IL92265A - Increases expression and its application in increasing the utilization of recombinant gene bioavailability - Google Patents

Increases expression and its application in increasing the utilization of recombinant gene bioavailability

Info

Publication number: IL92265A
Authority: IL; Israel
Prior art keywords: expression; enhancer; sequence; gene; plasmid
Prior art date: 1988-11-11

Application number

IL9226589A

Other languages

English (en)

Hebrew (he)

Other versions

IL92265A0 (en

Original Assignee

Boehringer Mannheim Gmbh

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1988-11-11

Filing date

1989-11-09

Publication date

1995-08-31

1989-11-09 Application filed by Boehringer Mannheim Gmbh filed Critical Boehringer Mannheim Gmbh

1990-07-26 Publication of IL92265A0 publication Critical patent/IL92265A0/xx

1995-08-31 Publication of IL92265A publication Critical patent/IL92265A/en

Links

108090000623 proteins and genes Proteins 0.000 title claims abstract description 48
239000003623 enhancer Substances 0.000 title claims abstract description 40
239000013604 expression vector Substances 0.000 claims abstract description 14
108091034117 Oligonucleotide Proteins 0.000 claims abstract description 13
108020004414 DNA Proteins 0.000 claims abstract description 12
230000000295 complement effect Effects 0.000 claims abstract description 9
238000013518 transcription Methods 0.000 claims abstract description 6
230000035897 transcription Effects 0.000 claims abstract description 6
108020005098 Anticodon Proteins 0.000 claims abstract description 5
108020004566 Transfer RNA Proteins 0.000 claims abstract description 5
108091028043 Nucleic acid sequence Proteins 0.000 claims abstract description 4
230000009466 transformation Effects 0.000 claims abstract 2
239000013612 plasmid Substances 0.000 claims description 31
241000588724 Escherichia coli Species 0.000 claims description 18
239000013598 vector Substances 0.000 claims description 17
239000013613 expression plasmid Substances 0.000 claims description 11
238000000034 method Methods 0.000 claims description 7
230000002759 chromosomal effect Effects 0.000 claims description 4
230000001939 inductive effect Effects 0.000 claims description 4
238000009396 hybridization Methods 0.000 claims description 3
238000004519 manufacturing process Methods 0.000 claims description 3
108090000435 Urokinase-type plasminogen activator Proteins 0.000 claims description 2
102000003990 Urokinase-type plasminogen activator Human genes 0.000 claims description 2
229960005356 urokinase Drugs 0.000 claims description 2
108050006955 Tissue-type plasminogen activator Proteins 0.000 description 11
102100033571 Tissue-type plasminogen activator Human genes 0.000 description 11
239000012634 fragment Substances 0.000 description 7
230000006698 induction Effects 0.000 description 5
108091032973 (ribonucleotides)n+m Proteins 0.000 description 3
BPHPUYQFMNQIOC-NXRLNHOXSA-N isopropyl beta-D-thiogalactopyranoside Chemical compound CC(C)S[C@@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O BPHPUYQFMNQIOC-NXRLNHOXSA-N 0.000 description 3
108091008146 restriction endonucleases Proteins 0.000 description 3
229930185560 Pseudouridine Natural products 0.000 description 2
PTJWIQPHWPFNBW-UHFFFAOYSA-N Pseudouridine C Natural products OC1C(O)C(CO)OC1C1=CNC(=O)NC1=O PTJWIQPHWPFNBW-UHFFFAOYSA-N 0.000 description 2
229960000723 ampicillin Drugs 0.000 description 2
AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 description 2
101150008083 ant gene Proteins 0.000 description 2
WGDUUQDYDIIBKT-UHFFFAOYSA-N beta-Pseudouridine Natural products OC1OC(CN2C=CC(=O)NC2=O)C(O)C1O WGDUUQDYDIIBKT-UHFFFAOYSA-N 0.000 description 2
230000000694 effects Effects 0.000 description 2
PTJWIQPHWPFNBW-GBNDHIKLSA-N pseudouridine Chemical compound O[C@@H]1[C@H](O)[C@@H](CO)O[C@H]1C1=CNC(=O)NC1=O PTJWIQPHWPFNBW-GBNDHIKLSA-N 0.000 description 2
230000010076 replication Effects 0.000 description 2
108091028026 C-DNA Proteins 0.000 description 1
102000004190 Enzymes Human genes 0.000 description 1
108090000790 Enzymes Proteins 0.000 description 1
101710163270 Nuclease Proteins 0.000 description 1
108700005078 Synthetic Genes Proteins 0.000 description 1
238000004458 analytical method Methods 0.000 description 1
238000010170 biological method Methods 0.000 description 1
238000003776 cleavage reaction Methods 0.000 description 1
238000010367 cloning Methods 0.000 description 1
239000002299 complementary DNA Substances 0.000 description 1
238000010276 construction Methods 0.000 description 1
238000012217 deletion Methods 0.000 description 1
230000037430 deletion Effects 0.000 description 1
230000029087 digestion Effects 0.000 description 1
ZPTBLXKRQACLCR-XVFCMESISA-N dihydrouridine Chemical compound O[C@@H]1[C@H](O)[C@@H](CO)O[C@H]1N1C(=O)NC(=O)CC1 ZPTBLXKRQACLCR-XVFCMESISA-N 0.000 description 1
238000010828 elution Methods 0.000 description 1
229940088598 enzyme Drugs 0.000 description 1
150000002170 ethers Chemical class 0.000 description 1
238000000855 fermentation Methods 0.000 description 1
230000004151 fermentation Effects 0.000 description 1
238000001502 gel electrophoresis Methods 0.000 description 1
-1 isopropyl- Chemical group 0.000 description 1
229960000318 kanamycin Drugs 0.000 description 1
102000004169 proteins and genes Human genes 0.000 description 1
230000007017 scission Effects 0.000 description 1
230000035945 sensitivity Effects 0.000 description 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6424—Serine endopeptidases (3.4.21)
- C12N9/6456—Plasminogen activators
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/67—General methods for enhancing the expression
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli

