IL88751A - Chemical detectors that use catalytic antibodies - Google Patents
Chemical detectors that use catalytic antibodiesInfo
- Publication number
- IL88751A IL88751A IL8875188A IL8875188A IL88751A IL 88751 A IL88751 A IL 88751A IL 8875188 A IL8875188 A IL 8875188A IL 8875188 A IL8875188 A IL 8875188A IL 88751 A IL88751 A IL 88751A
- Authority
- IL
- Israel
- Prior art keywords
- antibody
- reaction
- analyte
- catalytic
- sensing
- Prior art date
Links
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- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0002—Antibodies with enzymatic activity, e.g. abzymes
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54366—Apparatus specially adapted for solid-phase testing
- G01N33/54373—Apparatus specially adapted for solid-phase testing involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
- G01N33/5438—Electrodes
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/544—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being organic
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Biochemistry (AREA)
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- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Organic Chemistry (AREA)
- General Engineering & Computer Science (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Investigating Or Analyzing Materials Using Thermal Means (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
Applications Claiming Priority (1)
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| US13854287A | 1987-12-24 | 1987-12-24 |
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| IL88751A0 IL88751A0 (en) | 1989-07-31 |
| IL88751A true IL88751A (en) | 1994-06-24 |
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| Application Number | Title | Priority Date | Filing Date |
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| IL8875188A IL88751A (en) | 1987-12-24 | 1988-12-21 | Chemical detectors that use catalytic antibodies |
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|---|---|
| EP (1) | EP0362304B1 (ja) |
| JP (1) | JP2662542B2 (ja) |
| KR (1) | KR0144002B1 (ja) |
| AT (1) | ATE149687T1 (ja) |
| AU (2) | AU2949689A (ja) |
| CA (1) | CA1308021C (ja) |
| DE (1) | DE3855814T2 (ja) |
| IL (1) | IL88751A (ja) |
| WO (1) | WO1989005977A1 (ja) |
| ZA (1) | ZA889546B (ja) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2017059337A1 (en) * | 2015-10-02 | 2017-04-06 | Georgetown University | High-resolution in situ electrochemical nmr with interdigitated electrodes |
Families Citing this family (47)
| Publication number | Priority date | Publication date | Assignee | Title |
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| DE3916432A1 (de) * | 1989-05-20 | 1990-11-22 | Cammann Karl | Verfahren und vorrichtung zur konzentrationsbestimmung eines antikoerper-antigenpaares |
| US5328847A (en) * | 1990-02-20 | 1994-07-12 | Case George D | Thin membrane sensor with biochemical switch |
| US5494831A (en) * | 1993-08-30 | 1996-02-27 | Hughes Aircraft Company | Electrochemical immunosensor system and methods |
| US5705399A (en) * | 1994-05-20 | 1998-01-06 | The Cooper Union For Advancement Of Science And Art | Sensor and method for detecting predetermined chemical species in solution |
| EP0821726B1 (en) * | 1995-03-10 | 2014-05-07 | Meso Scale Technologies, LLC. | Multi-array, multi-specific electrochemiluminescence testing |
| WO1996038726A1 (en) * | 1995-05-30 | 1996-12-05 | Ecole Polytechnique Federale De Lausanne (Epfl) | Covalently immobilized phospholipid bilayers on solid surfaces |
