IL80433A - Protection of plants against viral infection by expression in them of a dna sequence encoding a coat protein of a plant virus - Google Patents

Protection of plants against viral infection by expression in them of a dna sequence encoding a coat protein of a plant virus

Info

Publication number: IL80433A
Authority: IL; Israel
Prior art keywords: plant; virus; promoter; coat protein; dna
Prior art date: 1985-10-29

Application number

IL8043386A

Other languages

English (en)

Inventor

Roger N Beachy

Robert T Fraley

Stephen G Rogers

Original Assignee

Monsanto Co

Univ Washington

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1985-10-29

Filing date

1986-10-28

Publication date

1996-11-14

1986-10-28 Application filed by Monsanto Co, Univ Washington filed Critical Monsanto Co

1996-11-14 Publication of IL80433A publication Critical patent/IL80433A/en

Links

101710132601 Capsid protein Proteins 0.000 title claims abstract description 121
101710094648 Coat protein Proteins 0.000 title claims abstract description 121
101710125418 Major capsid protein Proteins 0.000 title claims abstract description 121
101710141454 Nucleoprotein Proteins 0.000 title claims abstract description 121
101710083689 Probable capsid protein Proteins 0.000 title claims abstract description 121
241000700605 Viruses Species 0.000 title claims abstract description 121
102100021181 Golgi phosphoprotein 3 Human genes 0.000 title claims abstract description 106
108091028043 Nucleic acid sequence Proteins 0.000 title claims abstract description 46
230000009385 viral infection Effects 0.000 title claims abstract description 22
230000014509 gene expression Effects 0.000 title claims description 32
208000036142 Viral infection Diseases 0.000 title description 5
108020004414 DNA Proteins 0.000 claims abstract description 84
238000000034 method Methods 0.000 claims abstract description 64
108091032973 (ribonucleotides)n+m Proteins 0.000 claims abstract description 46
238000004519 manufacturing process Methods 0.000 claims abstract description 36
239000002773 nucleotide Substances 0.000 claims abstract description 27
125000003729 nucleotide group Chemical group 0.000 claims abstract description 27
102000053602 DNA Human genes 0.000 claims abstract description 25
241000196324 Embryophyta Species 0.000 claims description 257
241000723873 Tobacco mosaic virus Species 0.000 claims description 78
108090000623 proteins and genes Proteins 0.000 claims description 49
208000015181 infectious disease Diseases 0.000 claims description 39
235000002637 Nicotiana tabacum Nutrition 0.000 claims description 33
240000003768 Solanum lycopersicum Species 0.000 claims description 25
102000004169 proteins and genes Human genes 0.000 claims description 24
241000724328 Alfalfa mosaic virus Species 0.000 claims description 22
239000013598 vector Substances 0.000 claims description 22
108010058731 nopaline synthase Proteins 0.000 claims description 20
241000208125 Nicotiana Species 0.000 claims description 18
241000701489 Cauliflower mosaic virus Species 0.000 claims description 16
241000724252 Cucumber mosaic virus Species 0.000 claims description 14
235000007688 Lycopersicon esculentum Nutrition 0.000 claims description 13
230000009885 systemic effect Effects 0.000 claims description 11
230000009466 transformation Effects 0.000 claims description 11
244000068988 Glycine max Species 0.000 claims description 10
235000010469 Glycine max Nutrition 0.000 claims description 10
241000709992 Potato virus X Species 0.000 claims description 10
241000209140 Triticum Species 0.000 claims description 10
235000021307 Triticum Nutrition 0.000 claims description 10
240000008042 Zea mays Species 0.000 claims description 8
241000709769 Potato leafroll virus Species 0.000 claims description 7
241000702295 Tomato golden mosaic virus Species 0.000 claims description 7
235000002017 Zea mays subsp mays Nutrition 0.000 claims description 7
238000003780 insertion Methods 0.000 claims description 7
230000037431 insertion Effects 0.000 claims description 7
230000001404 mediated effect Effects 0.000 claims description 6
240000008067 Cucumis sativus Species 0.000 claims description 5
235000010799 Cucumis sativus var sativus Nutrition 0.000 claims description 5
108700001094 Plant Genes Proteins 0.000 claims description 5
244000061456 Solanum tuberosum Species 0.000 claims description 5
235000002595 Solanum tuberosum Nutrition 0.000 claims description 5
230000001580 bacterial effect Effects 0.000 claims description 5
241000589155 Agrobacterium tumefaciens Species 0.000 claims description 4
235000002566 Capsicum Nutrition 0.000 claims description 4
108010066133 D-octopine dehydrogenase Proteins 0.000 claims description 4
108010003581 Ribulose-bisphosphate carboxylase Proteins 0.000 claims description 4
235000016068 Berberis vulgaris Nutrition 0.000 claims description 3
241000335053 Beta vulgaris Species 0.000 claims description 3
241000207199 Citrus Species 0.000 claims description 3
235000009854 Cucurbita moschata Nutrition 0.000 claims description 3
235000009852 Cucurbita pepo Nutrition 0.000 claims description 3
102000003886 Glycoproteins Human genes 0.000 claims description 3
108090000288 Glycoproteins Proteins 0.000 claims description 3
239000006002 Pepper Substances 0.000 claims description 3
235000016761 Piper aduncum Nutrition 0.000 claims description 3
235000017804 Piper guineense Nutrition 0.000 claims description 3
235000008184 Piper nigrum Nutrition 0.000 claims description 3
235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 3
235000020971 citrus fruits Nutrition 0.000 claims description 3
235000005822 corn Nutrition 0.000 claims description 3
239000002689 soil Substances 0.000 claims description 3
235000020354 squash Nutrition 0.000 claims description 3
241000219193 Brassicaceae Species 0.000 claims description 2
241000209504 Poaceae Species 0.000 claims description 2
235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 claims 4
235000009973 maize Nutrition 0.000 claims 4
240000004244 Cucurbita moschata Species 0.000 claims 2
240000005979 Hordeum vulgare Species 0.000 claims 2
235000007340 Hordeum vulgare Nutrition 0.000 claims 2
