IL94724A - Decomposition of red blood cells and denaturation of hemoglobin by the use of lithium salts - Google Patents

Decomposition of red blood cells and denaturation of hemoglobin by the use of lithium salts

Info

Publication number: IL94724A
Authority: IL; Israel
Prior art keywords: hemoglobin; derivative; lysing; lithium; amount
Prior art date: 1989-07-13

Application number

IL9472490A

Other languages

English (en)

Hebrew (he)

Other versions

IL94724A0 (en

Original Assignee

Miles Inc

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1989-07-13

Filing date

1990-06-13

Publication date

1994-02-27

1990-06-13 Application filed by Miles Inc filed Critical Miles Inc

1991-04-15 Publication of IL94724A0 publication Critical patent/IL94724A0/xx

1994-02-27 Publication of IL94724A publication Critical patent/IL94724A/en

Links

108010054147 Hemoglobins Proteins 0.000 title claims abstract description 114
102000001554 Hemoglobins Human genes 0.000 title claims abstract description 114
210000003743 erythrocyte Anatomy 0.000 title claims abstract description 38
230000009089 cytolysis Effects 0.000 title claims abstract description 36
159000000002 lithium salts Chemical class 0.000 title claims abstract description 24
229910003002 lithium salt Inorganic materials 0.000 title claims abstract description 17
210000004369 blood Anatomy 0.000 claims abstract description 40
239000008280 blood Substances 0.000 claims abstract description 40
238000003018 immunoassay Methods 0.000 claims abstract description 37
238000000034 method Methods 0.000 claims abstract description 36
230000002934 lysing effect Effects 0.000 claims abstract description 34
238000004925 denaturation Methods 0.000 claims abstract description 30
230000036425 denaturation Effects 0.000 claims abstract description 30
ZJZXSOKJEJFHCP-UHFFFAOYSA-M lithium;thiocyanate Chemical compound [Li+].[S-]C#N ZJZXSOKJEJFHCP-UHFFFAOYSA-M 0.000 claims abstract description 21
239000003153 chemical reaction reagent Substances 0.000 claims description 50
239000007800 oxidant agent Substances 0.000 claims description 27
230000001590 oxidative effect Effects 0.000 claims description 24
108010061951 Methemoglobin Proteins 0.000 claims description 18
238000012360 testing method Methods 0.000 claims description 16
150000001450 anions Chemical class 0.000 claims description 14
239000000203 mixture Substances 0.000 claims description 14
YAGKRVSRTSUGEY-UHFFFAOYSA-N ferricyanide Chemical group [Fe+3].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-] YAGKRVSRTSUGEY-UHFFFAOYSA-N 0.000 claims description 6
229910001486 lithium perchlorate Inorganic materials 0.000 claims description 6
MHCFAGZWMAWTNR-UHFFFAOYSA-M lithium perchlorate Chemical compound [Li+].[O-]Cl(=O)(=O)=O MHCFAGZWMAWTNR-UHFFFAOYSA-M 0.000 claims description 5
238000004458 analytical method Methods 0.000 claims description 3
239000007864 aqueous solution Substances 0.000 claims 2
-1 lithium thiocyanate Chemical class 0.000 abstract description 17
238000010790 dilution Methods 0.000 abstract description 11
239000012895 dilution Substances 0.000 abstract description 11
239000000523 sample Substances 0.000 description 39
239000000243 solution Substances 0.000 description 26
DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 18
ZMZDMBWJUHKJPS-UHFFFAOYSA-M Thiocyanate anion Chemical compound [S-]C#N ZMZDMBWJUHKJPS-UHFFFAOYSA-M 0.000 description 18
238000002835 absorbance Methods 0.000 description 18
238000003556 assay Methods 0.000 description 18
ZMZDMBWJUHKJPS-UHFFFAOYSA-N hydrogen thiocyanate Natural products SC#N ZMZDMBWJUHKJPS-UHFFFAOYSA-N 0.000 description 18
239000004816 latex Substances 0.000 description 15
229920000126 latex Polymers 0.000 description 15
239000003398 denaturant Substances 0.000 description 12
WHXSMMKQMYFTQS-UHFFFAOYSA-N Lithium Chemical compound [Li] WHXSMMKQMYFTQS-UHFFFAOYSA-N 0.000 description 11
238000006243 chemical reaction Methods 0.000 description 11
229910052744 lithium Inorganic materials 0.000 description 10
229920000642 polymer Polymers 0.000 description 10
239000004471 Glycine Substances 0.000 description 9
DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 9
229910052708 sodium Inorganic materials 0.000 description 9
239000011734 sodium Substances 0.000 description 9
150000001768 cations Chemical class 0.000 description 8
ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 7
239000000872 buffer Substances 0.000 description 7
229910052700 potassium Inorganic materials 0.000 description 7
239000011591 potassium Substances 0.000 description 7
QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 description 6
238000005259 measurement Methods 0.000 description 6
239000002245 particle Substances 0.000 description 6
150000003839 salts Chemical class 0.000 description 6
150000003567 thiocyanates Chemical class 0.000 description 6
ZMXDDKWLCZADIW-UHFFFAOYSA-N N,N-Dimethylformamide Chemical compound CN(C)C=O ZMXDDKWLCZADIW-UHFFFAOYSA-N 0.000 description 5
239000012491 analyte Substances 0.000 description 5
239000008188 pellet Substances 0.000 description 5
108090000765 processed proteins & peptides Proteins 0.000 description 5
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 5
WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 4
ZRALSGWEFCBTJO-UHFFFAOYSA-N Guanidine Chemical compound NC(N)=N ZRALSGWEFCBTJO-UHFFFAOYSA-N 0.000 description 4
238000001514 detection method Methods 0.000 description 4
230000000694 effects Effects 0.000 description 4
239000008103 glucose Substances 0.000 description 4
238000002156 mixing Methods 0.000 description 4
VLTRZXGMWDSKGL-UHFFFAOYSA-M perchlorate Inorganic materials [O-]Cl(=O)(=O)=O VLTRZXGMWDSKGL-UHFFFAOYSA-M 0.000 description 4
VLTRZXGMWDSKGL-UHFFFAOYSA-N perchloric acid Chemical compound OCl(=O)(=O)=O VLTRZXGMWDSKGL-UHFFFAOYSA-N 0.000 description 4
230000009257 reactivity Effects 0.000 description 4
238000003756 stirring Methods 0.000 description 4
239000006228 supernatant Substances 0.000 description 4
239000000725 suspension Substances 0.000 description 4
QTBSBXVTEAMEQO-UHFFFAOYSA-M Acetate Chemical compound CC([O-])=O QTBSBXVTEAMEQO-UHFFFAOYSA-M 0.000 description 3
VEXZGXHMUGYJMC-UHFFFAOYSA-M Chloride anion Chemical compound [Cl-] VEXZGXHMUGYJMC-UHFFFAOYSA-M 0.000 description 3
102000017011 Glycated Hemoglobin A Human genes 0.000 description 3
229920000805 Polyaspartic acid Polymers 0.000 description 3
206010012601 diabetes mellitus Diseases 0.000 description 3
238000007865 diluting Methods 0.000 description 3
235000018102 proteins Nutrition 0.000 description 3
102000004169 proteins and genes Human genes 0.000 description 3
108090000623 proteins and genes Proteins 0.000 description 3
XKRFYHLGVUSROY-UHFFFAOYSA-N Argon Chemical compound [Ar] XKRFYHLGVUSROY-UHFFFAOYSA-N 0.000 description 2
XTEGARKTQYYJKE-UHFFFAOYSA-M Chlorate Chemical compound [O-]Cl(=O)=O XTEGARKTQYYJKE-UHFFFAOYSA-M 0.000 description 2
RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 2
108010014663 Glycated Hemoglobin A Proteins 0.000 description 2
CHJJGSNFBQVOTG-UHFFFAOYSA-N N-methyl-guanidine Natural products CNC(N)=N CHJJGSNFBQVOTG-UHFFFAOYSA-N 0.000 description 2
239000004793 Polystyrene Substances 0.000 description 2
FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
WYURNTSHIVDZCO-UHFFFAOYSA-N Tetrahydrofuran Chemical compound C1CCOC1 WYURNTSHIVDZCO-UHFFFAOYSA-N 0.000 description 2
125000003277 amino group Chemical group 0.000 description 2
150000003863 ammonium salts Chemical class 0.000 description 2
238000013459 approach Methods 0.000 description 2
239000011248 coating agent Substances 0.000 description 2
238000000576 coating method Methods 0.000 description 2
SWSQBOPZIKWTGO-UHFFFAOYSA-N dimethylaminoamidine Natural products CN(C)C(N)=N SWSQBOPZIKWTGO-UHFFFAOYSA-N 0.000 description 2
XLYOFNOQVPJJNP-UHFFFAOYSA-M hydroxide Chemical compound [OH-] XLYOFNOQVPJJNP-UHFFFAOYSA-M 0.000 description 2
WQYVRQLZKVEZGA-UHFFFAOYSA-N hypochlorite Chemical compound Cl[O-] WQYVRQLZKVEZGA-UHFFFAOYSA-N 0.000 description 2
230000036046 immunoreaction Effects 0.000 description 2
238000001727 in vivo Methods 0.000 description 2
ICIWUVCWSCSTAQ-UHFFFAOYSA-M iodate Chemical compound [O-]I(=O)=O ICIWUVCWSCSTAQ-UHFFFAOYSA-M 0.000 description 2
