JP2017537645A - 細胞の選択された部分集団中の複数エピトープを識別するための方法 - Google Patents

細胞の選択された部分集団中の複数エピトープを識別するための方法 Download PDF

Info

Publication number
JP2017537645A
JP2017537645A JP2017532096A JP2017532096A JP2017537645A JP 2017537645 A JP2017537645 A JP 2017537645A JP 2017532096 A JP2017532096 A JP 2017532096A JP 2017532096 A JP2017532096 A JP 2017532096A JP 2017537645 A JP2017537645 A JP 2017537645A
Authority
JP
Japan
Prior art keywords
target
sequence
barcode
oligonucleotide
cells
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP2017532096A
Other languages
English (en)
Japanese (ja)
Other versions
JP2017537645A5 (2
Inventor
ゲイリー ピー． ノーラン，
ゲイリー ピー． ノーラン，
Original Assignee
アプライズ バイオ， インコーポレイテッド
アプライズ バイオ， インコーポレイテッド
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by アプライズ バイオ， インコーポレイテッド, アプライズ バイオ， インコーポレイテッド filed Critical アプライズ バイオ， インコーポレイテッド
Publication of JP2017537645A publication Critical patent/JP2017537645A/ja
Publication of JP2017537645A5 publication Critical patent/JP2017537645A5/ja
Pending legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/702—Specific hybridization probes for retroviruses
    • C12Q1/703—Viruses associated with AIDS
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6804—Nucleic acid analysis using immunogens
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56966—Animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2458/00—Labels used in chemical analysis of biological material
    • G01N2458/10—Oligonucleotides as tagging agents for labelling antibodies

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Immunology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Virology (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Cell Biology (AREA)
  • Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Food Science & Technology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • AIDS & HIV (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
JP2017532096A 2014-12-19 2015-12-21 細胞の選択された部分集団中の複数エピトープを識別するための方法 Pending JP2017537645A (ja)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US201462094917P 2014-12-19 2014-12-19
US201462094924P 2014-12-19 2014-12-19
US201462094919P 2014-12-19 2014-12-19
US62/094,919 2014-12-19
US62/094,917 2014-12-19
US62/094,924 2014-12-19
PCT/US2015/067147 WO2016100976A2 (en) 2014-12-19 2015-12-21 Methods for identifying multiple epitopes in selected sub-populations of cells

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2020186442A Division JP7196143B2 (ja) 2014-12-19 2020-11-09 細胞の選択された部分集団中の複数エピトープを識別するための方法

Publications (2)

Publication Number Publication Date
JP2017537645A true JP2017537645A (ja) 2017-12-21
JP2017537645A5 JP2017537645A5 (2) 2019-01-17

Family

ID=56127888

Family Applications (2)

Application Number Title Priority Date Filing Date
JP2017532096A Pending JP2017537645A (ja) 2014-12-19 2015-12-21 細胞の選択された部分集団中の複数エピトープを識別するための方法
JP2020186442A Active JP7196143B2 (ja) 2014-12-19 2020-11-09 細胞の選択された部分集団中の複数エピトープを識別するための方法

Family Applications After (1)

Application Number Title Priority Date Filing Date
JP2020186442A Active JP7196143B2 (ja) 2014-12-19 2020-11-09 細胞の選択された部分集団中の複数エピトープを識別するための方法

Country Status (5)

Country Link
US (3) US20180320241A1 (2)
EP (1) EP3234602B1 (2)
JP (2) JP2017537645A (2)
CN (1) CN107110849B (2)
WO (1) WO2016100976A2 (2)

