DK196086A - Fremgangsmaade til fremstilling af et protein - Google Patents

Fremgangsmaade til fremstilling af et protein Download PDF

Info

Publication number: DK196086A
Authority: DK; Denmark
Prior art keywords: leu; protein; thr; glu; escherichia coli
Prior art date: 1985-04-30

Application number

DK196086A

Other languages

English (en)

Other versions

DK196086D0 (da

Inventor

Kazuaki Kitano

Shigeru Fujimoto

Original Assignee

Takeda Chemical Industries Ltd

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1985-04-30

Filing date

1986-04-29

Publication date

1986-10-31

1985-04-30 Priority claimed from PCT/JP1985/000248 external-priority patent/WO1986006410A1/ja

1986-03-03 Priority claimed from JP61045667A external-priority patent/JPH0634746B2/ja

1986-04-29 Application filed by Takeda Chemical Industries Ltd filed Critical Takeda Chemical Industries Ltd

1986-04-29 Publication of DK196086D0 publication Critical patent/DK196086D0/da

1986-10-31 Publication of DK196086A publication Critical patent/DK196086A/da

Links

108090000623 proteins and genes Proteins 0.000 title abstract 6
102000004169 proteins and genes Human genes 0.000 title abstract 5
238000004519 manufacturing process Methods 0.000 title abstract 2
238000000034 method Methods 0.000 title abstract 2
IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 abstract 2
241000588724 Escherichia coli Species 0.000 abstract 2
108091081024 Start codon Proteins 0.000 abstract 2
108020004705 Codon Proteins 0.000 abstract 1
FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 abstract 1
WAEMQWOKJMHJLA-UHFFFAOYSA-N Manganese(2+) Chemical compound [Mn+2] WAEMQWOKJMHJLA-UHFFFAOYSA-N 0.000 abstract 1
239000013604 expression vector Substances 0.000 abstract 1
XEEYBQQBJWHFJM-UHFFFAOYSA-N iron Substances [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 abstract 1
229910052742 iron Inorganic materials 0.000 abstract 1
-1 iron ion Chemical class 0.000 abstract 1
229910001437 manganese ion Inorganic materials 0.000 abstract 1
229930182817 methionine Natural products 0.000 abstract 1
125000001360 methionine group Chemical group N[C@@H](CCSC)C(=O)* 0.000 abstract 1
239000000203 mixture Substances 0.000 abstract 1
229910052757 nitrogen Inorganic materials 0.000 abstract 1
108010025306 histidylleucine Proteins 0.000 description 2
WDIYWDJLXOCGRW-ACZMJKKPSA-N Ala-Asp-Glu Chemical compound [H]N[C@@H](C)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CCC(O)=O)C(O)=O WDIYWDJLXOCGRW-ACZMJKKPSA-N 0.000 description 1
FFZJHQODAYHGPO-KZVJFYERSA-N Ala-Pro-Thr Chemical compound C[C@@H](O)[C@@H](C(O)=O)NC(=O)[C@@H]1CCCN1C(=O)[C@H](C)N FFZJHQODAYHGPO-KZVJFYERSA-N 0.000 description 1
LSMDIAAALJJLRO-XQXXSGGOSA-N Ala-Thr-Glu Chemical compound [H]N[C@@H](C)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CCC(O)=O)C(O)=O LSMDIAAALJJLRO-XQXXSGGOSA-N 0.000 description 1
AWXDRZJQCVHCIT-DCAQKATOSA-N Asn-Pro-Lys Chemical compound NCCCC[C@@H](C(O)=O)NC(=O)[C@@H]1CCCN1C(=O)[C@@H](N)CC(N)=O AWXDRZJQCVHCIT-DCAQKATOSA-N 0.000 description 1
