IL89814A - Regulation of gene expression - Google Patents

Regulation of gene expression

Info

Publication number: IL89814A
Authority: IL; Israel
Prior art keywords: protein; dna; gene; binding; heterodimer
Prior art date: 1988-03-31

Application number

IL8981489A

Other languages

English (en)

Hebrew (he)

Other versions

IL89814A0 (en

Original Assignee

Ici Plc

Harvard College

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1988-03-31

Filing date

1989-03-31

Publication date

1995-06-29

1989-03-31 Application filed by Ici Plc, Harvard College filed Critical Ici Plc

1989-09-28 Publication of IL89814A0 publication Critical patent/IL89814A0/xx

1995-06-29 Publication of IL89814A publication Critical patent/IL89814A/en

Links

230000014493 regulation of gene expression Effects 0.000 title description 5
108090000623 proteins and genes Proteins 0.000 claims abstract description 194
102000004169 proteins and genes Human genes 0.000 claims abstract description 129
238000009739 binding Methods 0.000 claims abstract description 76
230000027455 binding Effects 0.000 claims abstract description 75
239000000833 heterodimer Substances 0.000 claims abstract description 44
230000001105 regulatory effect Effects 0.000 claims abstract description 37
230000014509 gene expression Effects 0.000 claims abstract description 31
108091028043 Nucleic acid sequence Proteins 0.000 claims description 54
230000004568 DNA-binding Effects 0.000 claims description 40
239000000178 monomer Substances 0.000 claims description 40
230000000295 complement effect Effects 0.000 claims description 16
238000004519 manufacturing process Methods 0.000 claims description 11
238000000034 method Methods 0.000 claims description 11
230000004044 response Effects 0.000 claims description 8
108091006104 gene-regulatory proteins Proteins 0.000 claims description 7
102000034356 gene-regulatory proteins Human genes 0.000 claims description 7
239000002773 nucleotide Substances 0.000 claims description 4
125000003729 nucleotide group Chemical group 0.000 claims description 4
230000002401 inhibitory effect Effects 0.000 claims description 2
230000001737 promoting effect Effects 0.000 claims 1
108020004414 DNA Proteins 0.000 description 26
102220011397 rs267607538 Human genes 0.000 description 11
239000000539 dimer Substances 0.000 description 10
238000002474 experimental method Methods 0.000 description 9
239000013612 plasmid Substances 0.000 description 8
239000000710 homodimer Substances 0.000 description 7
239000012634 fragment Substances 0.000 description 6
239000000203 mixture Substances 0.000 description 6
108010034634 Repressor Proteins Proteins 0.000 description 5
102000009661 Repressor Proteins Human genes 0.000 description 5
230000033228 biological regulation Effects 0.000 description 5
102000053602 DNA Human genes 0.000 description 4
238000002360 preparation method Methods 0.000 description 4
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150000001413 amino acids Chemical group 0.000 description 3
230000008901 benefit Effects 0.000 description 3
230000001419 dependent effect Effects 0.000 description 3
229920001220 nitrocellulos Polymers 0.000 description 3
239000002904 solvent Substances 0.000 description 3
102000052510 DNA-Binding Proteins Human genes 0.000 description 2
102000004163 DNA-directed RNA polymerases Human genes 0.000 description 2
108090000626 DNA-directed RNA polymerases Proteins 0.000 description 2
102100039556 Galectin-4 Human genes 0.000 description 2
101000608765 Homo sapiens Galectin-4 Proteins 0.000 description 2
101000740205 Homo sapiens Sal-like protein 1 Proteins 0.000 description 2
TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 2
WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 description 2
240000004808 Saccharomyces cerevisiae Species 0.000 description 2
102100037204 Sal-like protein 1 Human genes 0.000 description 2
230000001580 bacterial effect Effects 0.000 description 2
230000015572 biosynthetic process Effects 0.000 description 2
239000008280 blood Substances 0.000 description 2
210000004369 blood Anatomy 0.000 description 2
