IT1248565B - Anticorpi policlonali anti-irudina e loro utilizzo per la identificazione, l`immunopurificazione e la determinazione quantitativa dell`uridina. - Google Patents

Anticorpi policlonali anti-irudina e loro utilizzo per la identificazione, l`immunopurificazione e la determinazione quantitativa dell`uridina.

Info

Publication number
IT1248565B
IT1248565B ITMI911767A ITMI911767A IT1248565B IT 1248565 B IT1248565 B IT 1248565B IT MI911767 A ITMI911767 A IT MI911767A IT MI911767 A ITMI911767 A IT MI911767A IT 1248565 B IT1248565 B IT 1248565B
Authority
IT
Italy
Prior art keywords
identification
quantitative determination
hiruds
immunogenic
aforementioned
Prior art date
Application number
ITMI911767A
Other languages
English (en)
Inventor
Antonio Molinari
Marco Gerna
Carla Giorgietti
Jacqueline Lansen
Romeo Roncucci
Original Assignee
Erba Carlo Spa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Erba Carlo Spa filed Critical Erba Carlo Spa
Publication of ITMI911767A0 publication Critical patent/ITMI911767A0/it
Priority to ITMI911767A priority Critical patent/IT1248565B/it
Priority to MX9203242A priority patent/MX9203242A/es
Priority to DE69221882T priority patent/DE69221882T2/de
Priority to PCT/EP1992/001448 priority patent/WO1993000363A1/en
Priority to JP5501337A priority patent/JPH06509070A/ja
Priority to EP92914181A priority patent/EP0600901B1/en
Priority to EP19920110856 priority patent/EP0520502A3/en
Priority to ES92914181T priority patent/ES2107545T3/es
Priority to ZA924773A priority patent/ZA924773B/xx
Priority to AU21937/92A priority patent/AU2193792A/en
Priority to NZ243355A priority patent/NZ243355A/xx
Priority to IE208192A priority patent/IE922081A1/en
Publication of ITMI911767A1 publication Critical patent/ITMI911767A1/it
Application granted granted Critical
Publication of IT1248565B publication Critical patent/IT1248565B/it
Priority to US08/603,182 priority patent/US6719975B1/en
Priority to US10/767,200 priority patent/US20050095685A1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/81—Protease inhibitors
    • C07K14/815—Protease inhibitors from leeches, e.g. hirudin, eglin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/38—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against protease inhibitors of peptide structure

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

La presente invenzione riguarda un metodo semplice e riproducibile per ottenere anticorpi policlonali specifici per irudina ed isoforme e proteine irudino-simili ad attività antitrombinica, derivate da diverse specie di sanguisuga.Oggetto dell'invenzione è un metodo per rendere immunogeniche le irudine di cui sopra mediante polimerizzazione, e le irudine immunogeniche così ottenute.L'invenzione prevede quindi l'immunizzazione di un animale sperimentale con le suddette irudine immunogeniche, la purificazione delle IgG dal siero, e il loro utilizzo nello sviluppo di metodiche immunologiche per la identificazione, la purificazione, e la determinazione quantitativa delle suddette irudine, ad esempio nei liquidi biologici.
ITMI911767A 1991-06-27 1991-06-27 Anticorpi policlonali anti-irudina e loro utilizzo per la identificazione, l`immunopurificazione e la determinazione quantitativa dell`uridina. IT1248565B (it)

Priority Applications (14)

