JPH0130478B2 - - Google Patents

Info

Publication number: JPH0130478B2
Authority: JP; Japan
Prior art keywords: plasmid; dna; strain; medium; bacteria
Prior art date: 1981-03-06
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Expired

Application number

JP56032809A

Other languages

English (en)

Japanese (ja)

Other versions

JPS57146585A (en

Inventor

Shuichi Aiba

Tadayuki Imanaka

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

Shionogi and Co Ltd

Original Assignee

Shionogi and Co Ltd

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1981-03-06

Filing date

1981-03-06

Publication date

1989-06-20

1981-03-06 Application filed by Shionogi and Co Ltd filed Critical Shionogi and Co Ltd

1981-03-06 Priority to JP3280981A priority Critical patent/JPS57146585A/ja

1982-03-04 Priority to CA000397585A priority patent/CA1206108A/en

1982-03-05 Priority to GB8206511A priority patent/GB2095286B/en

1982-03-05 Priority to EP82301150A priority patent/EP0060663A3/en

1982-03-08 Priority to US06/356,150 priority patent/US4695546A/en

1982-09-10 Publication of JPS57146585A publication Critical patent/JPS57146585A/ja

1989-06-20 Publication of JPH0130478B2 publication Critical patent/JPH0130478B2/ja

Status Granted legal-status Critical Current

Links

239000013612 plasmid Substances 0.000 claims description 78
241000894006 Bacteria Species 0.000 claims description 41
238000000034 method Methods 0.000 claims description 27
230000009466 transformation Effects 0.000 claims description 18
241000193385 Geobacillus stearothermophilus Species 0.000 claims description 11
210000001938 protoplast Anatomy 0.000 claims description 11
239000000126 substance Substances 0.000 claims description 9
238000012546 transfer Methods 0.000 claims description 8
229930006000 Sucrose Natural products 0.000 claims description 7
CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 7
230000008929 regeneration Effects 0.000 claims description 7
238000011069 regeneration method Methods 0.000 claims description 7
239000005720 sucrose Substances 0.000 claims description 7
210000002421 cell wall Anatomy 0.000 claims description 4
230000015572 biosynthetic process Effects 0.000 claims description 2
238000003776 cleavage reaction Methods 0.000 claims description 2
108091008146 restriction endonucleases Proteins 0.000 claims description 2
230000007017 scission Effects 0.000 claims description 2
108020004414 DNA Proteins 0.000 description 45
239000000243 solution Substances 0.000 description 32
108010087702 Penicillinase Proteins 0.000 description 24
229950009506 penicillinase Drugs 0.000 description 22
FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 18
229930027917 kanamycin Natural products 0.000 description 18
229960000318 kanamycin Drugs 0.000 description 18
SBUJHOSQTJFQJX-NOAMYHISSA-N kanamycin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CN)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](N)[C@H](O)[C@@H](CO)O2)O)[C@H](N)C[C@@H]1N SBUJHOSQTJFQJX-NOAMYHISSA-N 0.000 description 18
229930182823 kanamycin A Natural products 0.000 description 18
229920001817 Agar Polymers 0.000 description 15
239000008272 agar Substances 0.000 description 15
238000004519 manufacturing process Methods 0.000 description 13
108090000623 proteins and genes Proteins 0.000 description 13
210000004027 cell Anatomy 0.000 description 12
244000063299 Bacillus subtilis Species 0.000 description 11
235000014469 Bacillus subtilis Nutrition 0.000 description 11
238000006243 chemical reaction Methods 0.000 description 10
239000012634 fragment Substances 0.000 description 10
241000193830 Bacillus <bacterium> Species 0.000 description 9
239000004098 Tetracycline Substances 0.000 description 9
229940079593 drug Drugs 0.000 description 9
239000003814 drug Substances 0.000 description 9
239000011780 sodium chloride Substances 0.000 description 9
229930101283 tetracycline Natural products 0.000 description 9
229960002180 tetracycline Drugs 0.000 description 9
235000019364 tetracycline Nutrition 0.000 description 9
