JPH0243532B2 - - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION (Technical Field) The present invention relates to a sample container used when measuring the state of fluorescence, etc. of a fluid sample while maintaining it at a constant temperature and stirring the sample.

(Prior Art) An apparatus shown in FIG. 1 has been proposed as an apparatus for measuring chemiluminescence during phagocytosis of leukocytes.

To measure the chemiluminescence of fluid leukocytes, the sample must be placed in the dark, kept at a constant temperature, and stirred.

When polymorphonuclear leukocytes begin predation and sterilization of bacteria etc., an increase in cyanogen-resistant oxygen consumption occurs. In the oxidative sterilization process, ground state oxygen generates active oxygen, which performs sterilization.

This acts on substrates such as bacteria, producing intramolecularly excited oxygen. Light is produced when this energy falls to the ground state.

By observing this light, it is possible to know the relationship between white blood cells and specific bacteria.

The sample plate 6 containing the sample 7 is a hot plate 5
The hot plate 5 is heated by a heater 3 supplied with power from a heater power source 4.

A rotor 8 for stirring the magnetic material is housed in a sample dish 6 containing a sample 7, and this rotor 8 is rotated by a pair of permanent magnets 2a and 2b rotated by the shaft of the motor 1. Stir sample 7. A dark box 9 with a shutter is placed above the sample dish 6, and a photomultiplier tube 10 is placed inside the dark box 9.

The photomultiplier tube 10 is supplied with an operating voltage from an external high-voltage power source 22, and multiplies the chemiluminescence of the sample and outputs it. The output of the photomultiplier tube 10 is counted and displayed by an external counter 21.

The main parts of the apparatus are housed in a dark room 20.

This is because unless the main part of the apparatus is housed in the dark room 20, weak fluorescence cannot be observed.

Although chemiluminescence and the like of the sample 7 have been observed and measured using the above-mentioned device, there are the following problems.

The light emitting area is limited by the stirring rotor 8 itself. An unnecessary rotor is included in the field of view of the photomultiplier tube 10.

Decomposition of the sample 7 may occur due to friction between the rotor 8 and the sample plate 6 and direct contact between the rotor 8 and the sample 7.

It is necessary to clean the rotor 8 every time the sample 7 is replaced.

In particular, in the measurement of chemical fluorescence as mentioned above, it is necessary to analyze changes in fluorescence emission over time due to reactions, so it is necessary to analyze changes in fluorescence emission over time due to reactions, so
There is a strong desire to suppress fluorescence as much as possible.

(Object of the Invention) An object of the present invention is to provide a sample container that solves the above-mentioned problems.

(Description of structure) In order to achieve the above-mentioned object, the sample container according to the present invention accommodates a sample dish containing a fluid sample in a constant temperature container containing a constant temperature liquid, and observes the sample while stirring the sample. In the sample container for
A discontinuous structure is provided on the bottom surface so as to stir the sample against the flow inertia of the sample housed in the sample dish, and a rotatable magnetic body is provided integrally with the sample dish. By applying a magnetic field that generates a rotational force to the magnetic body from the outside, the sample dish is rotated and the sample inside is stirred.

(Description of Examples) The present invention will be described in more detail below with reference to the drawings.

FIG. 2 is a sectional view showing an embodiment of the sample container according to the present invention.

FIG. 3 is a perspective view showing a broken example of the sample dish.

The motor 19 is a motor capable of forward and reverse rotation,
A rotary table is fixed to the shaft of the motor 19, and a pair of permanent magnets 18a and 18b are fixed on the rotary table.

Support stand 17 fixed in a constant temperature room (not shown)
A thermostatic container 16 filled with a bath for maintaining the sample at a constant temperature is placed above the thermostatic container 16 concentrically with the motor 19 . A frame 15 is arranged at the center of the constant temperature container 16.

Permanent magnets 14a and 14b are fixed to the bottom surface of the sample plate support 13, respectively, and are magnetically coupled to the pair of permanent magnets 18a and 18b on the motor side, respectively.

The frame 15 includes the sample plate support 13 and the permanent magnet 1.
It rotatably supports the assembly of 4a and 14b.

The sample plate 12 is placed on this sample plate support stand 13.

Due to the weight of the sample 11 placed in the sample plate 12, the sample plate 12 is coupled to the sample plate support 13 by frictional force and rotated together with the sample plate support 13.

As shown in FIG. 3A, the sample dish 12 is provided with a convex rib 12a on the bottom surface.

The height of this convex rib 12a is such that the sample 11 is
2, the convex rib 12a is below the liquid level.