238000012986 modification Methods 0.000 description 2
230000004048 modification Effects 0.000 description 2
230000003647 oxidation Effects 0.000 description 2
238000007254 oxidation reaction Methods 0.000 description 2
KHIWWQKSHDUIBK-UHFFFAOYSA-N periodic acid Chemical compound OI(=O)(=O)=O KHIWWQKSHDUIBK-UHFFFAOYSA-N 0.000 description 2
229920002223 polystyrene Polymers 0.000 description 2
239000000047 product Substances 0.000 description 2
159000000000 sodium salts Chemical group 0.000 description 2
239000011550 stock solution Substances 0.000 description 2
239000000126 substance Substances 0.000 description 2
108700004154 thiocyanomethemoglobin Proteins 0.000 description 2
239000012224 working solution Substances 0.000 description 2
NHJVRSWLHSJWIN-UHFFFAOYSA-N 2,4,6-trinitrobenzenesulfonic acid Chemical compound OS(=O)(=O)C1=C([N+]([O-])=O)C=C([N+]([O-])=O)C=C1[N+]([O-])=O NHJVRSWLHSJWIN-UHFFFAOYSA-N 0.000 description 1
HZAXFHJVJLSVMW-UHFFFAOYSA-N 2-Aminoethan-1-ol Chemical compound NCCO HZAXFHJVJLSVMW-UHFFFAOYSA-N 0.000 description 1
RVBUGGBMJDPOST-UHFFFAOYSA-N 2-thiobarbituric acid Chemical compound O=C1CC(=O)NC(=S)N1 RVBUGGBMJDPOST-UHFFFAOYSA-N 0.000 description 1
ZDIZZJCWLCMMCS-UHFFFAOYSA-N 3-hydroxyfuran-2-carbaldehyde Chemical compound Oc1ccoc1C=O ZDIZZJCWLCMMCS-UHFFFAOYSA-N 0.000 description 1
208000007848 Alcoholism Diseases 0.000 description 1
206010003445 Ascites Diseases 0.000 description 1
108091003079 Bovine Serum Albumin Proteins 0.000 description 1
QDHHCQZDFGDHMP-UHFFFAOYSA-N Chloramine Chemical compound ClN QDHHCQZDFGDHMP-UHFFFAOYSA-N 0.000 description 1
KRKNYBCHXYNGOX-UHFFFAOYSA-K Citrate Chemical compound [O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O KRKNYBCHXYNGOX-UHFFFAOYSA-K 0.000 description 1
XFXPMWWXUTWYJX-UHFFFAOYSA-N Cyanide Chemical compound N#[C-] XFXPMWWXUTWYJX-UHFFFAOYSA-N 0.000 description 1
FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 description 1
238000002965 ELISA Methods 0.000 description 1
102000004190 Enzymes Human genes 0.000 description 1
108090000790 Enzymes Proteins 0.000 description 1
CWYNVVGOOAEACU-UHFFFAOYSA-N Fe2+ Chemical compound [Fe+2] CWYNVVGOOAEACU-UHFFFAOYSA-N 0.000 description 1
101000945318 Homo sapiens Calponin-1 Proteins 0.000 description 1
101000652736 Homo sapiens Transgelin Proteins 0.000 description 1
AVXURJPOCDRRFD-UHFFFAOYSA-N Hydroxylamine Chemical compound ON AVXURJPOCDRRFD-UHFFFAOYSA-N 0.000 description 1
OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 description 1
229930195725 Mannitol Natural products 0.000 description 1
125000000729 N-terminal amino-acid group Chemical group 0.000 description 1
229910020939 NaC104 Inorganic materials 0.000 description 1
108091005804 Peptidases Proteins 0.000 description 1
239000004743 Polypropylene Substances 0.000 description 1
239000004365 Protease Substances 0.000 description 1
102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 1
102100031013 Transgelin Human genes 0.000 description 1
238000011481 absorbance measurement Methods 0.000 description 1
238000010521 absorption reaction Methods 0.000 description 1
159000000021 acetate salts Chemical class 0.000 description 1
230000009471 action Effects 0.000 description 1
238000001042 affinity chromatography Methods 0.000 description 1
230000004520 agglutination Effects 0.000 description 1
206010001584 alcohol abuse Diseases 0.000 description 1
208000025746 alcohol use disease Diseases 0.000 description 1
150000001413 amino acids Chemical group 0.000 description 1
SOIFLUNRINLCBN-UHFFFAOYSA-N ammonium thiocyanate Chemical compound [NH4+].[S-]C#N SOIFLUNRINLCBN-UHFFFAOYSA-N 0.000 description 1
238000012801 analytical assay Methods 0.000 description 1
230000003466 anti-cipated effect Effects 0.000 description 1
229910052786 argon Inorganic materials 0.000 description 1
238000000149 argon plasma sintering Methods 0.000 description 1
239000012131 assay buffer Substances 0.000 description 1
230000008033 biological extinction Effects 0.000 description 1
229940098773 bovine serum albumin Drugs 0.000 description 1