244000046052 Phaseolus vulgaris Species 0.000 claims 2
235000010627 Phaseolus vulgaris Nutrition 0.000 claims 2
244000203593 Piper nigrum Species 0.000 claims 2
108020005120 Plant DNA Proteins 0.000 claims 2
241000723762 Potato virus Y Species 0.000 claims 2
MXWKCJYCAHVAHK-UHFFFAOYSA-N 2h-pyran-2,3-diol Chemical compound OC1OC=CC=C1O MXWKCJYCAHVAHK-UHFFFAOYSA-N 0.000 claims 1
241000219104 Cucurbitaceae Species 0.000 claims 1
241000220485 Fabaceae Species 0.000 claims 1
241000208292 Solanaceae Species 0.000 claims 1
239000013611 chromosomal DNA Substances 0.000 claims 1
108010083942 mannopine synthase Proteins 0.000 claims 1
YKNYRRVISWJDSR-UHFFFAOYSA-N methyl oxirane-2-carboxylate Chemical compound COC(=O)C1CO1 YKNYRRVISWJDSR-UHFFFAOYSA-N 0.000 claims 1
230000000644 propagated effect Effects 0.000 claims 1
230000001902 propagating effect Effects 0.000 claims 1
230000001172 regenerating effect Effects 0.000 claims 1
230000001131 transforming effect Effects 0.000 abstract 1
239000013612 plasmid Substances 0.000 description 67
210000004027 cell Anatomy 0.000 description 49
239000012634 fragment Substances 0.000 description 49
239000002299 complementary DNA Substances 0.000 description 38
230000009261 transgenic effect Effects 0.000 description 35
108091026890 Coding region Proteins 0.000 description 34
208000024891 symptom Diseases 0.000 description 32
239000013256 coordination polymer Substances 0.000 description 23
235000018102 proteins Nutrition 0.000 description 22
108020004999 messenger RNA Proteins 0.000 description 19
244000061176 Nicotiana tabacum Species 0.000 description 16
108020000999 Viral RNA Proteins 0.000 description 14
238000011081 inoculation Methods 0.000 description 14
210000001938 protoplast Anatomy 0.000 description 14
101900318283 Alfalfa mosaic virus Capsid protein Proteins 0.000 description 13
238000002474 experimental method Methods 0.000 description 13
JUGOREOARAHOCO-UHFFFAOYSA-M acetylcholine chloride Chemical compound [Cl-].CC(=O)OCC[N+](C)(C)C JUGOREOARAHOCO-UHFFFAOYSA-M 0.000 description 12
240000007377 Petunia x hybrida Species 0.000 description 11
241000723811 Soybean mosaic virus Species 0.000 description 10
239000013615 primer Substances 0.000 description 10
102000004190 Enzymes Human genes 0.000 description 9
108090000790 Enzymes Proteins 0.000 description 9
101900259239 Potato virus X Coat protein Proteins 0.000 description 9
238000003776 cleavage reaction Methods 0.000 description 9
229940088598 enzyme Drugs 0.000 description 9
230000007017 scission Effects 0.000 description 9
238000012360 testing method Methods 0.000 description 9
230000029087 digestion Effects 0.000 description 8
108020004705 Codon Proteins 0.000 description 7
102000004594 DNA Polymerase I Human genes 0.000 description 7
108010017826 DNA Polymerase I Proteins 0.000 description 7
230000015572 biosynthetic process Effects 0.000 description 7
108091027551 Cointegrate Proteins 0.000 description 6
208000035240 Disease Resistance Diseases 0.000 description 6
102100034343 Integrase Human genes 0.000 description 6
241000723613 Tomato mosaic virus Species 0.000 description 6
238000004458 analytical method Methods 0.000 description 6
230000000692 anti-sense effect Effects 0.000 description 6
230000002068 genetic effect Effects 0.000 description 6
238000002955 isolation Methods 0.000 description 6
229930027917 kanamycin Natural products 0.000 description 6
229960000318 kanamycin Drugs 0.000 description 6
SBUJHOSQTJFQJX-NOAMYHISSA-N kanamycin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CN)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](N)[C@H](O)[C@@H](CO)O2)O)[C@H](N)C[C@@H]1N SBUJHOSQTJFQJX-NOAMYHISSA-N 0.000 description 6
229930182823 kanamycin A Natural products 0.000 description 6
239000002609 medium Substances 0.000 description 6
238000004114 suspension culture Methods 0.000 description 6
238000003786 synthesis reaction Methods 0.000 description 6
230000003612 virological effect Effects 0.000 description 6
108090000565 Capsid Proteins Proteins 0.000 description 5
241000283973 Oryctolagus cuniculus Species 0.000 description 5
108010092799 RNA-directed DNA polymerase Proteins 0.000 description 5
238000007792 addition Methods 0.000 description 5
125000003275 alpha amino acid group Chemical group 0.000 description 5
239000003990 capacitor Substances 0.000 description 5
238000009396 hybridization Methods 0.000 description 5
239000002054 inoculum Substances 0.000 description 5
239000000047 product Substances 0.000 description 5
238000012163 sequencing technique Methods 0.000 description 5
238000013518 transcription Methods 0.000 description 5
230000035897 transcription Effects 0.000 description 5
108010059892 Cellulase Proteins 0.000 description 4
241000209510 Liliopsida Species 0.000 description 4
TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 4
239000000020 Nitrocellulose Substances 0.000 description 4
108091034117 Oligonucleotide Proteins 0.000 description 4
108010059820 Polygalacturonase Proteins 0.000 description 4
238000013459 approach Methods 0.000 description 4
230000002238 attenuated effect Effects 0.000 description 4
229940106157 cellulase Drugs 0.000 description 4
230000000295 complement effect Effects 0.000 description 4
201000010099 disease Diseases 0.000 description 4
208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 4
238000004520 electroporation Methods 0.000 description 4
108010093305 exopolygalacturonase Proteins 0.000 description 4
239000013604 expression vector Substances 0.000 description 4
238000002347 injection Methods 0.000 description 4
239000007924 injection Substances 0.000 description 4
239000000463 material Substances 0.000 description 4
239000000203 mixture Substances 0.000 description 4
229920001220 nitrocellulos Polymers 0.000 description 4
230000008488 polyadenylation Effects 0.000 description 4
230000008569 process Effects 0.000 description 4
239000011541 reaction mixture Substances 0.000 description 4
239000000725 suspension Substances 0.000 description 4
210000001519 tissue Anatomy 0.000 description 4
238000001262 western blot Methods 0.000 description 4
239000005631 2,4-Dichlorophenoxyacetic acid Substances 0.000 description 3
QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 3
