JPH01211453A - Production of water-soluble milkprotein - Google Patents
Production of water-soluble milkproteinInfo
- Publication number
- JPH01211453A JPH01211453A JP63033980A JP3398088A JPH01211453A JP H01211453 A JPH01211453 A JP H01211453A JP 63033980 A JP63033980 A JP 63033980A JP 3398088 A JP3398088 A JP 3398088A JP H01211453 A JPH01211453 A JP H01211453A
- Authority
- JP
- Japan
- Prior art keywords
- milk
- soluble
- water
- drying
- casein
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 102000014171 Milk Proteins Human genes 0.000 title claims abstract description 19
- 108010011756 Milk Proteins Proteins 0.000 title claims abstract description 19
- 235000021239 milk protein Nutrition 0.000 title claims abstract description 19
- 238000004519 manufacturing process Methods 0.000 title claims description 6
- 235000013336 milk Nutrition 0.000 claims abstract description 21
- 239000008267 milk Substances 0.000 claims abstract description 21
- 210000004080 milk Anatomy 0.000 claims abstract description 21
- 229940108461 rennet Drugs 0.000 claims abstract description 14
- 108010058314 rennet Proteins 0.000 claims abstract description 14
- 239000005018 casein Substances 0.000 claims abstract description 13
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 claims abstract description 12
- 239000011575 calcium Substances 0.000 claims abstract description 12
- 229910052791 calcium Inorganic materials 0.000 claims abstract description 12
- BECPQYXYKAMYBN-UHFFFAOYSA-N casein, tech. Chemical compound NCCCCC(C(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(CC(C)C)N=C(O)C(CCC(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(C(C)O)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(COP(O)(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(N)CC1=CC=CC=C1 BECPQYXYKAMYBN-UHFFFAOYSA-N 0.000 claims abstract description 12
- 235000021240 caseins Nutrition 0.000 claims abstract description 12
- 238000006243 chemical reaction Methods 0.000 claims abstract description 7
- 238000001035 drying Methods 0.000 claims abstract description 7
- 238000000909 electrodialysis Methods 0.000 claims abstract description 3
- 238000000108 ultra-filtration Methods 0.000 claims abstract description 3
- 238000000034 method Methods 0.000 claims description 4
- 238000010612 desalination reaction Methods 0.000 claims description 2
- 108090000790 Enzymes Proteins 0.000 claims 1
- 102000004190 Enzymes Human genes 0.000 claims 1
- 229940088598 enzyme Drugs 0.000 claims 1
- 239000007864 aqueous solution Substances 0.000 abstract description 10
- 238000011033 desalting Methods 0.000 abstract description 7
- 229910021645 metal ion Inorganic materials 0.000 abstract description 6
- 235000020185 raw untreated milk Nutrition 0.000 abstract description 5
- 238000004108 freeze drying Methods 0.000 abstract description 2
- 238000001694 spray drying Methods 0.000 abstract description 2
- 108090000746 Chymosin Proteins 0.000 abstract 1
- 239000000843 powder Substances 0.000 description 15
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 8
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 6
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 6
- 229910052751 metal Inorganic materials 0.000 description 5
- 239000002184 metal Substances 0.000 description 5
- 239000000463 material Substances 0.000 description 4
- 235000020183 skimmed milk Nutrition 0.000 description 4
- 150000003839 salts Chemical class 0.000 description 3
- 239000011780 sodium chloride Substances 0.000 description 3
- 239000007858 starting material Substances 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- -1 Na'' Chemical class 0.000 description 2
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 description 2
- 230000015271 coagulation Effects 0.000 description 2
- 238000005345 coagulation Methods 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 229910052500 inorganic mineral Inorganic materials 0.000 description 2
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 2
- 150000002739 metals Chemical class 0.000 description 2
- 239000011707 mineral Substances 0.000 description 2
- 108010005090 rennin-like enzyme (Aspergillus ochraceus) Proteins 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 235000014036 Castanea Nutrition 0.000 description 1
- 241001070941 Castanea Species 0.000 description 1
- 229910021578 Iron(III) chloride Inorganic materials 0.000 description 1
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- 108091006629 SLC13A2 Proteins 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 150000001768 cations Chemical class 0.000 description 1
- 235000013351 cheese Nutrition 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 238000000227 grinding Methods 0.000 description 1
- 235000013402 health food Nutrition 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- RBTARNINKXHZNM-UHFFFAOYSA-K iron trichloride Chemical compound Cl[Fe](Cl)Cl RBTARNINKXHZNM-UHFFFAOYSA-K 0.000 description 1
- 239000004310 lactic acid Substances 0.000 description 1
- 235000014655 lactic acid Nutrition 0.000 description 1
- 239000008101 lactose Substances 0.000 description 1
- 239000010985 leather Substances 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 229940029985 mineral supplement Drugs 0.000 description 1
- 235000020786 mineral supplement Nutrition 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 235000018102 proteins Nutrition 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
Landscapes
- Dairy Products (AREA)
Abstract
Description
【発明の詳細な説明】
皮栗上亘剋亙分!
