JPH01224667A - Appliance for detecting ketone body - Google Patents
Appliance for detecting ketone bodyInfo
- Publication number
- JPH01224667A JPH01224667A JP5229788A JP5229788A JPH01224667A JP H01224667 A JPH01224667 A JP H01224667A JP 5229788 A JP5229788 A JP 5229788A JP 5229788 A JP5229788 A JP 5229788A JP H01224667 A JPH01224667 A JP H01224667A
- Authority
- JP
- Japan
- Prior art keywords
- nitroprusside
- appliance
- alkaline buffer
- silane
- deriv
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 150000002576 ketones Chemical class 0.000 title claims abstract description 33
- YEESUBCSWGVPCE-UHFFFAOYSA-N azanylidyneoxidanium iron(2+) pentacyanide Chemical class [Fe++].[C-]#N.[C-]#N.[C-]#N.[C-]#N.[C-]#N.N#[O+] YEESUBCSWGVPCE-UHFFFAOYSA-N 0.000 claims abstract description 18
- BLRPTPMANUNPDV-UHFFFAOYSA-N Silane Chemical compound [SiH4] BLRPTPMANUNPDV-UHFFFAOYSA-N 0.000 claims abstract 5
- 150000004756 silanes Chemical class 0.000 claims description 21
- 239000000126 substance Substances 0.000 claims description 7
- 125000001424 substituent group Chemical group 0.000 claims description 4
- 125000004432 carbon atom Chemical group C* 0.000 claims description 3
- 125000003342 alkenyl group Chemical group 0.000 claims description 2
- 125000000217 alkyl group Chemical group 0.000 claims description 2
- 229910052739 hydrogen Inorganic materials 0.000 claims description 2
- 239000001257 hydrogen Substances 0.000 claims description 2
- 125000004435 hydrogen atom Chemical class [H]* 0.000 claims description 2
- 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 claims description 2
- 125000001997 phenyl group Chemical group [H]C1=C([H])C([H])=C(*)C([H])=C1[H] 0.000 claims description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims 1
- 229910052799 carbon Inorganic materials 0.000 claims 1
- XEYBHCRIKKKOSS-UHFFFAOYSA-N disodium;azanylidyneoxidanium;iron(2+);pentacyanide Chemical compound [Na+].[Na+].[Fe+2].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-].[O+]#N XEYBHCRIKKKOSS-UHFFFAOYSA-N 0.000 abstract description 9
- 229940083618 sodium nitroprusside Drugs 0.000 abstract description 9
- 210000002700 urine Anatomy 0.000 abstract description 9
- 210000001124 body fluid Anatomy 0.000 abstract description 6
- 150000001413 amino acids Chemical class 0.000 abstract description 5
- 239000003153 chemical reaction reagent Substances 0.000 abstract description 5
- 239000003795 chemical substances by application Substances 0.000 abstract description 5
- 238000001514 detection method Methods 0.000 abstract description 5
- 239000001488 sodium phosphate Substances 0.000 abstract description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract description 5
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 abstract description 4
- 238000000354 decomposition reaction Methods 0.000 abstract description 4
- 239000007788 liquid Substances 0.000 abstract description 4
- BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical compound [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 abstract description 3
- 229910000397 disodium phosphate Inorganic materials 0.000 abstract description 3
- 235000019800 disodium phosphate Nutrition 0.000 abstract description 3
- 239000004471 Glycine Substances 0.000 abstract description 2
- OLLFKUHHDPMQFR-UHFFFAOYSA-N dihydroxy(diphenyl)silane Chemical compound C=1C=CC=CC=1[Si](O)(O)C1=CC=CC=C1 OLLFKUHHDPMQFR-UHFFFAOYSA-N 0.000 abstract description 2
