JPH01102010A - Microbial controlling agent - Google Patents

Abstract

(57) [Summary] This bulletin contains application data before electronic filing, so abstract data is not recorded.

Description

DETAILED DESCRIPTION OF THE INVENTION (Field of Industrial Application) The present invention relates to a microbial control agent, and more particularly to a novel microbial control agent that uses Trichoderma bacteria and is safe for humans and livestock. It is.

(Prior Art) It is well known that mold and bacteria breed in buildings and everyday equipment, causing a lot of damage.

In order to prevent this, various fungicides have been developed and are commercially available, but all of these are harmful to humans and livestock, and pose a health problem.

On the other hand, it is well known that so-called antibiotics are produced because microorganisms belonging to Penicillium and Actinomyces inhibit the growth of various microorganisms, and various preparations are commercially available. The formulation also has problems in terms of safety, as side effects are unavoidable.

(Problems to be Solved by the Invention) As described above, all of the conventional antibacterial agents are harmful to humans and livestock, and pose a health safety problem.

Particularly in recent years, from the perspective of pollution prevention, antibacterial agents have been used in all industrial fields, including industrial, agricultural, horticultural, medical, and food and beverage applications. Efficacy from a safety perspective, such as toxicity and toxicity, has also become very important, and there is a strong desire to develop drugs that are harmless to humans and animals.

(Means for Solving the Problems) The present invention has been made in response to such industrial needs.

To this end, after considering various aspects such as chemistry, physics, biology, and microbiology, we decided to focus on microorganisms. The present inventors have continued research related to the fact that microorganisms belonging to Trichoderma, a type of mold, strongly produce cellulases.
When a variety of microorganisms were cultured in opposition on a vetri dish agar medium, a phenomenon was observed in which the growth of plant pathogenic molds and hyphae of edible mushrooms was significantly inhibited.

Furthermore, the same phenomenon was observed when Trichoderma viridii was inoculated after pre-growing various partner microorganisms.

These phenomena are not limited to Trichoderma viridii.
For example, as shown in Table 1, viridii showed a strong growth inhibitory effect on shiitake mushrooms, just as it did on Trichoderma longibrachiatum, but other species also showed considerable growth inhibition. It has inhibitory properties.

Table 1. Shiitake mushrooms (Lenti) by various species of the genus Trichoderma
nus edodes) growth inhibition IFO9322 Focusing on this excellent growth inhibiting property, the present inventors developed 1
- In the process of investigating the growth inhibition effect of Lycoderma bacteria on other microorganisms other than Lentinus,
We discovered that conidia that attach to the hyphae of Trichoderma fungi are closely related to this effect.

Furthermore, completely unexpectedly, even after the conidia were added to the culture medium and sterilized by heat to kill the conidia, the growth of other microorganisms inoculated with the conidia was still very large.
I also discovered that it is inhibited by the mouth. Further research was carried out, and the solution obtained by extracting single conidia with caustic soda solution was further processed, and the resulting precipitate was added to the culture medium. Even when various microorganisms were inoculated into this, most microorganisms did not survive. They also discovered that conidial extracts also have antibacterial properties.

The present invention is based on these new and useful findings,
It was finally completed as a result of further research.

One of the active ingredients of the microbial control agent according to the present invention is the conidia themselves of Trichoderma bacteria, and the other is an extract of the conidia.

Examples of Trichoderma bacteria include Trichoderma.
aKoningii, T. longibrachia
tum, T., reeSai.

In addition to T. polysporum and T. viride, all microorganisms belonging to the genus Trichoderma can be used.

Conidia of Trichoderma bacteria can be obtained either from naturally occurring bacteria or from artificially cultured bacteria. 1. In the case of artificial culture, inoculating the bacteria into an appropriate medium, Any culture method can be used, and for example, the koji making method is suitable. For this purpose, various types of grains and beans that have been heat-treated such as steaming, processed products thereof, or waste products (sugars, etc.) are required.

If necessary, water and other necessary ingredients are added to the soybean meal (soybean meal, etc.) or a mixture thereof, and this is used as a medium. After further sterilization if necessary, Trichoderma bacteria are transplanted and cultured at about 20 to 32 degrees Celsius for several days. Make koji. In this case, it is needless to say that various conventional methods for making koji can be used as appropriate; the koji obtained in this way is dried, loosened thoroughly, and passed through a sieve to obtain conidia. be.

In the present invention, the conidia themselves obtained in this manner can be used as they are, and an extract of the conidia can also be used.

A conidial extract is prepared as follows.

Conidia can be prepared at room temperature, heated, or in some cases at low temperature.