| CA2228970A1 (en) * | 1995-08-18 | 1997-02-27 | Donald W. Landry | Detection of organic compounds through regulation of antibody-catalyzed reactions |
| FR2746924B1 (fr) * | 1996-03-29 | 1998-06-05 | Indicia Diagnostics | Dispositif de detection d'une substance d'interet dans un fluide et procede d'utilisation |
| DE69721765T2 (de) * | 1996-06-20 | 2004-02-26 | New York University | Bestimmung von ligand-wechselwirkung mit einem polymermaterial |
| JP3612675B2 (ja) | 1997-06-05 | 2005-01-19 | 日本光電工業株式会社 | ｐＨ変化誘因性物質または生物体の測定装置及び測定方法 |
| DE19753056A1 (de) * | 1997-11-29 | 1999-08-05 | Wolfbeis Otto S Prof Dr | Künstliche Oberfläche mit vorbestimmter Affinität |
| GB9805014D0 (en) * | 1998-03-11 | 1998-05-06 | Siemens Plc | Improved mechanical arrangement for water quality measuring apparatus |
| EP0962773A1 (en) * | 1998-06-03 | 1999-12-08 | Mark Howard Jones | Electrochemical based assay processes instrument and labels |
| JP2001083162A (ja) * | 1999-09-17 | 2001-03-30 | Fuji Photo Film Co Ltd | 膜状生体高分子吸着材及び吸着用キット |
| JP4505776B2 (ja) * | 2001-01-19 | 2010-07-21 | 凸版印刷株式会社 | 遺伝子検出システム、これを備えた遺伝子検出装置、検出方法、並びに遺伝子検出用チップ |
| AU2002324947B2 (en) | 2001-09-10 | 2007-11-15 | Meso Scale Technologies, Llc. | Assay buffer, compositions containing the same, and methods of using the same |
| DE10163640A1 (de) * | 2001-12-21 | 2003-07-03 | Arno Kromminga | Nachweis von Wechselwirkungen zwischen mindestens zwei spezifischen Biomolekülen |
| JP3918622B2 (ja) * | 2002-04-23 | 2007-05-23 | アイシン精機株式会社 | 微小物質誘導装置 |
| DE10239290B3 (de) * | 2002-08-27 | 2004-04-08 | Dornier Medtech Systems Gmbh | Hochsensitiver Biosensor durch Aktivierung der erkennenden Komponente |
| JP2004245613A (ja) * | 2003-02-12 | 2004-09-02 | Japan Science & Technology Agency | フロ−セル型ｑｃｍ装置および試料測定方法 |
| GB0308324D0 (en) * | 2003-04-10 | 2003-05-14 | Piezoptic Ltd | A chemical sensing device |
| US7723099B2 (en) | 2003-09-10 | 2010-05-25 | Abbott Point Of Care Inc. | Immunoassay device with immuno-reference electrode |
| GB0609060D0 (en) * | 2006-05-08 | 2006-06-14 | Panopsys Ltd | A chemical sensing device |
| US8229535B2 (en) | 2008-02-21 | 2012-07-24 | Dexcom, Inc. | Systems and methods for blood glucose monitoring and alert delivery |
| US8682408B2 (en) | 2008-03-28 | 2014-03-25 | Dexcom, Inc. | Polymer membranes for continuous analyte sensors |
| US8583204B2 (en) | 2008-03-28 | 2013-11-12 | Dexcom, Inc. | Polymer membranes for continuous analyte sensors |
| US11730407B2 (en) | 2008-03-28 | 2023-08-22 | Dexcom, Inc. | Polymer membranes for continuous analyte sensors |
| WO2010134505A1 (ja) * | 2009-05-18 | 2010-11-25 | 日本電気株式会社 | 抗マラリア薬の免疫センサおよびその製造方法 |
| WO2010134506A1 (ja) * | 2009-05-18 | 2010-11-25 | 日本電気株式会社 | 爆発物検出用の免疫センサおよびその製造方法 |
| US9351677B2 (en) | 2009-07-02 | 2016-05-31 | Dexcom, Inc. | Analyte sensor with increased reference capacity |
| US20110027458A1 (en) | 2009-07-02 | 2011-02-03 | Dexcom, Inc. | Continuous analyte sensors and methods of making same |
| CN103502804B (zh) * | 2011-03-04 | 2015-07-22 | 加利福尼亚大学董事会 | 用于可逆的离子和分子感测或迁移的纳米孔装置 |
| RU2014125242A (ru) * | 2011-11-23 | 2015-12-27 | Дзе Гавернинг Каунсил Оф Дзе Юниверсити Оф Торонто | Универсальный и чувствительный биодатчик |
| CN102636463A (zh) * | 2012-04-24 | 2012-08-15 | 攀钢集团攀枝花钢铁研究院有限公司 | 硫酸氧钛水解的在线检测装置及控制设备 |
| KR101420916B1 (ko) * | 2012-05-22 | 2014-07-17 | 한양대학교 산학협력단 | 세포 계수 장치 및 세포 계수 방법 |
