JPH0111A - Manufacturing method for refined cosmetic raw materials - Google Patents

Abstract

(57) [Summary] This bulletin contains application data before electronic filing, so abstract data is not recorded.

Description

DETAILED DESCRIPTION OF THE INVENTION [Industrial Application Field] The present invention relates to a method for producing a hydrolyzed protein or a derivative thereof which is used as a raw material in cosmetics, from which potentially insolubilized components have been removed.

[Problems to be Solved by the Prior Art and Inventions] As hydrolyzed proteins used in cosmetics, 1. Proteins of animal origin and those of plant origin are used.

Specific examples of the animal-derived products include hydrolyzates of proteins such as collagen, elastin, rechuculin, keratin, silk, milk, and chicken eggs, and specific examples of the plant-derived products include soybean 1, wheat, and corn. ,
Hydrolysates of proteins such as almonds are known (see JP-A-62-53909). Hydrolyzed proteins are commonly used in these cosmetics, but they are also incorporated into cosmetics even in solid form.

When used, it is often used as an aqueous solution. These aqueous solutions maintain the uniformity of cosmetics and increase their commercial value, so they remain transparent, cloudy, cloudy, and precipitated even after long-term storage.

I need something to calm down. Furthermore, in cosmetics containing this, 1. Particularly in cases where the cosmetic is a transparent product, it is required to remain transparent for a long period of time. Even if it is clear when filtered, it becomes cloudy or cloudy if stored for 1 to 6 months, and often forms a precipitate if stored for 6 months to 1 year or more, making it difficult to use as a raw material for cosmetics. Its commercial value has also declined, and there are restrictions on its inclusion in cosmetics.

It is known that the phenomenon of cloudiness, precipitation, etc. is more pronounced as the average molecular weight of the hydrolyzed protein is larger, and that it is more pronounced for proteins originating from vegetable proteins and keratin than for those originating from collagen, etc. There is.

This cloudiness and precipitate become transparent when filtered, but they reappear after 1 to 6 months, and even after filtration, they reappear, and there is no limit to this, and fundamentally they remain transparent for a long time. It is very difficult to produce hydrolyzed proteins or derivatives thereof.

In order to solve problems such as cloudiness and precipitation, hydrolyzed proteins are converted into derivatives, for example, hydrolyzed proteins are converted into quaternary ammonium derivatives by reaction with 3-chloro-2-hydroxypropyltrimethylammonium salt. The reality is that even in the cases where such methods are used, they are not necessarily able to solve the above-mentioned problems, and they do not always produce sufficiently satisfactory effects.

Note that as a method of hydrolyzing natural proteins, methods of hydrolyzing with acid, alkali, or proteolytic enzyme are known.

[Means for Solving the Problems] The present invention has been made in order to solve the problems such as stubborn cloudiness and precipitation that occur when hydrolyzed proteins or derivatives thereof as described above are stored as an aqueous solution. A method for producing a purified cosmetic raw material, which comprises filtering a solution of a hydrolyzed protein and/or its derivative through an ultrafiltration membrane having a molecular weight cutoff of 2000 to 200,000 to remove potentially insolubilized components. Regarding.

[Example] The hydrolyzed protein and its derivative solution in the present invention refers to a hydrolyzed protein and its derivative solution that has been conventionally used as a cosmetic raw material, and as long as it can be used as a cosmetic raw material. There are no particular limitations.

Specific examples of such solutions of hydrolyzed proteins and their derivatives include animal and vegetable protein hydrolysates and their Examples include solutions of derivatives. Examples of derivative solutions of hydrolyzed proteins other than those mentioned above include solutions in which the amino groups of hydrolyzed proteins are amidated with fatty acids, such as hydrolyzed collagen amidated with isostearic acid, such as glycidylammonium salts and hydrolyzed collagen. Examples include solutions such as ammonium salt derivatives made by reacting the hydrogen atoms of amino groups with quaternary ammonium-forming agents.
(See Japanese Patent Publication No. 82-8401).

The above-mentioned solutions of hydrolyzed proteins and their derivatives are usually produced by hydrolyzing animal or vegetable proteins using acids, alkalis, or proteolytic enzymes derived from various bacteria such as pepsin, papain, and trypsin. Furthermore, it can be obtained by quaternizing the obtained hydrolyzate.

The potential insolubilized components referred to herein refer to hydrolyzed proteins and/or derivatives thereof that have been filtered once;
When transparent cosmetics are manufactured and stored as they are or mixed into cosmetics, they become insolubilized over time and cause cloudiness and precipitation. As a result of the research conducted by the present inventors, this component mainly consists of high molecular weight components that were not sufficiently decomposed by hydrolysis; It has been found that it is a component that This insolubilization phenomenon is
The mechanism is complex and has not been fully elucidated, as it is affected by storage time, temperature, pH average molecular weight, presence of other components, etc., but in any case, a solution of hydrolyzed protein and/or its derivatives is Filtration may remove many potentially insolubilized components. Of course, this ultrafiltration also removes visible insoluble components (components visible as cloudiness, precipitates, etc.), which can also be removed by conventional filtration (e.g. filtration with a filter aid and filter paper). .

The ultrafiltration membrane used in the present invention has a molecular weight cutoff (exclusion limit molecular weight) of 2,000 to 200,000,
10,000 to about too, ooo is particularly preferable. Note that even if the membrane is not called an ultrafiltration membrane, a membrane having the above molecular weight cut-off is included in the concept of an ultrafiltration membrane in the present invention.