108010014303 DNA-directed DNA polymerase Proteins 0.000 description 3
102000016928 DNA-directed DNA polymerase Human genes 0.000 description 3
102000004163 DNA-directed RNA polymerases Human genes 0.000 description 3
108090000626 DNA-directed RNA polymerases Proteins 0.000 description 3
WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 description 3
206010020649 Hyperkeratosis Diseases 0.000 description 3
206010034133 Pathogen resistance Diseases 0.000 description 3
101000714429 Potato virus X Coat protein Proteins 0.000 description 3
108091081024 Start codon Proteins 0.000 description 3
108020005202 Viral DNA Proteins 0.000 description 3
239000011543 agarose gel Substances 0.000 description 3
238000006243 chemical reaction Methods 0.000 description 3
238000010367 cloning Methods 0.000 description 3
230000000875 corresponding effect Effects 0.000 description 3
238000011161 development Methods 0.000 description 3
230000018109 developmental process Effects 0.000 description 3
108020001507 fusion proteins Proteins 0.000 description 3
102000037865 fusion proteins Human genes 0.000 description 3
239000000499 gel Substances 0.000 description 3
239000001963 growth medium Substances 0.000 description 3
238000003119 immunoblot Methods 0.000 description 3
230000001939 inductive effect Effects 0.000 description 3
239000012528 membrane Substances 0.000 description 3
238000002703 mutagenesis Methods 0.000 description 3
231100000350 mutagenesis Toxicity 0.000 description 3
238000012809 post-inoculation Methods 0.000 description 3
108091008146 restriction endonucleases Proteins 0.000 description 3
238000002741 site-directed mutagenesis Methods 0.000 description 3
239000000243 solution Substances 0.000 description 3
230000014616 translation Effects 0.000 description 3
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
OPIFSICVWOWJMJ-AEOCFKNESA-N 5-bromo-4-chloro-3-indolyl beta-D-galactoside Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1OC1=CNC2=CC=C(Br)C(Cl)=C12 OPIFSICVWOWJMJ-AEOCFKNESA-N 0.000 description 2
229920000936 Agarose Polymers 0.000 description 2
241000894006 Bacteria Species 0.000 description 2
HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 2
241000219112 Cucumis Species 0.000 description 2
235000015510 Cucumis melo subsp melo Nutrition 0.000 description 2
FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 description 2
102000012410 DNA Ligases Human genes 0.000 description 2
108010061982 DNA Ligases Proteins 0.000 description 2
108010066072 DNA modification methylase EcoRI Proteins 0.000 description 2
244000000626 Daucus carota Species 0.000 description 2
235000002767 Daucus carota Nutrition 0.000 description 2
241000588724 Escherichia coli Species 0.000 description 2
LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
101001128156 Homo sapiens Nanos homolog 3 Proteins 0.000 description 2
101001124309 Homo sapiens Nitric oxide synthase, endothelial Proteins 0.000 description 2
102000003960 Ligases Human genes 0.000 description 2
108090000364 Ligases Proteins 0.000 description 2
229930195725 Mannitol Natural products 0.000 description 2
241000219823 Medicago Species 0.000 description 2
235000017587 Medicago sativa ssp. sativa Nutrition 0.000 description 2
206010028980 Neoplasm Diseases 0.000 description 2
102100028452 Nitric oxide synthase, endothelial Human genes 0.000 description 2
108091092724 Noncoding DNA Proteins 0.000 description 2
238000000636 Northern blotting Methods 0.000 description 2
240000007594 Oryza sativa Species 0.000 description 2
235000007164 Oryza sativa Nutrition 0.000 description 2
ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 2
241000710078 Potyvirus Species 0.000 description 2
102000055027 Protein Methyltransferases Human genes 0.000 description 2
108020004682 Single-Stranded DNA Proteins 0.000 description 2
101100314027 Solanum lycopersicum Tm-2(2) gene Proteins 0.000 description 2
241001672648 Vieira Species 0.000 description 2
108010067390 Viral Proteins Proteins 0.000 description 2
150000001413 amino acids Chemical class 0.000 description 2
229960000723 ampicillin Drugs 0.000 description 2
AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 description 2
238000010276 construction Methods 0.000 description 2
RGWHQCVHVJXOKC-SHYZEUOFSA-J dCTP(4-) Chemical compound O=C1N=C(N)C=CN1[C@@H]1O[C@H](COP([O-])(=O)OP([O-])(=O)OP([O-])([O-])=O)[C@@H](O)C1 RGWHQCVHVJXOKC-SHYZEUOFSA-J 0.000 description 2
230000000694 effects Effects 0.000 description 2
238000001962 electrophoresis Methods 0.000 description 2
230000004927 fusion Effects 0.000 description 2
230000012010 growth Effects 0.000 description 2
BPHPUYQFMNQIOC-NXRLNHOXSA-N isopropyl beta-D-thiogalactopyranoside Chemical compound CC(C)S[C@@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O BPHPUYQFMNQIOC-NXRLNHOXSA-N 0.000 description 2
230000003902 lesion Effects 0.000 description 2
229910001629 magnesium chloride Inorganic materials 0.000 description 2
239000000594 mannitol Substances 0.000 description 2
235000010355 mannitol Nutrition 0.000 description 2
239000003550 marker Substances 0.000 description 2
238000012544 monitoring process Methods 0.000 description 2
239000006870 ms-medium Substances 0.000 description 2
239000005022 packaging material Substances 0.000 description 2
239000002245 particle Substances 0.000 description 2
BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 description 2
229920001184 polypeptide Polymers 0.000 description 2
238000002360 preparation method Methods 0.000 description 2
108090000765 processed proteins & peptides Proteins 0.000 description 2
102000004196 processed proteins & peptides Human genes 0.000 description 2
238000012545 processing Methods 0.000 description 2
230000001105 regulatory effect Effects 0.000 description 2
230000010076 replication Effects 0.000 description 2
238000011160 research Methods 0.000 description 2
230000004044 response Effects 0.000 description 2
235000009566 rice Nutrition 0.000 description 2
239000000523 sample Substances 0.000 description 2
229960000268 spectinomycin Drugs 0.000 description 2
UNFWWIHTNXNPBV-WXKVUWSESA-N spectinomycin Chemical compound O([C@@H]1[C@@H](NC)[C@@H](O)[C@H]([C@@H]([C@H]1O1)O)NC)[C@]2(O)[C@H]1O[C@H](C)CC2=O UNFWWIHTNXNPBV-WXKVUWSESA-N 0.000 description 2