SXDBWCPKPHAZSM-UHFFFAOYSA-M bromate Inorganic materials [O-]Br(=O)=O SXDBWCPKPHAZSM-UHFFFAOYSA-M 0.000 description 1
SXDBWCPKPHAZSM-UHFFFAOYSA-N bromic acid Chemical compound OBr(=O)=O SXDBWCPKPHAZSM-UHFFFAOYSA-N 0.000 description 1
238000005277 cation exchange chromatography Methods 0.000 description 1
125000002091 cationic group Chemical group 0.000 description 1
230000008859 change Effects 0.000 description 1
239000003795 chemical substances by application Substances 0.000 description 1
ZCDOYSPFYFSLEW-UHFFFAOYSA-N chromate(2-) Chemical compound [O-][Cr]([O-])(=O)=O ZCDOYSPFYFSLEW-UHFFFAOYSA-N 0.000 description 1
238000004587 chromatography analysis Methods 0.000 description 1
239000007979 citrate buffer Substances 0.000 description 1
238000010668 complexation reaction Methods 0.000 description 1
239000000470 constituent Substances 0.000 description 1
238000010276 construction Methods 0.000 description 1
239000012043 crude product Substances 0.000 description 1
230000007423 decrease Effects 0.000 description 1
238000011033 desalting Methods 0.000 description 1
238000003745 diagnosis Methods 0.000 description 1
239000012470 diluted sample Substances 0.000 description 1
239000003085 diluting agent Substances 0.000 description 1
239000012153 distilled water Substances 0.000 description 1
238000001962 electrophoresis Methods 0.000 description 1
238000001704 evaporation Methods 0.000 description 1
230000008020 evaporation Effects 0.000 description 1
238000002474 experimental method Methods 0.000 description 1
229910001448 ferrous ion Inorganic materials 0.000 description 1
238000001914 filtration Methods 0.000 description 1
239000012530 fluid Substances 0.000 description 1
239000012634 fragment Substances 0.000 description 1
108091005995 glycated hemoglobin Proteins 0.000 description 1
150000003278 haem Chemical class 0.000 description 1
238000010438 heat treatment Methods 0.000 description 1
XMBWDFGMSWQBCA-UHFFFAOYSA-N hydrogen iodide Chemical compound I XMBWDFGMSWQBCA-UHFFFAOYSA-N 0.000 description 1
150000004679 hydroxides Chemical class 0.000 description 1
239000005457 ice water Substances 0.000 description 1
230000005764 inhibitory process Effects 0.000 description 1
238000009413 insulation Methods 0.000 description 1
230000003993 interaction Effects 0.000 description 1
230000002452 interceptive effect Effects 0.000 description 1
238000002372 labelling Methods 0.000 description 1
239000007788 liquid Substances 0.000 description 1
230000007774 longterm Effects 0.000 description 1
238000007726 management method Methods 0.000 description 1
239000000594 mannitol Substances 0.000 description 1
235000010355 mannitol Nutrition 0.000 description 1
230000007935 neutral effect Effects 0.000 description 1
150000002978 peroxides Chemical class 0.000 description 1
229920001155 polypropylene Polymers 0.000 description 1
125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 1
230000008569 process Effects 0.000 description 1
230000002035 prolonged effect Effects 0.000 description 1
230000004044 response Effects 0.000 description 1
239000012266 salt solution Substances 0.000 description 1
238000000926 separation method Methods 0.000 description 1
239000011780 sodium chloride Substances 0.000 description 1
VGTPCRGMBIAPIM-UHFFFAOYSA-M sodium thiocyanate Chemical compound [Na+].[S-]C#N VGTPCRGMBIAPIM-UHFFFAOYSA-M 0.000 description 1
238000000527 sonication Methods 0.000 description 1
239000003381 stabilizer Substances 0.000 description 1
JJAHTWIKCUJRDK-UHFFFAOYSA-N succinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate Chemical compound C1CC(CN2C(C=CC2=O)=O)CCC1C(=O)ON1C(=O)CCC1=O JJAHTWIKCUJRDK-UHFFFAOYSA-N 0.000 description 1
YLQBMQCUIZJEEH-UHFFFAOYSA-N tetrahydrofuran Natural products C=1C=COC=1 YLQBMQCUIZJEEH-UHFFFAOYSA-N 0.000 description 1
229910052716 thallium Inorganic materials 0.000 description 1
OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 description 1
125000002987 valine group Chemical group [H]N([H])C([H])(C(*)=O)C([H])(C([H])([H])[H])C([H])([H])[H] 0.000 description 1
238000003260 vortexing Methods 0.000 description 1
238000005406 washing Methods 0.000 description 1

