Peptides Or Proteins (AREA)
Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

IL7854386A 1985-04-30 1986-04-18 Production of interleukin-2 protein by E. ILOC IL78543A (en)

Applications Claiming Priority (3)

Application Number	Priority Date	Filing Date	Title
PCT/JP1985/000248 WO1986006410A1 (fr)	1985-04-30	1985-04-30	Procede d'augmentation de la production d'interleucine-2
JP22151785		1985-10-03
JP61045667A JPH0634746B2 (ja)	1985-04-30	1986-03-03	インターロイキンの増収法

Publications (2)

Publication Number	Publication Date
IL78543A0 IL78543A0 (en)	1986-08-31
IL78543A true IL78543A (en)	1994-11-28

Family

ID=27292335

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
IL7854386A IL78543A (en)	1985-04-30	1986-04-18	Production of interleukin-2 protein by E. ILOC

Country Status (10)

Country	Link
EP (1)	EP0200417B1 (de)
CN (1)	CN1012645B (de)
AT (1)	ATE81675T1 (de)
AU (1)	AU603545B2 (de)
CA (1)	CA1273591A (de)
DE (1)	DE3686981T2 (de)
DK (1)	DK196086A (de)
IE (1)	IE58766B1 (de)
IL (1)	IL78543A (de)
NZ (1)	NZ215994A (de)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
DE3532134A1 (de) *	1985-09-10	1987-03-12	Basf Ag	Verfahren zur herstellung von proteinen bestimmter n-terminaler struktur mit hilfe von prokaryonten
EP0315950A3 (de) *	1987-11-09	1990-05-23	Otsuka Pharmaceutical Co., Ltd.	Verfahren zur Züchtung von Zellen, welche rekombinantes Protein exprimieren
CN1062311C (zh) *	1996-12-27	2001-02-21	中国医学科学院基础医学研究所	人白细胞介素2衍生物与人心钠素衍生物的融合基因、其蛋白产物及应用
DE19749259A1 (de) *	1997-11-07	1999-05-12	Dade Behring Marburg Gmbh	Verfahren zur Herstellung von funktionalem rekombinantem Gewebsfaktor
CN102643826A (zh) *	2012-04-10	2012-08-22	上海交通大学	胸腺素β4四联体基因及其在大肠杆菌中表达的方法

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
GB2108510B (en) *	1981-10-03	1984-12-12	Ciba Geigy Ag	Dnas, recombinant dnas, hosts containing them, polypeptides and processes for the production thereof
JPS60118196A (ja) *	1983-11-30	1985-06-25	Takeda Chem Ind Ltd	インタ−フェロンの製造法
US4855238A (en) *	1983-12-16	1989-08-08	Genentech, Inc.	Recombinant gamma interferons having enhanced stability and methods therefor
US4656132A (en) *	1984-03-28	1987-04-07	Cetus Corporation	Method of improving the yield of heterologous protein produced by cultivating recombinant bacteria
DE3532134A1 (de) *	1985-09-10	1987-03-12	Basf Ag	Verfahren zur herstellung von proteinen bestimmter n-terminaler struktur mit hilfe von prokaryonten

1986
- 1986-04-16 EP EP86302833A patent/EP0200417B1/de not_active Expired - Lifetime
- 1986-04-16 DE DE8686302833T patent/DE3686981T2/de not_active Expired - Fee Related
- 1986-04-16 AT AT86302833T patent/ATE81675T1/de active
- 1986-04-16 IE IE99786A patent/IE58766B1/en not_active IP Right Cessation
- 1986-04-18 IL IL7854386A patent/IL78543A/en not_active IP Right Cessation
- 1986-04-23 AU AU56522/86A patent/AU603545B2/en not_active Ceased
- 1986-04-23 CA CA000507408A patent/CA1273591A/en not_active Expired - Lifetime
- 1986-04-29 CN CN86102977A patent/CN1012645B/zh not_active Expired
- 1986-04-29 DK DK196086A patent/DK196086A/da not_active Application Discontinuation
- 1986-04-29 NZ NZ215994A patent/NZ215994A/xx unknown