UCSJYZPVAKXKNQ-HZYVHMACSA-N streptomycin Chemical compound CN[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@H]1O[C@@H]1[C@](C=O)(O)[C@H](C)O[C@H]1O[C@@H]1[C@@H](NC(N)=N)[C@H](O)[C@@H](NC(N)=N)[C@H](O)[C@H]1O UCSJYZPVAKXKNQ-HZYVHMACSA-N 0.000 description 2
238000013519 translation Methods 0.000 description 2
235000020138 yakult Nutrition 0.000 description 2
102000040650 (ribonucleotides)n+m Human genes 0.000 description 1
102000002260 Alkaline Phosphatase Human genes 0.000 description 1
108020004774 Alkaline Phosphatase Proteins 0.000 description 1
USFZMSVCRYTOJT-UHFFFAOYSA-N Ammonium acetate Chemical compound N.CC(O)=O USFZMSVCRYTOJT-UHFFFAOYSA-N 0.000 description 1
239000005695 Ammonium acetate Substances 0.000 description 1
241000208173 Apiaceae Species 0.000 description 1
240000007087 Apium graveolens Species 0.000 description 1
235000015849 Apium graveolens Dulce Group Nutrition 0.000 description 1
235000010591 Appio Nutrition 0.000 description 1
101100508919 Arabidopsis thaliana IPT6 gene Proteins 0.000 description 1
241000271566 Aves Species 0.000 description 1
240000002791 Brassica napus Species 0.000 description 1
240000007124 Brassica oleracea Species 0.000 description 1
235000003899 Brassica oleracea var acephala Nutrition 0.000 description 1
235000011299 Brassica oleracea var botrytis Nutrition 0.000 description 1
235000011301 Brassica oleracea var capitata Nutrition 0.000 description 1
235000001169 Brassica oleracea var oleracea Nutrition 0.000 description 1
240000003259 Brassica oleracea var. botrytis Species 0.000 description 1
235000004977 Brassica sinapistrum Nutrition 0.000 description 1
101150083464 CP gene Proteins 0.000 description 1
UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 1
240000001980 Cucurbita pepo Species 0.000 description 1
LMKYZBGVKHTLTN-NKWVEPMBSA-N D-nopaline Chemical compound NC(=N)NCCC[C@@H](C(O)=O)N[C@@H](C(O)=O)CCC(O)=O LMKYZBGVKHTLTN-NKWVEPMBSA-N 0.000 description 1
239000003155 DNA primer Substances 0.000 description 1
KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
241000283074 Equus asinus Species 0.000 description 1
241000701959 Escherichia virus Lambda Species 0.000 description 1
108091029865 Exogenous DNA Proteins 0.000 description 1
241001051053 Garcinia cowa Species 0.000 description 1
108700039691 Genetic Promoter Regions Proteins 0.000 description 1
WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
229920002527 Glycogen Polymers 0.000 description 1
241000238631 Hexapoda Species 0.000 description 1
PMMYEEVYMWASQN-DMTCNVIQSA-N Hydroxyproline Chemical compound O[C@H]1CN[C@H](C(O)=O)C1 PMMYEEVYMWASQN-DMTCNVIQSA-N 0.000 description 1
241000257303 Hymenoptera Species 0.000 description 1
101710203526 Integrase Proteins 0.000 description 1
108091092195 Intron Proteins 0.000 description 1
101710138460 Leaf protein Proteins 0.000 description 1
235000010624 Medicago sativa Nutrition 0.000 description 1
240000007298 Megathyrsus maximus Species 0.000 description 1
241001465754 Metazoa Species 0.000 description 1
101150005851 NOS gene Proteins 0.000 description 1
241000233855 Orchidaceae Species 0.000 description 1
240000004370 Pastinaca sativa Species 0.000 description 1
235000017769 Pastinaca sativa subsp sativa Nutrition 0.000 description 1
101000899841 Pectobacterium parmentieri Endoglucanase S Proteins 0.000 description 1
244000038248 Pennisetum spicatum Species 0.000 description 1
235000007195 Pennisetum typhoides Nutrition 0.000 description 1
240000003889 Piper guineense Species 0.000 description 1
241000758706 Piperaceae Species 0.000 description 1
101710124239 Poly(A) polymerase Proteins 0.000 description 1
235000015696 Portulacaria afra Nutrition 0.000 description 1
241000710007 Potexvirus Species 0.000 description 1
108700040121 Protein Methyltransferases Proteins 0.000 description 1
244000088415 Raphanus sativus Species 0.000 description 1
235000006140 Raphanus sativus var sativus Nutrition 0.000 description 1
MJNIWUJSIGSWKK-UHFFFAOYSA-N Riboflavine 2',3',4',5'-tetrabutanoate Chemical compound CCCC(=O)OCC(OC(=O)CCC)C(OC(=O)CCC)C(OC(=O)CCC)CN1C2=CC(C)=C(C)C=C2N=C2C1=NC(=O)NC2=O MJNIWUJSIGSWKK-UHFFFAOYSA-N 0.000 description 1
240000000111 Saccharum officinarum Species 0.000 description 1
235000007201 Saccharum officinarum Nutrition 0.000 description 1
101100425597 Solanum lycopersicum Tm-1 gene Proteins 0.000 description 1
108091027544 Subgenomic mRNA Proteins 0.000 description 1
206010042566 Superinfection Diseases 0.000 description 1
108700005078 Synthetic Genes Proteins 0.000 description 1
108020005038 Terminator Codon Proteins 0.000 description 1
108700009124 Transcription Initiation Site Proteins 0.000 description 1
241000219793 Trifolium Species 0.000 description 1
244000177175 Typha elephantina Species 0.000 description 1
235000018747 Typha elephantina Nutrition 0.000 description 1
108091023045 Untranslated Region Proteins 0.000 description 1
235000007244 Zea mays Nutrition 0.000 description 1
FJJCIZWZNKZHII-UHFFFAOYSA-N [4,6-bis(cyanoamino)-1,3,5-triazin-2-yl]cyanamide Chemical compound N#CNC1=NC(NC#N)=NC(NC#N)=N1 FJJCIZWZNKZHII-UHFFFAOYSA-N 0.000 description 1
230000004075 alteration Effects 0.000 description 1
XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 1
229910052782 aluminium Inorganic materials 0.000 description 1
125000000539 amino acid group Chemical group 0.000 description 1
229940043376 ammonium acetate Drugs 0.000 description 1
235000019257 ammonium acetate Nutrition 0.000 description 1
239000000427 antigen Substances 0.000 description 1
239000007900 aqueous suspension Substances 0.000 description 1
238000004166 bioassay Methods 0.000 description 1
230000004071 biological effect Effects 0.000 description 1
238000009395 breeding Methods 0.000 description 1
230000001488 breeding effect Effects 0.000 description 1
238000010804 cDNA synthesis Methods 0.000 description 1
239000001110 calcium chloride Substances 0.000 description 1
229910001628 calcium chloride Inorganic materials 0.000 description 1
235000011148 calcium chloride Nutrition 0.000 description 1
244000309466 calf Species 0.000 description 1
108010079058 casein hydrolysate Proteins 0.000 description 1
