JPH02129103A - Solution composition for treatment of cultivated plant - Google Patents

Abstract

PURPOSE:To obtain the subject composition stably obtaining excellent activities such as yield increase, growth promoting and resistance to stress by comprising a mixed solution containing lower aliphatic alcohol, amide-based polar solvent, etc., water-soluble polymer, spreading agent and water and brassinorides. CONSTITUTION:1 pt.wt. mixed solution composed of 50-98wt.% lower aliphatic alcohol (especially ethanol), 1-25wt.% solvent selected from amide-based polar solvent (e.g., DMF) and DMSO, 1-25wt.% water-soluble polymer (especially polyethylene glycol) and water is mixed with 0.002-2 pts.wt. spreading agent, then resultant mixed solution is combined with brassinorides expressed by the formula to afford the subject composition. Effect of the brassinorides to plant is improved by using a solution added with specific solvent and polymeric compound such as above. Application of the subject composition to plant is selected as dipping, foliage application or watering to soil, etc., according to the objective.

Description

DETAILED DESCRIPTION OF THE INVENTION a. Field of Industrial Application The present invention relates to a liquid composition for treating cultivated plants containing brassinolides. More specifically, the present invention relates to a brassinolide-containing liquid composition having a novel composition with enhanced activity against plants.

b. Prior Art Research on growth regulation, particularly growth promotion, of cultivated plants has been rapidly and extensively advanced in recent years, focusing on major grains. Among them, plant hormones such as auxin, gibberellin, cytokinin, abscisic acid, and ethylene have already been put into practical use, and many new plant hormones have been proposed.

Brassinolide is one of the plant growth promoting substances that has been proposed in recent years. Brassinolide was discovered in the pollen of Brassica napus by Mitschel and Mandava et al. [Hitchell, J.W.

N., Handava, J.F., Worley and.
J.R.Primer, Nature225, 106
5 (1970)]. Through research by Mitchell et al., it was confirmed that brassinolide has an extremely significant elongation effect when applied to young kidney bean shoots. Subsequently, Mandarva et al. confirmed and announced that this brassinolide has the following chemical structure [Natu
RE, 281.216 (1979) 1. Brassinolide is a type of steroid with the above-mentioned complex structure, and various attempts have been made to chemically synthesize it, as well as the development of its analogs and its physiological activity. Research is also being conducted.

For example, analogues of the brassinolide, such as (22S, 23S) homobrassinolide, which is an optical isomer and a substituted product, and epi-brassinolide, which is a stereoisomer (24R-methyl); Many have also been proposed, and it is also known that they have almost the same activity as brassinolide, although to a lesser extent.

The physiologically active effects of conventionally known brassinolides on cultivated plants have been investigated and reported.

Grains such as rice, corn, soybeans, wheat, tomatoes, and Kirari, to name a few.

It is known to have a yield-increasing effect on vegetables, a growth-promoting effect on rice and corn, a stress-resistant effect against deterioration of the environment during life, and an effect on promoting fruit set in navel oranges.

On the other hand, brassinolides are extremely poorly soluble in water, and when applied to cultivated plants, they are first dissolved in lower alcohols to form a solution, which is then diluted with water. Further, in this case, a spreading agent used in agricultural chemicals is usually added.

The effects of the brassinolides mentioned above on various cultivated plants can be determined by diluting a solution of brassinolide in a lower alcohol such as ethyl alcohol with water, and adding a spreading agent in the usual case. Confirmed by spraying or dipping plants.

However, according to the research of the present inventors, the effect of brassinolides on plants is influenced by the composition of the solution in which brassinolides are dissolved. It was found that even more excellent activity could be stably obtained by doing so.

C9 Object of the Invention An object of the present invention is to provide a novel brassinolide-containing liquid composition for treating cultivated plants that stably provides excellent activity.

d9 Structure of the Invention According to the present invention, the components outside of the wood removal include (il lower aliphatic alcohol 50-98% by weight, (ii) at least one solvent selected from amide polar solvents and dimethyl sulfoxide. 1-2 only 1%, (iil water-soluble polymer 1-25
% by weight of a mixed liquid containing 0.002 to 2 parts by weight of an IVL spreading agent (non-aqueous component) and M brassinolides. Provided is a liquid composition for treating cultivated plants that stably provides excellent activity.