本発明は、ミネラル補給素材及び栄養食品の素材として
利用される、水に可溶性であってその水溶液が2価或は
3価の金属イオンにより再凝固する特性を有する水可溶
性乳タンパク質の製造方法に関する。[Detailed description of the invention] Chestnut leather is excellent! The present invention relates to a method for producing a water-soluble milk protein that is soluble in water and has the property of being re-coagulated by divalent or trivalent metal ions, and is used as a mineral supplement material and a nutritional food material. .
l米孜歪
従来、レンネット反応している乳タンパク質粉末として
は、レンネット、カゼイン、粉末チーズが知られている
が、これらは水溶性でないため、食品素材としての利用
上制約があるという問題がある。なお、牛乳をpH4,
6で等電点沈澱させて得られる酸カゼインは、そのpl
(を上げることにより水に溶解するが、上記等電点沈澱
の際のpHの調整に用いた陽イオン(主としてNa”、
K”のような1価の金属イオン)と結合していて(ソー
ダカゼイン又はボタシウムカゼイン)これら金属の含有
量が比較的多く、加うるに、カゼモノ臭が強いという利
用上の問題がある。Rennet, casein, and powdered cheese are conventionally known milk protein powders that undergo a rennet reaction, but because they are not water-soluble, there is a problem with their use as food materials. There is. In addition, milk has a pH of 4,
The acid casein obtained by isoelectric precipitation in 6 is
(Although it dissolves in water by increasing the concentration of cations (mainly Na'',
(soda casein or botanium casein) has a relatively large content of these metals, and in addition, it has a strong cold odor, which is a problem in its use.
日が ゛しようと る諜
本発明は、水に可溶性であって、Naのような金属の含
有量が極めて少くてカゼモノ臭もほとんどなく、かつ水
溶液が2価或は3価の金属イオンにより凝固する特性を
有する水可溶性乳タンパク質を製造するための方法を提
供することを課題とする。The present invention is soluble in water, has an extremely low content of metals such as Na, has almost no musty odor, and has an aqueous solution that coagulates with divalent or trivalent metal ions. An object of the present invention is to provide a method for producing a water-soluble milk protein having the following properties.
以下本発明の詳細な説明する。The present invention will be explained in detail below.
又五q盪底
本発明の特徴は、牛乳又は加工乳のpHを6.0以上に
保ちながら、その乳中のカゼインと結合しているカルシ
ウムを高度に脱塩したもの、もしくは更に濃縮したもの
を乾燥するか、もしくは凝乳酵素を添加してレンネット
反応を行った後に乾燥することにある。Another feature of the present invention is that while the pH of milk or processed milk is maintained at 6.0 or higher, calcium bound to casein in the milk is highly desalted or further concentrated. Either by drying, or by adding milk-clotting enzyme and performing a rennet reaction, followed by drying.
を”°するための
本発明においては、原料としての牛乳又は加工乳を、ア
ルカリ、例えばNaOH又はKOHによりそのpHを6
.0以上に保ちなから脱塩処理して乳中のカゼインと結
合しているカルシウムを高度に脱塩する。このカルシウ
ムの脱塩により、スターターの添加及び凝乳酵素の添加
によるカゼイン中のに−カゼインのパラ−に一カゼイン
化の後においても、凝固によるカード形成が起らなくな
る。In the present invention, milk or processed milk as a raw material is lowered to pH 6 with an alkali, such as NaOH or KOH.