- 229910000077 silane Inorganic materials 0.000 abstract 4
- 238000007689 inspection Methods 0.000 abstract 2
- 238000004321 preservation Methods 0.000 abstract 1
- 238000012360 testing method Methods 0.000 description 8
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 6
- ZMXDDKWLCZADIW-UHFFFAOYSA-N N,N-Dimethylformamide Chemical compound CN(C)C=O ZMXDDKWLCZADIW-UHFFFAOYSA-N 0.000 description 6
- 238000002360 preparation method Methods 0.000 description 6
- 239000000243 solution Substances 0.000 description 6
- 239000010839 body fluid Substances 0.000 description 5
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 4
- 235000001014 amino acid Nutrition 0.000 description 4
- 238000006243 chemical reaction Methods 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- 239000008213 purified water Substances 0.000 description 3
- WDJHALXBUFZDSR-UHFFFAOYSA-N Acetoacetic acid Natural products CC(=O)CC(O)=O WDJHALXBUFZDSR-UHFFFAOYSA-N 0.000 description 2
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 2
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 2
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 description 2
- 239000002250 absorbent Substances 0.000 description 2
- 230000002745 absorbent Effects 0.000 description 2
- 230000002378 acidificating effect Effects 0.000 description 2
- 238000007796 conventional method Methods 0.000 description 2
- WOWBFOBYOAGEEA-UHFFFAOYSA-N diafenthiuron Chemical compound CC(C)C1=C(NC(=S)NC(C)(C)C)C(C(C)C)=CC(OC=2C=CC=CC=2)=C1 WOWBFOBYOAGEEA-UHFFFAOYSA-N 0.000 description 2
- OSXYHAQZDCICNX-UHFFFAOYSA-N dichloro(diphenyl)silane Chemical compound C=1C=CC=CC=1[Si](Cl)(Cl)C1=CC=CC=C1 OSXYHAQZDCICNX-UHFFFAOYSA-N 0.000 description 2
- 238000001035 drying Methods 0.000 description 2
- -1 jetoxydiphenylsilane Chemical compound 0.000 description 2
- 230000007774 longterm Effects 0.000 description 2
- 239000002985 plastic film Substances 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 239000012266 salt solution Substances 0.000 description 2
- UEUXEKPTXMALOB-UHFFFAOYSA-J tetrasodium;2-[2-[bis(carboxylatomethyl)amino]ethyl-(carboxylatomethyl)amino]acetate Chemical class [Na+].[Na+].[Na+].[Na+].[O-]C(=O)CN(CC([O-])=O)CCN(CC([O-])=O)CC([O-])=O UEUXEKPTXMALOB-UHFFFAOYSA-J 0.000 description 2
- RYFMWSXOAZQYPI-UHFFFAOYSA-K trisodium phosphate Chemical compound [Na+].[Na+].[Na+].[O-]P([O-])([O-])=O RYFMWSXOAZQYPI-UHFFFAOYSA-K 0.000 description 2
- 229910000406 trisodium phosphate Inorganic materials 0.000 description 2
- 235000019801 trisodium phosphate Nutrition 0.000 description 2
- SLXKOJJOQWFEFD-UHFFFAOYSA-N 6-aminohexanoic acid Chemical compound NCCCCCC(O)=O SLXKOJJOQWFEFD-UHFFFAOYSA-N 0.000 description 1
- 208000001380 Diabetic Ketoacidosis Diseases 0.000 description 1
- QNAYBMKLOCPYGJ-REOHCLBHSA-N L-alanine Chemical compound C[C@H](N)C(O)=O QNAYBMKLOCPYGJ-REOHCLBHSA-N 0.000 description 1
- 239000004349 Polyvinylpyrrolidone-vinyl acetate copolymer Substances 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 235000004279 alanine Nutrition 0.000 description 1
- 229960002684 aminocaproic acid Drugs 0.000 description 1
- 238000010420 art technique Methods 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 229910021538 borax Inorganic materials 0.000 description 1
- 230000023852 carbohydrate metabolic process Effects 0.000 description 1