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102000014914 Carrier Proteins Human genes 0.000 description 1
108700020911 DNA-Binding Proteins Proteins 0.000 description 1
101710096438 DNA-binding protein Proteins 0.000 description 1
239000003109 Disodium ethylene diamine tetraacetate Substances 0.000 description 1
ZGTMUACCHSMWAC-UHFFFAOYSA-L EDTA disodium salt (anhydrous) Chemical compound [Na+].[Na+].OC(=O)CN(CC([O-])=O)CCN(CC(O)=O)CC([O-])=O ZGTMUACCHSMWAC-UHFFFAOYSA-L 0.000 description 1
241000588724 Escherichia coli Species 0.000 description 1
VGGSQFUCUMXWEO-UHFFFAOYSA-N Ethene Chemical compound C=C VGGSQFUCUMXWEO-UHFFFAOYSA-N 0.000 description 1
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101000895481 Homo sapiens Corticoliberin Proteins 0.000 description 1
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102000008422 Polynucleotide 5'-hydroxyl-kinase Human genes 0.000 description 1
108020004511 Recombinant DNA Proteins 0.000 description 1
JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 1
230000004075 alteration Effects 0.000 description 1
238000013459 approach Methods 0.000 description 1
238000003556 assay Methods 0.000 description 1
230000010455 autoregulation Effects 0.000 description 1
108091008324 binding proteins Proteins 0.000 description 1
238000006243 chemical reaction Methods 0.000 description 1
238000010367 cloning Methods 0.000 description 1
AGVAZMGAQJOSFJ-WZHZPDAFSA-M cobalt(2+);[(2r,3s,4r,5s)-5-(5,6-dimethylbenzimidazol-1-yl)-4-hydroxy-2-(hydroxymethyl)oxolan-3-yl] [(2r)-1-[3-[(1r,2r,3r,4z,7s,9z,12s,13s,14z,17s,18s,19r)-2,13,18-tris(2-amino-2-oxoethyl)-7,12,17-tris(3-amino-3-oxopropyl)-3,5,8,8,13,15,18,19-octamethyl-2 Chemical compound [Co+2].N#[C-].[N-]([C@@H]1[C@H](CC(N)=O)[C@@]2(C)CCC(=O)NC[C@@H](C)OP(O)(=O)O[C@H]3[C@H]([C@H](O[C@@H]3CO)N3C4=CC(C)=C(C)C=C4N=C3)O)\C2=C(C)/C([C@H](C\2(C)C)CCC(N)=O)=N/C/2=C\C([C@H]([C@@]/2(CC(N)=O)C)CCC(N)=O)=N\C\2=C(C)/C2=N[C@]1(C)[C@@](C)(CC(N)=O)[C@@H]2CCC(N)=O AGVAZMGAQJOSFJ-WZHZPDAFSA-M 0.000 description 1
230000001276 controlling effect Effects 0.000 description 1
238000012217 deletion Methods 0.000 description 1
230000037430 deletion Effects 0.000 description 1
235000019301 disodium ethylene diamine tetraacetate Nutrition 0.000 description 1
238000001962 electrophoresis Methods 0.000 description 1
230000009881 electrostatic interaction Effects 0.000 description 1
230000004927 fusion Effects 0.000 description 1
238000010353 genetic engineering Methods 0.000 description 1
238000000338 in vitro Methods 0.000 description 1
230000005764 inhibitory process Effects 0.000 description 1
230000003993 interaction Effects 0.000 description 1
238000005304 joining Methods 0.000 description 1
229910001629 magnesium chloride Inorganic materials 0.000 description 1
230000007246 mechanism Effects 0.000 description 1
108020004999 messenger RNA Proteins 0.000 description 1
238000002156 mixing Methods 0.000 description 1
238000002703 mutagenesis Methods 0.000 description 1
231100000350 mutagenesis Toxicity 0.000 description 1
230000004962 physiological condition Effects 0.000 description 1
239000001103 potassium chloride Substances 0.000 description 1
235000011164 potassium chloride Nutrition 0.000 description 1
238000011533 pre-incubation Methods 0.000 description 1
239000013636 protein dimer Substances 0.000 description 1
238000001742 protein purification Methods 0.000 description 1
150000003212 purines Chemical class 0.000 description 1
238000003345 scintillation counting Methods 0.000 description 1
230000007017 scission Effects 0.000 description 1
238000012163 sequencing technique Methods 0.000 description 1
238000013207 serial dilution Methods 0.000 description 1
150000003384 small molecules Chemical class 0.000 description 1
IHQKEDIOMGYHEB-UHFFFAOYSA-M sodium dimethylarsinate Chemical compound [Na+].C[As](C)([O-])=O IHQKEDIOMGYHEB-UHFFFAOYSA-M 0.000 description 1
230000009870 specific binding Effects 0.000 description 1
230000004936 stimulating effect Effects 0.000 description 1
238000013518 transcription Methods 0.000 description 1
230000035897 transcription Effects 0.000 description 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/67—General methods for enhancing the expression