QNNBHTFDFFFHGC-KKUMJFAQSA-N Asn-Tyr-Lys Chemical compound C1=CC(=CC=C1C[C@@H](C(=O)N[C@@H](CCCCN)C(=O)O)NC(=O)[C@H](CC(=O)N)N)O QNNBHTFDFFFHGC-KKUMJFAQSA-N 0.000 description 1
KWBQPGIYEZKDEG-FSPLSTOPSA-N Asn-Val Chemical compound CC(C)[C@@H](C(O)=O)NC(=O)[C@@H](N)CC(N)=O KWBQPGIYEZKDEG-FSPLSTOPSA-N 0.000 description 1
AOZBJZBKFHOYHL-AVGNSLFASA-N Cys-Glu-Tyr Chemical compound [H]N[C@@H](CS)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC1=CC=C(O)C=C1)C(O)=O AOZBJZBKFHOYHL-AVGNSLFASA-N 0.000 description 1
XLLSMEFANRROJE-GUBZILKMSA-N Cys-Leu-Glu Chemical compound CC(C)C[C@@H](C(=O)N[C@@H](CCC(=O)O)C(=O)O)NC(=O)[C@H](CS)N XLLSMEFANRROJE-GUBZILKMSA-N 0.000 description 1
MUSGDMDGNGXULI-DCAQKATOSA-N Glu-Glu-Leu Chemical compound CC(C)C[C@@H](C(O)=O)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@@H](N)CCC(O)=O MUSGDMDGNGXULI-DCAQKATOSA-N 0.000 description 1
DGKBSGNCMCLDSL-BYULHYEWSA-N Gly-Ile-Asn Chemical compound CC[C@H](C)[C@@H](C(=O)N[C@@H](CC(=O)N)C(=O)O)NC(=O)CN DGKBSGNCMCLDSL-BYULHYEWSA-N 0.000 description 1
SKYULSWNBYAQMG-IHRRRGAJSA-N His-Leu-Arg Chemical compound [H]N[C@@H](CC1=CNC=N1)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(O)=O SKYULSWNBYAQMG-IHRRRGAJSA-N 0.000 description 1
IBMVEYRWAWIOTN-UHFFFAOYSA-N L-Leucyl-L-Arginyl-L-Proline Natural products CC(C)CC(N)C(=O)NC(CCCN=C(N)N)C(=O)N1CCCC1C(O)=O IBMVEYRWAWIOTN-UHFFFAOYSA-N 0.000 description 1
MLTRLIITQPXHBJ-BQBZGAKWSA-N Leu-Asn Chemical compound CC(C)C[C@H](N)C(=O)N[C@H](C(O)=O)CC(N)=O MLTRLIITQPXHBJ-BQBZGAKWSA-N 0.000 description 1
IGUOAYLTQJLPPD-DCAQKATOSA-N Leu-Asn-Arg Chemical compound CC(C)C[C@H](N)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@H](C(O)=O)CCCN=C(N)N IGUOAYLTQJLPPD-DCAQKATOSA-N 0.000 description 1
WIDZHJTYKYBLSR-DCAQKATOSA-N Leu-Glu-Glu Chemical compound [H]N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCC(O)=O)C(O)=O WIDZHJTYKYBLSR-DCAQKATOSA-N 0.000 description 1
IWTBYNQNAPECCS-AVGNSLFASA-N Leu-Glu-His Chemical compound CC(C)C[C@H](N)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@H](C(O)=O)CC1=CN=CN1 IWTBYNQNAPECCS-AVGNSLFASA-N 0.000 description 1
PDQDCFBVYXEFSD-SRVKXCTJSA-N Leu-Leu-Asp Chemical compound CC(C)C[C@H](N)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(O)=O)C(O)=O PDQDCFBVYXEFSD-SRVKXCTJSA-N 0.000 description 1
LVTJJOJKDCVZGP-QWRGUYRKSA-N Leu-Lys-Gly Chemical compound [H]N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)NCC(O)=O LVTJJOJKDCVZGP-QWRGUYRKSA-N 0.000 description 1
REPBGZHJKYWFMJ-KKUMJFAQSA-N Leu-Lys-His Chemical compound CC(C)C[C@@H](C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC1=CN=CN1)C(=O)O)N REPBGZHJKYWFMJ-KKUMJFAQSA-N 0.000 description 1
ZDJQVSIPFLMNOX-RHYQMDGZSA-N Leu-Thr-Arg Chemical compound CC(C)C[C@H](N)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@H](C(O)=O)CCCN=C(N)N ZDJQVSIPFLMNOX-RHYQMDGZSA-N 0.000 description 1
ODRREERHVHMIPT-OEAJRASXSA-N Leu-Thr-Phe Chemical compound CC(C)C[C@H](N)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@H](C(O)=O)CC1=CC=CC=C1 ODRREERHVHMIPT-OEAJRASXSA-N 0.000 description 1