If the molecular weight cutoff is too small, such as less than 2,000, the passing time will be long, the yield will be low, and the ultrafiltrate will have a sticky feel. Furthermore, if the molecular weight cutoff exceeds 200,000, the removal of latent insoluble components will not be sufficient. In practice, for each hydrolyzed protein and/or its derivatives, the passage rate, yield,
It is preferable to select and use a suitable ultrafiltration membrane based on the stability of insoluble matter precipitation in the ultrafiltrate.

There are no particular limitations on the material constituting the ultrafiltration membrane, and examples include materials normally used to form ultrafiltration membranes, such as polysulfone, polyacrylonitrile, polyethylene polymers, hydrophilic polyolefins, cellulose, and cellulose acetate. Any material may be used without limitation, but if an aqueous solvent is used, a hydrophilic material is preferred.

The shape of the ultrafiltration membrane is not particularly limited, and various shapes such as a flat membrane, pleats, spirals, tubes, and hollow fibers can be used.

When removing potentially insolubilized components from a solution of a hydrolyzed protein derivative, the hydrolyzed protein solution may be passed through an ultrafiltration membrane and then converted into a derivative; You may let them.

There are no particular limitations on the conditions for ultrafiltration; normal conditions,
For example, hydrolyzed protein and/or at a concentration of 40% (wt%) or less, preferably 10 to 30%
The derivative solution was heated at a temperature of 50 to 60°C or less for 1 to 5 k.
Conditions such as g4・G are adopted.

When hydrolyzed proteins and/or their derivatives are supplied to the market to be incorporated into cosmetics, they are often in the form of dry solids (powders); If the method of the present invention is carried out in the dry solid state, it will be easier to dissolve the dry solid when it is made into a solution, and the insoluble matter will be less likely to be produced. A similar effect can be achieved by ultrafiltration.

Cosmetics in the present invention may be included in cosmetics, quasi-drugs, or pharmaceuticals as defined in the Pharmaceutical Affairs Law, and are included in the concept of cosmetics in the present invention as long as they are preparations used for cosmetics. It will be done.

Next, the method of the present invention will be explained in more detail based on Examples.

Examples 1 to 5 and Comparative Examples 1 to 2 Defatted soybean flour was hydrolyzed in water with an enzyme and then filtered using a filter aid (on diatom) and a piece of paper to obtain a transparent hydrolyzed product with an average molecular weight of 4000. Protein aqueous solution (A degree 20
%) was obtained. The resulting aqueous solution was heated at a temperature of 30°C and a pressure of 3k.
g4 was subjected to ultrafiltration using six types of ultrafiltration membranes shown in Table 1 to obtain six types of ultrafiltrate.

The resulting aqueous solution and ultrafiltrate were examined for their respective concentrations, color tone (Gardner number) immediately after production, one month later, and six months later, and transparency (visual observation). The results are shown in Table 1.

[From the results shown in Table 1 below, it can be seen that the products produced by the method of the present invention do not develop cloudiness or precipitation even after storage for 6 months, and have a light color tone, which is excellent.

Example 6 and Comparative Example 3 Hydrolyzed proteins of A-H below and their derivatives 159
The normal filtrate of each of the 6 aqueous solutions was filtered using the ultrafiltration membrane (molecular weight cut off: 50.000) used in Example 3.
Ultrafiltration was performed at a temperature of 25° C. and a pressure of 3.5 kg. The transparency of the obtained ultrafiltrate and conventional filtrate was compared immediately after production, after 1 month, and after 6 months. The results are shown in Table 2. In addition, 0.3% of Fenonip (manufactured by Balabora Reese Co., Ltd.) was added to all samples as a preservative.

A: Hydrolyzed collagen (average molecular ffi 10.00
0) B: Hydrolyzed keratin (average molecular ffi 200)
C: Hydrolyzed elastin (average molecular weight 3000) D = Hydrolyzed silk (average molecular weight Q 1000) E: Hydrolyzed wheat protein (average molecular weight 150) F: Hydrolyzed almond protein (average molecular weight 2000) G: Hydrolyzed collagen of A A quaternary ammonium derivative synthesized by reacting 3-chloro-2-hydroxypropyltrimethylammonium chloride with 3-chloro-2-hydroxypropyltrimethylammonium chloride (the amino group of hydrolyzed collagen with an average molecular weight H:A is converted into a triethanolamine salt by amidating coconut oil fatty acid) The results in Table 2 also show that the method of the present invention is effective.

[Effects of the Invention] Solutions of hydrolyzed proteins and/or derivatives thereof that are not obtained by the method of the present invention will become slightly cloudy within one week to six months in most cases, even if they are initially transparent. Transparency is lost to varying degrees from those that produce obvious precipitation. This phenomenon also occurs when it is blended into transparent cosmetics.

However, cosmetic raw materials obtained by removing potentially insolubilized components from hydrolyzed protein and/or derivative solutions by the method of the present invention remain transparent without clouding or precipitation even after a long period of time (for example, 6 months). It is. Moreover, the color tone is clearly lighter and superior than that of a sample to which the method of the present invention is not applied.

Claims (1)

[Claims] 1. A method for producing a purified cosmetic raw material, which comprises filtering a solution of a hydrolyzed protein and/or its derivative through an ultrafiltration membrane having a molecular weight cutoff of 2,000 to 200,000 to remove potentially insolubilized components.