- 235000021256 carbohydrate metabolism Nutrition 0.000 description 1
- 239000000969 carrier Substances 0.000 description 1
- 229920001577 copolymer Polymers 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- OSMIWEAIYFILPL-UHFFFAOYSA-N dibutoxy(diphenyl)silane Chemical compound C=1C=CC=CC=1[Si](OCCCC)(OCCCC)C1=CC=CC=C1 OSMIWEAIYFILPL-UHFFFAOYSA-N 0.000 description 1
- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- JJQZDUKDJDQPMQ-UHFFFAOYSA-N dimethoxy(dimethyl)silane Chemical compound CO[Si](C)(C)OC JJQZDUKDJDQPMQ-UHFFFAOYSA-N 0.000 description 1
- XCLIHDJZGPCUBT-UHFFFAOYSA-N dimethylsilanediol Chemical compound C[Si](C)(O)O XCLIHDJZGPCUBT-UHFFFAOYSA-N 0.000 description 1
- UQGFMSUEHSUPRD-UHFFFAOYSA-N disodium;3,7-dioxido-2,4,6,8,9-pentaoxa-1,3,5,7-tetraborabicyclo[3.3.1]nonane Chemical compound [Na+].[Na+].O1B([O-])OB2OB([O-])OB1O2 UQGFMSUEHSUPRD-UHFFFAOYSA-N 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000000194 fatty acid Substances 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
- 150000004665 fatty acids Chemical class 0.000 description 1
- 239000003365 glass fiber Substances 0.000 description 1
- 238000005470 impregnation Methods 0.000 description 1
- 239000002075 main ingredient Substances 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 229920000728 polyester Polymers 0.000 description 1
- 235000019448 polyvinylpyrrolidone-vinyl acetate copolymer Nutrition 0.000 description 1
- 229910000029 sodium carbonate Inorganic materials 0.000 description 1
- 235000017550 sodium carbonate Nutrition 0.000 description 1
- 159000000000 sodium salts Chemical class 0.000 description 1
- 239000004328 sodium tetraborate Substances 0.000 description 1
- 235000010339 sodium tetraborate Nutrition 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
Abstract
Description
【発明の詳細な説明】
（産業上の利用分野）
本発明は、体液、殊に尿中のケトン体を検出するための
検出用器具に関する。DETAILED DESCRIPTION OF THE INVENTION (Field of Industrial Application) The present invention relates to a detection instrument for detecting ketone bodies in body fluids, particularly urine.
（従来の技術）
ケトン体は、アセト酢酸、β−オキシ酢酸、アセトン等
の総称で、生体内では主に脂肪酸から生成される。(Prior Art) Ketone bodies are a general term for acetoacetic acid, β-oxyacetic acid, acetone, etc., and are mainly produced from fatty acids in living organisms.
健常者の場合でも微量のケトン体がアセトンとして１日
０．０１〜０．０２９程度尿中に排出されるが、糖質代
謝が不完全な場合には血中のケトン体が増加すると共に
、尿中にも多量に排出されるようになる。Even in healthy people, trace amounts of ketone bodies are excreted in the urine as acetone, about 0.01 to 0.029 per day, but when carbohydrate metabolism is incomplete, the amount of ketone bodies in the blood increases. Large amounts are also excreted in the urine.
臨床的には種々の要因でケトン体が増えるが、とりわけ
糖尿病性ケトアシド−シスの診断に尿中のケトン体の検
査は有用である。Clinically, ketone bodies increase due to various factors, but testing for ketone bodies in urine is particularly useful for diagnosing diabetic ketoacidosis.
体液中のケトン体の検出には、ケトン体とニトロプルシ
ドナトリウムの反応が知られている。この反応はアルカ
リ性条件下で進行するが、主剤のニトロプルシドナトリ
ウムがアルカリ性条件下では急速に分解してしまうとい
う欠点があるため、この分解を防止すべく、ケトン体の
検出用器具に種々の改良が加えられてきた。A reaction between ketone bodies and sodium nitroprusside is known for detecting ketone bodies in body fluids. This reaction proceeds under alkaline conditions, but the drawback is that the main ingredient, sodium nitroprusside, decomposes rapidly under alkaline conditions.In order to prevent this decomposition, various improvements have been made to devices for detecting ketone bodies. has been added.
