JPH0317845Y2 - - Google Patents

Description

[Detailed explanation of the idea] [Industrial application field] The present invention relates to an artificial epidermal blister preparation device.

BACKGROUND OF THE INVENTION The most frequently used procedure for harvesting skin tissue is trepanning. Sampling skin using this trepan is technically simple and has become indispensable for diagnosing skin diseases. However, punch biopsy using trepanation involves stress on the examinee, such as local anesthesia using xylocaine and trauma and scarring of the examined area. Further, even when only epidermal tissue (cells) is required experimentally, punch biopsy has the drawback of having to collect tissue including the dermis.

In response to this, an artificial epidermal blister preparation device has been proposed. This is suitable when only the epidermal tissue is required, and a syringe or plastic tube is used to apply 150 to 200 mHg to the test skin.
The aim is to artificially create epidermal blisters by applying negative pressure.

[Problems to be solved by the invention] However, with conventional artificial epidermal blister preparation devices, there are problems such as the difficulty in quantitatively monitoring the degree of vacuum pressure acting on the test skin. There is.

SUMMARY OF THE INVENTION An object of the present invention is to solve these problems and provide an artificial epidermal blister preparation device that has advantages such as being able to easily monitor the degree of vacuum pressure.

[Means for Solving the Problems] In order to achieve the above object, the present invention provides a vacuum cup that is formed of a translucent container and whose opening side can be attached to the skin surface, and this vacuum cup. a suction device capable of vacuum suctioning the inside of the device, and a monitoring unit for the vacuum pressure generated by the suction device;
The structure includes an adjustment section for adjusting the vacuum pressure.

[Function] According to this configuration, when the vacuum cup is attached to the skin surface and the inside thereof is vacuum-sucked by the suction device and a certain period of time has elapsed, the skin surface corresponding to the opening of the vacuum cup is removed. Blisters are created artificially. At this time, the degree of vacuum within the vacuum cup is monitored by the monitor section. As the monitor section, it is preferable to use a vacuum gauge or the like.
The adjustment section also adjusts the negative pressure within the vacuum cup. As the adjustment section, it is preferable to use a pressure regulating valve or the like. Furthermore, since the vacuum cup is formed of a translucent container, the formation of blisters can be observed from the outside over time.

[Example] In FIG. 1, 1 is a vacuum cup that can be attached to the skin surface where an artificial epidermal blister is to be produced. The interior of the vacuum cup 1 is communicated via a vinyl tube 2 to a vacuum pump 3 as a suction device. Vacuum pump 3 is installed in tube 2.
A vacuum gauge 4 as a monitor section for monitoring the negative pressure inside the vacuum cup 1 which is reduced by the vacuum cup 1, and a pressure control valve 5 as a control section for adjusting the pressure of the negative pressure are provided. .

As shown in Figures 2 to 4, the vacuum cup 1
has a cup body 6. The cup body 6 is formed into a cup shape from a translucent methacrylic resin or the like, so that the formation of blisters can be observed from the outside over time. 7 is a connection port with the tube 2, which communicates with the internal space 8 of the cup body 6. A flange 9 for contacting the skin to be examined is formed on the open end side of the cup body 6, and a stepped portion 10 formed at the center of the opening end for contacting the skin to be examined. disc-shaped skin surface adapter 11
is inserted. The surface of the skin adapter 11 is flush with the end face of the contact flange 9, and the stepped portion 10 is provided with an O-ring 1 for sealing between the skin adapter 11 and the cup body 6.
2 is provided. The skin surface adapter 11 is
It is made of a methacrylic resin having the same translucency as the cup body 6, and has three through holes 13 at equal pitches around its center and communicating with the internal space 8 of the cup body 6.

Specifically, the contact surface flange 9 of the cup body 6
is formed so that its diameter is 50 mm and the flange thickness is 2 mm. Further, the outer diameter and overall height of the cup body 6 are both 40 mm. The skin surface adapter 11 has a diameter of 30 mm and a thickness of 3
It is formed in mm. The skin surface adapter 11 is
Two types are available, one with three through holes 13 having a diameter of 6 mm and one with a diameter of 10 mm, and can be used depending on the situation.