Landscapes

Genetics & Genomics (AREA)
Health & Medical Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Engineering & Computer Science (AREA)
Wood Science & Technology (AREA)
Organic Chemistry (AREA)
Biomedical Technology (AREA)
Biotechnology (AREA)
General Engineering & Computer Science (AREA)
Chemical & Material Sciences (AREA)
Zoology (AREA)
Bioinformatics & Cheminformatics (AREA)
Biophysics (AREA)
Plant Pathology (AREA)
Molecular Biology (AREA)
Biochemistry (AREA)
General Health & Medical Sciences (AREA)
Physics & Mathematics (AREA)
Microbiology (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)
Peptides Or Proteins (AREA)
Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)
Control Of Eletrric Generators (AREA)
Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Saccharide Compounds (AREA)

IL8981489A 1988-03-31 1989-03-31 Regulation of gene expression IL89814A (en)

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
GB888807683A GB8807683D0 (en)	1988-03-31	1988-03-31	Regulation of gene expression

Publications (2)

Publication Number	Publication Date
IL89814A0 IL89814A0 (en)	1989-09-28
IL89814A true IL89814A (en)	1995-06-29

Family

ID=10634427

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
IL8981489A IL89814A (en)	1988-03-31	1989-03-31	Regulation of gene expression

Country Status (14)

Country	Link
US (1)	US5554510A (de)
EP (1)	EP0338690B1 (de)
JP (1)	JP2991731B2 (de)
AT (1)	ATE141102T1 (de)
AU (1)	AU629208B2 (de)
CA (1)	CA1338620C (de)
DE (1)	DE68926918T2 (de)
DK (1)	DK234790A (de)
GB (1)	GB8807683D0 (de)
HU (1)	HUT55051A (de)
IL (1)	IL89814A (de)
NZ (1)	NZ228568A (de)
WO (1)	WO1989009256A1 (de)
ZA (1)	ZA892401B (de)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
EP0945515A3 (de) *	1989-11-06	2002-08-21	Cell Genesys, Inc.	Herstellung von Proteinen mittels homologer Rekombination
ES2151463T5 (es) *	1989-12-22	2012-10-29	Merck Serono Sa	Constructos de ADN para la activación y la modificación de la expresión de genes endógenos
US5272071A (en) *	1989-12-22	1993-12-21	Applied Research Systems Ars Holding N.V.	Method for the modification of the expression characteristics of an endogenous gene of a given cell line
US5521066A (en) *	1993-09-13	1996-05-28	Bristol-Myers Squibb Company	Periplasmic membrane-bound system for detecting protein-protein interactions
CA2336207C (en)	1998-09-10	2004-03-23	Pioneer Hi-Bred International, Inc.	Ecdysone receptors and methods for their use
US7803982B2 (en)	2001-04-20	2010-09-28	The Mount Sinai School Of Medicine Of New York University	T1R3 transgenic animals, cells and related methods