.. While keeping the temperature above 0, the milk is desalinated to highly desalinate the calcium bound to casein in the milk. This desalting of calcium prevents curd formation due to coagulation even after casein is converted to para-casein by addition of starter and milk-clotting enzyme.
したがって、上記によりカルシウムを脱塩した後の原料
乳を乾燥することにより、水に可溶性な乳タンパク質粉
末が得られ、また、上記脱塩後レンネット反応を行った
ものを乾燥すると、同様に水可溶性のレンネット乳タン
パク質粉末が得られる。Therefore, by drying the raw milk after desalting calcium as described above, water-soluble milk protein powder can be obtained, and by drying the raw milk that has been subjected to the rennet reaction after desalting, it is possible to obtain water-soluble milk protein powder. A soluble rennet milk protein powder is obtained.
本発明で上記脱塩処理を行うには、電気透析法を利用す
ることが好ましく、これにより、通常、乳中のカルシウ
ムを90%脱塩し得る。すなわち、このようなカルシウ
ムの脱塩により、乳中のカゼインと結合しているカルシ
ウムが高度に脱塩されるため、得られた脱塩孔を乾燥し
た場合は勿論のこと、該脱塩孔をレンネット反応させて
も凝固カード化が起らないので、これを乾燥粉末化して
も水に可溶性となる。In order to carry out the desalting treatment in the present invention, it is preferable to use an electrodialysis method, which usually makes it possible to desalt 90% of the calcium in milk. In other words, by desalting calcium in this way, the calcium bound to casein in milk is highly desalinated. Since rennet reaction does not cause coagulation into a card, even if it is dried and powdered, it remains soluble in water.
上記脱塩孔の乾燥粉末化に際しては、該乳を濃縮しても
よく、その場合には限外濾過法を用いると効率的に行い
得る。なお、脱塩孔の乾燥は凍結乾燥又は噴霧乾燥等で
行うとよい。When drying and powdering the desalting holes, the milk may be concentrated, and in this case, ultrafiltration can be used to efficiently perform the process. Note that the desalination holes may be dried by freeze drying, spray drying, or the like.
上述のようにして得られる乳タンパク質粉末は、約25
%程度の濃度でも水によく溶解する良好な水可溶性を示
す。The milk protein powder obtained as described above has a weight of about 25
It shows good water solubility, soluble in water even at a concentration of about 1.9%.
また、乳タンパク質粉末はその水溶液に2価もしくは3
価の金属イオンを添加すると、凝固して可塑性を有する
プラスチックカードを形成する。In addition, milk protein powder is divalent or trivalent in its aqueous solution.
Addition of valent metal ions solidifies to form a plastic card.
例えば、レンネット乳タンパク質粉末の20%水溶液に
、(aclxやFe50.の2価の金属塩の水溶液を添
加するとストレッチ性のあるプラスチックカードを形成
し、FeC1,の3価の金属塩水溶液を添加するとスト
レッチ性をあまり示さないものの、良好なカードを形成
する。しかし、1価の金属塩であるNaC1やKCIの
水溶液を添加してもカードを形成しない。For example, adding an aqueous solution of a divalent metal salt of (aclx or Fe50) to a 20% aqueous solution of rennet milk protein powder forms a stretchable plastic card, and adding an aqueous solution of a trivalent metal salt of FeC1, As a result, a good card is formed although it does not exhibit much stretchability.However, even when an aqueous solution of monovalent metal salts such as NaCl or KCI is added, no card is formed.
又凱■蓋果
以上述べたように、本発明によると、水に可溶性である
とともに、その水溶液に2価もしくは3価の金属イオン
を添加すると良好なプラスチックカードを形成し、更に
成分的にはソーダカゼイン及びポタシウムカゼインと比
較し、Na及びに含有量が少ないという特性を有する乳
タンパク質粉末を得ることができる。As described above, according to the present invention, the plastic card is soluble in water, and when divalent or trivalent metal ions are added to the aqueous solution, a good plastic card is formed. It is possible to obtain a milk protein powder having a characteristic that the content of Na and Na is lower than that of soda casein and potassium casein.