Families Citing this family (81)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
WO2012106385A2 (en) 2011-01-31 2012-08-09 Apprise Bio, Inc. Methods of identifying multiple epitopes in cells
US11560585B2 (en) 2011-01-31 2023-01-24 Roche Sequencing Solutions, Inc. Methods of identifying multiple epitopes in cells
CA2865575C (en) 2012-02-27 2024-01-16 Cellular Research, Inc. Compositions and kits for molecular counting
AU2013302756C1 (en) 2012-08-14 2018-05-17 10X Genomics, Inc. Microcapsule compositions and methods
US9951386B2 (en) 2014-06-26 2018-04-24 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10584381B2 (en) 2012-08-14 2020-03-10 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9701998B2 (en) 2012-12-14 2017-07-11 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10752949B2 (en) 2012-08-14 2020-08-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US11591637B2 (en) 2012-08-14 2023-02-28 10X Genomics, Inc. Compositions and methods for sample processing
US10323279B2 (en) 2012-08-14 2019-06-18 10X Genomics, Inc. Methods and systems for processing polynucleotides
CA2894694C (en) 2012-12-14 2023-04-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10533221B2 (en) 2012-12-14 2020-01-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
KR20200140929A (ko) 2013-02-08 2020-12-16 10엑스 제노믹스, 인크. 폴리뉴클레오티드 바코드 생성
KR102758333B1 (ko) 2013-08-28 2025-01-23 벡톤 디킨슨 앤드 컴퍼니 대량의 동시 단일 세포 분석
BR112016023625A2 (pt) 2014-04-10 2018-06-26 10X Genomics, Inc. dispositivos fluídicos, sistemas e métodos para encapsular e particionar reagentes, e aplicações dos mesmos
US12312640B2 (en) 2014-06-26 2025-05-27 10X Genomics, Inc. Analysis of nucleic acid sequences
WO2015200893A2 (en) 2014-06-26 2015-12-30 10X Genomics, Inc. Methods of analyzing nucleic acids from individual cells or cell populations
CA2953469A1 (en) 2014-06-26 2015-12-30 10X Genomics, Inc. Analysis of nucleic acid sequences
US10900065B2 (en) 2014-11-14 2021-01-26 University Of Washington Methods and kits for labeling cellular molecules
JP6769969B2 (ja) 2015-01-12 2020-10-14 １０エックス ジェノミクス， インコーポレイテッド 核酸配列決定ライブラリーを作製するためのプロセス及びシステム、並びにこれらを使用して作製したライブラリー
EP3262407B1 (en) 2015-02-24 2023-08-30 10X Genomics, Inc. Partition processing methods and systems
WO2016138496A1 (en) 2015-02-27 2016-09-01 Cellular Research, Inc. Spatially addressable molecular barcoding
WO2016160844A2 (en) 2015-03-30 2016-10-06 Cellular Research, Inc. Methods and compositions for combinatorial barcoding
EP4582556A3 (en) 2015-04-23 2025-10-08 Becton, Dickinson and Company Methods and compositions for whole transcriptome amplification
JP6940484B2 (ja) 2015-09-11 2021-09-29 セルラー リサーチ， インコーポレイテッド ライブラリー正規化のための方法および組成物
US10301677B2 (en) 2016-05-25 2019-05-28 Cellular Research, Inc. Normalization of nucleic acid libraries
US10202641B2 (en) 2016-05-31 2019-02-12 Cellular Research, Inc. Error correction in amplification of samples
US10640763B2 (en) 2016-05-31 2020-05-05 Cellular Research, Inc. Molecular indexing of internal sequences
EP4717780A2 (en) 2016-07-22 2026-04-01 Verily Life Sciences LLC Quantitative massively parallel proteomics
CN109791157B (zh) 2016-09-26 2022-06-07 贝克顿迪金森公司 使用具有条形码化的寡核苷酸序列的试剂测量蛋白质表达
US10550429B2 (en) 2016-12-22 2020-02-04 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10815525B2 (en) 2016-12-22 2020-10-27 10X Genomics, Inc. Methods and systems for processing polynucleotides
GB201622222D0 (en) * 2016-12-23 2017-02-08 Cs Genetics Ltd Reagents and methods for molecular barcoding of nucleic acids of single cells
CN110291207A (zh) * 2017-01-27 2019-09-27 豪夫迈·罗氏有限公司 用于长程测序的条形码化dna
EP4029939B1 (en) 2017-01-30 2023-06-28 10X Genomics, Inc. Methods and systems for droplet-based single cell barcoding
CN110382708A (zh) 2017-02-01 2019-10-25 赛卢拉研究公司 使用阻断性寡核苷酸进行选择性扩增
US11072816B2 (en) 2017-05-03 2021-07-27 The Broad Institute, Inc. Single-cell proteomic assay using aptamers
US10400235B2 (en) 2017-05-26 2019-09-03 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
EP3445876B1 (en) 2017-05-26 2023-07-05 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
CN110719959B (zh) 2017-06-05 2021-08-06 贝克顿迪金森公司 针对单细胞的样品索引
EP3447152A1 (en) * 2017-08-25 2019-02-27 Eawag, Swiss Federal Institute of Aquatic Science and Technology Accurate and massively parallel quantification of nucleic acid
ES2994431T3 (en) 2017-09-22 2025-01-23 Univ Washington In situ combinatorial labeling of cellular molecules