In the composition of the present invention, in addition to the conventionally known composition, (iii) at least one solvent selected from amide polar solvents and dimethyl sulfoxide, and (iii) a water-soluble polymer are blended in the above proportions, By using these components in combination, the activity of brassinolides is significantly improved compared to those with conventionally known compositions,
Furthermore, an extremely excellent advantage is obtained in that the expression of the activity is stabilized.

The brassinolides used in the present invention include those having a brassinosteroid matrix and having the same physiological activity as brassinolide.

Thus, in the present invention, the brassinolides include:
Brassinolide itself is most preferred, but its stereoisomers,
It may also be an analogue.

Brassinolide is called 2α,3α,22R,23R-tetrahydroxy-243-methyl-B-homo-7=oxa-5α-cholestan-6-one, and has a melting point of 274 to 275° expressed by the chemical formula below. It is a compound of C and is sparingly soluble in water.

Homobrassinolite and epibrassinolide can also be used as analogs of brassinolide, although they are less active than the above-mentioned brassinolide [for example, [This Month's Agriculture J]
See June 1988 issue, pp. 65-69].

As mentioned above, brassinolides themselves are sparingly soluble in water, but lower aliphatic alcohols or (i) to (
It is soluble in a mixed solution of ivl, and the liquid composition of the present invention can be prepared by dissolving it therein.

Hereinafter, each component (i) to (iv) constituting the composition of the present invention will be described.
) will be explained.

Examples of the lower aliphatic alcohol in (1) include methanol, ethanol, n-propanol, IsO propazol, n-butanol, 1so-butanol, 5e
Examples include c-butanol, and ethanol is particularly preferred. These lower aliphatic alcohols are (i
It is used in an amount of 50 to 98% by weight based on the total amount of each component (ii) and (iii), preferably 60% by weight.
The range is 95% by weight.

The solvent (ii) is selected from amide polar solvents and dimethyl sulfoxide (DMSO). Examples of amide polar solvents include dimethylformamide (DMF), N
-Methylpyrrolidone (NMP).

Examples include dimethylacetamide (DMAA), and D
~1'' or NMP is preferred, and NMP is particularly preferred. used in range.

Examples of the water-soluble polymer (ii) include polyethylene glycol (PEG), polypropylene glycol,
Examples include polyalkylene glycols such as polybutylene glycol, polyvinylpyrrolidone and polyvinyl alcohol, or copolymers thereof, and polyethylene glycol is particularly preferred. These polyalkylene glycols can be advantageously used in the range of 300 to 5,000000, but if the molecule is soluble in water or lower aliphatic alcohol,
There are no particular restrictions. These water-soluble polymers are (i)
(ii).

The total hanging weight of (iii) is used in a range of 1 to 25% by weight, preferably in a range of 2 to 20% by weight.

On the other hand, the spreading agent (iv) is a plant growth regulator.

Spreading agents that are commonly used in agrochemical compositions such as herbicides, insecticides, fungicides, and fungicides can be used. Specific examples of the spreading agent include polyoxyalkylene ether systems such as polyoxyethylene dialkyl ether, polyoxyethylene alkyl allyl ether, and polyoxyethylene diallyl ether; for example, polyoxyethylene dialkyl ester, polyoxyethylene alkyl allyl ester; , polyoxyalkylene diester systems such as polyoxyethylene diallyl ester, e.g. sodium dinaphthyl methanesulfonate,
Calcium lignin sulfonate.

Examples include sulfonate salts such as dialkyl sulfonaccinates. These spreading agents are usually commercially available for agricultural chemicals and can be used, or two or more of them can also be used in combination. The spreading agent is based on the weight of the components other than wood removal, and is calculated as above (il, (ii)
), 0.002 per 1 mm part of the composition consisting of C town.
-2 parts by weight, preferably 0.003 to 1.8 parts by weight, particularly preferably 1.1 to 1.7 parts by weight. A large amount of spreading agent has the advantage that it lasts for a long time on the plant surface.

The composition of the present invention is formed from a liquid mixture consisting of (i) to (iii) above, (iv) a spreading agent, and (V) brassinolides.Brassinolides are extremely sensitive to plants. Since it exhibits its activity, its content only needs to be extremely small.