したがって、本発明により得られる乳タンパク質粉末の
水溶液に、上記2価及び3価の金属イオンを栄養上有用
なミネラルを添加すると、このミネラルが結合した乳タ
ンパク質から成るカードを形成させることができる。Therefore, when nutritionally useful minerals such as the above-mentioned divalent and trivalent metal ions are added to an aqueous solution of milk protein powder obtained by the present invention, a curd consisting of milk protein bound to these minerals can be formed.
すなわち、本発明による乳タンパク質粉末は、種々の健
康食品の素材として広範囲に利用し得るので、従来の乳
タンパク質粉末にみられない用途に供することができる
。That is, the milk protein powder according to the present invention can be widely used as a material for various health foods, and therefore can be used for uses not found in conventional milk protein powders.
以下に実施例を示して本発明を具体的に説明する。なお
、実施例中の%は特記しない限り重量を示す。EXAMPLES The present invention will be specifically described below with reference to Examples. Note that % in the examples indicates weight unless otherwise specified.
実施例1
原料乳として、全固形分8.83%、脂肪0.08%、
タンパク質2.99%、乳糖4.44%及び灰分0.7
5%(カルシウム含有量124mg/100g)を含を
する脱脂乳60に、を用い、この脱脂乳を電気透析装置
(徳山曹達社製、TS−24型)により、カルシウム含
有量10.9mg/100gになるまで高度脱塩し、こ
の間脱脂乳のpHをNaOHを少量添加しながら、6.
2に保持した。Example 1 As raw milk, total solid content 8.83%, fat 0.08%,
Protein 2.99%, lactose 4.44% and ash 0.7
Using skim milk 60 containing 5% (calcium content 124 mg/100 g), this skim milk was treated with an electrodialyzer (manufactured by Tokuyama Soda Co., Ltd., TS-24 type) to have a calcium content of 10.9 mg/100 g. During this period, the pH of the skim milk was adjusted to 6.0 by adding a small amount of NaOH.
It was kept at 2.
このようにして高度脱塩した脱脂乳を65℃で30分間
低温殺菌した後、50℃まで冷却し、回分式%式%
GR61PP膜)で濃縮倍率4.5倍まで濃縮して、脱
塩濃縮乳13.3kgを得た。この脱塩swi乳12k
gを30℃に冷却した後、これに乳酸菌スターターとし
て凍結BD−スターターカルチャー0.01%を接種し
、更にレンネット (クリスチャンーハンセン社製)3
0ppmを添加して30分間レンネット反応を行った。After pasteurizing the highly desalted skim milk at 65°C for 30 minutes, it was cooled to 50°C and concentrated to a concentration ratio of 4.5 times using a batch method (% GR61PP membrane) to desalinate and concentrate. 13.3 kg of milk was obtained. This desalted swi milk 12k
After cooling the sample to 30°C, it was inoculated with 0.01% of frozen BD-starter culture as a lactic acid bacteria starter, and then rennet (Christian-Hansen) 3
A rennet reaction was carried out for 30 minutes by adding 0 ppm.
次いで、これを二等分して、一方は液体窒素で短時間凍
結して凍結乾燥を行い、他方は噴霧乾燥を行って、レン
ネット乳タンパク質粉末1.2kgを得た。また、レン
ネット添加後30分後に85℃で10分間の加熱を行っ
てレンネットを失活させた粉末を併せて得た。Next, this was divided into two parts, one part was briefly frozen in liquid nitrogen and freeze-dried, and the other part was spray-dried to obtain 1.2 kg of rennet milk protein powder. Further, 30 minutes after the addition of rennet, heating was performed at 85° C. for 10 minutes to obtain a powder in which rennet was deactivated.
これらの粉末を水で溶解したところ、いずれも25%濃
度まで溶解性は良好であった。When these powders were dissolved in water, they all had good solubility up to a concentration of 25%.
次の実施例2及び3は、本発明による乳タンパク質粉末
の応用例を示したものである。The following Examples 2 and 3 illustrate the application of the milk protein powder according to the present invention.
実施N2
実施例1で得たレンネット乳タンパク質粉末の20%水
溶液に、それぞれCaC1z、Fe50. 、NaC1
、FeC1,の各水溶液を添加したところ、NaC1で
はカードを形成しなかったが、CaC1t、Fe50.
