JP2017537645A5 - - Google Patents

Download PDF

Info

Publication number
JP2017537645A5
JP2017537645A5 JP2017532096A JP2017532096A JP2017537645A5 JP 2017537645 A5 JP2017537645 A5 JP 2017537645A5 JP 2017532096 A JP2017532096 A JP 2017532096A JP 2017532096 A JP2017532096 A JP 2017532096A JP 2017537645 A5 JP2017537645 A5 JP 2017537645A5
Authority
JP
Japan
Prior art keywords
barcode
epitope
unique binding
cells
cell origin
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP2017532096A
Other languages
English (en)
Japanese (ja)
Other versions
JP2017537645A (ja
Filing date
Publication date
Application filed filed Critical
Priority claimed from PCT/US2015/067147 external-priority patent/WO2016100976A2/en
Publication of JP2017537645A publication Critical patent/JP2017537645A/ja
Publication of JP2017537645A5 publication Critical patent/JP2017537645A5/ja
Pending legal-status Critical Current

Links

JP2017532096A 2014-12-19 2015-12-21 細胞の選択された部分集団中の複数エピトープを識別するための方法 Pending JP2017537645A (ja)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US201462094917P 2014-12-19 2014-12-19
US201462094924P 2014-12-19 2014-12-19
US201462094919P 2014-12-19 2014-12-19
US62/094,919 2014-12-19
US62/094,917 2014-12-19
US62/094,924 2014-12-19
PCT/US2015/067147 WO2016100976A2 (en) 2014-12-19 2015-12-21 Methods for identifying multiple epitopes in selected sub-populations of cells

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2020186442A Division JP7196143B2 (ja) 2014-12-19 2020-11-09 細胞の選択された部分集団中の複数エピトープを識別するための方法

Publications (2)

Publication Number Publication Date
JP2017537645A JP2017537645A (ja) 2017-12-21
JP2017537645A5 true JP2017537645A5 (2) 2019-01-17

Family

ID=56127888

Family Applications (2)

Application Number Title Priority Date Filing Date
JP2017532096A Pending JP2017537645A (ja) 2014-12-19 2015-12-21 細胞の選択された部分集団中の複数エピトープを識別するための方法
JP2020186442A Active JP7196143B2 (ja) 2014-12-19 2020-11-09 細胞の選択された部分集団中の複数エピトープを識別するための方法

Family Applications After (1)

Application Number Title Priority Date Filing Date
JP2020186442A Active JP7196143B2 (ja) 2014-12-19 2020-11-09 細胞の選択された部分集団中の複数エピトープを識別するための方法

Country Status (5)

Country Link
US (3) US20180320241A1 (2)
EP (1) EP3234602B1 (2)
JP (2) JP2017537645A (2)
CN (1) CN107110849B (2)
WO (1) WO2016100976A2 (2)