そのような従来の技術の例には、ニトロプルシドナトリ
ウムを酸性ｐＨの有機フィルム形成物質でアルカリ性条
件から保護する方法や、ニトロプルシドナトリウムと共
にアルカリ性緩衝剤としてＥ Ｄ ’ｌ’ Ａナトリウ
ム塩を用い、更に保護剤としてジメチルホルムアミドを
加えろ方法などがある。Examples of such prior art techniques include protecting sodium nitroprusside from alkaline conditions with organic film-forming substances at acidic pH, and using E D 'l' A sodium salt as an alkaline buffer along with sodium nitroprusside and further protecting it. There is a method of adding dimethylformamide as an agent.
（発明が解決しようとする課題）
しかし、このような従来のケトン体の検出法には種々の
問題かある。(Problems to be Solved by the Invention) However, such conventional methods for detecting ketone bodies have various problems.
まず、有機フィルム形成物質でニトロプルシドナトリウ
ムを保護する方法では、有機フィルム形成物質としてポ
リビニルピロリドン−酢酸ビニル共重合体や、メチルエ
チルエーテル−無水マレイン酸共重合体などを用いるが
、このような有機フィルム形成物質は多量に使用しない
と安定性が悪く、しかしながら、多量に使用することに
上りケトン体との反応速度が低下するという欠点を有す
る。First, in the method of protecting sodium nitroprusside with an organic film-forming substance, polyvinylpyrrolidone-vinyl acetate copolymer, methyl ethyl ether-maleic anhydride copolymer, etc. are used as the organic film-forming substance. The forming substance has poor stability unless used in a large amount, however, it has the disadvantage that the rate of reaction with the ketone body decreases when used in a large amount.
また、ＥＤＴＡナトリウム塩とジメチルホルムアミドを
用いる方法では、ニトロプルシドナトリウムの分解を完
全に止めることができず、長期間の保存で問題がある。Furthermore, in the method using EDTA sodium salt and dimethylformamide, the decomposition of sodium nitroprusside cannot be completely stopped, and there is a problem in long-term storage.
本発明の目的は、このような従来のケトン体の検出法を
改良し、体液、殊に尿中のケトンを容易に検出でき、臨
床検査の分野で用いることができるようなケトン体の検
出用器具を提供することにある。The purpose of the present invention is to improve such a conventional method for detecting ketone bodies, to easily detect ketones in body fluids, especially urine, and to provide a method for detecting ketone bodies that can be used in the field of clinical testing. The goal is to provide equipment.
（課題を解決するための手段）
本発明者らは、上述のような課題を解決し、その目的を
達成するために鋭意研究を進めた結果、一般式：
［式中、Ｒ１はフェニル基又はメチル基を表わし、Ｒ２
は水素、置換基を有することもある炭素数１〜１８個の
直鎖状または分岐状アルキル基又は置換基を有すること
もある炭素数２〜１８の直鎖状または分岐状アルケニル
基を表す。］
で示されるシラン誘導体を用いることにより、上述のよ
うな課題が解決されることを見い出し、更に研究を進め
た結果、ニトロプルシド塩、アルカリ性緩衝剤および該
シラン誘導体を含有することを特徴とするケトン体の検
出用器具を完成するに至った。(Means for Solving the Problems) The present inventors have solved the above-mentioned problems and, as a result of intensive research to achieve the objective, have found that the general formula: [wherein R1 is a phenyl group or Represents a methyl group, R2
represents hydrogen, a linear or branched alkyl group having 1 to 18 carbon atoms which may have a substituent, or a linear or branched alkenyl group having 2 to 18 carbon atoms which may have a substituent. ] It was discovered that the above-mentioned problems could be solved by using the silane derivative shown in the following, and as a result of further research, a ketone characterized by containing a nitroprusside salt, an alkaline buffer, and the silane derivative was found. We have completed a body detection device.