YBAFDPFAUTYYRW-UHFFFAOYSA-N N-L-alpha-glutamyl-L-leucine Natural products CC(C)CC(C(O)=O)NC(=O)C(N)CCC(O)=O YBAFDPFAUTYYRW-UHFFFAOYSA-N 0.000 description 1
FSXRLASFHBWESK-HOTGVXAUSA-N Phe-Tyr Chemical compound C([C@H](N)C(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(O)=O)C1=CC=CC=C1 FSXRLASFHBWESK-HOTGVXAUSA-N 0.000 description 1
SSSFPISOZOLQNP-GUBZILKMSA-N Pro-Arg-Asp Chemical compound [H]N1CCC[C@H]1C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC(O)=O)C(O)=O SSSFPISOZOLQNP-GUBZILKMSA-N 0.000 description 1
LTFSLKWFMWZEBD-IMJSIDKUSA-N Ser-Asn Chemical compound OC[C@H](N)C(=O)N[C@H](C(O)=O)CC(N)=O LTFSLKWFMWZEBD-IMJSIDKUSA-N 0.000 description 1
GZBKRJVCRMZAST-XKBZYTNZSA-N Ser-Glu-Thr Chemical compound [H]N[C@@H](CO)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H]([C@@H](C)O)C(O)=O GZBKRJVCRMZAST-XKBZYTNZSA-N 0.000 description 1
HDBOEVPDIDDEPC-CIUDSAMLSA-N Ser-Lys-Asn Chemical compound [H]N[C@@H](CO)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(N)=O)C(O)=O HDBOEVPDIDDEPC-CIUDSAMLSA-N 0.000 description 1
XQJCEKXQUJQNNK-ZLUOBGJFSA-N Ser-Ser-Ser Chemical compound OC[C@H](N)C(=O)N[C@@H](CO)C(=O)N[C@@H](CO)C(O)=O XQJCEKXQUJQNNK-ZLUOBGJFSA-N 0.000 description 1
CAJFZCICSVBOJK-SHGPDSBTSA-N Thr-Ala-Thr Chemical compound C[C@@H](O)[C@H](N)C(=O)N[C@@H](C)C(=O)N[C@@H]([C@@H](C)O)C(O)=O CAJFZCICSVBOJK-SHGPDSBTSA-N 0.000 description 1
VRUFCJZQDACGLH-UVOCVTCTSA-N Thr-Leu-Thr Chemical compound [H]N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H]([C@@H](C)O)C(O)=O VRUFCJZQDACGLH-UVOCVTCTSA-N 0.000 description 1
MGJLBZFUXUGMML-VOAKCMCISA-N Thr-Lys-Lys Chemical compound C[C@H]([C@@H](C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)O)N)O MGJLBZFUXUGMML-VOAKCMCISA-N 0.000 description 1
UGFSAPWZBROURT-IXOXFDKPSA-N Thr-Phe-Cys Chemical compound C[C@H]([C@@H](C(=O)N[C@@H](CC1=CC=CC=C1)C(=O)N[C@@H](CS)C(=O)O)N)O UGFSAPWZBROURT-IXOXFDKPSA-N 0.000 description 1
BDYBHQWMHYDRKJ-UNQGMJICSA-N Thr-Phe-Met Chemical compound C[C@H]([C@@H](C(=O)N[C@@H](CC1=CC=CC=C1)C(=O)N[C@@H](CCSC)C(=O)O)N)O BDYBHQWMHYDRKJ-UNQGMJICSA-N 0.000 description 1
FOADDSDHGRFUOC-DZKIICNBSA-N Val-Glu-Phe Chemical compound CC(C)[C@@H](C(=O)N[C@@H](CCC(=O)O)C(=O)N[C@@H](CC1=CC=CC=C1)C(=O)O)N FOADDSDHGRFUOC-DZKIICNBSA-N 0.000 description 1
FEXILLGKGGTLRI-NHCYSSNCSA-N Val-Leu-Asn Chemical compound CC(C)C[C@@H](C(=O)N[C@@H](CC(=O)N)C(=O)O)NC(=O)[C@H](C(C)C)N FEXILLGKGGTLRI-NHCYSSNCSA-N 0.000 description 1
LYERIXUFCYVFFX-GVXVVHGQSA-N Val-Leu-Glu Chemical compound CC(C)C[C@@H](C(=O)N[C@@H](CCC(=O)O)C(=O)O)NC(=O)[C@H](C(C)C)N LYERIXUFCYVFFX-GVXVVHGQSA-N 0.000 description 1
KOSRFJWDECSPRO-UHFFFAOYSA-N alpha-L-glutamyl-L-glutamic acid Natural products OC(=O)CCC(N)C(=O)NC(CCC(O)=O)C(O)=O KOSRFJWDECSPRO-UHFFFAOYSA-N 0.000 description 1
FSXRLASFHBWESK-UHFFFAOYSA-N dipeptide phenylalanyl-tyrosine Natural products C=1C=C(O)C=CC=1CC(C(O)=O)NC(=O)C(N)CC1=CC=CC=C1 FSXRLASFHBWESK-UHFFFAOYSA-N 0.000 description 1
108010055341 glutamyl-glutamic acid Proteins 0.000 description 1
108010090333 leucyl-lysyl-proline Proteins 0.000 description 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides

