IT1248565B - Anticorpi policlonali anti-irudina e loro utilizzo per la identificazione, l`immunopurificazione e la determinazione quantitativa dell`uridina. - Google Patents

Anticorpi policlonali anti-irudina e loro utilizzo per la identificazione, l`immunopurificazione e la determinazione quantitativa dell`uridina.

Info

Publication number
IT1248565B
IT1248565B ITMI911767A ITMI911767A IT1248565B IT 1248565 B IT1248565 B IT 1248565B IT MI911767 A ITMI911767 A IT MI911767A IT MI911767 A ITMI911767 A IT MI911767A IT 1248565 B IT1248565 B IT 1248565B
Authority
IT
Italy
Prior art keywords
identification
quantitative determination
hiruds
immunogenic
aforementioned
Prior art date
Application number
ITMI911767A
Other languages
English (en)
Inventor
Antonio Molinari
Marco Gerna
Carla Giorgietti
Jacqueline Lansen
Romeo Roncucci
Original Assignee
Erba Carlo Spa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Erba Carlo Spa filed Critical Erba Carlo Spa
Publication of ITMI911767A0 publication Critical patent/ITMI911767A0/it
Priority to ITMI911767A priority Critical patent/IT1248565B/it
Priority to MX9203242A priority patent/MX9203242A/es
Priority to DE69221882T priority patent/DE69221882T2/de
Priority to ZA924773A priority patent/ZA924773B/xx
Priority to ES92914181T priority patent/ES2107545T3/es
Priority to EP19920110856 priority patent/EP0520502A3/en
Priority to AU21937/92A priority patent/AU2193792A/en
Priority to EP92914181A priority patent/EP0600901B1/en
Priority to PCT/EP1992/001448 priority patent/WO1993000363A1/en
Priority to JP5501337A priority patent/JPH06509070A/ja
Priority to NZ243355A priority patent/NZ243355A/xx
Priority to IE208192A priority patent/IE922081A1/en
Publication of ITMI911767A1 publication Critical patent/ITMI911767A1/it
Application granted granted Critical
Publication of IT1248565B publication Critical patent/IT1248565B/it
Priority to US08/603,182 priority patent/US6719975B1/en
Priority to US10/767,200 priority patent/US20050095685A1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/81—Protease inhibitors
    • C07K14/815—Protease inhibitors from leeches, e.g. hirudin, eglin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/38—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against protease inhibitors of peptide structure

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

La presente invenzione riguarda un metodo semplice e riproducibile per ottenere anticorpi policlonali specifici per irudina ed isoforme e proteine irudino-simili ad attività antitrombinica, derivate da diverse specie di sanguisuga.Oggetto dell'invenzione è un metodo per rendere immunogeniche le irudine di cui sopra mediante polimerizzazione, e le irudine immunogeniche così ottenute.L'invenzione prevede quindi l'immunizzazione di un animale sperimentale con le suddette irudine immunogeniche, la purificazione delle IgG dal siero, e il loro utilizzo nello sviluppo di metodiche immunologiche per la identificazione, la purificazione, e la determinazione quantitativa delle suddette irudine, ad esempio nei liquidi biologici.
ITMI911767A 1991-06-27 1991-06-27 Anticorpi policlonali anti-irudina e loro utilizzo per la identificazione, l`immunopurificazione e la determinazione quantitativa dell`uridina. IT1248565B (it)

Priority Applications (14)