SG11201913654QA (en) 2017-11-15 2020-01-30 10X Genomics Inc Functionalized gel beads
US10829815B2 (en) 2017-11-17 2020-11-10 10X Genomics, Inc. Methods and systems for associating physical and genetic properties of biological particles
EP3568494B1 (en) * 2017-12-08 2021-05-05 10X Genomics, Inc. Methods and compositions for labeling cells
US11639928B2 (en) 2018-02-22 2023-05-02 10X Genomics, Inc. Methods and systems for characterizing analytes from individual cells or cell populations
US11841371B2 (en) 2018-03-13 2023-12-12 The Broad Institute, Inc. Proteomics and spatial patterning using antenna networks
CN112262218B (zh) 2018-04-06 2024-11-08 10X基因组学有限公司 用于单细胞处理中的质量控制的系统和方法
CN112272710A (zh) 2018-05-03 2021-01-26 贝克顿迪金森公司 高通量多组学样品分析
JP7358388B2 (ja) 2018-05-03 2023-10-10 ベクトン・ディキンソン・アンド・カンパニー 反対側の転写物末端における分子バーコーディング
US11039856B2 (en) 2018-05-10 2021-06-22 Verily Life Sciences Llc Surgical laparoscopic ports for implanting medical systems
US11414701B2 (en) 2018-05-24 2022-08-16 The Broad Institute, Inc. Multimodal readouts for quantifying and sequencing nucleic acids in single cells
ES2992135T3 (es) 2018-10-01 2024-12-09 Becton Dickinson Co Determinar secuencias de transcripción 5’
US11932849B2 (en) 2018-11-08 2024-03-19 Becton, Dickinson And Company Whole transcriptome analysis of single cells using random priming
JP2022513124A (ja) * 2018-11-21 2022-02-07 アヴィダ バイオメッド， インコーポレイテッド 標的化された核酸ライブラリー形成のための方法
JP7332694B2 (ja) * 2018-12-04 2023-08-23 エフ． ホフマン－ラ ロシュ アーゲー 細胞標的の空間指向性量子バーコード化
US11492660B2 (en) 2018-12-13 2022-11-08 Becton, Dickinson And Company Selective extension in single cell whole transcriptome analysis
ES2945227T3 (es) 2019-01-23 2023-06-29 Becton Dickinson Co Oligonucleótidos asociados con anticuerpos
WO2020167920A1 (en) 2019-02-14 2020-08-20 Cellular Research, Inc. Hybrid targeted and whole transcriptome amplification
SG10201904897UA (en) * 2019-05-30 2020-12-30 Nat Univ Singapore Method, structures and system for nucleic acid sequence topology assembly for multiplexed profiling of proteins.
CN120099137A (zh) 2019-07-22 2025-06-06 贝克顿迪金森公司 单细胞染色质免疫沉淀测序测定
WO2021034791A1 (en) * 2019-08-19 2021-02-25 Inscripta, Inc. Optimizing high-throughput sequencing capacity
JP7522189B2 (ja) 2019-11-08 2024-07-24 ベクトン・ディキンソン・アンド・カンパニー 免疫レパートリーシーケンシングのための完全長ｖ（ｄ）ｊ情報を得るためのランダムプライミングの使用
US12163189B2 (en) 2019-11-27 2024-12-10 University Of Washington Method for preparation and high-throughput microbial single-cell RNA sequencing of bacteria
EP4069731A4 (en) * 2019-12-03 2024-05-29 Alamar Biosciences, Inc. Nucleic acid linked immune-sandwich assay (nulisa)
WO2021146219A1 (en) 2020-01-13 2021-07-22 Becton, Dickinson And Company Cell capture using du-containing oligonucleotides
US11649497B2 (en) 2020-01-13 2023-05-16 Becton, Dickinson And Company Methods and compositions for quantitation of proteins and RNA
WO2021146393A1 (en) * 2020-01-14 2021-07-22 William Marsh Rice University High throughput genetic barcoding and analysis methods
WO2021155057A1 (en) 2020-01-29 2021-08-05 Becton, Dickinson And Company Barcoded wells for spatial mapping of single cells through sequencing
WO2021173719A1 (en) 2020-02-25 2021-09-02 Becton, Dickinson And Company Bi-specific probes to enable the use of single-cell samples as single color compensation control
US11661625B2 (en) 2020-05-14 2023-05-30 Becton, Dickinson And Company Primers for immune repertoire profiling
US12157913B2 (en) 2020-06-02 2024-12-03 Becton, Dickinson And Company Oligonucleotides and beads for 5 prime gene expression assay
EP4162069A1 (en) * 2020-06-08 2023-04-12 F. Hoffmann-La Roche AG Method of single-cell analysis of multiple samples
WO2022008649A1 (en) * 2020-07-08 2022-01-13 F. Hoffmann-La Roche Ag Spatial analysis of multiple targets in tissue samples
US11932901B2 (en) 2020-07-13 2024-03-19 Becton, Dickinson And Company Target enrichment using nucleic acid probes for scRNAseq
US12391940B2 (en) 2020-07-31 2025-08-19 Becton, Dickinson And Company Single cell assay for transposase-accessible chromatin
EP4247967A1 (en) 2020-11-20 2023-09-27 Becton, Dickinson and Company Profiling of highly expressed and lowly expressed proteins
CN116685850A (zh) 2020-12-15 2023-09-01 贝克顿迪金森公司 单细胞分泌组分析
CN117015617B (zh) 2021-02-23 2025-04-04 10X基因组学有限公司 基于探针的核酸和蛋白质分析
EP4399327B1 (en) * 2021-09-08 2025-10-29 Becton, Dickinson and Company Non-sequencing pcr-based method for detection of antibody-conjugated oligonucleotides

Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2003524419A (ja) * 2000-02-18 2003-08-19 ウルフ・ランデグレン 近接プロービング方法およびキット
WO2005123963A2 (en) * 2004-06-14 2005-12-29 The Board Of Trustees Of The Leland Stanford Junior University Methods and compositions for use in analyte detection using proximity probes
US20100203543A1 (en) * 2007-08-10 2010-08-12 Peter Oliver Krutzik Biological encoding of large numbers of cells
US20100240101A1 (en) * 2009-03-19 2010-09-23 Massachusetts Institute Of Technology Parallel Proximity Ligation Event Analysis
JP2012502625A (ja) * 2008-09-16 2012-02-02 ナチュラル エンヴァイロメント リサーチ カウンシル 樹状細胞調節分子
WO2014026032A2 (en) * 2012-08-08 2014-02-13 Apprise Bio, Inc. Increasing dynamic range for identifying multiple epitopes in cells
JP2014507141A (ja) * 2011-02-09 2014-03-27 ナテラ， インコーポレイテッド 非侵襲的出生前倍数性呼び出しのための方法
JP2014513916A (ja) * 2011-01-31 2014-06-19 アプライズ バイオ， インコーポレイテッド 細胞において複数のエピトープを同定する方法
WO2014099744A1 (en) * 2012-12-17 2014-06-26 President And Fellows Of Harvard College Rna-guided human genome engineering