即ち、本発明はニトロプルシド塩、アルカリ性緩衝剤お
よびシラン誘導体（Ｉ）を吸液性担体に含浸させること
により、ケトン体との反応速度を低下させることなく、
ニトロプルシド塩の分解を防止することができるケトン
体の検出用器具を提供するものである。That is, the present invention impregnates a liquid-absorbing carrier with a nitroprusside salt, an alkaline buffer, and a silane derivative (I), thereby reducing the rate of reaction with a ketone body.
The present invention provides a device for detecting ketone bodies that can prevent the decomposition of nitroprusside salts.
本発明のケトン体の検出用器具は、ニトロプルシド塩層
とアルカリ性緩衝剤層の間にシラン誘導体（１）層を存
在させることにより、保存中のニトロプルシド塩の分解
を防止することができる。これはシラン誘導体（１）層
が酸性であり、かつ耐水性の層を形成するため、ニトロ
プルシド塩が直接アルカリ層に触れるのを防ぎ、かつ吸
湿などによりニトロプルシド塩がアルカリ層に溶出する
ことも防止するため、長期間の保存安定性に極めて優れ
ている。The ketone body detection device of the present invention can prevent the nitroprusside salt from decomposing during storage by providing the silane derivative (1) layer between the nitroprusside salt layer and the alkaline buffer layer. This is because the silane derivative (1) layer is acidic and forms a water-resistant layer, which prevents the nitroprusside salt from coming into direct contact with the alkaline layer, and also prevents the nitroprusside salt from eluting into the alkaline layer due to moisture absorption, etc. Therefore, it has excellent long-term storage stability.
本発明のケトン体の検出用器具は、臨床検査で用いる場
合、使用上の簡便性や試薬の安定性を考慮し、スティッ
クの形態とするのが好ましい。このようなスティックを
作製するには、まずアルカリ性緩衝剤を吸液性担体に含
浸し、乾燥させた後、シラン誘導体（１）を含む溶液を
担体に含浸し、乾燥させ、その後、ニトロプルシド塩を
含む溶液を担体に含浸し、乾燥させて試験紙を作る。こ
のような吸液性担体の例としては、濾紙、ガラス繊維紙
、ポリエステルフリースなどが挙げられる。次に、これ
らの試薬を含浸した試験紙をプラスチックシートなどの
支持体に貼合し、スティックの形態とする。When the device for detecting ketone bodies of the present invention is used in a clinical test, it is preferably in the form of a stick in consideration of ease of use and stability of the reagent. To make such a stick, first, a liquid-absorbing carrier is impregnated with an alkaline buffer and dried, then a solution containing the silane derivative (1) is impregnated into the carrier and dried, and then a nitroprusside salt is impregnated into the carrier. A test strip is made by impregnating a carrier with the solution and drying it. Examples of such liquid-absorbing carriers include filter paper, glass fiber paper, polyester fleece, and the like. Next, the test paper impregnated with these reagents is pasted onto a support such as a plastic sheet to form a stick.
本発明のケトン体の検出用器具に用いるニトロプルシド
塩の例としては、ニトロプルシドナトリウムがある。An example of the nitroprusside salt used in the device for detecting ketone bodies of the present invention is sodium nitroprusside.