Families Citing this family (81)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
WO2012106385A2 (en) 2011-01-31 2012-08-09 Apprise Bio, Inc. Methods of identifying multiple epitopes in cells
US11560585B2 (en) 2011-01-31 2023-01-24 Roche Sequencing Solutions, Inc. Methods of identifying multiple epitopes in cells
CA2865575C (en) 2012-02-27 2024-01-16 Cellular Research, Inc. Compositions and kits for molecular counting
AU2013302756C1 (en) 2012-08-14 2018-05-17 10X Genomics, Inc. Microcapsule compositions and methods
US9951386B2 (en) 2014-06-26 2018-04-24 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10584381B2 (en) 2012-08-14 2020-03-10 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9701998B2 (en) 2012-12-14 2017-07-11 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10752949B2 (en) 2012-08-14 2020-08-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US11591637B2 (en) 2012-08-14 2023-02-28 10X Genomics, Inc. Compositions and methods for sample processing
US10323279B2 (en) 2012-08-14 2019-06-18 10X Genomics, Inc. Methods and systems for processing polynucleotides
CA2894694C (en) 2012-12-14 2023-04-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10533221B2 (en) 2012-12-14 2020-01-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
KR20200140929A (ko) 2013-02-08 2020-12-16 10엑스 제노믹스, 인크. 폴리뉴클레오티드 바코드 생성
KR102758333B1 (ko) 2013-08-28 2025-01-23 벡톤 디킨슨 앤드 컴퍼니 대량의 동시 단일 세포 분석
BR112016023625A2 (pt) 2014-04-10 2018-06-26 10X Genomics, Inc. dispositivos fluídicos, sistemas e métodos para encapsular e particionar reagentes, e aplicações dos mesmos
US12312640B2 (en) 2014-06-26 2025-05-27 10X Genomics, Inc. Analysis of nucleic acid sequences
WO2015200893A2 (en) 2014-06-26 2015-12-30 10X Genomics, Inc. Methods of analyzing nucleic acids from individual cells or cell populations
CA2953469A1 (en) 2014-06-26 2015-12-30 10X Genomics, Inc. Analysis of nucleic acid sequences
US10900065B2 (en) 2014-11-14 2021-01-26 University Of Washington Methods and kits for labeling cellular molecules
JP6769969B2 (ja) 2015-01-12 2020-10-14 １０エックス ジェノミクス， インコーポレイテッド 核酸配列決定ライブラリーを作製するためのプロセス及びシステム、並びにこれらを使用して作製したライブラリー
EP3262407B1 (en) 2015-02-24 2023-08-30 10X Genomics, Inc. Partition processing methods and systems
WO2016138496A1 (en) 2015-02-27 2016-09-01 Cellular Research, Inc. Spatially addressable molecular barcoding
WO2016160844A2 (en) 2015-03-30 2016-10-06 Cellular Research, Inc. Methods and compositions for combinatorial barcoding
EP4582556A3 (en) 2015-04-23 2025-10-08 Becton, Dickinson and Company Methods and compositions for whole transcriptome amplification
JP6940484B2 (ja) 2015-09-11 2021-09-29 セルラー リサーチ， インコーポレイテッド ライブラリー正規化のための方法および組成物
US10301677B2 (en) 2016-05-25 2019-05-28 Cellular Research, Inc. Normalization of nucleic acid libraries
US10202641B2 (en) 2016-05-31 2019-02-12 Cellular Research, Inc. Error correction in amplification of samples
US10640763B2 (en) 2016-05-31 2020-05-05 Cellular Research, Inc. Molecular indexing of internal sequences
EP4717780A2 (en) 2016-07-22 2026-04-01 Verily Life Sciences LLC Quantitative massively parallel proteomics
CN109791157B (zh) 2016-09-26 2022-06-07 贝克顿迪金森公司 使用具有条形码化的寡核苷酸序列的试剂测量蛋白质表达
US10550429B2 (en) 2016-12-22 2020-02-04 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10815525B2 (en) 2016-12-22 2020-10-27 10X Genomics, Inc. Methods and systems for processing polynucleotides
GB201622222D0 (en) * 2016-12-23 2017-02-08 Cs Genetics Ltd Reagents and methods for molecular barcoding of nucleic acids of single cells
CN110291207A (zh) * 2017-01-27 2019-09-27 豪夫迈·罗氏有限公司 用于长程测序的条形码化dna
EP4029939B1 (en) 2017-01-30 2023-06-28 10X Genomics, Inc. Methods and systems for droplet-based single cell barcoding
CN110382708A (zh) 2017-02-01 2019-10-25 赛卢拉研究公司 使用阻断性寡核苷酸进行选择性扩增
US11072816B2 (en) 2017-05-03 2021-07-27 The Broad Institute, Inc. Single-cell proteomic assay using aptamers
US10400235B2 (en) 2017-05-26 2019-09-03 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
EP3445876B1 (en) 2017-05-26 2023-07-05 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
CN110719959B (zh) 2017-06-05 2021-08-06 贝克顿迪金森公司 针对单细胞的样品索引
EP3447152A1 (en) * 2017-08-25 2019-02-27 Eawag, Swiss Federal Institute of Aquatic Science and Technology Accurate and massively parallel quantification of nucleic acid
ES2994431T3 (en) 2017-09-22 2025-01-23 Univ Washington In situ combinatorial labeling of cellular molecules
SG11201913654QA (en) 2017-11-15 2020-01-30 10X Genomics Inc Functionalized gel beads
US10829815B2 (en) 2017-11-17 2020-11-10 10X Genomics, Inc. Methods and systems for associating physical and genetic properties of biological particles
EP3568494B1 (en) * 2017-12-08 2021-05-05 10X Genomics, Inc. Methods and compositions for labeling cells
US11639928B2 (en) 2018-02-22 2023-05-02 10X Genomics, Inc. Methods and systems for characterizing analytes from individual cells or cell populations
US11841371B2 (en) 2018-03-13 2023-12-12 The Broad Institute, Inc. Proteomics and spatial patterning using antenna networks
CN112262218B (zh) 2018-04-06 2024-11-08 10X基因组学有限公司 用于单细胞处理中的质量控制的系统和方法
CN112272710A (zh) 2018-05-03 2021-01-26 贝克顿迪金森公司 高通量多组学样品分析
JP7358388B2 (ja) 2018-05-03 2023-10-10 ベクトン・ディキンソン・アンド・カンパニー 反対側の転写物末端における分子バーコーディング
US11039856B2 (en) 2018-05-10 2021-06-22 Verily Life Sciences Llc Surgical laparoscopic ports for implanting medical systems
US11414701B2 (en) 2018-05-24 2022-08-16 The Broad Institute, Inc. Multimodal readouts for quantifying and sequencing nucleic acids in single cells
ES2992135T3 (es) 2018-10-01 2024-12-09 Becton Dickinson Co Determinar secuencias de transcripción 5’
US11932849B2 (en) 2018-11-08 2024-03-19 Becton, Dickinson And Company Whole transcriptome analysis of single cells using random priming
JP2022513124A (ja) * 2018-11-21 2022-02-07 アヴィダ バイオメッド， インコーポレイテッド 標的化された核酸ライブラリー形成のための方法
JP7332694B2 (ja) * 2018-12-04 2023-08-23 エフ． ホフマン－ラ ロシュ アーゲー 細胞標的の空間指向性量子バーコード化
US11492660B2 (en) 2018-12-13 2022-11-08 Becton, Dickinson And Company Selective extension in single cell whole transcriptome analysis
ES2945227T3 (es) 2019-01-23 2023-06-29 Becton Dickinson Co Oligonucleótidos asociados con anticuerpos
WO2020167920A1 (en) 2019-02-14 2020-08-20 Cellular Research, Inc. Hybrid targeted and whole transcriptome amplification
SG10201904897UA (en) * 2019-05-30 2020-12-30 Nat Univ Singapore Method, structures and system for nucleic acid sequence topology assembly for multiplexed profiling of proteins.
CN120099137A (zh) 2019-07-22 2025-06-06 贝克顿迪金森公司 单细胞染色质免疫沉淀测序测定
WO2021034791A1 (en) * 2019-08-19 2021-02-25 Inscripta, Inc. Optimizing high-throughput sequencing capacity
JP7522189B2 (ja) 2019-11-08 2024-07-24 ベクトン・ディキンソン・アンド・カンパニー 免疫レパートリーシーケンシングのための完全長ｖ（ｄ）ｊ情報を得るためのランダムプライミングの使用
US12163189B2 (en) 2019-11-27 2024-12-10 University Of Washington Method for preparation and high-throughput microbial single-cell RNA sequencing of bacteria
EP4069731A4 (en) * 2019-12-03 2024-05-29 Alamar Biosciences, Inc. Nucleic acid linked immune-sandwich assay (nulisa)
WO2021146219A1 (en) 2020-01-13 2021-07-22 Becton, Dickinson And Company Cell capture using du-containing oligonucleotides
US11649497B2 (en) 2020-01-13 2023-05-16 Becton, Dickinson And Company Methods and compositions for quantitation of proteins and RNA
WO2021146393A1 (en) * 2020-01-14 2021-07-22 William Marsh Rice University High throughput genetic barcoding and analysis methods
WO2021155057A1 (en) 2020-01-29 2021-08-05 Becton, Dickinson And Company Barcoded wells for spatial mapping of single cells through sequencing
WO2021173719A1 (en) 2020-02-25 2021-09-02 Becton, Dickinson And Company Bi-specific probes to enable the use of single-cell samples as single color compensation control
US11661625B2 (en) 2020-05-14 2023-05-30 Becton, Dickinson And Company Primers for immune repertoire profiling
US12157913B2 (en) 2020-06-02 2024-12-03 Becton, Dickinson And Company Oligonucleotides and beads for 5 prime gene expression assay
EP4162069A1 (en) * 2020-06-08 2023-04-12 F. Hoffmann-La Roche AG Method of single-cell analysis of multiple samples
WO2022008649A1 (en) * 2020-07-08 2022-01-13 F. Hoffmann-La Roche Ag Spatial analysis of multiple targets in tissue samples
US11932901B2 (en) 2020-07-13 2024-03-19 Becton, Dickinson And Company Target enrichment using nucleic acid probes for scRNAseq
US12391940B2 (en) 2020-07-31 2025-08-19 Becton, Dickinson And Company Single cell assay for transposase-accessible chromatin
EP4247967A1 (en) 2020-11-20 2023-09-27 Becton, Dickinson and Company Profiling of highly expressed and lowly expressed proteins
CN116685850A (zh) 2020-12-15 2023-09-01 贝克顿迪金森公司 单细胞分泌组分析
CN117015617B (zh) 2021-02-23 2025-04-04 10X基因组学有限公司 基于探针的核酸和蛋白质分析
EP4399327B1 (en) * 2021-09-08 2025-10-29 Becton, Dickinson and Company Non-sequencing pcr-based method for detection of antibody-conjugated oligonucleotides