150000003522 tetracyclines Chemical class 0.000 description 9
239000013611 chromosomal DNA Substances 0.000 description 8
244000005700 microbiome Species 0.000 description 8
241000588724 Escherichia coli Species 0.000 description 7
230000001580 bacterial effect Effects 0.000 description 7
239000013598 vector Substances 0.000 description 7
WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 6
239000008103 glucose Substances 0.000 description 6
239000000725 suspension Substances 0.000 description 6
241000194108 Bacillus licheniformis Species 0.000 description 5
102000053602 DNA Human genes 0.000 description 5
238000003794 Gram staining Methods 0.000 description 5
230000012010 growth Effects 0.000 description 5
LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 4
TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 4
102000016943 Muramidase Human genes 0.000 description 4
108010014251 Muramidase Proteins 0.000 description 4
108010062010 N-Acetylmuramoyl-L-alanine Amidase Proteins 0.000 description 4
LRHPLDYGYMQRHN-UHFFFAOYSA-N N-Butanol Chemical compound CCCCO LRHPLDYGYMQRHN-UHFFFAOYSA-N 0.000 description 4
239000002253 acid Substances 0.000 description 4
238000000246 agarose gel electrophoresis Methods 0.000 description 4
230000003698 anagen phase Effects 0.000 description 4
229940041514 candida albicans extract Drugs 0.000 description 4
238000012258 culturing Methods 0.000 description 4
230000002068 genetic effect Effects 0.000 description 4
238000010438 heat treatment Methods 0.000 description 4
229960000274 lysozyme Drugs 0.000 description 4
239000004325 lysozyme Substances 0.000 description 4
235000010335 lysozyme Nutrition 0.000 description 4
239000000203 mixture Substances 0.000 description 4
238000002360 preparation method Methods 0.000 description 4
238000011160 research Methods 0.000 description 4
239000012138 yeast extract Substances 0.000 description 4
206010059866 Drug resistance Diseases 0.000 description 3
KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 3
102000004190 Enzymes Human genes 0.000 description 3
108090000790 Enzymes Proteins 0.000 description 3
239000001888 Peptone Substances 0.000 description 3
108010080698 Peptones Proteins 0.000 description 3
201000004283 Shwachman-Diamond syndrome Diseases 0.000 description 3
241000191967 Staphylococcus aureus Species 0.000 description 3
239000007983 Tris buffer Substances 0.000 description 3
229960000723 ampicillin Drugs 0.000 description 3
AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 description 3
238000010367 cloning Methods 0.000 description 3
238000011109 contamination Methods 0.000 description 3
238000002298 density-gradient ultracentrifugation Methods 0.000 description 3
229940088598 enzyme Drugs 0.000 description 3
238000002474 experimental method Methods 0.000 description 3
239000007788 liquid Substances 0.000 description 3
235000019319 peptone Nutrition 0.000 description 3
229920001223 polyethylene glycol Polymers 0.000 description 3
239000006228 supernatant Substances 0.000 description 3
241000149420 Bothrometopus brevis Species 0.000 description 2
108091003079 Bovine Serum Albumin Proteins 0.000 description 2
102000012410 DNA Ligases Human genes 0.000 description 2
108010061982 DNA Ligases Proteins 0.000 description 2
108010042407 Endonucleases Proteins 0.000 description 2
102000004533 Endonucleases Human genes 0.000 description 2
QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 description 2
OFOBLEOULBTSOW-UHFFFAOYSA-N Propanedioic acid Natural products OC(=O)CC(O)=O OFOBLEOULBTSOW-UHFFFAOYSA-N 0.000 description 2
QIVBCDIJIAJPQS-UHFFFAOYSA-N Tryptophan Natural products C1=CC=C2C(CC(N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-UHFFFAOYSA-N 0.000 description 2
150000007513 acids Chemical class 0.000 description 2
239000003242 anti bacterial agent Substances 0.000 description 2
229940088710 antibiotic agent Drugs 0.000 description 2
229940098773 bovine serum albumin Drugs 0.000 description 2