238000004113 cell culture Methods 0.000 description 1
238000012512 characterization method Methods 0.000 description 1
210000000349 chromosome Anatomy 0.000 description 1
235000020197 coconut milk Nutrition 0.000 description 1
230000000052 comparative effect Effects 0.000 description 1
230000002596 correlated effect Effects 0.000 description 1
238000005520 cutting process Methods 0.000 description 1
238000012217 deletion Methods 0.000 description 1
230000037430 deletion Effects 0.000 description 1
238000001514 detection method Methods 0.000 description 1
230000001627 detrimental effect Effects 0.000 description 1
PMMYEEVYMWASQN-UHFFFAOYSA-N dl-hydroxyproline Natural products OC1C[NH2+]C(C([O-])=O)C1 PMMYEEVYMWASQN-UHFFFAOYSA-N 0.000 description 1
231100000673 dose–response relationship Toxicity 0.000 description 1
-1 electroporation Substances 0.000 description 1
238000001976 enzyme digestion Methods 0.000 description 1
ZMMJGEGLRURXTF-UHFFFAOYSA-N ethidium bromide Chemical compound [Br-].C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CC)=C1C1=CC=CC=C1 ZMMJGEGLRURXTF-UHFFFAOYSA-N 0.000 description 1
229960005542 ethidium bromide Drugs 0.000 description 1
241001233957 eudicotyledons Species 0.000 description 1
238000000605 extraction Methods 0.000 description 1
238000010265 fast atom bombardment Methods 0.000 description 1
239000011888 foil Substances 0.000 description 1
230000005714 functional activity Effects 0.000 description 1
238000010353 genetic engineering Methods 0.000 description 1
239000008103 glucose Substances 0.000 description 1
229940096919 glycogen Drugs 0.000 description 1
238000000227 grinding Methods 0.000 description 1
208000037824 growth disorder Diseases 0.000 description 1
ZRALSGWEFCBTJO-UHFFFAOYSA-N guanidine group Chemical group NC(=N)N ZRALSGWEFCBTJO-UHFFFAOYSA-N 0.000 description 1
229960002591 hydroxyproline Drugs 0.000 description 1
210000000987 immune system Anatomy 0.000 description 1
238000010348 incorporation Methods 0.000 description 1
230000006698 induction Effects 0.000 description 1
230000002458 infectious effect Effects 0.000 description 1
230000001524 infective effect Effects 0.000 description 1
239000003999 initiator Substances 0.000 description 1
101150066555 lacZ gene Proteins 0.000 description 1
239000002502 liposome Substances 0.000 description 1
239000007788 liquid Substances 0.000 description 1
230000004048 modification Effects 0.000 description 1
238000012986 modification Methods 0.000 description 1
230000001338 necrotic effect Effects 0.000 description 1
210000004940 nucleus Anatomy 0.000 description 1
230000031787 nutrient reservoir activity Effects 0.000 description 1
235000015097 nutrients Nutrition 0.000 description 1
244000052769 pathogen Species 0.000 description 1
230000007170 pathology Effects 0.000 description 1
238000007747 plating Methods 0.000 description 1
229910052697 platinum Inorganic materials 0.000 description 1
229920002401 polyacrylamide Polymers 0.000 description 1
238000001556 precipitation Methods 0.000 description 1
230000037452 priming Effects 0.000 description 1
230000017854 proteolysis Effects 0.000 description 1
230000008929 regeneration Effects 0.000 description 1
238000011069 regeneration method Methods 0.000 description 1
230000008439 repair process Effects 0.000 description 1
239000012723 sample buffer Substances 0.000 description 1
229920006395 saturated elastomer Polymers 0.000 description 1
238000012216 screening Methods 0.000 description 1
230000035945 sensitivity Effects 0.000 description 1
238000000926 separation method Methods 0.000 description 1
210000002966 serum Anatomy 0.000 description 1
229910010271 silicon carbide Inorganic materials 0.000 description 1
FQENQNTWSFEDLI-UHFFFAOYSA-J sodium diphosphate Chemical compound [Na+].[Na+].[Na+].[Na+].[O-]P([O-])(=O)OP([O-])([O-])=O FQENQNTWSFEDLI-UHFFFAOYSA-J 0.000 description 1
239000012064 sodium phosphate buffer Substances 0.000 description 1
229940048086 sodium pyrophosphate Drugs 0.000 description 1
241000894007 species Species 0.000 description 1
230000000087 stabilizing effect Effects 0.000 description 1
238000010186 staining Methods 0.000 description 1
238000010561 standard procedure Methods 0.000 description 1
229960005322 streptomycin Drugs 0.000 description 1
230000036435 stunted growth Effects 0.000 description 1
239000000126 substance Substances 0.000 description 1
230000009044 synergistic interaction Effects 0.000 description 1
235000019818 tetrasodium diphosphate Nutrition 0.000 description 1
239000001577 tetrasodium phosphonato phosphate Substances 0.000 description 1
210000001541 thymus gland Anatomy 0.000 description 1
238000004448 titration Methods 0.000 description 1
101150058668 tra2 gene Proteins 0.000 description 1
FGMPLJWBKKVCDB-UHFFFAOYSA-N trans-L-hydroxy-proline Natural products ON1CCCC1C(O)=O FGMPLJWBKKVCDB-UHFFFAOYSA-N 0.000 description 1
UZKQTCBAMSWPJD-UQCOIBPSSA-N trans-Zeatin Natural products OCC(/C)=C\CNC1=NC=NC2=C1N=CN2 UZKQTCBAMSWPJD-UQCOIBPSSA-N 0.000 description 1
UZKQTCBAMSWPJD-FARCUNLSSA-N trans-zeatin Chemical compound OCC(/C)=C/CNC1=NC=NC2=C1N=CN2 UZKQTCBAMSWPJD-FARCUNLSSA-N 0.000 description 1
230000002103 transcriptional effect Effects 0.000 description 1
230000014621 translational initiation Effects 0.000 description 1
108700026220 vif Genes Proteins 0.000 description 1
230000009447 viral pathogenesis Effects 0.000 description 1
230000003442 weekly effect Effects 0.000 description 1
229940023877 zeatin Drugs 0.000 description 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
- C12N15/1131—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against viruses
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
- C12N15/8283—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for virus resistance
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
- C12N2750/00011—Details
- C12N2750/12011—Geminiviridae
- C12N2750/12022—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
- C12N2770/00011—Details
- C12N2770/14011—Bromoviridae
- C12N2770/14022—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
- C12N2770/00011—Details
- C12N2770/40011—Tymoviridae
- C12N2770/40022—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

