JP2995505B2 - How to stabilize polysaccharides - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a method for stabilizing an aqueous solution containing an acidic polysaccharide produced by a callus of a plant belonging to the genus Polyanthes.

[0002]

2. Description of the Related Art In general, external preparations and cosmetics in the form of aqueous solutions, emulsions, gels and the like having a suitable viscosity have been provided because of their good feel upon use. Various polymer substances are used as the agent.

[0003] Also, polysaccharides derived from animals and plants have excellent thickening and gelling effects, and are thickeners, gelling agents, foam stabilizers, suspension / emulsion stabilizers, film-forming agents, adhesives, Widely used as agents and bioactive agents.

[0004] However, there is a problem that it is generally difficult to maintain the viscosity of an aqueous solution containing a polysaccharide for a long period of time. Therefore, for example, in the case of hyaluronic acid, which is an animal-derived polysaccharide, the viscosity is stabilized by adding a polymer such as polyacrylic acid or a polyamino acid, or by adding a compound having a phenolic hydroxyl group. (JP-A-57-185208 and JP-A-1-113401). However, satisfactory results have not yet been obtained by this method.

On the other hand, by blending an acidic polysaccharide produced by a callus of a plant belonging to the genus Polyanthes into a detergent composition or an external preparation composition, the moisturizing effect and the protective effect of the skin are enhanced, and the skin is softened. It is known to improve the feel during use and after use without affecting the original functions of the detergent composition and the external preparation composition at all (Japanese Patent Application Laid-Open No. Hei. Kaihei 1-
No. 21225).

[0006] However, when this acidic polysaccharide is also made into an aqueous solution, no change in characteristics is observed at low temperature to room temperature storage, but it is unstable at high temperature storage, and
There was a problem that the viscosity decreased over time in up to two weeks, and the touch was impaired accordingly. at present,
The cause of the viscosity decrease over time is not necessarily clarified, and no method for controlling the viscosity is known.

[0007]

Accordingly, there has been a need to develop a method for suppressing a decrease in the viscosity of an aqueous solution containing an acidic polysaccharide derived from a callus culture of a plant belonging to the genus Polyanthes at a high temperature and enabling long-term stable storage. Was desired.

[0008]

Means for Solving the Problems Under such circumstances, the present inventors have conducted intensive studies in order to solve the above-mentioned problems, and as a result, the present invention contains an acidic polysaccharide derived from a callus culture of a plant belonging to the genus Polyanthes. The present inventors have found that when a specific natural acidic polysaccharide is added to an aqueous solution, a decrease in viscosity over time can be suppressed and long-term storage stability can be improved, and the present invention has been completed.

That is, the present invention is characterized by adding a natural acidic polysaccharide selected from xanthan gum, gellan gum, alginic acid, pectin and hyaluronic acid as a constituent sugar, wherein the acid polysaccharide derived from a callus culture of a plant belonging to the genus Polyanthes. It is intended to provide a method for stabilizing an aqueous solution containing a saccharide.

The acidic polysaccharide (hereinafter referred to as "acid polysaccharide") derived from a callus culture of a plant belonging to the genus Polyanthes in the present invention is disclosed in, for example, JP-A-64-10997.
In accordance with the method described in Japanese Patent Application Publication, callus derived from a plant belonging to the genus Polyanthes can be cultured and collected from the resulting culture.

Here, the method for collecting acidic polysaccharides is defined as a plant belonging to the genus Polyantes, which is tuberose ( Poli).
anthes tuberosa L .; ) As an example, a part of tuberose flowers and the like as an explant,
Callus was induced using a medium supplemented with 10 ⁻⁵ M auxin and 10 ⁻⁶ M cytokinin as plant hormones to the base medium of Linsmeier-Skoog, and further supplemented with 3% sucrose as a carbon source. The culture is performed, and the culture is further performed with shaking using a liquid medium containing the same components as the above-described callus culture medium. Thereafter, the cells are removed from the culture solution by centrifugation or filtration, and the culture solution is concentrated using a rotary evaporator or the like, and the concentrated solution is precipitated by adding a solvent such as ethanol or acetone, and the precipitate is freeze-dried. Can separate and obtain acidic polysaccharides.

The concentration of the acidic polysaccharide thus obtained in the aqueous solution is not particularly limited, and it can be used up to a concentration that can be dissolved in the aqueous medium.

In the present invention, the natural acidic polysaccharide added for stabilizing the aqueous solution containing the acidic polysaccharide is selected from xanthan gum, gellan gum, alginic acid, pectin and hyaluronic acid, and is particularly preferred. Are xanthan gum, gellan gum and pectin.
These can be used alone or in combination of two or more, and are used in an aqueous solution in an amount of 0.0001 to 10% by weight, particularly 0.1% by weight.
Preferably, 001 to 5% by weight is added. By doing so, it is possible to improve the prevention property and the stability of the viscosity of the aqueous solution over time.