Therefore, the content ratio of brassinolides in the composition varies very slightly. In general, 'l X 10-51)l)m~i
o, oo.

ppm, preferably 1 x 110-5pp ~ 50
It is sufficient if it is within the range of 00 ppm.

There is no problem even if the composition of the present invention further contains water in addition to the above composition. Generally, when applied to plants, the composition of the present invention is used after being diluted with water.

In that case, the water is 50 to 7,000 times the weight of the composition,
Lima L < Li2O2 ~ 5,000 ffi It is used after being diluted with Nagisa times the amount of water. Even compositions diluted with water are included in the present invention as long as the components from which water has been removed fall within the range specified in the present invention.

As mentioned above, the composition of the present invention is used after being diluted with water, and the concentration of brassinolides in the diluted composition is generally 1 x 10-8 p.
pm to 500 ppm, preferably 1X10'
ppm to 50 ppm, particularly preferably 1X10
-6 ppm to 10 ppm is advantageous.

The concentration of the brassinolides depends on the time of treatment.

The suitable range depends on the treatment method, number of treatments, type of plant, purpose, etc.

e8 Effects of the Invention By applying the brassinolide-containing composition according to the present invention to various plants, the brassinolide-containing composition, in particular, can be applied to various plants. can exhibit superior physiological activity compared to

For example, it has a yield-increasing effect, growth-promoting effect, and stress-resistance effect on major grains such as soybeans, wheat, rice, corn, and potatoes, and it also has a similar yield-increasing effect and growth-promoting effect on tomatoes, kirari, and beans. This significantly improves stress resistance.

In addition, citrus fruits, grapes, persimmons, peaches, apples,
When applied to fruit trees such as pears, it not only increases yield, promotes growth, and improves stress resistance, but also improves fruit set rate, prevents fruit from falling, and suppresses defoliation.

The method for treating cultivated plants using the brassinolide-containing composition according to the present invention is directed to target plants.

Preferred modes vary depending on the purpose, but for example, soaking of seeds and seed potatoes, spraying on foliage, soil irrigation and foliar spraying, spraying on flower clusters, injection treatment, etc. are adopted, and these may be used in combination or alone. It can be processed several times. In particular, treatment with 9 15 foliar sprays on foliage gives good results.

f. Examples The present invention will be described in detail below with reference to Examples and Comparative Examples, but it goes without saying that the present invention is not limited to these.

In the composition of each mixed liquid below, the following abbreviations mean the following, respectively.

BR; Brassinolide
DMF Nidimethylformamide NMP: N-methylpyrrolidone PEG100O: Polyethylene glycol with an average molecular weight of 1000 EtOH: Ethanol Panno ■ (spreading agent) [manufactured by Yamamoto Nohyaku]: Tokubanno ■
(Spreading agent) [Manufactured by Yamamoto Nohyaku]: Neoesterin ■ (Spreading agent) [Manufactured by Kumiai Chemical] Examples 1-2. Comparative Example 1 [I] Preparation of mixed liquids Mixed liquids having the following compositions were prepared.

(1) To the mixed solution -1 (blank) (2) Mixed solution B-1 (BRII degree 1 Di) m) Dilute 1 g of the preparation with the following composition with water to make 500 d, and add Panno ■ 1.5 Di. It was prepared using

(3) Mixed liquid B-2 (BRI degree 'l X 10-21)
l) III) Dilute 1 g of the preparation (5) above 100 times with a diluent having the composition below, dilute that 1 g with water, and make 500 d.
and further added Panno@1.5 rnl.

One unit was a branch with about 10 flowers on the test tree, and three branches were treated with each mixed solution. Each mixture was sprayed onto flowers and leaves using a hand spray during the full bloom period.

On November 24th (harvest period), the juice percentage, acidity,
The sweetness ratio was investigated. The results are shown in Table 1 below.

Table 1 [II] Growth test (1988) The test was carried out in 1988 using a 27-year-old orange mandarin tree "Kochu" as a test tree.

On March 2nd, fertilizer was applied using a composite compound consisting of 1 of P:N: and 10K of component m per 10a (62.5g of each of P and N per tree).3 Month 5
I pruned it on the day.

From the above Table-1, mixture B-1, which is the composition of the present invention,
It can be seen that B-2 is effective in improving the fruit juice percentage, reducing acidity, and improving sweetness ratio.