アルカリ性緩衝剤は本発明の目的にかなうものであれば
、特に限定されないが、通常水溶性アミノ酸及びアルカ
リ剤から組合せて選ぶことができる。水溶性アミノ酸の
例としては、グリシン、アラニン、ε−アミノカプロン
酸などが挙げられ、アルカリ剤の例としてはリン酸二ナ
トリウム、リン酸三ナトリウム、炭酸ナトリウム、四ホ
ウ酸ナトリウム、ＥＤＴＡナトリウム塩などが挙げられ
る。The alkaline buffer is not particularly limited as long as it meets the purpose of the present invention, but it can usually be selected in combination from water-soluble amino acids and alkaline agents. Examples of water-soluble amino acids include glycine, alanine, and ε-aminocaproic acid. Examples of alkaline agents include disodium phosphate, trisodium phosphate, sodium carbonate, sodium tetraborate, and EDTA sodium salt. Can be mentioned.
シラン誘導体（Ｉ）の具体例としては、ジフェニルシラ
ンジオール、ジェトキシジフェニルシラン、ジメチルシ
ランジオール、ジメトキシジメチルシラン、ジブトキシ
ジフェニルシラン、ジオレイルオキシジフェニルシラン
、２−エチルアミノエトキシジフェニルシランなどが挙
げられる。Specific examples of the silane derivative (I) include diphenylsilanediol, jetoxydiphenylsilane, dimethylsilanediol, dimethoxydimethylsilane, dibutoxydiphenylsilane, dioleyloxydiphenylsilane, 2-ethylaminoethoxydiphenylsilane, and the like. .
本発明のケトン体の検出用器具を作製するに際して、試
験紙に含浸させる試薬の濃度は一概に定めることができ
ないが、ニトロプルシド塩は通常０．１−１０９／ｄ１
２．好ましくは０５５〜３９／ｄＱの濃度で吸液性担体
に含浸させるのがよい。また、アルカリ性緩衝剤を構成
する水溶性アミノ酸は通常３〜４０ ｇ／ｄ（１，好ま
しく ハ５〜３０９／ｄ（１（Ｄ濃度で、アルカリ剤は
通常３〜３０ｇ／ｄＱ、好ましくはＩＯ〜２５ｇ／ｄ＋
２の濃度でそれぞれ混合してアルカリ性緩衝剤とし、吸
液性担体に含浸させる。When producing the device for detecting ketone bodies of the present invention, the concentration of the reagent to be impregnated into the test paper cannot be determined unconditionally, but the concentration of nitroprusside salt is usually 0.1-109/d1.
2. It is preferable to impregnate the liquid absorbent carrier at a concentration of 055 to 39/dQ. In addition, the water-soluble amino acids constituting the alkaline buffer are usually 3 to 40 g/d (1, preferably 5 to 309/d (1 (D concentration), and the alkaline agent is usually 3 to 30 g/dQ, preferably IO to 25g/d+
The two concentrations are mixed to form an alkaline buffer, and the liquid absorbent carrier is impregnated with the alkaline buffer.
シラン誘導体（Ｉ）は通常０．１〜１０ｙ／ｄｃ、好ま
しくは０．５〜３ ｇｄＱの濃度で吸液性担体に含浸さ
せるのがよい。The silane derivative (I) is preferably impregnated into the liquid-absorbing carrier at a concentration of usually 0.1 to 10 y/dc, preferably 0.5 to 3 gdQ.
このようにして作製したケトン体の検出用器具は、これ
を被検体としての体液、特に尿中に浸し、その呈色を既
知のケトン体濃度に対する呈色と比較することにより、
ケトン体を検出することができる。The device for detecting ketone bodies produced in this way is immersed in a body fluid, especially urine, as a test subject, and its coloration is compared with that of a known concentration of ketone bodies.
Ketone bodies can be detected.
（実施例）
次に本発明の実施例を示すが、これらは本発明を何等限
定するものではない。(Example) Next, Examples of the present invention will be shown, but these are not intended to limit the present invention in any way.
実施例１
本発明のケトン体の検出用器具の例であるスティックは
、次のようにして作製した。Example 1 A stick, which is an example of the device for detecting ketone bodies of the present invention, was produced as follows.