The sample dish 12 in this embodiment has an inner diameter of 50 mm and 2
The height of the convex rib 12a is set slightly lower than 1 mm in order to accommodate a cc sample.

Appropriate holes are provided in the support stand 15 and the sample plate support stand 13 to promote the flow of the bath and eliminate temperature imbalance depending on the location of the bath.

Other configurations for observing the fluorescence of a sample using this sample container are the same as those described above with reference to FIG.

When the sample pan 12 containing the sample 11 is arranged at the position shown in the figure after adjusting the surrounding temperature environment and rotating the motor 19 in forward and reverse directions, the sample pan 12 is rotated, but the sample pan 12 accommodated in the sample pan 12 is rotated. Since sample 11 initially tries to maintain its initial position, sample 11
A relative rotation occurs between the sample plate 12 and the sample plate 12.

The sample 11 is agitated by colliding with a protrusion 12a that is discontinuous with the bottom surface of the sample dish 12.

When the viscosity of the sample 11 generates fluid inertia in the sample rotating with the sample plate 12, when the sample plate 12 is turned over, the sample 11 is further stirred.

Various modifications can be made to the embodiments described above within the scope of the present invention.

Although an example of a protruding strip is shown as a discontinuous structure for stirring the sample 11 against the flow inertia of the sample housed in the sample dish 12, it is possible to use a plurality of protrusions to further increase the stirring effect. You can also do it.

Contrary to the above-mentioned protrusions, there are grooves 1 as shown in FIG.
A similar stirring effect can be obtained with 2b. Even in this case, by providing a large number of grooves, the stirring effect can be further increased. Of course, it is also possible to combine protrusions and grooves.

It is also possible to fix a permanent magnet directly to the sample dish.

(Explanation of effects) As explained above, the sample container according to the present invention has
Since the sample plate containing the sample itself is configured to be able to be stirred by rotating it from the outside, there is no need to provide stirring means inside the sample plate that would obstruct the field of view for observation.

As a result, in the case of weak luminescence measurement of a sample, which was a problem with the conventional apparatus shown in FIG. 1, the blocking of light by the rotor and the influence of friction on the sample on the sample can be completely eliminated.

Additionally, cleaning and handling of the sample dish has become much easier than with conventional devices.

The container according to the present invention can be widely used for observing the luminescence of oil, sewage, etc. in addition to observing the white blood cells.

[Brief explanation of drawings]

FIG. 1 is a schematic diagram showing a conventional apparatus for stirring a sample and observing the fluorescence emission of the sample. FIG. 2 is a sectional view showing an embodiment of the sample container according to the present invention. FIG. 3 is a perspective view showing a broken example of the sample dish. DESCRIPTION OF SYMBOLS 1...Motor, 2a, 2b...Pair of permanent magnets, 3...Heater, 4...Power source, 5...Hot plate, 6...Sample plate, 7...Sample, 8...Rotor, 9... ...Dark box with shutter, 10...Photomultiplier tube, 11...Sample, 12...Sample dish, 12a...
...Convex strip, 12b...Concave groove, 13...Sample plate support stand, 14a, 14b...Pair of permanent magnets, 15...
...Support frame, 16... Constant temperature container, 17... Mounting table,
18a, 18b...Permanent magnet, 19...Motor,
20... Dark box, 21... Counter, 22... High voltage power supply.

Claims (1)

[Scope of Claims] 1. A sample container for observing a sample while stirring the sample by placing a sample dish containing a fluid sample in a constant-temperature container containing a constant-temperature liquid, wherein: A discontinuous structure is provided on the bottom to agitate the sample against the flow inertia of the sample.
By providing a rotatable magnetic body integrally with the sample dish, and applying a magnetic field that generates a rotational force from the outside to the magnetic body using an external magnetic field generating means,
A sample container characterized in that the sample dish is configured to be rotated to stir the sample inside. 2. The sample container according to claim 1, wherein the discontinuous structure provided on the bottom surface of the sample dish is one or more convex shapes in a direction substantially perpendicular to a direction of relative rotation occurring between the sample and the sample dish. 3. The sample container according to claim 1, wherein the discontinuous structure provided on the bottom surface of the sample dish is one or more grooves in a direction substantially perpendicular to the direction of relative rotation occurring between the sample and the sample dish. 4. The sample container according to claim 1, wherein the sample accommodated in the sample dish is a substance that generates weak fluorescence. 5. The external magnetic field generating means alternately generates, in a magnetic body rotatable together with the sample plate, a magnetic field that rotates the sample plate in a first direction and a second direction. sample container.