In the present invention, the aqueous solution containing an acidic polysaccharide contains components other than the above-mentioned natural acidic polysaccharide, for example, inorganic salts such as sodium chloride, calcium chloride, magnesium chloride and sodium hydrogen phosphate, and lactic acid. Organic acid salts such as sodium, hydroxides such as potassium hydroxide, glycerin, propylene glycol, 1,3-butylene glycol, dipropylene glycol, polyethylene glycol, polyoxyethylene methyl glucoside, etc. Water-soluble polyhydric alcohols, sugars such as glucose, sucrose, sorbitol, and maltitol; organic acids such as citric acid and lactic acid; ethanol, amino acids, urea, proteins, surfactants, and organic substances such as p-hydroxybenzoate. May be dissolved,
Olive oil, petrolatum, liquid paraffin, higher alcohols and the like insoluble in the aqueous medium may be suspended.

[0015]

According to the stabilizing method of the present invention, a decrease in the viscosity at high temperature of an aqueous solution containing an acidic polysaccharide derived from a callus culture of a plant belonging to the genus Polyanthes is suppressed,
And stability can be improved over a long period of time.
Therefore, the aqueous solution to which the stabilization method of the present invention is applied can maintain an appropriate viscosity for a long period of time even at a high temperature, and thus, when used, an aqueous solution having a good feeling in use, an emulsion, and an external preparation in the form of a gel, A cosmetic or a cleaning agent is obtained.

[0016]

EXAMPLES The present invention will be further described below with reference to examples, but the present invention is not limited thereto.

Reference Example Production of acidic polysaccharide: Tuberose ( Polianthestuberosa L. ) was used as a plant belonging to the genus Polyanthes . The callus contains 1 × 10 ⁻⁵ M α-naphthaleneacetic acid and 1 × 10 ⁻⁶ M benzyladenine as buds of the buds of the sterilized tuberose 2 to 7 days before flowering,
Linsmai with 3% sucrose as carbon source
Induction was performed using er-Skoog's medium (agar medium). The induced calli were subcultured in the same medium. Callus that has been passaged for several generations and stabilized is 1 × as a plant hormone.
Linsmaie containing 10 ^-5 M 2,4-dichlorophenoxyacetic acid and 5% sucrose as carbon source
A medium (liquid medium) of r-Skoog was inoculated to a concentration of 5%. The culture was carried out in a dark place using a rotary shaker at a shaking number of 120 rpm at 27 ± 1 ° C. for 30 days. Thereafter, the cells were removed from the culture solution by filtration and centrifugation, and concentrated using a rotary evaporator. About 3 times the amount of ethanol is added to this concentrate,
The mixture was allowed to stand at 5 ° C. for 24 hours to obtain a precipitate. The precipitate was collected by centrifugation, washed three times with 70% ethanol,
The water was removed by freeze-drying to obtain the desired acidic polysaccharide.

Example 1 Components other than pectin in the following composition were added to a 1% by weight aqueous solution of the acidic polysaccharide obtained in the reference example, and then pectin was added. Finally, an aqueous solution containing an acidic polysaccharide having the following composition was added. Was prepared. This was stored at 50 ° C and immediately after the addition of pectin,
One month after the addition and two months after the addition, the viscosity was measured using a B-type viscometer, and the viscosity preservation ratio was determined. Table 1 shows the results when the viscosity immediately after the addition was 100%. In addition, as a comparison, the same viscosity measurement operation was carried out for the case where pectin was not added. (Blending composition) Acid polysaccharide 0.3 (% by weight) Methyl parapen 0.1 95 ° denatured alcohol 8 Sodium hydrogen phosphate 0.71 Citric acid 0.25 Pectin 0.3 86 ° Glysenline 10 Ion exchange water Balance

[0019]

[Table 1]

From the results shown in Table 1, it can be seen that the coexistence of a natural acidic polysaccharide can suppress a decrease in the viscosity of the aqueous solution containing an acidic polysaccharide.

Example 2 To the 1% by weight aqueous solution of the acidic polysaccharide obtained in Reference Example, components other than the additives in the following composition were added, and then the additives were added. Finally, the acidic polysaccharide having the following composition was added. A contained aqueous solution was prepared. For these, the viscosity preservation rate was determined immediately after the addition of the additive, 1 week after the addition, 2 weeks after the addition, and 4 weeks after the addition in the same manner as in Example 1. Table 2 shows the results. For comparison,
The same viscosity measurement operation was performed for those to which glycerin and polyethylene glycol 6000 were added and those to which no additives were added. (Blending composition) Acid polysaccharide 0.2 (% by weight) Methyl parapen 0.1 95 ° denatured alcohol 8 Additives Amount shown in Table 2 Ion exchange water Balance

[0022]

[Table 2]

From the results shown in Table 2, it can be seen that the coexistence of a natural acidic polysaccharide can suppress a decrease in the viscosity of the acidic polysaccharide-containing aqueous solution.

──────────────────────────────────────────────────続 き Continuation of front page (51) Int.Cl. ⁶ identification code FI A61K 47/36 A61K 47/36 F (58) Field surveyed (Int.Cl. ⁶ , DB name) C08B 37/00 A61K 7 / 00 A61K 7/48 A61K 47/36

Claims (1)

(57) [Claims] 1. An aqueous solution containing an acidic polysaccharide derived from a callus culture of a plant belonging to the genus Polyantes, wherein a natural acidic polysaccharide selected from xanthan gum, gellan gum, alginic acid, pectin and hyaluronic acid is added. Stabilization method.