JPH01501681A - ハイブリダイゼーションアッセイ及び該アッセイに使用されるベき手段 - Google Patents

ハイブリダイゼーションアッセイ及び該アッセイに使用されるベき手段

Info

Publication number: JPH01501681A
Authority: JP; Japan
Prior art keywords: water; group; nucleic acid; nucleotide sequence; polymers
Prior art date: 1986-10-17
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Pending

Application number

JP62506689A

Other languages

English (en)

Japanese (ja)

Inventor

イリコスキ，イリキ

フルスカイネン，ペルッツィ

ダレン，パトリック

サンド，クリスチャン

レブグレン，ティモ

キワトコウィスキ，マレック

Original Assignee

ワラック　オサケユイチア

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1986-10-17

Filing date

1987-10-16

Publication date

1989-06-15

1987-10-16 Application filed by ワラック　オサケユイチア filed Critical ワラック　オサケユイチア

1989-06-15 Publication of JPH01501681A publication Critical patent/JPH01501681A/ja

Status Pending legal-status Critical Current

Links

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PHSPJQZRQAJPPF-UHFFFAOYSA-N N-alpha-Methylhistamine Chemical compound CNCCC1=CN=CN1 PHSPJQZRQAJPPF-UHFFFAOYSA-N 0.000 description 1
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229910019142 PO4 Inorganic materials 0.000 description 1
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239000011230 binding agent Substances 0.000 description 1
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125000004057 biotinyl group Chemical group [H]N1C(=O)N([H])[C@]2([H])[C@@]([H])(SC([H])([H])[C@]12[H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C(*)=O 0.000 description 1
239000008280 blood Substances 0.000 description 1
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229940098773 bovine serum albumin Drugs 0.000 description 1
GDTBXPJZTBHREO-UHFFFAOYSA-N bromine Substances BrBr GDTBXPJZTBHREO-UHFFFAOYSA-N 0.000 description 1
229910052794 bromium Inorganic materials 0.000 description 1
239000011575 calcium Substances 0.000 description 1
229910052791 calcium Inorganic materials 0.000 description 1
238000004364 calculation method Methods 0.000 description 1
235000011089 carbon dioxide Nutrition 0.000 description 1
150000007942 carboxylates Chemical class 0.000 description 1
231100000260 carcinogenicity Toxicity 0.000 description 1
230000007670 carcinogenicity Effects 0.000 description 1
230000009920 chelation Effects 0.000 description 1
238000003508 chemical denaturation Methods 0.000 description 1
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238000004581 coalescence Methods 0.000 description 1
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125000001495 ethyl group Chemical group [H]C([H])([H])C([H])([H])* 0.000 description 1
NNMXSTWQJRPBJZ-UHFFFAOYSA-K europium(iii) chloride Chemical compound Cl[Eu](Cl)Cl NNMXSTWQJRPBJZ-UHFFFAOYSA-K 0.000 description 1
238000002474 experimental method Methods 0.000 description 1
230000002349 favourable effect Effects 0.000 description 1
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238000005259 measurement Methods 0.000 description 1
239000002609 medium Substances 0.000 description 1
238000000386 microscopy Methods 0.000 description 1
239000013642 negative control Substances 0.000 description 1
229910017604 nitric acid Inorganic materials 0.000 description 1
125000004433 nitrogen atom Chemical group N* 0.000 description 1
SJYNFBVQFBRSIB-UHFFFAOYSA-N norbornadiene Chemical compound C1=CC2C=CC1C2 SJYNFBVQFBRSIB-UHFFFAOYSA-N 0.000 description 1
238000000655 nuclear magnetic resonance spectrum Methods 0.000 description 1
229940046166 oligodeoxynucleotide Drugs 0.000 description 1
239000011368 organic material Substances 0.000 description 1
239000003960 organic solvent Substances 0.000 description 1
239000003208 petroleum Substances 0.000 description 1
125000001997 phenyl group Chemical group [H]C1=C([H])C([H])=C(*)C([H])=C1[H] 0.000 description 1
235000021317 phosphate Nutrition 0.000 description 1
239000008055 phosphate buffer solution Substances 0.000 description 1
UEZVMMHDMIWARA-UHFFFAOYSA-M phosphonate Chemical compound [O-]P(=O)=O UEZVMMHDMIWARA-UHFFFAOYSA-M 0.000 description 1
NBIIXXVUZAFLBC-UHFFFAOYSA-N phosphoric acid Substances OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 1
150000003013 phosphoric acid derivatives Chemical class 0.000 description 1
239000013612 plasmid Substances 0.000 description 1
229920000036 polyvinylpyrrolidone Polymers 0.000 description 1
239000001267 polyvinylpyrrolidone Substances 0.000 description 1
235000013855 polyvinylpyrrolidone Nutrition 0.000 description 1
239000002243 precursor Substances 0.000 description 1
125000006239 protecting group Chemical group 0.000 description 1
238000004445 quantitative analysis Methods 0.000 description 1
238000000163 radioactive labelling Methods 0.000 description 1
239000000941 radioactive substance Substances 0.000 description 1
239000000376 reactant Substances 0.000 description 1
238000012552 review Methods 0.000 description 1
PYWVYCXTNDRMGF-UHFFFAOYSA-N rhodamine B Chemical compound [Cl-].C=12C=CC(=[N+](CC)CC)C=C2OC2=CC(N(CC)CC)=CC=C2C=1C1=CC=CC=C1C(O)=O PYWVYCXTNDRMGF-UHFFFAOYSA-N 0.000 description 1
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238000012163 sequencing technique Methods 0.000 description 1
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XUARKZBEFFVFRG-UHFFFAOYSA-N silver sulfide Chemical compound [S-2].[Ag+].[Ag+] XUARKZBEFFVFRG-UHFFFAOYSA-N 0.000 description 1
150000003384 small molecules Chemical class 0.000 description 1
239000000779 smoke Substances 0.000 description 1
235000017557 sodium bicarbonate Nutrition 0.000 description 1
229910000030 sodium bicarbonate Inorganic materials 0.000 description 1
229910000033 sodium borohydride Inorganic materials 0.000 description 1
239000012279 sodium borohydride Substances 0.000 description 1
235000010267 sodium hydrogen sulphite Nutrition 0.000 description 1
229910052938 sodium sulfate Inorganic materials 0.000 description 1
235000011152 sodium sulphate Nutrition 0.000 description 1
239000002689 soil Substances 0.000 description 1
238000010561 standard procedure Methods 0.000 description 1
239000012086 standard solution Substances 0.000 description 1
239000007858 starting material Substances 0.000 description 1
239000012258 stirred mixture Substances 0.000 description 1
210000002784 stomach Anatomy 0.000 description 1
229910052717 sulfur Inorganic materials 0.000 description 1
239000011593 sulfur Substances 0.000 description 1
229920001059 synthetic polymer Polymers 0.000 description 1
229960003080 taurine Drugs 0.000 description 1
150000003512 tertiary amines Chemical class 0.000 description 1
MPLHNVLQVRSVEE-UHFFFAOYSA-N texas red Chemical compound [O-]S(=O)(=O)C1=CC(S(Cl)(=O)=O)=CC=C1C(C1=CC=2CCCN3CCCC(C=23)=C1O1)=C2C1=C(CCC1)C3=[N+]1CCCC3=C2 MPLHNVLQVRSVEE-UHFFFAOYSA-N 0.000 description 1
125000003396 thiol group Chemical group [H]S* 0.000 description 1
238000006177 thiolation reaction Methods 0.000 description 1
ZWZVWGITAAIFPS-UHFFFAOYSA-N thiophosgene Chemical compound ClC(Cl)=S ZWZVWGITAAIFPS-UHFFFAOYSA-N 0.000 description 1
231100000331 toxic Toxicity 0.000 description 1
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239000000052 vinegar Substances 0.000 description 1
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238000005406 washing Methods 0.000 description 1