ではストレッチ性のあるプラスチックカードを形成し、
FeCl3ではストレッチ性はあまり示さないものの、
良好なカードを形成した。Implementation N2 CaC1z, Fe50. , NaC1
When aqueous solutions of , FeCl, and NaCl were added, no cards were formed with NaCl, but with CaCl, Fe50.
Then we form a stretchy plastic card.
Although FeCl3 does not show much stretchability,
Made a good card.
実施例3Example 3
Claims (3)
、その乳中のカゼインと結合しているカルシウムを高度
に脱塩したもの、もしくは更に濃縮したものを乾燥する
か、もしくは凝乳酵素を添加してレンネツト反応を行つ
た後に乾燥することを特徴とする水可溶性乳タンパク質
の製造方法。(1) While keeping the pH of milk or processed milk at 6.0 or higher, calcium bound to casein in the milk is highly desalted, or further concentrated, and then dried, or curdled milk A method for producing a water-soluble milk protein, which comprises adding an enzyme and performing a rennet reaction, followed by drying.
1)項記載の製造方法。(2) Desalination is carried out by electrodialysis method (
The manufacturing method described in section 1).
1)項記載の製造方法。(3) Claim No. 3 in which concentration is performed by ultrafiltration method (
The manufacturing method described in section 1).
Priority Applications (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP3398088A JPH074169B2 (en) | 1988-02-18 | 1988-02-18 | Method for producing water-soluble milk protein |
| NZ227938A NZ227938A (en) | 1988-02-18 | 1989-02-10 | Process for the production of water-soluble milk protein from cow's milk |
| AU29900/89A AU618985B2 (en) | 1988-02-18 | 1989-02-13 | Water-soluble milk protein and process for producing same |
| CA 591431 CA1336240C (en) | 1988-02-18 | 1989-02-17 | Water-soluble milk protein and process for producing same |
| DK073889A DK73889A (en) | 1988-02-18 | 1989-02-17 | WATER SOLUBLE MILK PROTEIN AND PROCEDURE FOR PRODUCING THE SAME |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP3398088A JPH074169B2 (en) | 1988-02-18 | 1988-02-18 | Method for producing water-soluble milk protein |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH01211453A true JPH01211453A (en) | 1989-08-24 |
| JPH074169B2 JPH074169B2 (en) | 1995-01-25 |
Family
ID=12401634
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP3398088A Expired - Lifetime JPH074169B2 (en) | 1988-02-18 | 1988-02-18 | Method for producing water-soluble milk protein |
Country Status (5)
| Country | Link |
|---|---|
| JP (1) | JPH074169B2 (en) |
| AU (1) | AU618985B2 (en) |
| CA (1) | CA1336240C (en) |
| DK (1) | DK73889A (en) |
| NZ (1) | NZ227938A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2002143862A (en) * | 2000-11-15 | 2002-05-21 | Kurita Water Ind Ltd | Method for treating protein-containing wastewater |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2006066332A1 (en) * | 2004-12-23 | 2006-06-29 | Murray Goulburn Co-Operative Co Limited | Method of manufacture of a modified milk powder suitable as a rennet casein extender or replacer |
| US10667538B2 (en) | 2007-11-07 | 2020-06-02 | Leprino Foods Company | Non-fat dry milk production processes for cheesemaking |
-
1988
- 1988-02-18 JP JP3398088A patent/JPH074169B2/en not_active Expired - Lifetime
-
1989
- 1989-02-10 NZ NZ227938A patent/NZ227938A/en unknown
- 1989-02-13 AU AU29900/89A patent/AU618985B2/en not_active Ceased
- 1989-02-17 DK DK073889A patent/DK73889A/en not_active Application Discontinuation
- 1989-02-17 CA CA 591431 patent/CA1336240C/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2002143862A (en) * | 2000-11-15 | 2002-05-21 | Kurita Water Ind Ltd | Method for treating protein-containing wastewater |
Also Published As
| Publication number | Publication date |
|---|---|
| CA1336240C (en) | 1995-07-11 |
| DK73889A (en) | 1989-08-19 |
| AU2990089A (en) | 1989-08-24 |
| AU618985B2 (en) | 1992-01-16 |
| NZ227938A (en) | 1990-06-26 |
| DK73889D0 (en) | 1989-02-17 |
| JPH074169B2 (en) | 1995-01-25 |
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