Landscapes

Health & Medical Sciences (AREA)
Genetics & Genomics (AREA)
Engineering & Computer Science (AREA)
Life Sciences & Earth Sciences (AREA)
Chemical & Material Sciences (AREA)
Zoology (AREA)
Biomedical Technology (AREA)
Organic Chemistry (AREA)
Bioinformatics & Cheminformatics (AREA)
Wood Science & Technology (AREA)
Biotechnology (AREA)
General Engineering & Computer Science (AREA)
Molecular Biology (AREA)
Biochemistry (AREA)
Microbiology (AREA)
General Health & Medical Sciences (AREA)
Physics & Mathematics (AREA)
Biophysics (AREA)
Plant Pathology (AREA)
Medicinal Chemistry (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)
Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Enzymes And Modification Thereof (AREA)
Saccharide Compounds (AREA)

IL9226589A 1988-11-11 1989-11-09 Increases expression and its application in increasing the utilization of recombinant gene bioavailability IL92265A (en)

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
DE3838377A DE3838377A1 (de)	1988-11-11	1988-11-11	Expressions-enhancer und dessen verwendung zur erhoehung der ausbeute bei der expression von rekombinanten genen

Publications (2)

Publication Number	Publication Date
IL92265A0 IL92265A0 (en)	1990-07-26
IL92265A true IL92265A (en)	1995-08-31

Family

ID=6367027

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
IL9226589A IL92265A (en)	1988-11-11	1989-11-09	Increases expression and its application in increasing the utilization of recombinant gene bioavailability

Country Status (10)

Country	Link
US (1)	US5336602A (fr)
EP (1)	EP0368342B1 (fr)
JP (1)	JP2667261B2 (fr)
AT (1)	ATE111527T1 (fr)
AU (1)	AU608180B2 (fr)
CA (1)	CA2002515C (fr)
DE (2)	DE3838377A1 (fr)
DK (1)	DK175757B1 (fr)
ES (1)	ES2060725T3 (fr)
IL (1)	IL92265A (fr)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
AU2660397A (en) *	1996-04-05	1997-10-29	Board Of Regents, The University Of Texas System	Methods for producing soluble, biologically-active disulfide bond-containing eukaryotic proteins in bacterial cells
US6083715A (en) *	1997-06-09	2000-07-04	Board Of Regents, The University Of Texas System	Methods for producing heterologous disulfide bond-containing polypeptides in bacterial cells
KR101056983B1 (ko) *	2003-07-11	2011-08-16	가부시키가이샤 구라레	적층 시트와 그 설계방법 및 배면 투사형 스크린과 그제조방법

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US4654307A (en) *	1983-02-17	1987-03-31	The Research Foundation Of State University Of New York	Novel bacteria containing a plasmid having a tRNA code
AU618640B2 (en) *	1988-11-11	1992-01-02	Boehringer Mannheim Gmbh	Process for the expression of a recombinant gene