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5242794A (en) 1984-12-13 1993-09-07 Applied Biosystems, Inc. Detection of specific sequences in nucleic acids
US4988617A (en) 1988-03-25 1991-01-29 California Institute Of Technology Method of detecting a nucleotide change in nucleic acids
US5494810A (en) 1990-05-03 1996-02-27 Cornell Research Foundation, Inc. Thermostable ligase-mediated DNA amplifications system for the detection of genetic disease
SE9400088D0 (sv) 1994-01-14 1994-01-14 Kabi Pharmacia Ab Bacterial receptor structures
US6582938B1 (en) 2001-05-11 2003-06-24 Affymetrix, Inc. Amplification of nucleic acids
US6625546B2 (en) * 2000-02-03 2003-09-23 Nanoscale Combinatorial Synthesis, Inc. Structure identification methods using mass measurements
SE516272C2 (sv) * 2000-02-18 2001-12-10 Ulf Landegren Metoder och kit för analytdetektion mha proximitets-probning
US7473767B2 (en) 2001-07-03 2009-01-06 The Institute For Systems Biology Methods for detection and quantification of analytes in complex mixtures
US7914987B2 (en) * 2004-06-14 2011-03-29 The Board Of Trustees Of The Leland Stanford Junior University Methods and compositions for use in analyte detection using proximity probes
WO2009023107A1 (en) * 2007-08-10 2009-02-19 Peter Oliver Krutzik Biological encoding of large numbers of cells
AU2009234373B2 (en) * 2008-04-09 2015-09-24 Cornell University Coferons and methods of making and using them
GB0816976D0 (en) * 2008-09-16 2008-10-22 Natural Environment Res Council Protein
US8481698B2 (en) * 2009-03-19 2013-07-09 The President And Fellows Of Harvard College Parallel proximity ligation event analysis
US9315857B2 (en) * 2009-12-15 2016-04-19 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse label-tags
WO2012106385A2 (en) * 2011-01-31 2012-08-09 Apprise Bio, Inc. Methods of identifying multiple epitopes in cells
RU2671980C2 (ru) * 2011-02-09 2018-11-08 Натера, Инк. Способы неинвазивного пренатального установления плоидности
EP2882868B1 (en) * 2012-08-08 2019-07-31 H. Hoffnabb-La Roche Ag Increasing dynamic range for identifying multiple epitopes in cells
DK2931891T3 (da) * 2012-12-17 2019-08-19 Harvard College Rna-styret modificering af menneskelige genomer