Also Published As

Publication number	Publication date
AU5652286A (en)	1986-11-06
AU603545B2 (en)	1990-11-22
IL78543A0 (en)	1986-08-31
DE3686981T2 (de)	1993-07-08
EP0200417A2 (de)	1986-11-05
CA1273591A (en)	1990-09-04
DE3686981D1 (de)	1992-11-26
DK196086D0 (da)	1986-04-29
EP0200417B1 (de)	1992-10-21
ATE81675T1 (de)	1992-11-15
EP0200417A3 (en)	1988-08-31
IE58766B1 (en)	1993-11-03
DK196086A (da)	1986-10-31
IE860997L (en)	1986-10-30
NZ215994A (en)	1989-07-27
CN86102977A (zh)	1986-10-29
CN1012645B (zh)	1991-05-22

Legal Events

Date	Code	Title
1996-08-04	KB	Patent renewed
2000-08-31	KB	Patent renewed
2004-06-01	KB	Patent renewed
2006-08-01	EXP	Patent expired

Publication	Publication Date	Title
US5464939A (en)	1995-11-07	Highly purified protein, production and use thereof
US4656132A (en)	1987-04-07	Method of improving the yield of heterologous protein produced by cultivating recombinant bacteria
EP0055945A2 (de)	1982-07-14	Menschliches Proinsulin und dessen Analoge und Verfahren zur Herstellung durch mikrobielle polypeptide Expression und Umwandlung derselben in menschliches Insulin
RU2094439C1 (ru)	1997-10-27	Рекомбинантный полипептид pgh(a) с n-концевым аланином
JP2012024108A (ja)	2012-02-09	培地へのペプチドの直接発現
US4656131A (en)	1987-04-07	Method for producing interferons
IE57053B1 (en)	1992-04-08	Human immune interferon protein and production thereof
CA2179909A1 (en)	1995-07-13	Ligand that binds fas antigen
Bhaduri et al.	1970	Initiation of protein synthesis in rabbit reticulocytes
EP0009930B1 (de)	1982-07-21	Rekombinante DNA, Verfahren zu ihrer Herstellung und Herstellung fremder Proteine durch einzellige Wirte, die sie enthalten
EP0176299B1 (de)	1992-03-18	Gegenseitige Trennung von Proteinen
Ray et al.	2002	Production of salmon calcitonin by direct expression of a glycine-extended precursor in Escherichia coli
CA1286242C (en)	1991-07-16	Mutual separation of proteins
US5256769A (en)	1993-10-26	Mutual separation of proteins
IL78543A (en)	1994-11-28	Production of interleukin-2 protein by E. ILOC
US5210029A (en)	1993-05-11	Method of producing interleukin-2
EP0152483B1 (de)	1989-03-29	Verfahren zur herstellung von peptiden
EP0234592A1 (de)	1987-09-02	Plasmid, das ein DNA-Fragment enthält, welches für die Fc-Region von menschlichem Immunoglobulin G Protein codiert, und Verwendung desselben zur Herstellung des genannten Proteins
KR940011533B1 (ko)	1994-12-20	인터로이킨의 증수법
US4935356A (en)	1990-06-19	Production of interleukin-2
US5700913A (en)	1997-12-23	Unglycosylated human interleukin-2 polypeptides
WO1987004723A1 (en)	1987-08-13	Recombinant human b-cell growth factor
CN102352337B (zh)	2014-04-23	利用异源启动子控制大肠杆菌基因组n-乙酰基转移酶表达重组菌及应用
EP0416505A2 (de)	1991-03-13	Expressionsplasmide und deren Verwendung
CN102558356A (zh)	2012-07-11	一种人胰岛素原融合蛋白及人胰岛素的制备方法