1988
- 1988-11-11 DE DE3838377A patent/DE3838377A1/de not_active Withdrawn
1989
- 1989-11-06 AU AU44445/89A patent/AU608180B2/en not_active Ceased
- 1989-11-08 CA CA002002515A patent/CA2002515C/fr not_active Expired - Lifetime
- 1989-11-09 DK DK198905618A patent/DK175757B1/da not_active IP Right Cessation
- 1989-11-09 IL IL9226589A patent/IL92265A/en unknown
- 1989-11-10 ES ES89120894T patent/ES2060725T3/es not_active Expired - Lifetime
- 1989-11-10 AT AT89120894T patent/ATE111527T1/de not_active IP Right Cessation
- 1989-11-10 EP EP89120894A patent/EP0368342B1/fr not_active Expired - Lifetime
- 1989-11-10 DE DE58908358T patent/DE58908358D1/de not_active Expired - Lifetime
- 1989-11-13 JP JP1292538A patent/JP2667261B2/ja not_active Expired - Lifetime
1993
- 1993-01-22 US US08/007,405 patent/US5336602A/en not_active Expired - Lifetime

Also Published As

Publication number	Publication date
US5336602A (en)	1994-08-09
AU4444589A (en)	1990-05-17
AU608180B2 (en)	1991-03-21
CA2002515A1 (fr)	1990-05-11
ATE111527T1 (de)	1994-09-15
JPH02182191A (ja)	1990-07-16
ES2060725T3 (es)	1994-12-01
CA2002515C (fr)	1996-03-19
DK175757B1 (da)	2005-02-14
DK561889A (da)	1990-05-12
EP0368342A3 (fr)	1991-10-16
DE3838377A1 (de)	1990-05-23
DE58908358D1 (de)	1994-10-20
IL92265A0 (en)	1990-07-26
EP0368342A2 (fr)	1990-05-16
JP2667261B2 (ja)	1997-10-27
EP0368342B1 (fr)	1994-09-14
DK561889D0 (da)	1989-11-09

Legal Events

Date	Code	Title
1996-03-31	KB	Patent renewed
2000-07-26	KB	Patent renewed
2000-08-31	KB	Patent renewed
2004-01-04	KB	Patent renewed
2008-01-06	KB	Patent renewed

Publication	Publication Date	Title
NO840200L (no)	1984-07-30	Glukoamylase cdna.
AU618640B2 (en)	1992-01-02	Process for the expression of a recombinant gene
Botterman et al.	1985	High-level production of the EcoRI endonuclease under the control of the pL promoter of bacterio-phage lambda
JP2544602B2 (ja)	1996-10-16	新規プラスミドベクタ−
EP0077196A2 (fr)	1983-04-20	Microorganismes produisant des acides aminés aromatiques
EP0281391A1 (fr)	1988-09-07	Vecteurs recombinants d'expression d'ADN et composés d'ADN codant pour la déacétoxycéphalosporine C synthétase et la déacétylcéphalosporine C synthétase
US5637503A (en)	1997-06-10	Plasmids, their construction and their use in the manufacture of a plasminogen activator
US4710464A (en)	1987-12-01	Transcription terminators
IL92265A (en)	1995-08-31	Increases expression and its application in increasing the utilization of recombinant gene bioavailability
JPH0452760B2 (fr)	1992-08-24
US4963482A (en)	1990-10-16	DNA for the repressible and inducible expression of foreign genes
EP0154272A2 (fr)	1985-09-11	Production d'urokinase humaine
US4885252A (en)	1989-12-05	Recombinant DNA expression vectors and DNA compounds that encode isopenicillin N synthetase from aspergillus nidulans
NO179046B (no)	1996-04-15	Fremgangsmåte for fremstilling av biologisk aktivt human enkelt kjede urokinase-type plasminogenaktivator
CA2622710C (fr)	2016-10-18	Origine transferable hybride de plasmides a replication
US4631257A (en)	1986-12-23	Stable high copy number plasmids
Minkley Jr et al.	1984	Escherichia coli DNA polymerase I. Construction of a polA plasmid for amplification and an improved purification scheme.
EP0374162B1 (fr)	1995-03-29	Systeme eqilibre de transcription constitutive par induction
CA2121481C (fr)	2006-08-08	Expression de polypeptides dans e.coli sous le controle du promoteur du gene mdh de e.coli
Foltermann et al.	1984	Assembly of the aspartate transcarbamoylase holoenzyme from transcriptionally independent catalytic and regulatory cistrons
EP0141362A2 (fr)	1985-05-15	Nouveau vecteur de plasmide
US4529695A (en)	1985-07-16	Recombinant cloning vector, host for the vector, and method for using same
US4476227A (en)	1984-10-09	Cosmid cloning vectors
US4966840A (en)	1990-10-30	Stable high copy number plasmids
US5576190A (en)	1996-11-19	Bacteriophage Lambda pL promoters