Classifications

- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5002—Partitioning blood components
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/72—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood pigments, e.g. haemoglobin, bilirubin or other porphyrins; involving occult blood
- G01N33/721—Haemoglobin
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/72—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood pigments, e.g. haemoglobin, bilirubin or other porphyrins; involving occult blood
- G01N33/721—Haemoglobin
- G01N33/723—Glycosylated haemoglobin

Landscapes

Health & Medical Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Engineering & Computer Science (AREA)
Hematology (AREA)
Molecular Biology (AREA)
Biomedical Technology (AREA)
Chemical & Material Sciences (AREA)
Immunology (AREA)
Urology & Nephrology (AREA)
Food Science & Technology (AREA)
Biochemistry (AREA)
Cell Biology (AREA)
Biotechnology (AREA)
Medicinal Chemistry (AREA)
Physics & Mathematics (AREA)
Analytical Chemistry (AREA)
Microbiology (AREA)
General Health & Medical Sciences (AREA)
General Physics & Mathematics (AREA)
Pathology (AREA)
Investigating Or Analysing Biological Materials (AREA)
Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Saccharide Compounds (AREA)

IL9472490A 1989-07-13 1990-06-13 Decomposition of red blood cells and denaturation of hemoglobin by the use of lithium salts IL94724A (en)

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
US37958289A	1989-07-13	1989-07-13

Publications (2)

Publication Number	Publication Date
IL94724A0 IL94724A0 (en)	1991-04-15
IL94724A true IL94724A (en)	1994-02-27

Family

ID=23497825

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
IL9472490A IL94724A (en)	1989-07-13	1990-06-13	Decomposition of red blood cells and denaturation of hemoglobin by the use of lithium salts

Country Status (12)