Landscapes

Life Sciences & Earth Sciences (AREA)
Chemical & Material Sciences (AREA)
Organic Chemistry (AREA)
Engineering & Computer Science (AREA)
Wood Science & Technology (AREA)
Health & Medical Sciences (AREA)
Zoology (AREA)
General Chemical & Material Sciences (AREA)
Bioinformatics & Cheminformatics (AREA)
Microbiology (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Chemical Kinetics & Catalysis (AREA)
Biotechnology (AREA)
Biochemistry (AREA)
Molecular Biology (AREA)
General Engineering & Computer Science (AREA)
General Health & Medical Sciences (AREA)
Genetics & Genomics (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)
Peptides Or Proteins (AREA)
Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Description

Ala Pro Thr Ser Ser Ser Thr Lys Lys Thr Gin Leu Gin 20

Leu Glu His Leu Leu Leu Asp Leu Gin Met lie Leu Asn

Gly Ile Asn Asn Tyr Lys Asn Pro Lys Leu Thr Arg Met 40

Leu Thr Phe Lys Phe Tyr Met Pro Lys Lys Ala Thr Glu 60

Leu Lys His Leu Gin Cys Leu Glu Glu Glu Leu Lys Pro

Leu Glu Glu Val Leu Asn Leu Ala Gin Ser Lys Asn Phe 80

His Leu Arg Pro Arg Asp Leu lie Ser Asn He Asn Val 100

He Val Leu Glu Leu Lys Gly Ser Glu Thr Thr Phe Met

Cys Glu Tyr Ala Asp Glu Thr Ala Thr lie Val Glu Phe 120

Leu Asn Arg Trp lie Thr Phe Cys Gin Ser He lie Ser 133

Thr Leu Thr

DK196086A 1985-04-30 1986-04-29 Fremgangsmaade til fremstilling af et protein DK196086A (da)

