IT1263263B - Metodo di rivelazione multipunto per elettroforesi e cromatografia in capillari - Google Patents

Metodo di rivelazione multipunto per elettroforesi e cromatografia in capillari

Info

Publication number
IT1263263B
IT1263263B ITRM920713A ITRM920713A IT1263263B IT 1263263 B IT1263263 B IT 1263263B IT RM920713 A ITRM920713 A IT RM920713A IT RM920713 A ITRM920713 A IT RM920713A IT 1263263 B IT1263263 B IT 1263263B
Authority
IT
Italy
Prior art keywords
capillary
development
electrophoresis
developer
multipoint method
Prior art date
Application number
ITRM920713A
Other languages
English (en)
Inventor
Stellan Hjterten
Tasanee Srichaiyo
Original Assignee
Bio Rad Laboratories
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bio Rad Laboratories filed Critical Bio Rad Laboratories
Publication of ITRM920713A0 publication Critical patent/ITRM920713A0/it
Publication of ITRM920713A1 publication Critical patent/ITRM920713A1/it
Application granted granted Critical
Publication of IT1263263B publication Critical patent/IT1263263B/it

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/62—Detectors specially adapted therefor
    • G01N30/74—Optical detectors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
    • G01N27/44721—Arrangements for investigating the separated zones, e.g. localising zones by optical means
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G01N21/03—Cuvette constructions
    • G01N21/05—Flow-through cuvettes
    • G01N2021/052—Tubular type; cavity type; multireflective
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/60—Construction of the column
    • G01N30/6004—Construction of the column end pieces
    • G01N2030/6013—Construction of the column end pieces interfaces to detectors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/60—Construction of the column
    • G01N30/6052—Construction of the column body
    • G01N30/6082—Construction of the column body transparent to radiation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/86—Signal analysis
    • G01N30/8603—Signal analysis with integration or differentiation
    • G01N30/8606—Integration

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Electrochemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

Elettroferogrammi e cromatogrammi, multipli rappresentanti successivi stadi di una separazione elettroforetica o cromatografica in un capillare vengono ottenuti dirigendo un registratore su una successione di finestre di rivelazione nel capillare, distanziate lungo la lunghezza del capillare. Viene impiegato un unico rivelatore immobile ed il capillare viene tenuto in modo che ciascuna finestra di rivelazione sia posta in allineamento con il rivelatore in successione, oppure avvolto in modo che le finestre di rivelazione si trovino tutte allo stesso tempo nel percorso del rivelatore.
ITRM920713A 1991-09-30 1992-09-29 Metodo di rivelazione multipunto per elettroforesi e cromatografia in capillari IT1263263B (it)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US07/769,073 US5114551A (en) 1991-09-30 1991-09-30 Multi-point detection method for electrophoresis and chromatography in capillaries

Publications (3)

Publication Number Publication Date
ITRM920713A0 ITRM920713A0 (it) 1992-09-29
ITRM920713A1 ITRM920713A1 (it) 1994-03-29
IT1263263B true IT1263263B (it) 1996-08-05

Family

ID=25084365

Family Applications (1)

Application Number Title Priority Date Filing Date
ITRM920713A IT1263263B (it) 1991-09-30 1992-09-29 Metodo di rivelazione multipunto per elettroforesi e cromatografia in capillari

Country Status (6)

Country Link
US (1) US5114551A (it)
JP (1) JPH0820415B2 (it)
DE (1) DE4230837A1 (it)
FR (1) FR2681944B1 (it)
GB (1) GB2259980B (it)
IT (1) IT1263263B (it)