As shown in FIG. 4, a fixing band is attached to the contact flange 9 using a metal fitting 14.
The fixing band 15 allows the vacuum cup 1 to be fixed to a skin part to be examined, such as an arm.

In such a configuration, when creating an epidermal blister artificially, first apply a thin layer of petroleum jelly or oil to the surface that contacts the skin surface, that is, the surface of the contact surface flange 9, and then connect the skin to be examined and the vacuum cup 1. and maintain airtightness. Next, the skin adapter 11 is inserted into the cup body 6 so as to be in contact with the O-ring 12. It is preferable to disinfect the skin to be examined with 70% ethanol or disinfectant Hibitane solution beforehand.

Then, the contact flange 9 of the vacuum cup 1 and the skin adapter 11 are gently brought into close contact with the skin to be examined, and the vacuum cup 1 is fixed. For this fixing, the above-mentioned fixing band 15 is used. Alternatively, it can be secured in all four directions with surgical tape.
When obtaining a specimen from an extremity, it is convenient to use the fixation band 15, and for the chest, abdomen, back, etc., it is convenient to use tape for fixation.

After fixing, the vacuum pump 3 is operated to start reducing the pressure inside the vacuum cup 1. The degree of pressure reduction in the test skin area corresponding to the through hole 13 is appropriately controlled by operating the pressure regulating valve 5 and monitored by the vacuum gauge 4. Experimentally, 450mm
Negative pressure on the order of Hg was the most temporally efficient in obtaining epidermal blisters. In addition, at the same time as the start of decompression, a 150W incandescent lamp is turned on at a position 40 to 50 cm away from the test skin to warm the test skin. By heating, the desired epidermal blisters can be obtained more quickly.

After the start of decompression, healthy skin will feel a mild tingling pain in the tested skin area within 30 to 40 minutes. This is the beginning of the formation of epidermal blisters. Usually further
Epidermal blisters are completed in 30 to 40 minutes. During this time, it is desirable to keep the degree of negative suction pressure constant.

Completed epidermal blisters are tense and have slight flushing. Epidermal blisters are excised with a clean scalpel blade while pinching the head with forceps. The obtained epidermal pieces can be used for histological examination, biochemical studies and culture purposes.

[Effects of the invention] As described above, according to the present invention, the configuration includes a monitor section and an adjustment section for the vacuum negative pressure that acts on the skin to be examined through the vacuum cup, which makes it easy to adjust the vacuum negative pressure. In addition to being able to quantitatively monitor the pressure, the pressure can be adjusted arbitrarily, and as a result, epidermal blisters can be produced artificially and efficiently. Furthermore, since the vacuum cup is formed of a translucent container, the state of blister formation can be observed over time from the outside.

[Brief explanation of the drawing]

Fig. 1 is a schematic overall view of an apparatus for producing an artificial epidermal blister according to an embodiment of the present invention, Fig. 2 is an enlarged sectional view of the vacuum cup shown in Fig. 1, and Fig. 3 is a bottom view of the vacuum cup shown in Fig. 2. , FIG. 4 is a perspective view of the vacuum cup with the bottom surface that contacts the skin to be examined facing upward. 1... Vacuum cup, 3... Vacuum pump (suction device),
4... Vacuum gauge (monitor part), 5... Pressure regulating valve (adjusting part), 9... Contact surface flange, 11... Skin surface adapter, 13... Through hole.

Claims (1)

[Claims for Utility Model Registration] Formed by a translucent container,
A vacuum cup whose opening side can be attached to the skin surface, a suction device capable of suctioning the inside of the vacuum cup, a monitor unit for the pressure of the vacuum generated by the suction device, and a device for adjusting the vacuum pressure. An artificial epidermal blister preparation device characterized by having an adjustment section.