Applications Claiming Priority (3)

Application Number	Priority Date	Filing Date	Title
PCT/JP1985/000248 WO1986006410A1 (fr)	1985-04-30	1985-04-30	Procede d'augmentation de la production d'interleucine-2
JP22151785		1985-10-03
JP61045667A JPH0634746B2 (ja)	1985-04-30	1986-03-03	インターロイキンの増収法

Publications (2)

Publication Number	Publication Date
DK196086D0 DK196086D0 (da)	1986-04-29
DK196086A true DK196086A (da)	1986-10-31

Family

ID=27292335

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
DK196086A DK196086A (da)	1985-04-30	1986-04-29	Fremgangsmaade til fremstilling af et protein

Country Status (10)

Country	Link
EP (1)	EP0200417B1 (da)
CN (1)	CN1012645B (da)
AT (1)	ATE81675T1 (da)
AU (1)	AU603545B2 (da)
CA (1)	CA1273591A (da)
DE (1)	DE3686981T2 (da)
DK (1)	DK196086A (da)
IE (1)	IE58766B1 (da)
IL (1)	IL78543A (da)
NZ (1)	NZ215994A (da)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
DE3532134A1 (de) *	1985-09-10	1987-03-12	Basf Ag	Verfahren zur herstellung von proteinen bestimmter n-terminaler struktur mit hilfe von prokaryonten
DK623188A (da) *	1987-11-09	1989-05-10	Otsuka Pharma Co Ltd	Fremgangsmaade til inkubation af rekombinante proteinekspressionsceller
CN1062311C (zh) *	1996-12-27	2001-02-21	中国医学科学院基础医学研究所	人白细胞介素2衍生物与人心钠素衍生物的融合基因、其蛋白产物及应用
DE19749259A1 (de) *	1997-11-07	1999-05-12	Dade Behring Marburg Gmbh	Verfahren zur Herstellung von funktionalem rekombinantem Gewebsfaktor
CN102643826A (zh) *	2012-04-10	2012-08-22	上海交通大学	胸腺素β4四联体基因及其在大肠杆菌中表达的方法

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
GB2108510B (en) *	1981-10-03	1984-12-12	Ciba Geigy Ag	Dnas, recombinant dnas, hosts containing them, polypeptides and processes for the production thereof
JPS60118196A (ja) *	1983-11-30	1985-06-25	Takeda Chem Ind Ltd	インタ−フェロンの製造法
US4855238A (en) *	1983-12-16	1989-08-08	Genentech, Inc.	Recombinant gamma interferons having enhanced stability and methods therefor
US4656132A (en) *	1984-03-28	1987-04-07	Cetus Corporation	Method of improving the yield of heterologous protein produced by cultivating recombinant bacteria
DE3532134A1 (de) *	1985-09-10	1987-03-12	Basf Ag	Verfahren zur herstellung von proteinen bestimmter n-terminaler struktur mit hilfe von prokaryonten

1986
- 1986-04-16 AT AT86302833T patent/ATE81675T1/de active
- 1986-04-16 DE DE8686302833T patent/DE3686981T2/de not_active Expired - Fee Related
- 1986-04-16 EP EP86302833A patent/EP0200417B1/en not_active Expired - Lifetime
- 1986-04-16 IE IE99786A patent/IE58766B1/en not_active IP Right Cessation
- 1986-04-18 IL IL7854386A patent/IL78543A/en not_active IP Right Cessation
- 1986-04-23 AU AU56522/86A patent/AU603545B2/en not_active Ceased
- 1986-04-23 CA CA000507408A patent/CA1273591A/en not_active Expired - Lifetime
- 1986-04-29 DK DK196086A patent/DK196086A/da not_active Application Discontinuation
- 1986-04-29 CN CN86102977A patent/CN1012645B/zh not_active Expired
- 1986-04-29 NZ NZ215994A patent/NZ215994A/xx unknown