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US7081226B1 (en) 1996-06-04 2006-07-25 University Of Utah Research Foundation System and method for fluorescence monitoring
US7273749B1 (en) 1990-06-04 2007-09-25 University Of Utah Research Foundation Container for carrying out and monitoring biological processes
US5935522A (en) * 1990-06-04 1999-08-10 University Of Utah Research Foundation On-line DNA analysis system with rapid thermal cycling
US5324401A (en) * 1993-02-05 1994-06-28 Iowa State University Research Foundation, Inc. Multiplexed fluorescence detector system for capillary electrophoresis
US5439578A (en) * 1993-06-03 1995-08-08 The Governors Of The University Of Alberta Multiple capillary biochemical analyzer
GB9509410D0 (en) * 1995-05-10 1995-07-05 Imperial College Molecular imaging
US5582705A (en) * 1995-05-19 1996-12-10 Iowa State University Research Foundation, Inc. Multiplexed capillary electrophoresis system
US5650846A (en) * 1995-11-21 1997-07-22 Hewlett-Packard Company Microcolumnar analytical system with optical fiber sensor
JP4540754B2 (ja) * 1996-06-04 2010-09-08 ユニバーシティ オブ ユタ リサーチ ファウンデーション Ｐｃｒ中のハイブリダイゼーションのモニタリング
JP3239291B2 (ja) * 1996-10-24 2001-12-17 三菱マテリアル株式会社 連続流れ分析法による複数成分の同時分析法とその装置
US6445448B1 (en) 1997-03-12 2002-09-03 Corning Applied Technologies, Corp. System and method for molecular sample measurement
US5903348A (en) * 1997-03-12 1999-05-11 Nz Applied Technologies, Inc. System and method for molecular sample measurements
US5935430A (en) * 1997-04-30 1999-08-10 Hewlett-Packard Company Structure for capturing express transient liquid phase during diffusion bonding of planar devices
US5792943A (en) * 1997-04-30 1998-08-11 Hewlett-Packard Company Planar separation column for use in sample analysis system
US6524866B1 (en) * 1997-12-24 2003-02-25 Cetek Corporation Capillary electrophoretic method to detect target-binding ligands and to determine their relative affinities
US6132968A (en) * 1998-05-13 2000-10-17 University Of Alberta Methods for quantitating low level modifications of nucleotide sequences
US6432651B1 (en) 1998-07-10 2002-08-13 Cetek Corporation Method to detect and analyze tight-binding ligands in complex biological samples using capillary electrophoresis and mass spectrometry
GB0019500D0 (en) * 2000-08-08 2000-09-27 Diamond Optical Tech Ltd System and method
GB0019496D0 (en) * 2000-08-08 2000-09-27 Diamond Optical Tech Ltd System and method
GB0019499D0 (en) * 2000-08-08 2000-09-27 Diamond Optical Tech Ltd system and method
WO2003014324A2 (en) * 2001-08-10 2003-02-20 Board Of Regents, The University Of Texas System Thermostable uvra and uvrb polipeptides and methods of use
US20050002923A1 (en) * 2001-08-10 2005-01-06 Bennet Van Houten Thermostable uvra, uvrb, and uvrc polypeptides and methods of use
US20080288178A1 (en) * 2001-08-24 2008-11-20 Applera Corporation Sequencing system with memory
US7019831B2 (en) * 2001-08-24 2006-03-28 Applera Corporation Separation device substrate including non-fluorescent quencher dye
US6627433B2 (en) 2001-08-24 2003-09-30 Applera Corporation Multi-channel analyte-separation device employing side-entry excitation
US8968560B2 (en) * 2007-12-06 2015-03-03 Schlumberger Technology Corporation Chromatography using multiple detectors
WO2015048769A1 (en) * 2013-09-30 2015-04-02 Board Of Regents, The University Of Texas System Method and apparatus for scanning detection
CN110923782B (zh) * 2019-12-09 2021-04-16 江苏舜天新盈轻工业有限公司 电泳工艺用龙门行车控制装置及其控制系统

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3432414A (en) * 1965-04-01 1969-03-11 Bausch & Lomb Electrophoretic process with continuous scanning
DE1800993A1 (de) * 1968-10-03 1970-05-27 Max Planck Gesellschaft Verfahren zur automatisierten elektronischen densitometrischen Auswertung von mit Hilfe der sogenannten traegerfreien Elektrophorese getrennten Stoffgemischen
US3676649A (en) * 1970-05-08 1972-07-11 Phillips Petroleum Co Signal analysis and recording
LU61023A1 (it) * 1970-05-29 1971-08-12
US4353242A (en) * 1980-12-16 1982-10-12 University Of Utah Research Foundation Multichannel detection and resolution of chromatographic peaks
US4375163A (en) * 1981-01-08 1983-03-01 Varian Associates, Inc. Method and apparatus for on-column detection in liquid chromatography
US4592842A (en) * 1984-03-08 1986-06-03 Spectra-Physics, Inc. Method and apparatus for optimizing peak detection in a chromatogram
US4909920A (en) * 1987-03-16 1990-03-20 Helena Laboratories Automatic electrophoresis apparatus and method
US4859301A (en) * 1988-08-30 1989-08-22 Beckman Instruments, Inc. Separation of zone formation from electroosmotic impulse in tubular electrophoretic systems
US4985129A (en) * 1988-12-02 1991-01-15 Bio-Rad Laboratories, Inc. Apparatus for capillary electrophoresis
JPH0560728A (ja) * 1991-09-05 1993-03-12 Hitachi Ltd 細溝型電気泳動装置

Also Published As

Publication number Publication date
GB2259980A (en) 1993-03-31
GB9220057D0 (en) 1992-11-04
GB2259980B (en) 1995-04-05
ITRM920713A0 (it) 1992-09-29
JPH0820415B2 (ja) 1996-03-04
FR2681944A1 (fr) 1993-04-02
US5114551A (en) 1992-05-19
ITRM920713A1 (it) 1994-03-29
JPH05203622A (ja) 1993-08-10
DE4230837A1 (de) 1993-04-01
FR2681944B1 (fr) 1995-12-29

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