JPH0318149B2 - - Google Patents

Info

Publication number: JPH0318149B2
Authority: JP; Japan
Prior art keywords: antigen; red blood; antibody; enzyme; cell
Prior art date: 1982-08-11
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Expired

Application number

JP13945482A

Other languages

English (en)

Japanese (ja)

Other versions

JPS5928661A (ja

Inventor

Fumio Watanabe

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

Toshiba Corp

Original Assignee

Tokyo Shibaura Electric Co Ltd

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1982-08-11

Filing date

1982-08-11

Publication date

1991-03-11

1982-08-11 Application filed by Tokyo Shibaura Electric Co Ltd filed Critical Tokyo Shibaura Electric Co Ltd

1982-08-11 Priority to JP13945482A priority Critical patent/JPS5928661A/ja

1983-08-03 Priority to DE8383107664T priority patent/DE3381055D1/de

1983-08-03 Priority to EP83107664A priority patent/EP0103139B1/de

1984-02-15 Publication of JPS5928661A publication Critical patent/JPS5928661A/ja

1987-06-15 Priority to US07/062,383 priority patent/US4820634A/en

1991-03-11 Publication of JPH0318149B2 publication Critical patent/JPH0318149B2/ja

Status Granted legal-status Critical Current

Links

102000004190 Enzymes Human genes 0.000 claims description 43
108090000790 Enzymes Proteins 0.000 claims description 43
238000003018 immunoassay Methods 0.000 claims description 33
210000003743 erythrocyte Anatomy 0.000 claims description 31
239000000427 antigen Substances 0.000 claims description 26
102000036639 antigens Human genes 0.000 claims description 26
108091007433 antigens Proteins 0.000 claims description 26
210000000170 cell membrane Anatomy 0.000 claims description 21
239000003153 chemical reaction reagent Substances 0.000 claims description 20
239000000758 substrate Substances 0.000 claims description 19
238000006243 chemical reaction Methods 0.000 claims description 14
230000000295 complement effect Effects 0.000 claims description 11
238000006911 enzymatic reaction Methods 0.000 claims description 7
230000009089 cytolysis Effects 0.000 claims 2
230000002934 lysing effect Effects 0.000 claims 1
230000002101 lytic effect Effects 0.000 claims 1
229940088598 enzyme Drugs 0.000 description 38
210000004027 cell Anatomy 0.000 description 28
MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical group OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 10
210000004369 blood Anatomy 0.000 description 10
239000008280 blood Substances 0.000 description 10
102000013529 alpha-Fetoproteins Human genes 0.000 description 8
108010026331 alpha-Fetoproteins Proteins 0.000 description 8
239000007795 chemical reaction product Substances 0.000 description 8
241001465754 Metazoa Species 0.000 description 7
238000000034 method Methods 0.000 description 7
241001494479 Pecora Species 0.000 description 6
238000004458 analytical method Methods 0.000 description 6
230000001461 cytolytic effect Effects 0.000 description 5
239000000644 isotonic solution Substances 0.000 description 5
108010015776 Glucose oxidase Proteins 0.000 description 4
239000004366 Glucose oxidase Substances 0.000 description 4
230000024203 complement activation Effects 0.000 description 4
229940116332 glucose oxidase Drugs 0.000 description 4
235000019420 glucose oxidase Nutrition 0.000 description 4
QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 3
230000000694 effects Effects 0.000 description 3
239000012530 fluid Substances 0.000 description 3
239000001301 oxygen Substances 0.000 description 3
229910052760 oxygen Inorganic materials 0.000 description 3
239000000047 product Substances 0.000 description 3
238000011002 quantification Methods 0.000 description 3
238000003127 radioimmunoassay Methods 0.000 description 3
101000950981 Bacillus subtilis (strain 168) Catabolic NAD-specific glutamate dehydrogenase RocG Proteins 0.000 description 2
102000004641 Fetal Proteins Human genes 0.000 description 2
108010003471 Fetal Proteins Proteins 0.000 description 2
WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
102000016901 Glutamate dehydrogenase Human genes 0.000 description 2
238000011088 calibration curve Methods 0.000 description 2
239000008103 glucose Substances 0.000 description 2
150000002500 ions Chemical group 0.000 description 2
150000002632 lipids Chemical class 0.000 description 2
239000002502 liposome Substances 0.000 description 2
239000012528 membrane Substances 0.000 description 2
238000002360 preparation method Methods 0.000 description 2
210000002966 serum Anatomy 0.000 description 2
241000283707 Capra Species 0.000 description 1
102000004031 Carboxy-Lyases Human genes 0.000 description 1
108090000489 Carboxy-Lyases Proteins 0.000 description 1
241000700199 Cavia porcellus Species 0.000 description 1
108010089254 Cholesterol oxidase Proteins 0.000 description 1
108020005199 Dehydrogenases Proteins 0.000 description 1
108010050375 Glucose 1-Dehydrogenase Proteins 0.000 description 1
102000003855 L-lactate dehydrogenase Human genes 0.000 description 1
108700023483 L-lactate dehydrogenases Proteins 0.000 description 1
241000699670 Mus sp. Species 0.000 description 1
241000283973 Oryctolagus cuniculus Species 0.000 description 1
108090000854 Oxidoreductases Proteins 0.000 description 1
102000004316 Oxidoreductases Human genes 0.000 description 1
LCTONWCANYUPML-UHFFFAOYSA-M Pyruvate Chemical compound CC(=O)C([O-])=O LCTONWCANYUPML-UHFFFAOYSA-M 0.000 description 1
241000700159 Rattus Species 0.000 description 1
108010092464 Urate Oxidase Proteins 0.000 description 1
108010046334 Urease Proteins 0.000 description 1
238000002835 absorbance Methods 0.000 description 1
239000000872 buffer Substances 0.000 description 1
239000007853 buffer solution Substances 0.000 description 1
150000001720 carbohydrates Chemical class 0.000 description 1
235000014633 carbohydrates Nutrition 0.000 description 1
238000007796 conventional method Methods 0.000 description 1
238000001514 detection method Methods 0.000 description 1
238000010586 diagram Methods 0.000 description 1
238000000502 dialysis Methods 0.000 description 1
108010090622 glycerol oxidase Proteins 0.000 description 1
230000003053 immunization Effects 0.000 description 1
238000002649 immunization Methods 0.000 description 1
230000003834 intracellular effect Effects 0.000 description 1
239000007788 liquid Substances 0.000 description 1
238000005259 measurement Methods 0.000 description 1
244000005700 microbiome Species 0.000 description 1
210000003470 mitochondria Anatomy 0.000 description 1
239000000203 mixture Substances 0.000 description 1
230000001590 oxidative effect Effects 0.000 description 1
101710135378 pH 6 antigen Proteins 0.000 description 1
102000004169 proteins and genes Human genes 0.000 description 1
108090000623 proteins and genes Proteins 0.000 description 1
239000000941 radioactive substance Substances 0.000 description 1
150000003839 salts Chemical class 0.000 description 1
230000035945 sensitivity Effects 0.000 description 1
239000000243 solution Substances 0.000 description 1
238000010254 subcutaneous injection Methods 0.000 description 1
239000007929 subcutaneous injection Substances 0.000 description 1
239000000126 substance Substances 0.000 description 1
239000006228 supernatant Substances 0.000 description 1