Country	Link
EP (1)	EP0407860B1 (de)
JP (1)	JP2843919B2 (de)
AT (1)	ATE145991T1 (de)
AU (1)	AU625137B2 (de)
DE (1)	DE69029291T2 (de)
DK (1)	DK0407860T3 (de)
ES (1)	ES2095224T3 (de)
FI (1)	FI102698B (de)
GR (1)	GR3022522T3 (de)
HU (1)	HU206157B (de)
IE (1)	IE902549A1 (de)
IL (1)	IL94724A (de)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US5151369A (en) *	1989-07-13	1992-09-29	Miles Inc.	Lithium salts as red blood cell lysing and hemoglobin denaturing reagents
US5236664A (en) *	1991-04-08	1993-08-17	University Of South Alabama	Apparatus for monitoring blood loss
DE4206932A1 (de) *	1992-03-05	1993-09-09	Boehringer Mannheim Gmbh	Immunologische methode zur bestimmung eines haemoglobinderivates
US5610076A (en) *	1994-04-29	1997-03-11	Alteon Inc.	Method for detecting hemoglobin advanced glycosylation endproducts
US6174734B1 (en)	1997-10-24	2001-01-16	Abbott Laboratories	Measurement of glycated hemoglobin
CN1522369A (zh) *	2002-03-29	2004-08-18	��µ��ҵ��ʽ��	血液处理方法及其装置、血红蛋白类测定方法及其装置
JP4957547B2 (ja)	2005-04-14	2012-06-20	パナソニック株式会社	ヘモグロビン誘導体の測定方法、それに用いる試薬組成物、測定キット、分析デバイス、及び分析システム
EP3141901B1 (de)	2007-10-04	2018-12-12	PHC Holdings Corporation	Analysevorrichtung und analyseeinrichtung und verfahren zur verwendung davon
CN103353534A (zh)	2007-10-30	2013-10-16	松下电器产业株式会社	血红蛋白及血红蛋白衍生物的测定方法、测定试剂盒
WO2010067612A1 (ja)	2008-12-11	2010-06-17	積水メディカル株式会社	糖化ヘモグロビン含有試料の前処理法
WO2020163211A1 (en) *	2019-02-06	2020-08-13	Siemens Healthcare Diagnostics Inc.	Methods of blood sample suspension

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
CA1339952C (en) *	1984-10-29	1998-07-14	William J. Knowles	Immunoassays for denatured protein analytes, particularly hb alc, and monoclonal antibodies thereto
US4970171A (en) *	1987-11-09	1990-11-13	Miles Inc.	Denaturant reagents for convenient determination of hemoglobin derivatives in blood

1990
- 1990-06-13 IL IL9472490A patent/IL94724A/en unknown
- 1990-07-03 ES ES90112657T patent/ES2095224T3/es not_active Expired - Lifetime
- 1990-07-03 EP EP90112657A patent/EP0407860B1/de not_active Expired - Lifetime
- 1990-07-03 DK DK90112657.3T patent/DK0407860T3/da active
- 1990-07-03 DE DE69029291T patent/DE69029291T2/de not_active Expired - Lifetime
- 1990-07-03 AT AT90112657T patent/ATE145991T1/de not_active IP Right Cessation
- 1990-07-11 FI FI903510A patent/FI102698B/fi not_active IP Right Cessation
- 1990-07-11 AU AU58909/90A patent/AU625137B2/en not_active Ceased
- 1990-07-12 JP JP2182899A patent/JP2843919B2/ja not_active Expired - Lifetime
- 1990-07-12 IE IE254990A patent/IE902549A1/en not_active IP Right Cessation
- 1990-07-12 HU HU904183A patent/HU206157B/hu not_active IP Right Cessation
1997
- 1997-02-12 GR GR960403601T patent/GR3022522T3/el unknown

Also Published As

Publication number	Publication date
DE69029291D1 (de)	1997-01-16
DE69029291T2 (de)	1997-04-03
FI102698B1 (fi)	1999-01-29
DK0407860T3 (da)	1997-05-12
ATE145991T1 (de)	1996-12-15
JP2843919B2 (ja)	1999-01-06
ES2095224T3 (es)	1997-02-16
FI903510A0 (fi)	1990-07-11
IL94724A0 (en)	1991-04-15
EP0407860B1 (de)	1996-12-04
EP0407860A2 (de)	1991-01-16
HU904183D0 (en)	1990-12-28
HU206157B (en)	1992-08-28
JPH0351759A (ja)	1991-03-06
EP0407860A3 (en)	1992-03-04
AU5890990A (en)	1991-01-17
HUT57902A (en)	1991-12-30
FI102698B (fi)	1999-01-29
GR3022522T3 (en)	1997-05-31
AU625137B2 (en)	1992-07-02
IE902549A1 (en)	1991-02-27

Legal Events

Date	Code	Title
1996-10-16	KB	Patent renewed
2004-07-25	KB	Patent renewed
2008-11-03	KB	Patent renewed

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Powell et al.	1972	A new automated procedure for the colorimetric determination of glucose
JP3181390B2 (ja)	2001-07-03	タンパク質の糖化割合の測定方法
WO1996034290A1 (en)	1996-10-31	Haemoglobin standards
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