239000000872 buffer Substances 0.000 description 2
239000006285 cell suspension Substances 0.000 description 2
238000005119 centrifugation Methods 0.000 description 2
238000001816 cooling Methods 0.000 description 2
ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 description 2
229910000396 dipotassium phosphate Inorganic materials 0.000 description 2
235000019797 dipotassium phosphate Nutrition 0.000 description 2
229960005542 ethidium bromide Drugs 0.000 description 2
229940088597 hormone Drugs 0.000 description 2
239000005556 hormone Substances 0.000 description 2
229910001629 magnesium chloride Inorganic materials 0.000 description 2
VZCYOOQTPOCHFL-UPHRSURJSA-N maleic acid Chemical compound OC(=O)\C=C/C(O)=O VZCYOOQTPOCHFL-UPHRSURJSA-N 0.000 description 2
239000011976 maleic acid Substances 0.000 description 2
239000012528 membrane Substances 0.000 description 2
150000002894 organic compounds Chemical class 0.000 description 2
230000009467 reduction Effects 0.000 description 2
230000000717 retained effect Effects 0.000 description 2
238000000926 separation method Methods 0.000 description 2
239000011734 sodium Substances 0.000 description 2
239000001509 sodium citrate Substances 0.000 description 2
NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 2
UCSJYZPVAKXKNQ-HZYVHMACSA-N streptomycin Chemical compound CN[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@H]1O[C@@H]1[C@](C=O)(O)[C@H](C)O[C@H]1O[C@@H]1[C@@H](NC(N)=N)[C@H](O)[C@@H](NC(N)=N)[C@H](O)[C@H]1O UCSJYZPVAKXKNQ-HZYVHMACSA-N 0.000 description 2
VZCYOOQTPOCHFL-UHFFFAOYSA-N trans-butenedioic acid Natural products OC(=O)C=CC(O)=O VZCYOOQTPOCHFL-UHFFFAOYSA-N 0.000 description 2
238000011426 transformation method Methods 0.000 description 2
230000001131 transforming effect Effects 0.000 description 2
LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 2
ZCYVEMRRCGMTRW-UHFFFAOYSA-N 7553-56-2 Chemical compound [I] ZCYVEMRRCGMTRW-UHFFFAOYSA-N 0.000 description 1
241000311115 Bacillus paralicheniformis ATCC 9945a Species 0.000 description 1
241000684564 Bacillus subtilis KCTC 1028 Species 0.000 description 1
241000192125 Firmicutes Species 0.000 description 1
102000014150 Interferons Human genes 0.000 description 1
108010050904 Interferons Proteins 0.000 description 1
239000007836 KH2PO4 Substances 0.000 description 1
239000008118 PEG 6000 Substances 0.000 description 1
229920002584 Polyethylene Glycol 6000 Polymers 0.000 description 1
239000002202 Polyethylene glycol Substances 0.000 description 1
108010059712 Pronase Proteins 0.000 description 1
229920002472 Starch Polymers 0.000 description 1
239000007984 Tris EDTA buffer Substances 0.000 description 1
239000011543 agarose gel Substances 0.000 description 1
238000004220 aggregation Methods 0.000 description 1
230000002776 aggregation Effects 0.000 description 1
125000001931 aliphatic group Chemical group 0.000 description 1
229930013930 alkaloid Natural products 0.000 description 1
108090000637 alpha-Amylases Proteins 0.000 description 1
102000004139 alpha-Amylases Human genes 0.000 description 1
229940024171 alpha-amylase Drugs 0.000 description 1
150000001413 amino acids Chemical class 0.000 description 1
150000001491 aromatic compounds Chemical class 0.000 description 1
KGBXLFKZBHKPEV-UHFFFAOYSA-N boric acid Chemical compound OB(O)O KGBXLFKZBHKPEV-UHFFFAOYSA-N 0.000 description 1
239000004327 boric acid Substances 0.000 description 1
229910052792 caesium Inorganic materials 0.000 description 1
TVFDJXOCXUVLDH-UHFFFAOYSA-N caesium atom Chemical compound [Cs] TVFDJXOCXUVLDH-UHFFFAOYSA-N 0.000 description 1
150000001720 carbohydrates Chemical class 0.000 description 1
230000008859 change Effects 0.000 description 1
229960005091 chloramphenicol Drugs 0.000 description 1
WIIZWVCIJKGZOK-RKDXNWHRSA-N chloramphenicol Chemical compound ClC(Cl)C(=O)N[C@H](CO)[C@H](O)C1=CC=C([N+]([O-])=O)C=C1 WIIZWVCIJKGZOK-RKDXNWHRSA-N 0.000 description 1
238000009833 condensation Methods 0.000 description 1
230000005494 condensation Effects 0.000 description 1