~5 All 7 Examples Comparative Examples 2-4 [I] Preparation of mixed solution Mixtures having the following compositions were prepared.

Note that the Tokubanno ■ used was diluted 10 times with water.

(1) Mixed liquid C-1 (blank) [Tokubanno■ 1.50d (4) Mixed liquid C-4 (BRI degree 1 x 10-41) l
) m) (5) Mixed liquid D-2 (t3R concentration 1 pi) m)
It was prepared by diluting the formulation 1d with the following composition with 499-ml water and adding Tokubanno o 1.5d.

(2) Mixture C-2 (BRyA concentration 1 ppm) (6) Mixture D-3 (SR concentration 1 x 10-2 ppm) Preparation 1d from (5) above is diluted 100 times with a diluent having the following composition. 1 d was diluted with 499 d of water, and 1.5 d of Tokubanno ■ was added to the colander.

(3) Mixed liquid C-3 (BRIi degree 1 x 10-2 p
pm) (7) Mixture D-4 (SR concentration 1 x 10'
l) l) m) Add the formulations o and oimi of (5) above to (6) above.
) diluted 10,000 times with diluent, and 499
Inl of water was added, and 1.5 d of Tokubanno ■ was added to the colander.

[II] Growth test The test was conducted in 1986 using a 27-year-old mandarin orange "Genji" as a test tree.

On March 2nd, it was desired to fertilize N:P: per 10a using a compound compound consisting of 10Kq of each component (N:P:: 62.5 (l) per tree). March 5
I pruned it on the day.

A branch on which about 10 flowers had appeared on the test tree was defined as one branch, and three branches were treated with each mixed solution. May 21st (
During the full bloom period, each mixture was applied to flowers and rapeseed by hand spray.

On July 29th (69 days after the full bloom period), the number of leaves and fruit set in each treated plot was checked, and the number of leaves per fruit set was calculated. The results are shown in Table 2 and Table 3 below.

The results shown in Table 2 are the results using the conventional brassinolide-containing composition, and the results shown in Table 3 are the results using the composition according to the present invention. - Comparing the results in Table 2 and Table 3, even when using the same concentration of brassinolide, the results in Table 3 have a higher ratio of leaf number/plant number than Table 2. It can be seen that is small. In other words, it is understood that the number of fruits set for the same number of leaves is large.

table table Example 6 A processing solution having the following composition was prepared.

(1) Mixture E; B R20,7mQ NMP 20g (2) Mixture F P E G 1000 100 (1 Neoesterin 200Q) 100 fruits (flowers) were selected and used.

The treatments were treated 3 days before flowering (A) and treated 3 days after flowering (B).
) Evaluation was conducted by setting up three treatment groups (C) treated on day 0 after flll. The flowering period (full bloom date) was May 26th.

In each treatment area, the spray was applied to sufficiently wet the buds and fruits. A second spraying was carried out in each treated plot 4 days later.

The results are expressed as the fruit drop rate for treated fruits (flowers), and the survey was conducted from 16 to 20 days after flowering, with the first survey being conducted 3 days after flowering. The results are shown in Table 4 below.

The above mixed solution F2.25d for the above mixed solution E10mfl
was added and diluted with 51 parts of water before use.

[II] Growth test (1988) A test was conducted using flat-kerneled persimmon trees planted in a field in a fruit tree experimental station in Ibaraki Prefecture. Each treatment area has 50 fruits (flowers)
These were selected as test plots. Table of Contents Seasonal Treatment of Brassinolide is Kakihira From the results shown in Table 4 above, by spraying the composition of the present invention during the bud set stage, flowering stage, and young fruit stage, the percentage of physiological fruit drop after spraying can be reduced. It can be seen that this can clearly be suppressed.

Claims (1)

[Scope of Claims] Components other than water include (i) 50 to 98% by weight of lower aliphatic alcohol, (ii)
) 1 to 25% by weight of at least one solvent selected from amide polar solvents and dimethyl sulfoxide; (iii) 1 to 25% by weight of a water-soluble polymer; (iv) The weight of the adhesive other than water is 0.002
A liquid composition for treating cultivated plants comprising a mixture containing up to 2 parts by weight, and (v) brassinolides.