Extraction is performed using an extraction solvent under shaking, stirring, or standing conditions for a required period of time, and the obtained product (crude extract) is used as it is as an extract. When an acid or alkali is used as an extraction solvent, it is preferable to neutralize the crude extract and use it as the extract, and water, alcohol, etc. can also be used as the extract. When an extract is used, these may be removed from the crude extract by distillation, heating, reduced pressure treatment, etc., and then used as the extract.

The crude extract can be used as it is in the present invention, as well as
This may be used as it is or after an appropriate treatment, it may be subjected to centrifugation, filtration, dilution, concentration, or drying.

This microbial control agent can be used as a microbial control agent by itself as an active ingredient, and also as a preservative, fungicide, and preservative to preserve various drugs and products for a long period of time without spoiling. can also be used freely.

Therefore, the present invention provides a disinfectant for agricultural and horticultural use, a disinfectant preservative for food and beverages, a disinfectant preservative for cosmetics or pharmaceuticals, a disinfectant for sterilizing cosmetics or pharmaceuticals, a disinfectant for external or internal use, a disinfectant for soaps or detergents, and a disinfectant for construction or interior and exterior use. Wearable disinfectants, paint disinfectants, leather product disinfectants, clothing disinfectants,
It can also be used in a wide variety of other industrial and household goods for anti-bacterial, sterilizing and preserving purposes.

Hereinafter, the present invention will be further explained with reference to Examples.

Example 1 After sterilizing a mixture of 5500 g and 500 tR of tap water,
Trichoderma viridii (Trichoder＋＊a
viride) ATCC7474 and incubated at 25°C for 5
It was cultured for one day and made into koji. After drying the resulting koji in a ventilation dryer (40°C, 2 days), it was thoroughly kneaded by hand and sieved for 10 minutes using a three-stage automatic sieve grinder (100, 115, and 150 mesh). Thus, conidia were obtained.

Potato dextrose agar medium (commercially available potato dextrose agar medium 39g dissolved in 1Ω of distilled water) was used for mold, and bouillon agar medium (commercially available bouillon 30g and agar 20g dissolved in distilled water IQ) was used for bacteria. ) were prepared in advance.

To these media were added 1% each of the Trichoderma viridii conidia (air-dried) obtained above.

20 pieces of each of these were placed in a test tube and sterilized in an autoclave at 120°C for 20 minutes. After pouring these media into a Petri dish and solidifying them, each microorganism shown in Table 1 was inoculated with a platinum loop, incubated in a thermostat at 25°C for 7 days, and the growth status was observed. The results were obtained.

Table 2. Microbial growth inhibition effect by Trichoderma conidia Rice mongare fungus Pear black spot fungus Old 1610 I'1 eurotus ostraatus Oyster mushroom +++1 ±JAF'S Flama+una varietypes
+++ 10 ±Enokitake JAI”5 AIIU 9321 Ganoder+aa 1ucidu+＊
＋＋＋÷ ±Stonestone table 2 continued - No growth at all.As is clear from the above results, when one conidia is added,
It was found that the growth of all microorganisms, regardless of bacteria, filamentous fungi, and basidiomycetes, was completely inhibited, proving that the microorganism control effect of the present invention is outstanding.

Example 2 1% caustic soda solution 500+iQ was added to 10 g of conidia of Trichoderma viridii TMI 60892 prepared in the same manner as in Example 1, and the mixture was extracted at 25°C for 24 hours.
Neutralized with acid. This was then centrifuged, and 100+nQ of the resulting supernatant was added with 20+o n-butyl ether.
Q was added to obtain a colorless solidified product, which was dried with ventilation at 40°C to obtain a dried product.

0 of the above dried product was added to a potato dextrin agar medium and a bouillon agar medium prepared in the same manner as in Example 1.
．． After adding 1% concentration and sterilizing it, it was poured into a Petri dish, and in the same manner as in Example 1, various microorganisms were transplanted, cultured, and after growth, the growth status was observed, and the results shown in Table 3 were obtained.

Table 3. Growth inhibition effect of conidial extraction of Trichoderma spp. (Mucor Musedo) IFO5776 Table 3 continued - No growth at all As is clear from the above results, adding conidial extract efficiently inhibits the growth of various microorganisms. 1, proving that the microbial control effect of the present invention is outstanding.

(Effects of the Invention) The present invention uses Trichoderma bacterium conidia or its extract, which not only has an outstanding microbial control effect but also has low toxicity and is extremely safe for humans and animals. As a disinfectant for internal or external use in food and beverages, cosmetics, agriculture, horticulture, industry, and other uses, and as a preservative for preserving various products such as various drugs, industrial supplies, food and beverages, construction supplies, paints, and leather products. It can also be used extremely effectively as a fungicide.

Agent Patent Attorney 1) Parent Male

Claims (1)

[Claims] A microbial control agent consisting of conidia of Trichoderma bacteria or an extract thereof.