1988
- 1988-03-31 GB GB888807683A patent/GB8807683D0/en active Pending
1989
- 1989-03-31 ZA ZA892401A patent/ZA892401B/xx unknown
- 1989-03-31 NZ NZ228568A patent/NZ228568A/xx unknown
- 1989-03-31 JP JP1504171A patent/JP2991731B2/ja not_active Expired - Lifetime
- 1989-03-31 WO PCT/GB1989/000334 patent/WO1989009256A1/en not_active Ceased
- 1989-03-31 HU HU892210A patent/HUT55051A/hu unknown
- 1989-03-31 AU AU34316/89A patent/AU629208B2/en not_active Expired
- 1989-03-31 CA CA000595442A patent/CA1338620C/en not_active Expired - Lifetime
- 1989-03-31 EP EP89303213A patent/EP0338690B1/de not_active Expired - Lifetime
- 1989-03-31 AT AT89303213T patent/ATE141102T1/de not_active IP Right Cessation
- 1989-03-31 DE DE68926918T patent/DE68926918T2/de not_active Expired - Lifetime
- 1989-03-31 IL IL8981489A patent/IL89814A/en not_active IP Right Cessation
1990
- 1990-09-28 DK DK234790A patent/DK234790A/da not_active Application Discontinuation
1993
- 1993-12-01 US US08/161,170 patent/US5554510A/en not_active Expired - Lifetime

Also Published As

Publication number	Publication date
EP0338690B1 (de)	1996-08-07
DK234790D0 (da)	1990-09-28
CA1338620C (en)	1996-10-01
IL89814A0 (en)	1989-09-28
DE68926918D1 (de)	1996-09-12
HUT55051A (en)	1991-04-29
AU629208B2 (en)	1992-10-01
JP2991731B2 (ja)	1999-12-20
DE68926918T2 (de)	1996-11-28
DK234790A (da)	1990-09-28
ATE141102T1 (de)	1996-08-15
EP0338690A1 (de)	1989-10-25
GB8807683D0 (en)	1988-05-05
AU3431689A (en)	1989-10-16
NZ228568A (en)	1992-10-28
JPH03503601A (ja)	1991-08-15
HU892210D0 (en)	1991-02-28
ZA892401B (en)	1989-12-27
US5554510A (en)	1996-09-10
WO1989009256A1 (en)	1989-10-05

Legal Events

Date	Code	Title	Description
2000-02-17	RH	Patent void

Publication	Publication Date	Title
US5464758A (en)	1995-11-07	Tight control of gene expression in eucaryotic cells by tetracycline-responsive promoters
Gossen et al.	1992	Tight control of gene expression in mammalian cells by tetracycline-responsive promoters.
Giffin et al.	1996	Sequence-specific DNA binding by Ku autoantigen and its effects on transcription
US8501454B2 (en)	2013-08-06	Homologous recombination-based DNA cloning compositions
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JP7418796B2 (ja)	2024-01-22	細胞株の開発のための相同組換えベクターの高速生成のためのｄｎａプラスミド
JP4528623B2 (ja)	2010-08-18	迅速分解性レポーター融合タンパク質
US20090176281A1 (en)	2009-07-09	Modular vector systems
EP0338690B1 (de)	1996-08-07	Regulierung der Genexpression
US6150511A (en)	2000-11-21	Chimeric enzyme for promoting targeted integration of foreign DNA into a host genome
US20250340866A1 (en)	2025-11-06	A screening method
JPH029368A (ja)	1990-01-12	発現ベクター
KR20220008274A (ko)	2022-01-20	안정한 표적화 통합
CN102296087B (zh)	2012-11-28	基于枯草芽孢杆菌生物素连接酶的外源基因诱导表达调控系统及其构建方法
Cao et al.	1993	Expression and functional analysis of steroid receptor fragments secreted from Staphylococcus aureus
CA2224475A1 (en)	1999-08-25	Protein interaction and transcription factor trap
JP2025531410A (ja)	2025-09-19	インテグレーション及び選択システム
JPS61242583A (ja)	1986-10-28	ｔｒｐオペロンのシヤイン‐ダルガーノ配列と開始コードンとの間のＤＮＡ配列におけるタンパク質発現を増大させるための改変
Michiels et al.	0	Features of the 14 bp promoter element regulating Drosophila β 2 tubulin gene expression in male germ cells
Sengupta	2000	Analysis of Yeast 2um Plasmid Segregation
CA2320894A1 (en)	1999-09-02	Protein interaction and transcription factor trap