| CN105431853B (zh) * | 2013-05-23 | 2018-12-28 | 艾弗诺泰普有限责任公司 | 表型整合的社会研究数据库和方法 |
| US9341639B2 (en) | 2013-07-26 | 2016-05-17 | Industrial Technology Research Institute | Apparatus for microfluid detection |
| WO2016040826A1 (en) | 2014-09-12 | 2016-03-17 | Nipro Diagnostics, Inc. | Apparatus for diagnostic meter strip control and identification |
| WO2017123781A1 (en) * | 2016-01-12 | 2017-07-20 | Ambergen, Inc. | Proteome-wide drug screening using mass spectrometric imaging (msi) of bead-arrays |
| CN106290469B (zh) * | 2016-09-29 | 2023-06-20 | 中国石油大学(华东) | 一种油气井燃爆压裂药剂高温高压安全性测试装置及其使用方法 |
| KR20190072520A (ko) | 2016-10-24 | 2019-06-25 | 도레이 카부시키가이샤 | 반도체 센서 및 그의 제조 방법, 그리고 복합 센서 |
| JP7017727B2 (ja) * | 2017-06-05 | 2022-02-09 | 島根県 | 味覚センサ |
| JP2019039734A (ja) * | 2017-08-24 | 2019-03-14 | 学校法人東北学院 | イオン・バイオセンサチップとイオン・バイオセンサモジュールおよびこれらを用いたイオン・バイオセンサ |
| CN107884392B (zh) * | 2017-10-24 | 2020-09-18 | 中国航天空气动力技术研究院 | 电弧加热器气流焓值光谱测量系统 |
| CN113348526B (zh) | 2019-02-05 | 2023-03-14 | 日立金属株式会社 | 卷绕磁芯、合金芯以及卷绕磁芯的制造方法 |
| JP2021117172A (ja) * | 2020-01-29 | 2021-08-10 | 三菱マテリアル株式会社 | バイオセンサ |
| EP4356132B1 (en) * | 2021-06-17 | 2025-10-01 | F. Hoffmann-La Roche AG | Method for immunosensing on a lipid layer |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU3745285A (en) * | 1983-11-29 | 1985-06-13 | Igen, Inc. | Method of catalyzing chemical reactions |
| US4659567A (en) * | 1984-09-07 | 1987-04-21 | Scripps Clinic & Research Foundation | Molecules with antibody combining sites that bind to hydrolytic transition states |
| ZA893106B (en) * | 1988-04-29 | 1990-01-31 | Igen Inc | Interaction system comprising a surfactant-stabilized disperse aqueous phase containing an antibody or antibody fragment |
-
1988
- 1988-12-02 ZA ZA889546A patent/ZA889546B/xx unknown
- 1988-12-12 EP EP89901650A patent/EP0362304B1/en not_active Expired - Lifetime
- 1988-12-12 AT AT89901650T patent/ATE149687T1/de not_active IP Right Cessation
- 1988-12-12 AU AU29496/89A patent/AU2949689A/en not_active Abandoned
- 1988-12-12 DE DE3855814T patent/DE3855814T2/de not_active Expired - Fee Related
- 1988-12-12 WO PCT/US1988/004426 patent/WO1989005977A1/en not_active Ceased
- 1988-12-12 KR KR1019890701599A patent/KR0144002B1/ko not_active Expired - Fee Related
- 1988-12-12 JP JP1501577A patent/JP2662542B2/ja not_active Expired - Fee Related
- 1988-12-21 CA CA000586571A patent/CA1308021C/en not_active Expired - Lifetime
- 1988-12-21 IL IL8875188A patent/IL88751A/en unknown
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1994
- 1994-01-10 AU AU53110/94A patent/AU669758B2/en not_active Ceased
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2017059337A1 (en) * | 2015-10-02 | 2017-04-06 | Georgetown University | High-resolution in situ electrochemical nmr with interdigitated electrodes |
Also Published As
| Publication number | Publication date |
|---|---|
| ATE149687T1 (de) | 1997-03-15 |
| ZA889546B (en) | 1990-08-29 |
| IL88751A0 (en) | 1989-07-31 |
| EP0362304A4 (en) | 1991-04-10 |
| DE3855814T2 (de) | 1997-10-02 |
| AU669758B2 (en) | 1996-06-20 |
| WO1989005977A1 (en) | 1989-06-29 |
| EP0362304A1 (en) | 1990-04-11 |
| KR900700882A (ko) | 1990-08-17 |
| JP2662542B2 (ja) | 1997-10-15 |
| CA1308021C (en) | 1992-09-29 |
| KR0144002B1 (ko) | 1998-07-15 |
| JPH02501860A (ja) | 1990-06-21 |
| AU2949689A (en) | 1989-07-19 |
| DE3855814D1 (de) | 1997-04-10 |
| AU5311094A (en) | 1994-03-10 |
| EP0362304B1 (en) | 1997-03-05 |
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