Landscapes

Health & Medical Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Genetics & Genomics (AREA)
Engineering & Computer Science (AREA)
Chemical & Material Sciences (AREA)
Organic Chemistry (AREA)
Biomedical Technology (AREA)
Zoology (AREA)
Biotechnology (AREA)
General Engineering & Computer Science (AREA)
Bioinformatics & Cheminformatics (AREA)
Molecular Biology (AREA)
Wood Science & Technology (AREA)
Biochemistry (AREA)
Biophysics (AREA)
General Health & Medical Sciences (AREA)
Microbiology (AREA)
Plant Pathology (AREA)
Physics & Mathematics (AREA)
Virology (AREA)
Cell Biology (AREA)
Gastroenterology & Hepatology (AREA)
Medicinal Chemistry (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)
Agricultural Chemicals And Associated Chemicals (AREA)
Saccharide Compounds (AREA)
Compounds Of Unknown Constitution (AREA)

IL8043386A 1985-10-29 1986-10-28 Protection of plants against viral infection by expression in them of a dna sequence encoding a coat protein of a plant virus IL80433A (en)

Applications Claiming Priority (3)

Application Number	Priority Date	Filing Date	Title
US79238985A	1985-10-29	1985-10-29
US84491886A	1986-03-27	1986-03-27
US06/917,027 US6608241B1 (en)	1985-10-29	1986-10-09	Protection of plants against viral infection

Publications (1)

Publication Number	Publication Date
IL80433A true IL80433A (en)	1996-11-14

Family

ID=27419896

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
IL8043386A IL80433A (en)	1985-10-29	1986-10-28	Protection of plants against viral infection by expression in them of a dna sequence encoding a coat protein of a plant virus

Country Status (13)

Country	Link
US (1)	US6608241B1 (de)
EP (1)	EP0223452B1 (de)
JP (4)	JPH06339379A (de)
AT (1)	ATE136330T1 (de)
AU (1)	AU608204B2 (de)
BG (1)	BG49720A3 (de)
CA (1)	CA1341565C (de)
DE (1)	DE3650507T2 (de)
DK (1)	DK515186A (de)
ES (1)	ES2087055T3 (de)
GR (1)	GR3020438T3 (de)
IE (1)	IE81098B1 (de)
IL (1)	IL80433A (de)