Application Number Priority Date Filing Date Title
ITMI911767A IT1248565B (it) 1991-06-27 1991-06-27 Anticorpi policlonali anti-irudina e loro utilizzo per la identificazione, l`immunopurificazione e la determinazione quantitativa dell`uridina.
MX9203242A MX9203242A (es) 1991-06-27 1992-06-24 Anticuerpos policlonales anti-hirudin y metodo para su produccion.
AU21937/92A AU2193792A (en) 1991-06-27 1992-06-26 Anti-hirudin polyclonal antibodies and their use for the identification, immunopurification and quantitative determination of hirudin
JP5501337A JPH06509070A (ja) 1991-06-27 1992-06-26 抗ヒルジンポリクローナル抗体ならびにヒルジンの同定、免疫精製および定量的測定のためのその用途
ES92914181T ES2107545T3 (es) 1991-06-27 1992-06-26 Anticuerpos policlonales dirigidos contra la hirudina y su utilizacion para la identificacion, la purificacion inmunologica y la determinacion cuantitativa de la hirudina.
EP19920110856 EP0520502A3 (en) 1991-06-27 1992-06-26 Anti-hirudin polyclonal antibodies and their use for the identification, immunopurification and quantitative determination of hirudin
DE69221882T DE69221882T2 (de) 1991-06-27 1992-06-26 Polyklonale anti-hirudin antikörper und ihre verwendung in der identifizierung und quantitativen bestimmung von hirudin
EP92914181A EP0600901B1 (en) 1991-06-27 1992-06-26 Anti-hirudin polyclonal antibodies and their use for the identification, immunopurification and quantitative determination of hirudin
PCT/EP1992/001448 WO1993000363A1 (en) 1991-06-27 1992-06-26 Anti-hirudin polyclonal antibodies and their use for the identification, immunopurification and quantitative determination of hirudin
ZA924773A ZA924773B (en) 1991-06-27 1992-06-26 Anti-hirudin polyclonal antibodies and their use for the identification,immunopurification and quantitative determination of hirudin
NZ243355A NZ243355A (en) 1991-06-27 1992-06-29 Hirudin or hirudin-like protein polymer immunogen and its use in raising antibodies against hirudin
IE208192A IE922081A1 (en) 1991-06-27 1992-07-01 Anti-hirudin polyclonal antibodies and their use for the¹identification, immunopurification and quantitative¹determination of hirudin
US08/603,182 US6719975B1 (en) 1991-06-27 1996-02-20 Anti-hirudin polyclonal antibodies and their use for the identification, immunopurification and quantitative determination of hirudin
US10/767,200 US20050095685A1 (en) 1991-06-27 2004-01-30 Anti-hirudin polyclonal antibodies and their use for the identification, immunopurification and quantitative determination of hirudin

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ITMI911767A IT1248565B (it) 1991-06-27 1991-06-27 Anticorpi policlonali anti-irudina e loro utilizzo per la identificazione, l`immunopurificazione e la determinazione quantitativa dell`uridina.

Publications (3)

Publication Number Publication Date
ITMI911767A0 ITMI911767A0 (it) 1991-06-27
ITMI911767A1 ITMI911767A1 (it) 1992-12-27
IT1248565B true IT1248565B (it) 1995-01-19

Family

ID=11360217

Family Applications (1)

Application Number Title Priority Date Filing Date
ITMI911767A IT1248565B (it) 1991-06-27 1991-06-27 Anticorpi policlonali anti-irudina e loro utilizzo per la identificazione, l`immunopurificazione e la determinazione quantitativa dell`uridina.

Country Status (11)

Country Link
EP (2) EP0600901B1 (it)
JP (1) JPH06509070A (it)
AU (1) AU2193792A (it)
DE (1) DE69221882T2 (it)
ES (1) ES2107545T3 (it)
IE (1) IE922081A1 (it)
IT (1) IT1248565B (it)
MX (1) MX9203242A (it)
NZ (1) NZ243355A (it)
WO (1) WO1993000363A1 (it)
ZA (1) ZA924773B (it)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IT1266561B1 (it) * 1993-07-22 1997-01-09 Mini Ricerca Scient Tecnolog Analoghi di un polipeptide anti-trombinico e procedimento per la loro preparazione
AU685835B2 (en) * 1993-08-04 1998-01-29 Novartis Ag High molecular weight desulphatohirudin
US5710413A (en) * 1995-03-29 1998-01-20 Minnesota Mining And Manufacturing Company H-field electromagnetic heating system for fusion bonding
CN115572329B (zh) * 2021-06-21 2024-02-06 王大勇 一组活性增强代谢较慢的菲牛蛭基因重组水蛭素及其制备方法

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU554859B2 (en) * 1981-09-04 1986-09-04 Regents Of The University Of California, The Polymerized albumin vaccine
EP0380443B2 (en) * 1989-01-25 1999-06-30 Novartis AG Monoclonal antibodies specific for thrombin hirudin

Also Published As

Publication number Publication date
WO1993000363A1 (en) 1993-01-07
ITMI911767A1 (it) 1992-12-27
EP0520502A3 (en) 1993-06-23
DE69221882D1 (de) 1997-10-02
MX9203242A (es) 1994-04-29
ES2107545T3 (es) 1997-12-01
NZ243355A (en) 1993-09-27
EP0600901B1 (en) 1997-08-27
IE922081A1 (en) 1992-12-30
EP0600901A1 (en) 1994-06-15
JPH06509070A (ja) 1994-10-13
ITMI911767A0 (it) 1991-06-27
AU2193792A (en) 1993-01-25
EP0520502A2 (en) 1992-12-30
DE69221882T2 (de) 1998-01-02
ZA924773B (en) 1993-03-31

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0001 Granted
TA Fee payment date (situation as of event date), data collected since 19931001

Effective date: 19970527