Application Number Priority Date Filing Date Title
ITMI911767A IT1248565B (it) 1991-06-27 1991-06-27 Anticorpi policlonali anti-irudina e loro utilizzo per la identificazione, l`immunopurificazione e la determinazione quantitativa dell`uridina.
MX9203242A MX9203242A (es) 1991-06-27 1992-06-24 Anticuerpos policlonales anti-hirudin y metodo para su produccion.
EP19920110856 EP0520502A3 (en) 1991-06-27 1992-06-26 Anti-hirudin polyclonal antibodies and their use for the identification, immunopurification and quantitative determination of hirudin
AU21937/92A AU2193792A (en) 1991-06-27 1992-06-26 Anti-hirudin polyclonal antibodies and their use for the identification, immunopurification and quantitative determination of hirudin
JP5501337A JPH06509070A (ja) 1991-06-27 1992-06-26 抗ヒルジンポリクローナル抗体ならびにヒルジンの同定、免疫精製および定量的測定のためのその用途
EP92914181A EP0600901B1 (en) 1991-06-27 1992-06-26 Anti-hirudin polyclonal antibodies and their use for the identification, immunopurification and quantitative determination of hirudin
DE69221882T DE69221882T2 (de) 1991-06-27 1992-06-26 Polyklonale anti-hirudin antikörper und ihre verwendung in der identifizierung und quantitativen bestimmung von hirudin
ES92914181T ES2107545T3 (es) 1991-06-27 1992-06-26 Anticuerpos policlonales dirigidos contra la hirudina y su utilizacion para la identificacion, la purificacion inmunologica y la determinacion cuantitativa de la hirudina.
ZA924773A ZA924773B (en) 1991-06-27 1992-06-26 Anti-hirudin polyclonal antibodies and their use for the identification,immunopurification and quantitative determination of hirudin
PCT/EP1992/001448 WO1993000363A1 (en) 1991-06-27 1992-06-26 Anti-hirudin polyclonal antibodies and their use for the identification, immunopurification and quantitative determination of hirudin
NZ243355A NZ243355A (en) 1991-06-27 1992-06-29 Hirudin or hirudin-like protein polymer immunogen and its use in raising antibodies against hirudin
IE208192A IE922081A1 (en) 1991-06-27 1992-07-01 Anti-hirudin polyclonal antibodies and their use for the¹identification, immunopurification and quantitative¹determination of hirudin
US08/603,182 US6719975B1 (en) 1991-06-27 1996-02-20 Anti-hirudin polyclonal antibodies and their use for the identification, immunopurification and quantitative determination of hirudin
US10/767,200 US20050095685A1 (en) 1991-06-27 2004-01-30 Anti-hirudin polyclonal antibodies and their use for the identification, immunopurification and quantitative determination of hirudin

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ITMI911767A IT1248565B (it) 1991-06-27 1991-06-27 Anticorpi policlonali anti-irudina e loro utilizzo per la identificazione, l`immunopurificazione e la determinazione quantitativa dell`uridina.

Publications (3)

Publication Number Publication Date
ITMI911767A0 ITMI911767A0 (it) 1991-06-27
ITMI911767A1 ITMI911767A1 (it) 1992-12-27
IT1248565B true IT1248565B (it) 1995-01-19

Family

ID=11360217

Family Applications (1)

Application Number Title Priority Date Filing Date
ITMI911767A IT1248565B (it) 1991-06-27 1991-06-27 Anticorpi policlonali anti-irudina e loro utilizzo per la identificazione, l`immunopurificazione e la determinazione quantitativa dell`uridina.

Country Status (11)

Country Link
EP (2) EP0520502A3 (it)
JP (1) JPH06509070A (it)
AU (1) AU2193792A (it)
DE (1) DE69221882T2 (it)
ES (1) ES2107545T3 (it)
IE (1) IE922081A1 (it)
IT (1) IT1248565B (it)
MX (1) MX9203242A (it)
NZ (1) NZ243355A (it)
WO (1) WO1993000363A1 (it)
ZA (1) ZA924773B (it)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IT1266561B1 (it) * 1993-07-22 1997-01-09 Mini Ricerca Scient Tecnolog Analoghi di un polipeptide anti-trombinico e procedimento per la loro preparazione
US5661001A (en) * 1993-08-04 1997-08-26 Ciba-Geigy Corporation High molecular weight desulphatohirudin
US5710413A (en) * 1995-03-29 1998-01-20 Minnesota Mining And Manufacturing Company H-field electromagnetic heating system for fusion bonding
CN115572329B (zh) * 2021-06-21 2024-02-06 王大勇 一组活性增强代谢较慢的菲牛蛭基因重组水蛭素及其制备方法

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU554859B2 (en) * 1981-09-04 1986-09-04 Regents Of The University Of California, The Polymerized albumin vaccine
ES2069726T5 (es) * 1989-01-25 1999-09-16 Novartis Ag Anticuerpos monoclonales especificos para hirudina.

