JPH01250065A - Detection and analysis of organic nitro compound - Google Patents

Abstract

(57) [Summary] This bulletin contains application data before electronic filing, so abstract data is not recorded.

Description

[Detailed description of the invention] Industrial application field The present invention is for analyzing and analyzing organic nitro compounds that exist in extremely small amounts.
The present invention relates to a device for detecting. In particular, if collected and concentrated from the atmosphere, it can be used to detect and detect explosives, bombs, guns, pistols, etc. that use organic nitro compounds as explosives, so they cannot be illegally imported or brought onto airplanes. It can be widely used in industrial fields such as improving social safety and suppressing and preventing terrorist acts.

Conventional Technology Devices for detecting and detecting homemade time bombs, dynamite, pistols, etc. planted in travel cargo, baggage, mail, etc. are capable of detecting explosives generated from the explosives themselves or from pistols that have been fired. Mass spectrometers, gas chromatography devices, X-ray devices as metal detectors, and the like are used to analyze organic nitro compounds present.

Problems to be Solved by the Invention The present invention solves the following problems:
The purpose is to solve issues such as size, vertical surface, and price. That is, even if the existing analyzers described above are improved for this application, there is a limit to the time required for measurements, which is no more than about 10 to 20 minutes. Furthermore, the device is large and heavy, making it difficult to move, and is relatively expensive. The present invention aims to alleviate and solve these problems.

Means to solve the problem apply immunochemical analysis methods. That is, an organic nitro compound to be detected is chemically bonded to a protein, and an animal is immunized with this to obtain an antibody against the organic nitro compound. Next, trace amounts of organic nitro compounds present in the air are collected and concentrated, and the antibodies are subjected to an antigen-antibody reaction. At this time, a known amount of multivalent antigen prepared by binding multiple nitro compounds of the same type as above to the carrier is added to the carrier, and this multivalent antigen and an unknown amount of the organic nitro compound to be measured, which is a monovalent antigen, are added to the carrier. By conducting a competitive reaction, the amount or presence or absence of the organic nitro compound can be identified from the suppression of the increase in intensity accompanying the antigen-antibody reaction. Turbidity can be measured by irradiating visible light, infrared light, laser light, etc., and measuring the transmitted light, reflected light, scattered light, etc. using a light receiving element.

Function: By this method, organic nitro compounds present in minute amounts in the air can be analyzed and detected in a short time and with a relatively small and lightweight device.

Example The measuring method of the present invention will be explained with reference to Examples.

Trinitrotoluene (T
NT) and select 2 from the power of Ig's TNT.
In order to collect and concentrate TNT from the air after 0.00 cm, a solution method was used, and the pH of about 10 mL was 7.
Bovine serum albumin (BSA) was concentrated approximately 102 times in a 0.0 phosphate buffer solution by passing approximately 15 air per minute for approximately 1 minute.
was combined using a known method, and mice were immunized with this antigen for about 5 weeks to produce polyclonal antibodies from the serum.

As an analysis method, a method of measuring visible light transmittance was selected as an example of a turbidity measurement method, and the same BSA as used for antibody production was selected as a carrier, and about 3 TNTs were bound to each BSA. 50 μL of this TNT-BSA (10 −6 M/L) was placed in each well of a 96-well microplate.

Next, the following experiment was conducted to obtain a calibration curve. For competitive reaction with TXT-BSA, 10-3
A known amount of free TNT of ˜10 −5 g/mL was added.

Then, the above-mentioned antibodies were added in an amount of 60 μl/7, slightly in excess of the amount of these antigens, and allowed to stand at room temperature for 1 minute to allow the antigen-antibody reaction to proceed.

Thereafter, the transmittance was measured at room temperature at a wavelength of 580 nm.

Therefore, the required measurement time was within about 3 minutes. The calibration curve thus obtained is shown in the figure.

The concentration of TNT showed a nearly proportional relationship between 10-3 and 10-5 g/mL.

Next, the same experiment was performed on the above sample obtained by collecting and concentrating it from the air, and as a result, the concentration in the air was 1.
It was found to be within the range of 0-5 to 10-7 g/mL. In other words, the time required for conventional equipment is 10 to 20 minutes, but using the method of the present invention, analysis can be completed in about 3 minutes.
It was found that it was detectable.

In the above examples, serum, ie, a polyclonal antibody, was used as the antibody, and therefore it had binding power to nitro compounds other than the above-mentioned TNT. For example, picric acid (0% binding cuff), nitroglycerin (30% binding cuff),
%), etc., can be analyzed and detected using this antibody.

Furthermore, when a monoclonal antibody, which generally has higher specificity and stronger binding strength than a polyclonal antibody, is used, it is difficult to detect other similar compounds, but it is possible to analyze and detect even lower concentrations. In this case, it has also been found that it is sufficient to use a mixture of several monoclonal antibodies.

In addition, BSA was used as a protein for antibody production.
In addition, hemocyanin (KLH) derived from keyhole limpet
However, similar effects were obtained.

Furthermore, as a method for measuring turbidity, near-infrared light and laser light can also be used in addition to visible light.

Effects of the Invention According to the present invention, extremely small amounts of nitro compounds can be collected and analyzed and detected with high sensitivity in a relatively short time.

[Brief explanation of the drawing]

The figure is a graph showing a calibration curve obtained by the measurement method according to the present invention.

Claims (4)

[Claims] (1) Chemically bonding the organic nitro compound to be detected with a protein, immunizing an animal with this to produce an antibody against the organic nitro compound, and then causing an antigen-antibody reaction between the organic nitro compound to be detected and the antibody. , a known amount of a multivalent antigen prepared by binding multiple nitro compounds of the same type as above to a carrier is added to the carrier, and this multivalent antigen and an unknown amount of an organic nitro compound to be measured, which is a monovalent antigen, are subjected to a competitive reaction. The method is characterized in that the amount of the organic nitro compound present or its presence or absence is identified by utilizing the fact that the increase in turbidity accompanying the antigen-antibody reaction is suppressed depending on the amount of the antigen to be measured. A method for detecting and analyzing organic nitro compounds. (2) The method for detecting and analyzing an organic nitro compound according to claim 1, characterized in that one or more types of monoclonal antibodies are used as the antibody. (3) The method for detecting and analyzing organic nitro compounds according to claim 1, characterized in that a polyclonal antibody is used as the antibody. (4) The method for detecting and analyzing organic nitro compounds according to claim 1, characterized in that a collection and concentration device using a solution method or an adsorption method is used to concentrate organic nitro compounds in the air.