JPH01107958U - - Google Patents
Info
- Publication number
- JPH01107958U JPH01107958U JP397788U JP397788U JPH01107958U JP H01107958 U JPH01107958 U JP H01107958U JP 397788 U JP397788 U JP 397788U JP 397788 U JP397788 U JP 397788U JP H01107958 U JPH01107958 U JP H01107958U
- Authority
- JP
- Japan
- Prior art keywords
- fluorescence
- sample
- irradiating
- photoelectric converter
- amplification factor
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 230000005284 excitation Effects 0.000 claims description 2
- 238000004811 liquid chromatography Methods 0.000 claims description 2
- 230000003321 amplification Effects 0.000 claims 2
- 238000001514 detection method Methods 0.000 claims 2
- 230000001678 irradiating effect Effects 0.000 claims 2
- 238000003199 nucleic acid amplification method Methods 0.000 claims 2
- 230000035945 sensitivity Effects 0.000 claims 2
- 239000007788 liquid Substances 0.000 claims 1
- 238000010586 diagram Methods 0.000 description 1
Landscapes
- Spectrometry And Color Measurement (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Description
第1図は本考案の一実施例に係る液体クロマト
グラフ用蛍光分光検出器の基本構成図である。第
2図及び第3図は本実施例の効果を示す試験例の
クロマトグラムである。
10:光源、12:フローセル、14:励起側
モノクロメータ、16:光電変換器、18:蛍光
側モノクロメータ、14a,18a:回折格子、
14b,18b:ステツピングモータ、36:プ
ログラム設定器、38:制御装置。
FIG. 1 is a basic configuration diagram of a fluorescence spectroscopic detector for liquid chromatography according to an embodiment of the present invention. FIGS. 2 and 3 are chromatograms of test examples showing the effects of this example. 10: light source, 12: flow cell, 14: excitation side monochromator, 16: photoelectric converter, 18: fluorescence side monochromator, 14a, 18a: diffraction grating,
14b, 18b: stepping motor, 36: program setting device, 38: control device.
Claims (1)
起された試料から放出される蛍光のうち特定波長
の蛍光を光電変換器16に照射してクロマトグラ
ムを得る液体クロマトグラフ用蛍光分光検出器に
おいて、 感度を試料成分に応じてプログラム設定するプ
ログラム設定手段36と、 該プログラム設定手段36の出力値に応じて該
光電変換器16の検出感度または検出値増幅率可
変手段30の増幅率を切り換える制御手段38と
、 を有することを特徴とする液体クロマトグラフ
用蛍光分光検出器。[Claims for Utility Model Registration] Liquid chromatography that obtains a chromatogram by irradiating a sample in a flow cell 12 with excitation light and irradiating a photoelectric converter 16 with fluorescence of a specific wavelength among the fluorescence emitted from the excited sample. The graphing fluorescence spectrometer includes: a program setting means 36 for setting sensitivity in a program according to sample components; and means for varying the detection sensitivity or detection value amplification factor of the photoelectric converter 16 according to the output value of the program setting means 36. A fluorescence spectroscopic detector for a liquid chromatograph, comprising: a control means 38 for switching an amplification factor of 30;
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP397788U JPH01107958U (en) | 1988-01-15 | 1988-01-15 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP397788U JPH01107958U (en) | 1988-01-15 | 1988-01-15 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH01107958U true JPH01107958U (en) | 1989-07-20 |
Family
ID=31206085
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP397788U Pending JPH01107958U (en) | 1988-01-15 | 1988-01-15 |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH01107958U (en) |
-
1988
- 1988-01-15 JP JP397788U patent/JPH01107958U/ja active Pending
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Lee et al. | High-sensitivity laser-induced fluorescence detection of native proteins in capillary electrophoresis | |
| Turner | An Absolute Spectrofluorometer: A new instrument has been developed for simple determinations of absolute fluorescence spectra. | |
| Schmalzing et al. | Immunoassay for thyroxine (T4) in serum using capillary electrophoresis and micromachined devices | |
| Gluckman et al. | Laser fluorimetry for capillary column liquid chromatography: high-sensitivity detection of derivatized biological compounds | |
| Tuan et al. | Instrument for the facilitation of room temperature phosphorimetry with continuous filter paper device | |
| US6138082A (en) | Standardizing between analytical instruments | |
| Giddings | Optimized field-flow fractionation system based on dual stream splitters | |
| Amankwa et al. | Fluorescence detection in capillary electrophoresis | |
| JPH01107958U (en) | ||
| JPH01107959U (en) | ||
| Campiglia et al. | Solid-surface room-temperature phosphorescence detection for high-performance liquid chromatography | |
| JPH01107960U (en) | ||
| Polesuk et al. | Chromatographic detection | |
| Van Engelen et al. | Ultraviolet spectrometric detection for gas chromatography of polynuclear aromatic compounds with repetitive spectral scans using absorbance and concurrent fluorescence measurements | |
| Prošek et al. | Quantitative fluorodensitometric determination of ergot alkaloids | |
| Vicente et al. | A simple detection system for gas chromatography based on molecular absorption spectrometry in the gas phase | |
| Zhao et al. | A new spectrum technique based on direct detection of light intensity absorbed | |
| Rosenkranz et al. | The Plasma Emission Detector—A Suitable Detector for Speciation and Sum Parameter Analysis | |
| JPS58190731A (en) | Patsienrunge type spectrometer | |
| JPS6457157A (en) | Simple quantitative analysis method with wavelength dispersion type x-ray spectroscope | |
| RU2030732C1 (en) | Device for optical spectroscopy of materials | |
| Belenkii et al. | Use of a scanning laser fluorometric detector in high-performance thin-layer chromatography | |
| US3486304A (en) | Qualitative analysis of fluid mixtures | |
| DE60010677T2 (en) | LIGHT GENERATION, LIGHT MEASUREMENT AND DETERMINATION OF THE AMOUNT OF SUBSTANCES IN RECESSES OF MICROPLATES | |
| JPS604111Y2 (en) | infrared gas analyzer |