Similar Documents

Publication Publication Date Title
JP2017537645A5 (2)
US10982257B2 (en) Methods to profile molecular complexes or single cells via proximity dependent barcoding
Song et al. Single cell transcriptomics: moving towards multi-omics
CN111032881B (zh) 核酸的精确和大规模平行定量
KR102914478B1 (ko) 어레이 상에서 인덱스 및 바코드를 사용하여 리간드를 확인하기 위한 방법 및 조성물
JP2019518434A5 (2)
JP2020511629A5 (2)
US9850482B2 (en) Heterologous DNA barcoding method
JP2018529314A5 (2)
JP2019520037A5 (2)
JP2018516541A5 (2)
WO2012159564A1 (zh) 甲基化高通量检测方法
US10435736B2 (en) Target region enrichment method based on multiplex PCR, and reagent
ES3013064T3 (en) Methods for accurate parallel detection and quantification of nucleic acids
JP2022502343A (ja) 肝癌によく見られた複数の変異を同時に検出するｃｔＤＮＡライブラリーの構築及びシークエンシングデータの分析の方法
CN106498504A (zh) 基于多重pcr的二代测序建库技术
ES2942546T3 (es) Métodos de alta sensibilidad para la cuantificación paralela precisa de ácidos nucleicos
CN113557300A (zh) 核酸序列、rna目标区域测序文库的构建方法及应用
CN108823296B (zh) 一种检测核酸样本污染的方法、试剂盒及应用
JP2009502190A5 (2)
JP7762690B2 (ja) 変異核酸の正確な並行定量するための高感度方法
US9499814B2 (en) Methods for identifying drug effects on a cell by determining changes in the cell's spliced message profile
Tost et al. Molecular techniques for DNA methylation studies
FI4077713T3 (fi) Menetelmä ja kitti kokogenomiamplifikaatioon ja biologisessa näytteessä olevien kohdemolekyylien analysointiin
US9845507B2 (en) Methods for detecting oncogenic mutations