238000007796 conventional method Methods 0.000 description 1
238000005520 cutting process Methods 0.000 description 1
MTHSVFCYNBDYFN-UHFFFAOYSA-N diethylene glycol Chemical compound OCCOCCO MTHSVFCYNBDYFN-UHFFFAOYSA-N 0.000 description 1
238000007865 diluting Methods 0.000 description 1
VHJLVAABSRFDPM-QWWZWVQMSA-N dithiothreitol Chemical compound SC[C@@H](O)[C@H](O)CS VHJLVAABSRFDPM-QWWZWVQMSA-N 0.000 description 1
230000000694 effects Effects 0.000 description 1
238000001962 electrophoresis Methods 0.000 description 1
230000008030 elimination Effects 0.000 description 1
238000003379 elimination reaction Methods 0.000 description 1
ZMMJGEGLRURXTF-UHFFFAOYSA-N ethidium bromide Chemical compound [Br-].C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CC)=C1C1=CC=CC=C1 ZMMJGEGLRURXTF-UHFFFAOYSA-N 0.000 description 1
210000003527 eukaryotic cell Anatomy 0.000 description 1
238000000605 extraction Methods 0.000 description 1
238000005755 formation reaction Methods 0.000 description 1
150000002391 heterocyclic compounds Chemical class 0.000 description 1
230000000415 inactivating effect Effects 0.000 description 1
229910052500 inorganic mineral Inorganic materials 0.000 description 1
238000003780 insertion Methods 0.000 description 1
230000037431 insertion Effects 0.000 description 1
230000010354 integration Effects 0.000 description 1
229940079322 interferon Drugs 0.000 description 1
230000003834 intracellular effect Effects 0.000 description 1
239000011630 iodine Substances 0.000 description 1
229910052740 iodine Inorganic materials 0.000 description 1
238000006317 isomerization reaction Methods 0.000 description 1
125000000400 lauroyl group Chemical group O=C([*])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 description 1
239000006166 lysate Substances 0.000 description 1
239000003550 marker Substances 0.000 description 1
230000000813 microbial effect Effects 0.000 description 1
239000011707 mineral Substances 0.000 description 1
238000002156 mixing Methods 0.000 description 1
229910000402 monopotassium phosphate Inorganic materials 0.000 description 1
235000019796 monopotassium phosphate Nutrition 0.000 description 1
HFWWEMPLBCKNNM-UHFFFAOYSA-N n-[bis(hydroxyamino)methyl]hydroxylamine Chemical compound ONC(NO)NO HFWWEMPLBCKNNM-UHFFFAOYSA-N 0.000 description 1
108020004707 nucleic acids Proteins 0.000 description 1
150000007523 nucleic acids Chemical class 0.000 description 1
102000039446 nucleic acids Human genes 0.000 description 1
150000007524 organic acids Chemical class 0.000 description 1
235000005985 organic acids Nutrition 0.000 description 1
230000003647 oxidation Effects 0.000 description 1
238000007254 oxidation reaction Methods 0.000 description 1
GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 description 1
238000001556 precipitation Methods 0.000 description 1
108090000765 processed proteins & peptides Proteins 0.000 description 1
102000004196 processed proteins & peptides Human genes 0.000 description 1
239000000047 product Substances 0.000 description 1
230000000644 propagated effect Effects 0.000 description 1
238000006722 reduction reaction Methods 0.000 description 1
150000003839 salts Chemical class 0.000 description 1
238000013112 stability test Methods 0.000 description 1
239000008107 starch Substances 0.000 description 1
235000019698 starch Nutrition 0.000 description 1
150000003431 steroids Chemical class 0.000 description 1
229960005322 streptomycin Drugs 0.000 description 1
125000001424 substituent group Chemical group 0.000 description 1
239000000758 substrate Substances 0.000 description 1
-1 terpenes Chemical class 0.000 description 1
150000003505 terpenes Chemical class 0.000 description 1
235000007586 terpenes Nutrition 0.000 description 1
238000012360 testing method Methods 0.000 description 1
239000012137 tryptone Substances 0.000 description 1
241001624918 unidentified bacterium Species 0.000 description 1
241001515965 unidentified phage Species 0.000 description 1
238000005406 washing Methods 0.000 description 1

