JPH01107959U - - Google Patents
Info
- Publication number
- JPH01107959U JPH01107959U JP397888U JP397888U JPH01107959U JP H01107959 U JPH01107959 U JP H01107959U JP 397888 U JP397888 U JP 397888U JP 397888 U JP397888 U JP 397888U JP H01107959 U JPH01107959 U JP H01107959U
- Authority
- JP
- Japan
- Prior art keywords
- fluorescence
- flow cell
- light
- excitation
- side monochromator
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 230000005284 excitation Effects 0.000 claims description 6
- 239000007788 liquid Substances 0.000 claims 1
- 238000010586 diagram Methods 0.000 description 2
- 238000001917 fluorescence detection Methods 0.000 description 1
- 238000004811 liquid chromatography Methods 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
Landscapes
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Spectrometry And Color Measurement (AREA)
Description
第１図は本考案の一実施例に係る液体クロマト
グラフ用蛍光分光検出器の基本構成図、第２図は
リテンシヨンタイムと励起波長、蛍光検出波長及
び回折格子の回転角との関係を示す図である。第
３図乃至第５図は本実施例の効果を示す試験例の
クロマトグラムである。
１０：光源、１２：フローセル、１４：励起側
モノクロメータ、１６：光電変換器、１８：蛍光
側モノクロメータ、１４ａ，１８ａ：回折格子、
１４ｂ，１８ｂ：ステツピングモータ、２６：プ
ログラム設定器、２８：制御装置、３０，３２：
ドライバ。
Fig. 1 is a basic configuration diagram of a fluorescence spectroscopic detector for liquid chromatography according to an embodiment of the present invention, and Fig. 2 shows the relationship between retention time, excitation wavelength, fluorescence detection wavelength, and rotation angle of the diffraction grating. It is a diagram. 3 to 5 are chromatograms of test examples showing the effects of this example. 10: light source, 12: flow cell, 14: excitation side monochromator, 16: photoelectric converter, 18: fluorescence side monochromator, 14a, 18a: diffraction grating,
14b, 18b: stepping motor, 26: program setting device, 28: control device, 30, 32:
driver.
Claims (1)
２と、 該光源１０からの光が入射され、特定の波長の
光を励起光として該フローセル１２へ照射する励
起側モノクロメータ１４と、 光電変換器１６と、 該フローセル１２内で励起された試料から放出
される蛍光が入射され、特定の波長の蛍光を該光
電変換器１６へ照射する蛍光側モノクロメータ１
８と、 該フローセル１２に照射する励起光の波長又は
波数、及び、該光電変換器１４に照射する蛍光の
波長又は波数を、試料成分に応じてプログラム設
定するプログラム設定手段２６と、 該プログラム設定手段２６の出力値に応じて、
該励起側モノクロメータ１４及び該蛍光側モノク
ロメータ１８の該特定波長を変化させる制御手段
２８と、 を有することを特徴とする液体クロマトグラフ
用蛍光分光検出器。[Claims for Utility Model Registration] A light source 10 and a flow cell 1 to which a sample that has passed through the column is supplied.
2, an excitation side monochromator 14 into which light from the light source 10 is incident and irradiates the flow cell 12 with light of a specific wavelength as excitation light; a photoelectric converter 16; and a sample excited within the flow cell 12. A fluorescence-side monochromator 1 receives fluorescence emitted from the fluorescence-side monochromator 1 and irradiates the photoelectric converter 16 with fluorescence of a specific wavelength.
8, a program setting means 26 for program setting the wavelength or wave number of excitation light irradiated to the flow cell 12 and the wavelength or wave number of fluorescence irradiated to the photoelectric converter 14 according to sample components; Depending on the output value of the means 26,
A fluorescence spectroscopic detector for a liquid chromatograph, comprising: control means 28 for changing the specific wavelengths of the excitation side monochromator 14 and the fluorescence side monochromator 18.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP397888U JPH01107959U (en) | 1988-01-15 | 1988-01-15 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP397888U JPH01107959U (en) | 1988-01-15 | 1988-01-15 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH01107959U true JPH01107959U (en) | 1989-07-20 |
Family
ID=31206087
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP397888U Pending JPH01107959U (en) | 1988-01-15 | 1988-01-15 |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH01107959U (en) |
-
1988
- 1988-01-15 JP JP397888U patent/JPH01107959U/ja active Pending
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