Families Citing this family (76)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
ATE201444T1 (de) *	1985-03-21	2001-06-15	Johnston Stephen Ph D	Vom parasit abgeleiteter widerstand
GB8601680D0 (en) *	1986-01-23	1986-02-26	Agricultural Genetics Co	Modification of plant viruses
US5175102A (en) *	1986-01-23	1992-12-29	Agricultural Genetics Company, Limited	Modification of plant viruses or their effects
US5453566A (en) *	1986-03-28	1995-09-26	Calgene, Inc.	Antisense regulation of gene expression in plant/cells
NZ219472A (en) *	1986-03-28	1990-08-28	Calgene Inc	Regulation of phenotype in plant cells using dsdna constructs
US5107065A (en) *	1986-03-28	1992-04-21	Calgene, Inc.	Anti-sense regulation of gene expression in plant cells
DE3751393T2 (de) *	1986-04-02	1996-02-22	Pioneer Hi Bred Int	Pflanzen, resistent gegen den Anti-sens RNS aufweisenden Virus.
DE3751099T3 (de) *	1986-04-02	2001-08-02	Pioneer Hi-Bred International, Inc.	Gegen Viren resistente Pflanzen mit einem viralen Überzugsprotein.
ES2060646T3 (es) *	1987-02-09	1994-12-01	Lubrizol Genetics Inc	Virus rna hibrido.
DE3865694D1 (de) *	1987-02-20	1991-11-28	Takeda Chemical Industries Ltd	Gegen "cucumber mosaic virus"-infektion resistente pflanzen.
ES2165345T3 (es) *	1987-07-10	2002-03-16	Syngenta Participations Ag	Resistencia virica inducible en plantas.
US5623066A (en) *	1987-12-21	1997-04-22	Asgrow Seed Company	Cucumber mosaic virus coat protein gene
AU2927689A (en) *	1987-12-21	1989-07-19	Seminis Vegetable Seeds, Inc.	Cucumber mosaic virus coat protein gene
FR2631973B1 (fr) *	1988-05-30	1992-12-11	Agronomique Inst Nat Rech	Sequence nucleotidique complete de l'adn complementaire de l'arn genomique de potyvirus, genes codant pour la proteine de capside de potyvirus et applications de ces genes a la creation de plantes transgeniques resistantes aux potyvirus
KR900702041A (ko) *	1988-08-19	1990-12-05	로버트 에이. 아미테이지	식물의 발현 카셋트
JPH04500154A (ja) *	1988-08-19	1992-01-16	アズグロウ・シード・カンパニー	キュウリモザイクウイルス外皮蛋白遺伝子
GB8827094D0 (en) *	1988-11-19	1988-12-21	Scottish Crop Research Inst	Dna sequence encoding coat protein gene of potato leafroll virus
EP1103616A3 (de)	1989-02-24	2001-06-27	Monsanto Company	Synthetische Pflanzengene und Verfahren zu ihrer Herstellung
US5428144A (en) *	1989-04-07	1995-06-27	Pioneer Hi-Bred International, Inc.	Maize dwarf mosaic virus cDNA
DE69014644T2 (de) *	1989-09-20	1995-05-04	Upjohn Co	Somatische embryogenese von kürbissen.
HUT57265A (en) *	1989-11-03	1991-11-28	Zaadunie Bv	Process for producing plants of diminished infection-sensitivity
US5939600A (en) *	1989-11-03	1999-08-17	Goldbach; Robert Willem	Nucleic acids encoding tospovirus genome and expression thereof
JPH04144685A (ja) *	1989-11-28	1992-05-19	Nippon Oil Co Ltd	ＯＲＳＶ遺伝子の↓ｃＤＮＡ
DE69114275T2 (de) *	1990-03-02	1996-06-13	Biocem	Regenerierung und genetische transformation der zuckerrübe.
FR2658988A1 (fr) *	1990-03-02	1991-09-06	Biosem	Betterave resistante a la rhizomanie.
US5514570A (en) *	1991-08-27	1996-05-07	Cornell Research Foundation	Squash mosaic virus genes and plants transformed therewith
HU9401401D0 (en) *	1992-01-08	1994-08-29	Sandoz Ag	Virus resistant corn plants
US5569828A (en) *	1993-03-24	1996-10-29	Pioneer Hi-Bred International, Inc.	Maize chlorotic dwarf virus and resistance thereto
JPH09503382A (ja) *	1993-07-09	1997-04-08	アズグロウ・シード・カンパニー	レタス感染性黄化ウイルス遺伝子
GB9505907D0 (en) *	1995-03-23	1995-05-10	Sandoz Ltd	Improvements in or relating to organic compounds
AU5196898A (en) *	1996-11-04	1998-05-29	Board Of Trustees Of The University Of Kentucky	Coat protein mediated resistance in plants
GB9703146D0 (en)	1997-02-14	1997-04-02	Innes John Centre Innov Ltd	Methods and means for gene silencing in transgenic plants
GB9827152D0 (en)	1998-07-03	1999-02-03	Devgen Nv	Characterisation of gene function using double stranded rna inhibition
JP4446085B2 (ja)	2001-10-05	2010-04-07	独立行政法人産業技術総合研究所	フェノールオキシダーゼ遺伝子を導入した植物、及び該植物によるフェノールオキシダーゼの生産方法
US7371848B2 (en)	2004-01-20	2008-05-13	Monsanto Technology Llc	Chimeric promoters comprising a caulimovirus promoter enhancer for use in plants
NZ582988A (en)	2004-10-21	2011-07-29	Venganza Inc	Methods and materials for conferring resistance to pests and pathogens of plants
US7838729B2 (en)	2007-02-26	2010-11-23	Monsanto Technology Llc	Chloroplast transit peptides for efficient targeting of DMO and uses thereof
SI2262896T1 (sl)	2008-04-07	2014-05-30	Monsanto Technology Llc	Rastlinski regulatorni elementi in njihove uporabe
US20120277117A1 (en)	2009-02-27	2012-11-01	Adel Zayed	Hydroponic apparatus and methods of use
US20100257634A1 (en) *	2009-04-03	2010-10-07	Venganza Inc.	Bioassay for gene silencing constructs
US8937214B2 (en)	2009-10-23	2015-01-20	Monsanto Technology Llc	Methods and compositions for expression of transgenes in plants
BR112012019582B1 (pt)	2010-01-14	2021-09-21	Monsanto Technology Llc	Molécula de dna compreendendo elementos reguladores de plantas
KR20140016865A (ko)	2010-08-30	2014-02-10	다우 아그로사이언시즈 엘엘씨	사탕수수 간균형 바이러스(scbv) 인핸서 및 식물 기능유전체학에서의 그의 용도
CA3004033C (en)	2011-03-25	2020-08-18	Monsanto Technology Llc	Plant regulatory elements and uses thereof
UY39077A (es)	2011-05-13	2021-03-26	Monsanto Technology Llc	Moléculas de adn reguladoras de la expresión génica de plantas; construcciones, plantas y sus partes, y productos que las comprenden
RU2639517C2 (ru)	2012-02-29	2017-12-21	ДАУ АГРОСАЙЕНСИЗ ЭлЭлСи	Энхансер палочковидного вируса сахарного тростника (scbv) и его применение в функциональной геномике растений
US9663793B2 (en)	2012-04-20	2017-05-30	Monsanto Technology, Llc	Plant regulatory elements and uses thereof
CN104995301B (zh)	2012-12-19	2018-07-20	孟山都技术公司	植物调控元件及其用途
EP2966981B1 (de)	2013-03-14	2019-12-04	Monsanto Technology LLC	Pflanzenregulierungselemente und verwendungen davon
BR122020011549B1 (pt)	2013-03-14	2021-10-13	Monsanto Technology Llc	Cassete de expressão compreendendo elemento regulador de planta e método para produzir uma planta transgênica
US11519011B2 (en)	2015-03-26	2022-12-06	The Texas A&M University System	Conversion of lignin into bioplastics and lipid fuels
NZ745763A (en)	2016-03-11	2022-08-26	Monsanto Technology Llc	Plant regulatory elements and uses thereof
MX381178B (es)	2016-05-24	2025-03-12	Monsanto Technology Llc	Elementos reguladores de plantas y sus usos
US20190359992A1 (en)	2016-06-28	2019-11-28	Cellectis	Altering expression of gene products in plants through targeted insertion of nucleic acid sequences
CR20210121A (es)	2017-01-19	2021-04-27	Monsanto Technology Llc	Elementos regulatorios de las plantas y sus usos (divisional de exp. 2019-377)
US11236355B2 (en) *	2017-08-22	2022-02-01	The Regents Of The University Of California	ROQ1 provides resistance to both xanthomonas and pseudomonas in plants
US20200332311A1 (en)	2018-01-12	2020-10-22	The Texas A&M University System	Increasing plant bioproduct yield
CN119040379A (zh)	2019-01-10	2024-11-29	孟山都技术公司	植物调控元件和其用途
US20250188505A1 (en)	2021-03-26	2025-06-12	Flagship Pioneering Innovations Vii, Llc	Production of circular polyribonucleotides in a prokaryotic system
TW202300650A (zh)	2021-03-26	2023-01-01	美商旗艦先鋒創新有限責任（Ｖｉｉ）公司	真核系統中環狀多核糖核苷酸的產生
AR125216A1 (es)	2021-03-26	2023-06-28	Flagship Pioneering Innovations Vii Llc	Producción de polirribonucleótidos circulares en un sistema eucariota
PY2294525A (es)	2021-11-01	2024-07-11	Flagship Pioneering Innovations Vii Llc	Polinucleótidos para modificar organismos
CA3247488A1 (en)	2022-01-20	2023-07-27	Flagship Pioneering Innovations Vii, Llc	POLYNUCLEOTIDES FOR MODIFYING ORGANISMS
CA3267072A1 (en)	2022-09-09	2024-03-14	Friedrich Alexander Universität Erlangen-Nürnberg	REGULATORY ELEMENTS IN PLANTS AND THEIR USES
WO2024229359A2 (en)	2023-05-03	2024-11-07	Flagship Pioneering Innovations Vii, Llc	Artificial tymovirales satellite rnas
EP4705478A1 (de)	2023-05-03	2026-03-11	Flagship Pioneering Innovations VII, LLC	Künstliche morellivirale satelliten-rnas
CN121816422A (zh)	2023-05-03	2026-04-07	旗舰创业创新第七有限责任公司	人工混合病毒属卫星rna
CN121712898A (zh)	2023-05-03	2026-03-20	旗舰创业创新第七有限责任公司	用于植物的内源核糖核酸病毒卫星rna扩增系统
EP4705471A1 (de)	2023-05-03	2026-03-11	Flagship Pioneering Innovations VII, LLC	Künstliche ghabrivirale satelliten-rnas
CN121794387A (zh)	2023-05-03	2026-04-03	旗舰创业创新第七有限责任公司	人工伴生豇豆花叶病毒科卫星rna
CN122180776A (zh)	2023-05-03	2026-06-09	旗舰创业创新第七有限责任公司	人工番茄丛矮病毒科卫星rna
EP4705475A1 (de)	2023-05-03	2026-03-11	Flagship Pioneering Innovations VII, LLC	Teilitivirale satelliten-rna-amplifikationssysteme für pflanzen
CN121666453A (zh)	2023-05-03	2026-03-13	旗舰创业创新第七有限责任公司	人工南方菜豆花叶病病毒科卫星rna
US20250075226A1 (en)	2023-08-29	2025-03-06	University Of Freiburg	Proteins for regulation of symbiotic infection and associated regulatory elements
WO2026006779A1 (en)	2024-06-27	2026-01-02	The Regents Of The University Of California	Transgenic plants expressing fern rapid non-photochemical quenching (npq) proteins
CN119709814B (zh) *	2025-02-26	2025-07-18	青岛农业大学	一种兼抗pvy、pvx和cmv的三价弱毒疫苗及应用