Also Published As

Publication number	Publication date
EP0200417A3 (en)	1988-08-31
DK196086D0 (da)	1986-04-29
DE3686981D1 (de)	1992-11-26
AU603545B2 (en)	1990-11-22
IL78543A (en)	1994-11-28
CA1273591A (en)	1990-09-04
EP0200417A2 (en)	1986-11-05
IE860997L (en)	1986-10-30
DE3686981T2 (de)	1993-07-08
ATE81675T1 (de)	1992-11-15
CN1012645B (zh)	1991-05-22
IE58766B1 (en)	1993-11-03
EP0200417B1 (en)	1992-10-21
IL78543A0 (en)	1986-08-31
NZ215994A (en)	1989-07-27
AU5652286A (en)	1986-11-06
CN86102977A (zh)	1986-10-29

Legal Events

Date	Code	Title	Description
2004-11-29	AHS	Application shelved for other reasons than non-payment

Publication	Publication Date	Title
Beg et al.	1985	The primary structure of α‐lactalbumin from camel milk
MD1652G2 (ro)	2001-12-31	Proteină cu activitate de tip citochină, ADN recombinant, codificator pentru această proteină, celule şi microorganisme transformate, vector de expresie, procedeu de obţinere a proteinei şi remediu care conţine această proteină
ATE71408T1 (de)	1992-01-15	Herstellungsverfahren fuer erythropoietin.
NL300013I1 (nl)	2000-10-02	Nieuwe protein met TNF-remmende werking en hun ber
PT77910A (en)	1984-01-01	Synthetic heat-stable enterotoxin polypeptide of escherichia coli and multimers thereof
IL76107A0 (en)	1985-12-31	Method of removing n-terminal amino acid residues from eucaryotic polypeptide analogs and polypeptides produced thereby
DE3486154D1 (de)	1993-07-01	Cardiodilatin, ein neues peptidhormon und verfahren zu seiner herstellung.
Dykes et al.	1976	Conversion of pepsinogen into pepsin is not a one-step process
ATE162201T1 (de)	1998-01-15	Cnp-gen und vorläuferprotein aus schwein
DK659287D0 (da)	1987-12-15	Ekspression af det klonede lysostaphin-gen
ES2054365T3 (es)	1994-08-01	Inhibicion de la via de la regla del n final en celulas vivas.
HU9401989D0 (en)	1994-09-28	A novel human kunitz-type protease inhibitor and variants thereof
IE850614L (en)	1986-09-08	Cloning of interleukin
DK196086A (da)	1986-10-31	Fremgangsmaade til fremstilling af et protein
ACKLIN et al.	1993	Recombinant human brain‐derived neurotrophic factor (rHuBDNF) Disulfide structure and characterization of BDNF expressed in CHO cells
EP0249412A3 (en)	1989-02-22	C-terminal alpha-amidating enzyme and process for production and use thereof
KR900013077A (ko)	1990-09-03	글루카곤의 제조방법
AU8302791A (en)	1992-02-18	Recombinant gene coding for a protein having an endochitinase activity
EP0147175A3 (en)	1987-02-25	Novel basic polypeptides and a method of producing thereof
ATE129744T1 (de)	1995-11-15	Rekombinantes c-terminal alpha amidatierungsenzym von menschlischem thyroidursprung.
DK200200302A (da)	2002-02-27	Rekombinante polypeptider omfattende aminosyresekvensen af moden vævsfaktorinhibitor (TFI), polypeptidfragmenter deraf, ekspressionsvektorer der koder for disse, værtsceller transformeret dermed, og fremgangsmåde til fremstilling af et ........
JPS6474989A (en)	1989-03-20	Dna to code human lysozyme
KR950029356A (ko)	1995-11-22	폴리펩타이드 전구체의 제조방법
IT1049417B (it)	1981-01-20	Procedimento per la produzione di peptide