１、試薬の調製
（１）アルカリ性緩衝剤の調製
アミノ酸２５ｇ、リン酸二ナトリウム１２９およびリン
酸三ナトリウム５ｇを精製水１００ｍｇで溶解する。1. Preparation of reagents (1) Preparation of alkaline buffer Dissolve 25 g of amino acids, 129 disodium phosphate, and 5 g of trisodium phosphate in 100 mg of purified water.
（２）シラン誘導体（１）溶液の調製
ジクロルジフェニルシラン１２ｇに精製水２０ｔｔｔＱ
を加えて混和した後、固体成分をとり少量の精製水で洗
浄して風乾し、白色物質を得る。この白色物質をクロロ
ホルム２ｇに溶解する。(2) Preparation of silane derivative (1) solution Add 12 g of dichlorodiphenylsilane to 20 tttQ of purified water.
After adding and mixing, the solid component is taken out, washed with a small amount of purified water, and air-dried to obtain a white substance. This white substance is dissolved in 2 g of chloroform.
（３）ニトロプルシド塩溶液の調製
ニトロプルシドナトリウム１ 、５９、ジメチルスルホ
キシド３０ｘ（ｌおよびエタノール７０ｘＱを混合して
溶解する。(3) Preparation of nitroprusside salt solution Mix and dissolve sodium nitroprusside 1,59, dimethyl sulfoxide 30x (l) and ethanol 70xQ.
２、スティックの作製
（１）アルカリ性緩衝剤を濾紙（東洋濾紙５１４Ａ）に
含浸させ、３７℃で一昼夜乾燥後、さらに８０℃で２時
間乾燥させ、次に（２）シラン誘導体（１）溶液を含浸
させ、８０℃で３０分間乾燥させる。更に（３）ニトロ
プルシド塩溶液を含浸させ、８０℃で３０分間乾燥後、
その小片をプラスチックシートに貼合してスティックを
作製した。2. Preparation of sticks (1) Filter paper (Toyo Roshi 514A) was impregnated with an alkaline buffer and dried at 37°C for a day and night, then further dried at 80°C for 2 hours, and then (2) silane derivative (1) solution was added. Impregnate and dry at 80°C for 30 minutes. Furthermore, (3) impregnation with nitroprusside salt solution and drying at 80°C for 30 minutes,
A stick was made by pasting the small pieces onto a plastic sheet.
実施例２
（２）シラン誘導体（１）溶液を以下のように調製する
以外は実施例１の手順に従って、同様にスティックを作
製した。Example 2 (2) A stick was produced in the same manner as in Example 1, except that the silane derivative (1) solution was prepared as follows.
（２）シラン誘導体（Ｉ）溶液の調製
ジクロルジフェニルシラン１２ｇにエタノール５Ｊ１１
２を滴下して加え、十分に撹拌する。更にエタノールを
加えて全ｆｆ１ｌ（２となるように溶解する。(2) Preparation of silane derivative (I) solution 12 g of dichlorodiphenylsilane and 5 J11 of ethanol
Add 2 dropwise and stir thoroughly. Furthermore, add ethanol and dissolve so that the total volume is 1 liter (2 liters).
実施例３
実施例１で作製したスティックを、次のような既知の濃
度でケトン体としてアセト酢酸を含む尿に浸すと、３０
秒以内に呈色が観察され、ケトン体の検出ができること
がわかった。Example 3 When the stick prepared in Example 1 is immersed in urine containing acetoacetic acid as a ketone body at the following known concentration, 30
Color development was observed within seconds, indicating that ketone bodies could be detected.
また、実施例２で作製したスティックについても同様に
試験したところ、同様の結果が得られた。Furthermore, when the stick produced in Example 2 was similarly tested, similar results were obtained.