Also Published As

Publication number Publication date
ZA924773B (en) 1993-03-31
ES2107545T3 (es) 1997-12-01
ITMI911767A1 (it) 1992-12-27
NZ243355A (en) 1993-09-27
WO1993000363A1 (en) 1993-01-07
EP0600901A1 (en) 1994-06-15
MX9203242A (es) 1994-04-29
EP0520502A2 (en) 1992-12-30
DE69221882D1 (de) 1997-10-02
EP0520502A3 (en) 1993-06-23
AU2193792A (en) 1993-01-25
ITMI911767A0 (it) 1991-06-27
JPH06509070A (ja) 1994-10-13
EP0600901B1 (en) 1997-08-27
IE922081A1 (en) 1992-12-30
DE69221882T2 (de) 1998-01-02

Similar Documents

Publication Publication Date Title
Dingle et al. Cathepsin D. Characteristics of immunoinhibition and the confirmation of a role in cartilage breakdown
EP0129147B1 (en) High titer pseudomonas immune serum globulin and method of making same
DK158284A (da) Monoklonale antistoffer afledt af tvaerbundne fibrinderivater samt analysemetode til bestemmelse af disse derivater
US4548909A (en) Method of diagnosis
Barral-Netto et al. Enzyme-linked immunosorbent assay for the detection of Bothrops jararaca venom
Cupps et al. Activation of human peripheral blood B cells following immunization with hepatitis B surface antigen vaccine
Berger et al. Time course of hepatitis A antibody production after active, passive and active/passive immunisation: the results are highly dependent on the antibody test system used
JPS5530655A (en) Latex sensitive to human igg antibody for quantitizing human igg and its particle composite
Sirisinha et al. Immunological analysis of Opisthorchis and Clonorchis antigens
Delgado et al. Evaluation of an enzyme-linked immunosorbent assay for the detection of sheep infected and vaccinated with Brucella melitensis
FR2608767B1 (fr) Procede pour la detection et le dosage des anticorps anti-(sporozoaires de p. falciparum) dans le sang humain et trousses de diagnostic pour mettre en oeuvre ce procede
ZA872145B (en) Method for the detection of pregnancy disorders
DE3781421D1 (de) Verfahren zum nachweis von einer infektion oder entzuendung des harnbezueglichen tractus.
de Saint Martin et al. Titration of antibodies to tetanus toxoid by agglutination of purified tetanus toxoid sensitized latex particles
EP4628897A3 (en) Methods, compositions, and systems for detecting coronavirus neutralizing antibodies
Katz et al. Comparison of an enzyme-linked immunosorbent assay with radioimmunoassay for the measurement of pneumococcal capsular polysaccharide antibodies
Project The level of anti-sporozoite antibodies in a highly endemic malaria area and its relationship with exposure to mosquitoes
Williams et al. Soluble serum antigens of P. falciparum in Nigerians. I. Local incidence of malarial soluble serum antigens and antibodies.
Ozaki Neutralization kinetics of poliovirus by specific antiserum during the course of immunization of rabbits
ITMI911767A0 (it) Anticorpi policlonali anti-irudina e loro utilizzo per la identificazione, l'immunopurificazione e la determinazione quantitativa dell'irudina
Hoffmann et al. Intestinal alkaline phosphatase-like properties of horse kidney alkaline phosphatase
SU1396057A1 (ru) Способ определени ангиотензина 11
DK16384A (da) Fremgangsmaade til bestemmelse af deoxythymidinkinase (dtk)isoenzymaktivitet
Khalsa Folate and folate binding capacity of human milk.
FR2721931B1 (fr) Conjugues d'analogues de diphosphates ou triphosphates avec des enzymes ou des proteurs antigeniques et dosage immunologique utilisant de tels conjugues

Legal Events

Date Code Title Description
0001 Granted
TA Fee payment date (situation as of event date), data collected since 19931001

Effective date: 19970527