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US4407956A (en) *	1981-03-13	1983-10-04	The Regents Of The University Of California	Cloned cauliflower mosaic virus DNA as a plant vehicle
US4527287A (en)	1983-06-24	1985-07-09	Figgie International Inc.	Baseball glove or mitt
EP0178314A1 (de)	1984-04-26	1986-04-23	The Australian National University	Klonierungsverfahren für doppelstrang-dns und dessen verwendung bei der herstellung viraler genklone
ATE201444T1 (de)	1985-03-21	2001-06-15	Johnston Stephen Ph D	Vom parasit abgeleiteter widerstand
US5580716A (en)	1985-03-21	1996-12-03	Stephen A. Johnston	Parasite-derived resistance
US6617496B1 (en)	1985-10-16	2003-09-09	Monsanto Company	Effecting virus resistance in plants through the use of negative strand RNAs
GB8601680D0 (en)	1986-01-23	1986-02-26	Agricultural Genetics Co	Modification of plant viruses
DE3751393T2 (de)	1986-04-02	1996-02-22	Pioneer Hi Bred Int	Pflanzen, resistent gegen den Anti-sens RNS aufweisenden Virus.
DE3751099T3 (de)	1986-04-02	2001-08-02	Pioneer Hi-Bred International, Inc.	Gegen Viren resistente Pflanzen mit einem viralen Überzugsprotein.
KR950012900B1 (ko) *	1992-08-19	1995-10-23	주식회사진로	항바이러스성 단백질 발현벡터와 이것으로 형질전환된 식물체의 제조방법

1986
- 1986-10-09 US US06/917,027 patent/US6608241B1/en not_active Expired - Lifetime
- 1986-10-23 CA CA000521247A patent/CA1341565C/en not_active Expired - Lifetime
- 1986-10-28 IE IE282886A patent/IE81098B1/en not_active IP Right Cessation
- 1986-10-28 IL IL8043386A patent/IL80433A/en not_active IP Right Cessation
- 1986-10-28 AU AU64528/86A patent/AU608204B2/en not_active Expired
- 1986-10-28 DK DK515186A patent/DK515186A/da not_active Application Discontinuation
- 1986-10-29 EP EP86308397A patent/EP0223452B1/de not_active Expired - Lifetime
- 1986-10-29 BG BG076908A patent/BG49720A3/xx unknown
- 1986-10-29 DE DE3650507T patent/DE3650507T2/de not_active Expired - Lifetime
- 1986-10-29 AT AT86308397T patent/ATE136330T1/de not_active IP Right Cessation
- 1986-10-29 ES ES86308397T patent/ES2087055T3/es not_active Expired - Lifetime
1994
- 1994-01-27 JP JP6007601A patent/JPH06339379A/ja active Pending
1995
- 1995-07-10 JP JP17377495A patent/JP3281512B2/ja not_active Expired - Fee Related
1996
- 1996-07-02 GR GR960401811T patent/GR3020438T3/el unknown
1999
- 1999-07-28 JP JP11214304A patent/JP2000041512A/ja active Pending
- 1999-11-19 JP JP11330565A patent/JP2000157288A/ja active Pending

Also Published As

Publication number	Publication date
EP0223452A2 (de)	1987-05-27
AU6452886A (en)	1987-04-30
EP0223452A3 (en)	1989-08-30
JP2000157288A (ja)	2000-06-13
IE862828L (en)	1987-04-29
EP0223452B1 (de)	1996-04-03
ATE136330T1 (de)	1996-04-15
IE81098B1 (en)	2000-03-08
CA1341565C (en)	2007-12-04
JPH06339379A (ja)	1994-12-13
BG49720A3 (bg)	1992-01-15
DE3650507D1 (de)	1996-05-09
GR3020438T3 (en)	1996-10-31
ES2087055T3 (es)	1996-07-16
DK515186A (da)	1987-04-30
JP3281512B2 (ja)	2002-05-13
DK515186D0 (da)	1986-10-28
AU608204B2 (en)	1991-03-28
JPH0851986A (ja)	1996-02-27
DE3650507T2 (de)	1996-10-17
US6608241B1 (en)	2003-08-19
JP2000041512A (ja)	2000-02-15

Legal Events

Date	Code	Title
1997-04-15	FF	Patent granted
1997-09-30	KB	Patent renewed
2001-04-30	KB	Patent renewed
2005-07-25	MM9K	Patent not in force due to non-payment of renewal fees

Publication	Publication Date	Title
CA1341565C (en)	2007-12-04	Protection of plants against viral infection
AU708035B2 (en)	1999-07-29	Transgenic plants expressing dna constructs containing a plurality of genes to impart virus resistance
Ghorbel et al.	2001	Transgenic citrus plants expressing the Citrus tristeza virus p23 protein exhibit viral‐like symptoms
US4970168A (en)	1990-11-13	Virus-resistant plants
Paplomatas et al.	1994	Molecular characterization of a new sap-transmissible bipartite genome geminivirus infecting tomatoes in Mexico
AU620039B2 (en)	1992-02-13	Inducible virus resistance in plants
CN1119422C (zh)	2003-08-27	抗病毒植物
Jacquet et al.	1998	High resistance to plum pox virus (PPV) in transgenic plants containing modified and truncated forms of PPV coat protein gene
AU707935B2 (en)	1999-07-22	Transgenic plants exhibiting heterologous virus resistance
US5185253A (en)	1993-02-09	Virus resistant plants
US20020059660A1 (en)	2002-05-16	Transgenic plants expressing DNA constructs containing a plurality of genes to impart virus resistance
KR100406666B1 (ko)	2004-12-23	토마토점무늬시들음병바이러스
AU692166B2 (en)	1998-06-04	Lettuce infectious yellows virus genes
JP2008283978A (ja)	2008-11-27	パパイヤ輪紋ウイルス遺伝子
Pałucha et al.	1998	An antisense coat protein gene confers immunity to potato leafroll virus in a genetically engineered potato
JPS62201527A (ja)	1987-09-05	ウイルス感染に対する植物保護
Erhardt et al.	1999	Transgenic plants expressing the TGB1 protein of peanut clump virus complement movement of TGB1-defective peanut clump virus but not of TGB1-defective beet necrotic yellow vein virus
NZ525957A (en)	2006-05-26	Method of enhancing virus-resistance in plants and producing virus-immune plants
EP0429497A1 (de)	1991-06-05	Cucumber-mosaikvirus-hüllproteingen.
EP0673423A4 (de)	1995-05-23	Virus resistenz hervorgerufen durch eine pflanzentransformation mit einem fragment einer replikase von einem pflanzen virusgenom.
US8901372B2 (en)	2014-12-02	Plant resistance to banana bunchy top virus
AU2002220347B2 (en)	2007-12-13	Method of enhancing virus-resistance in plants and producing virus-immune plants
IL104830A (en)	2001-01-28	A chimeric plasmid containing a non-structural gene for the protease of the potato virus and its various uses
PrimaryTransformants	0	Resistance to Tospoviruses in Nicotiana benthamiana Transformed with the N Gene of Tomato Spotted Wilt Virus: Correlation Between Transgene Expression and Protection In PrimaryTransformants