IT1221765B - Sequenza nucleotidica capace di indurre alti livelli di traduzione di un gene eterologo in bacillus subtilis ed escherichia coli - Google Patents

Sequenza nucleotidica capace di indurre alti livelli di traduzione di un gene eterologo in bacillus subtilis ed escherichia coli

Info

Publication number
IT1221765B
IT1221765B IT20344/86A IT2034486A IT1221765B IT 1221765 B IT1221765 B IT 1221765B IT 20344/86 A IT20344/86 A IT 20344/86A IT 2034486 A IT2034486 A IT 2034486A IT 1221765 B IT1221765 B IT 1221765B
Authority
IT
Italy
Prior art keywords
escherichia coli
bacillus subtilis
translation
high levels
inducing high
Prior art date
Application number
IT20344/86A
Other languages
English (en)
Other versions
IT8620344A0 (it
Inventor
Grandi Guido
Original Assignee
Eniricerche Spa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Eniricerche Spa filed Critical Eniricerche Spa
Priority to IT20344/86A priority Critical patent/IT1221765B/it
Publication of IT8620344A0 publication Critical patent/IT8620344A0/it
Priority to AT87830141T priority patent/ATE73496T1/de
Priority to EP87830141A priority patent/EP0245217B1/en
Priority to DE8787830141T priority patent/DE3777238D1/de
Priority to ES198787830141T priority patent/ES2032862T3/es
Priority to US07/040,612 priority patent/US4987069A/en
Priority to JP62109915A priority patent/JP2588402B2/ja
Application granted granted Critical
Publication of IT1221765B publication Critical patent/IT1221765B/it
Priority to GR920400908T priority patent/GR3004560T3/el

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C12N9/86—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in cyclic amides, e.g. penicillinase (3.5.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Saccharide Compounds (AREA)
IT20344/86A 1986-05-07 1986-05-07 Sequenza nucleotidica capace di indurre alti livelli di traduzione di un gene eterologo in bacillus subtilis ed escherichia coli IT1221765B (it)

Priority Applications (8)

Application Number Priority Date Filing Date Title
IT20344/86A IT1221765B (it) 1986-05-07 1986-05-07 Sequenza nucleotidica capace di indurre alti livelli di traduzione di un gene eterologo in bacillus subtilis ed escherichia coli
AT87830141T ATE73496T1 (de) 1986-05-07 1987-04-13 Nukleotidsequenz, die in der lage ist, hohe translationsniveaus eines heterologen gens in bacillus subtilis und escherichia coli zu induzieren.
EP87830141A EP0245217B1 (en) 1986-05-07 1987-04-13 A nucleotide sequence capable of inducing high levels of translation of a heterologous gene in bacillus subtilis and escherichia coli
DE8787830141T DE3777238D1 (de) 1986-05-07 1987-04-13 Nukleotidsequenz, die in der lage ist, hohe translationsniveaus eines heterologen gens in bacillus subtilis und escherichia coli zu induzieren.
ES198787830141T ES2032862T3 (es) 1986-05-07 1987-04-13 Una secuencia nucleotida capaz de inducir altos niveles de translacion de un gen heterologo en bacillus subtilis y escherichia coli.
US07/040,612 US4987069A (en) 1986-05-07 1987-04-21 Nucleotide sequence capable of inducing high levels of translation of a heterologous gene in Bacillus subtilis and Escherichia coli
JP62109915A JP2588402B2 (ja) 1986-05-07 1987-05-07 ヌクレオチド配列
GR920400908T GR3004560T3 (it) 1986-05-07 1992-05-12

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
IT20344/86A IT1221765B (it) 1986-05-07 1986-05-07 Sequenza nucleotidica capace di indurre alti livelli di traduzione di un gene eterologo in bacillus subtilis ed escherichia coli

Publications (2)

Publication Number Publication Date
IT8620344A0 IT8620344A0 (it) 1986-05-07
IT1221765B true IT1221765B (it) 1990-07-12

Family

ID=11165894

Family Applications (1)

Application Number Title Priority Date Filing Date
IT20344/86A IT1221765B (it) 1986-05-07 1986-05-07 Sequenza nucleotidica capace di indurre alti livelli di traduzione di un gene eterologo in bacillus subtilis ed escherichia coli

Country Status (8)

Country Link
US (1) US4987069A (it)
EP (1) EP0245217B1 (it)
JP (1) JP2588402B2 (it)
AT (1) ATE73496T1 (it)
DE (1) DE3777238D1 (it)
ES (1) ES2032862T3 (it)
GR (1) GR3004560T3 (it)
IT (1) IT1221765B (it)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5279947A (en) * 1988-08-24 1994-01-18 Sanofi DNA sequence participating in the regulation of the expression of a DNA sequence coding for a precursor of a polypeptide, expression vectors and process for the periplasmic production of the polypeptide
FR2636644B1 (fr) * 1988-08-24 1990-12-28 Sanofi Sa Sequence d'adn participant a la regulation de l'expression d'une sequence d'adn codant pour un precurseur d'un polypeptide, vecteurs d'expression et procede de production periplasmique du polypeptide

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4529695A (en) * 1981-06-18 1985-07-16 Wisconsin Alumni Research Foundation Recombinant cloning vector, host for the vector, and method for using same
IT1156317B (it) * 1982-09-08 1987-02-04 Anic Spa Vettori plasmidici ad espressione in bacillus subtilis e procedimento per la loro preparazione
JPS60188077A (ja) * 1984-03-09 1985-09-25 Teruhiko Beppu 仔牛プロキモシンｃＤΝＡの全配列を有する新規な発現プラスミド

Also Published As

Publication number Publication date
GR3004560T3 (it) 1993-04-28
JP2588402B2 (ja) 1997-03-05
DE3777238D1 (de) 1992-04-16
EP0245217A2 (en) 1987-11-11
US4987069A (en) 1991-01-22
ES2032862T3 (es) 1993-03-01
EP0245217B1 (en) 1992-03-11
JPS63219383A (ja) 1988-09-13
EP0245217A3 (en) 1988-11-09
ATE73496T1 (de) 1992-03-15
IT8620344A0 (it) 1986-05-07

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