Classifications

- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means

Landscapes

Chemical & Material Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Organic Chemistry (AREA)
Health & Medical Sciences (AREA)
Engineering & Computer Science (AREA)
Biochemistry (AREA)
Molecular Biology (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Wood Science & Technology (AREA)
Genetics & Genomics (AREA)
General Health & Medical Sciences (AREA)
Biotechnology (AREA)
Zoology (AREA)
Physics & Mathematics (AREA)
Biophysics (AREA)
Analytical Chemistry (AREA)
Bioinformatics & Cheminformatics (AREA)
General Engineering & Computer Science (AREA)
Microbiology (AREA)
Immunology (AREA)
Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Investigating Or Analysing Biological Materials (AREA)

JP62506689A 1986-10-17 1987-10-16 ハイブリダイゼーションアッセイ及び該アッセイに使用されるベき手段 Pending JPH01501681A (ja)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
SE8604436-9		1986-10-17
SE8604436A SE454781B (sv)	1986-10-17	1986-10-17	Hybridiseringsforfarande for detektion av polynukleotidsekvens

Publications (1)

Publication Number	Publication Date
JPH01501681A true JPH01501681A (ja)	1989-06-15

Family

ID=20365979

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
JP62506689A Pending JPH01501681A (ja)	1986-10-17	1987-10-16	ハイブリダイゼーションアッセイ及び該アッセイに使用されるベき手段

Country Status (7)

Country	Link
US (1)	US5256535A (de)
EP (1)	EP0288513B1 (de)
JP (1)	JPH01501681A (de)
AT (1)	ATE85367T1 (de)
DE (1)	DE3784058T2 (de)
SE (1)	SE454781B (de)
WO (1)	WO1988002784A1 (de)

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1986
- 1986-10-17 SE SE8604436A patent/SE454781B/sv not_active IP Right Cessation
1987
- 1987-10-16 AT AT87907035T patent/ATE85367T1/de not_active IP Right Cessation
- 1987-10-16 WO PCT/SE1987/000474 patent/WO1988002784A1/en not_active Ceased
- 1987-10-16 DE DE8787907035T patent/DE3784058T2/de not_active Expired - Lifetime
- 1987-10-16 EP EP87907035A patent/EP0288513B1/de not_active Expired - Lifetime
- 1987-10-16 US US07/207,159 patent/US5256535A/en not_active Expired - Lifetime
- 1987-10-16 JP JP62506689A patent/JPH01501681A/ja active Pending

Also Published As

Publication number	Publication date
SE8604436L (sv)	1988-04-18
SE454781B (sv)	1988-05-30
US5256535A (en)	1993-10-26
EP0288513A1 (de)	1988-11-02
WO1988002784A1 (en)	1988-04-21
DE3784058T2 (de)	1993-05-19
DE3784058D1 (de)	1993-03-18
ATE85367T1 (de)	1993-02-15
SE8604436D0 (sv)	1986-10-17
EP0288513B1 (de)	1993-02-03

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