Landscapes

Micro-Organisms Or Cultivation Processes Thereof (AREA)

JP3280981A 1981-03-06 1981-03-06 Transforming method with insertion of plasmid Granted JPS57146585A (en)

Priority Applications (5)

Application Number	Priority Date	Filing Date	Title
JP3280981A JPS57146585A (en)	1981-03-06	1981-03-06	Transforming method with insertion of plasmid
CA000397585A CA1206108A (en)	1981-03-06	1982-03-04	Process for transforming microorganism by means of plasmid introduction
GB8206511A GB2095286B (en)	1981-03-06	1982-03-05	New vectors and a process for transforming certain microorganisms
EP82301150A EP0060663A3 (en)	1981-03-06	1982-03-05	A process for transforming certain microorganisms, vectors and their production, and a process for producing certain substances using said vectors
US06/356,150 US4695546A (en)	1981-03-06	1982-03-08	Transforming Bacillus stearothermophilus with plasmid vector pTB 19

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
JP3280981A JPS57146585A (en)	1981-03-06	1981-03-06	Transforming method with insertion of plasmid

Publications (2)

Publication Number	Publication Date
JPS57146585A JPS57146585A (en)	1982-09-10
JPH0130478B2 true JPH0130478B2 (da)	1989-06-20

Family

ID=12369154

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
JP3280981A Granted JPS57146585A (en)	1981-03-06	1981-03-06	Transforming method with insertion of plasmid

Country Status (1)

Country	Link
JP (1)	JPS57146585A (da)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
JPWO2017169751A1 (ja) *	2016-03-29	2019-02-07	イムラ・ジャパン株式会社	低温菌及び中温菌由来酵素の製造方法

1981
- 1981-03-06 JP JP3280981A patent/JPS57146585A/ja active Granted

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
JOURNAL OF GENERAL MICROBIOLOGY *

Also Published As

Publication number	Publication date
JPS57146585A (en)	1982-09-10

Publication	Publication Date	Title
Hershfield et al.	1974	Plasmid ColE1 as a molecular vehicle for cloning and amplification of DNA
US4695546A (en)	1987-09-22	Transforming Bacillus stearothermophilus with plasmid vector pTB 19
US4460689A (en)	1984-07-17	DNA Cloning vector TG1, derivatives, and processes of making
Heefner et al.	1984	Transformation of Clostridium perfringens
SU1200853A3 (ru)	1985-12-23	Способ конструировани гибридной плазмиды,содержащей генетический код термостойкой альфа-амилазы
US4508826A (en)	1985-04-02	Bacteriophage DNA cloning vector TG1 and microorganisms containing TG1
KR910001807B1 (ko)	1991-03-26	스태필로키나아제를 생산하는 유전자를 함유하는 새로운 대장균 및 스태필로키나아제의 제조법
CA1150169A (en)	1983-07-19	Broad host range small plasmid rings as cloning vehicles
Alikhanian et al.	1981	Transformation of Bacillus thuringiensis subsp. galleria protoplasts by plasmid pBC16
JPH0130478B2 (da)	1989-06-20
JPH0363357B2 (da)	1991-09-30
JPH0669368B2 (ja)	1994-09-07	酢酸菌の形質転換方法
US4376164A (en)	1983-03-08	Process for preparing broad host range small plasmid rings as cloning vehicles
JPH0229315B2 (da)	1990-06-28
US4418194A (en)	1983-11-29	DNA Fragments for forming plasmids
JPS58189199A (ja)	1983-11-04	枯草菌用のプラスミド・クロ−ニング・ベクタ−
EP0094506A2 (en)	1983-11-23	DNA cloning vector TG1, derivatives, products and processes
JPS61181374A (ja)	1986-08-14	中性プロテア−ゼの産生法
US4886754A (en)	1989-12-12	Recombinant bateriophage for heterologous cloning of bacillus microorganisms and method for its production
JPS59179081A (ja)	1984-10-11	プラスミドｐＴＰ１１０２
JPS61205484A (ja)	1986-09-11	新規プラスミド
JPS59135891A (ja)	1984-08-04	複合プラスミド
Hopkins	1970	for the degree of Doctor of Philosophy of the University of Edinburgh in the Faculty of Science".
JPH0571227B2 (da)	1993-10-06
JPS5945354B2 (ja)	1984-11-06	ネオマイシン、カナマイシン耐性プラスミドを保有する新規な微生物