（発明の効果）
本発明のケトン体の検出用器具は、従来のタイプのもの
に比べ保存安定性が優れている上、スティックの形態を
とることにより迅速かつ簡便に体液、殊に尿中のケトン
体の検出ができることから、臨床検査に大きく寄与でき
るものである。(Effects of the Invention) The device for detecting ketone bodies of the present invention has superior storage stability compared to conventional types, and because it takes the form of a stick, it can quickly and easily detect body fluids, especially urine. Since it can detect ketone bodies, it can greatly contribute to clinical testing.
Claims (1)
： ▲数式、化学式、表等があります▼（ I ） ［式中、Ｒ＿１はフェニル基又はメチル基を表わし、Ｒ
＿２は水素、置換基を有することもある炭素数１〜１８
個の直鎖状または分岐状アルキル基又は置換基を有する
こともある炭素数２〜１８の直鎖状または分岐状アルケ
ニル基を表す。］ で示されるシラン誘導体を吸液性担体に含浸させて成る
ケトン体の検出用器具。 ２、シラン誘導体を含浸させた吸液性担体を支持体に結
合したスティック状である特許請求の範囲第１項記載の
ケトン体の検出用器具。[Claims] 1. Nitroprusside salt, alkaline buffer, and general formula: ▲ Numerical formulas, chemical formulas, tables, etc. ▼ (I) [In the formula, R_1 represents a phenyl group or a methyl group, and R
_2 is hydrogen, carbon number 1-18 that may have a substituent
represents a straight-chain or branched alkyl group or a straight-chain or branched alkenyl group having 2 to 18 carbon atoms which may have a substituent. ] A device for detecting ketone bodies, comprising a liquid-absorbing carrier impregnated with a silane derivative represented by the following. 2. The device for detecting ketone bodies according to claim 1, which is in the form of a stick in which a liquid-absorbing carrier impregnated with a silane derivative is bonded to a support.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP5229788A JPH01224667A (en) | 1988-03-03 | 1988-03-03 | Appliance for detecting ketone body |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP5229788A JPH01224667A (en) | 1988-03-03 | 1988-03-03 | Appliance for detecting ketone body |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH01224667A true JPH01224667A (en) | 1989-09-07 |
Family
ID=12910862
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP5229788A Pending JPH01224667A (en) | 1988-03-03 | 1988-03-03 | Appliance for detecting ketone body |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH01224667A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6160950A (en) * | 1996-07-18 | 2000-12-12 | Matsushita Electric Industrial Co., Ltd. | Method and apparatus for automatically generating a digest of a program |
| CN101833006A (en) * | 2009-03-13 | 2010-09-15 | 云南农业大学 | Method for preparing test paper for rapidly determining dairy cows subclinical ketosis |
| JP2018009795A (en) * | 2016-07-11 | 2018-01-18 | 震 古賀 | Specimen for detecting components in body fluids |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS547993A (en) * | 1977-06-20 | 1979-01-20 | Terumo Corp | Test piece for detecting keton member |
| JPS567058A (en) * | 1979-06-22 | 1981-01-24 | Miles Lab | Tester for detecting presence of constituent in liquid sample and method of making said tester |
-
1988
- 1988-03-03 JP JP5229788A patent/JPH01224667A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS547993A (en) * | 1977-06-20 | 1979-01-20 | Terumo Corp | Test piece for detecting keton member |
| JPS567058A (en) * | 1979-06-22 | 1981-01-24 | Miles Lab | Tester for detecting presence of constituent in liquid sample and method of making said tester |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6160950A (en) * | 1996-07-18 | 2000-12-12 | Matsushita Electric Industrial Co., Ltd. | Method and apparatus for automatically generating a digest of a program |
| CN101833006A (en) * | 2009-03-13 | 2010-09-15 | 云南农业大学 | Method for preparing test paper for rapidly determining dairy cows subclinical ketosis |
| CN101833006B (en) | 2009-03-13 | 2013-01-30 | 云南农业大学 | A test paper preparation method for rapid detection of subclinical ketosis in cows |
| JP2018009795A (en) * | 2016-07-11 | 2018-01-18 | 震 古賀 | Specimen for detecting components in body fluids |
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