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5242794A (en) 1984-12-13 1993-09-07 Applied Biosystems, Inc. Detection of specific sequences in nucleic acids
US4988617A (en) 1988-03-25 1991-01-29 California Institute Of Technology Method of detecting a nucleotide change in nucleic acids
US5494810A (en) 1990-05-03 1996-02-27 Cornell Research Foundation, Inc. Thermostable ligase-mediated DNA amplifications system for the detection of genetic disease
SE9400088D0 (sv) 1994-01-14 1994-01-14 Kabi Pharmacia Ab Bacterial receptor structures
US6582938B1 (en) 2001-05-11 2003-06-24 Affymetrix, Inc. Amplification of nucleic acids
US6625546B2 (en) * 2000-02-03 2003-09-23 Nanoscale Combinatorial Synthesis, Inc. Structure identification methods using mass measurements
US7473767B2 (en) 2001-07-03 2009-01-06 The Institute For Systems Biology Methods for detection and quantification of analytes in complex mixtures
AU2009234373B2 (en) * 2008-04-09 2015-09-24 Cornell University Coferons and methods of making and using them
US9315857B2 (en) * 2009-12-15 2016-04-19 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse label-tags

Patent Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2003524419A (ja) * 2000-02-18 2003-08-19 ウルフ・ランデグレン 近接プロービング方法およびキット
WO2005123963A2 (en) * 2004-06-14 2005-12-29 The Board Of Trustees Of The Leland Stanford Junior University Methods and compositions for use in analyte detection using proximity probes
US20100203543A1 (en) * 2007-08-10 2010-08-12 Peter Oliver Krutzik Biological encoding of large numbers of cells
JP2012502625A (ja) * 2008-09-16 2012-02-02 ナチュラル エンヴァイロメント リサーチ カウンシル 樹状細胞調節分子
US20100240101A1 (en) * 2009-03-19 2010-09-23 Massachusetts Institute Of Technology Parallel Proximity Ligation Event Analysis
JP2014513916A (ja) * 2011-01-31 2014-06-19 アプライズ バイオ， インコーポレイテッド 細胞において複数のエピトープを同定する方法
JP2014507141A (ja) * 2011-02-09 2014-03-27 ナテラ， インコーポレイテッド 非侵襲的出生前倍数性呼び出しのための方法
WO2014026032A2 (en) * 2012-08-08 2014-02-13 Apprise Bio, Inc. Increasing dynamic range for identifying multiple epitopes in cells
WO2014099744A1 (en) * 2012-12-17 2014-06-26 President And Fellows Of Harvard College Rna-guided human genome engineering

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
NATURE BIOTECHNOLOGY, vol. 20, JPN6015053064, 2002, pages 473 - 477, ISSN: 0004132563 *
NUCLEIC ACIDS RESEARCH, 2005, VOL.33, NO.18 E162，P.1-7, JPN6019039275, ISSN: 0004298051 *

Also Published As

Publication number Publication date
EP3234602A2 (en) 2017-10-25
WO2016100976A2 (en) 2016-06-23
JP7196143B2 (ja) 2022-12-26
CN107110849A (zh) 2017-08-29
WO2016100976A3 (en) 2016-09-22
US20230049314A1 (en) 2023-02-16
EP3234602B1 (en) 2021-08-25
JP2021010388A (ja) 2021-02-04
US20210002733A1 (en) 2021-01-07
CN107110849B (zh) 2019-07-26
US20180320241A1 (en) 2018-11-08
EP3234602A4 (en) 2018-05-30

Similar Documents

Publication Publication Date Title
JP7196143B2 (ja) 細胞の選択された部分集団中の複数エピトープを識別するための方法
US11859240B2 (en) Methods of identifying multiple epitopes in cells
JP7828901B2 (ja) 試料中の異なる分析物及び分析物の異なるサブグループ／変形物を検出するための多重的方法
JP2023154012A (ja) 細胞標的の空間指向性量子バーコード化
JP2022537048A (ja) 試料中の分析物のシグナルコード化方法
JP2025166110A (ja) 核酸結合免疫サンドイッチアッセイ（ｎｕｌｉｓａ）
US20220049286A1 (en) Methods of identifying multiple epitopes in cells
JP2007524410A (ja) ポリヌクレオチドライゲーション反応の改良
US10982271B2 (en) Methods of identifying multiple epitopes in cells
US11560585B2 (en) Methods of identifying multiple epitopes in cells
CN118591637B (zh) 用于蛋白质测量的新一代测序
US20200157603A1 (en) Methods of identifying multiple epitopes in cells
US12203139B2 (en) Systems and methods for detecting multi-molecule biomarkers
EP4728094A1 (en) Reagent for in situ hybridization quality control
KR101782806B1 (ko) 차세대염기서열분석기술 기반의 고효율, 고해상도 조직적합성 형별 분석 방법 및 키트

Legal Events

Date Code Title Description
A711 Notification of change in applicant

Free format text: JAPANESE INTERMEDIATE CODE: A712

Effective date: 20180508

Free format text: JAPANESE INTERMEDIATE CODE: A711

Effective date: 20180508

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20181126

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20181126

A977 Report on retrieval

Free format text: JAPANESE INTERMEDIATE CODE: A971007

Effective date: 20190920

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20191015

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20200114

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20200213

A02 Decision of refusal

Free format text: JAPANESE INTERMEDIATE CODE: A02

Effective date: 20200707