Classifications

- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/554—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being a biological cell or cell fragment, e.g. bacteria, yeast cells
- G01N33/555—Red blood cell

Landscapes

Health & Medical Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Engineering & Computer Science (AREA)
Immunology (AREA)
Cell Biology (AREA)
Hematology (AREA)
Biomedical Technology (AREA)
Molecular Biology (AREA)
Urology & Nephrology (AREA)
Microbiology (AREA)
Chemical & Material Sciences (AREA)
Biotechnology (AREA)
Mycology (AREA)
Food Science & Technology (AREA)
Medicinal Chemistry (AREA)
Physics & Mathematics (AREA)
Analytical Chemistry (AREA)
Biochemistry (AREA)
General Health & Medical Sciences (AREA)
General Physics & Mathematics (AREA)
Pathology (AREA)
Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

JP13945482A 1982-08-11 1982-08-11 免疫分析用試薬および免疫分析方法 Granted JPS5928661A (ja)

Priority Applications (4)

Application Number	Priority Date	Filing Date	Title
JP13945482A JPS5928661A (ja)	1982-08-11	1982-08-11	免疫分析用試薬および免疫分析方法
DE8383107664T DE3381055D1 (de)	1982-08-11	1983-08-03	Reagenszusammensetzung fuer immuntest und dieselbe verwendender immuntest.
EP83107664A EP0103139B1 (de)	1982-08-11	1983-08-03	Reagenszusammensetzung für Immuntest und dieselbe verwendender Immuntest
US07/062,383 US4820634A (en)	1982-08-11	1987-06-15	Immunoassay method and immunoreactive cell reagent

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
JP13945482A JPS5928661A (ja)	1982-08-11	1982-08-11	免疫分析用試薬および免疫分析方法

Publications (2)

Publication Number	Publication Date
JPS5928661A JPS5928661A (ja)	1984-02-15
JPH0318149B2 true JPH0318149B2 (de)	1991-03-11

Family

ID=15245584

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
JP13945482A Granted JPS5928661A (ja)	1982-08-11	1982-08-11	免疫分析用試薬および免疫分析方法

Country Status (1)

Country	Link
JP (1)	JPS5928661A (de)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
JPH0799374B2 (ja) *	1987-02-06	1995-10-25	株式会社日立製作所	免疫分析用試薬およびこれを用いる免疫分析方法

1982
- 1982-08-11 JP JP13945482A patent/JPS5928661A/ja active Granted

Also Published As

Publication number	Publication date
JPS5928661A (ja)	1984-02-15

Publication	Publication Date	Title
Aizawa et al.	1979	Enzyme immunosenser: Ill. Amperometric determination of human cherienic gonadotropin by membrane-bound antibody
Ivnitski et al.	1996	A one-step, separation-free amperometric enzyme immunosensor
US4820634A (en)	1989-04-11	Immunoassay method and immunoreactive cell reagent
Tang et al.	1991	Electrochemical enzyme immunoassay for phenytoin by flow-injection analysis incorporating a redox coupling agent
US5147781A (en)	1992-09-15	Enzyme electrode and assay for determining LDH5
Solsky et al.	1982	Ion-selective electrodes in biomedical analysis
Wehmeyer et al.	1983	Liquid chromatography with electrochemical detection of phenol and NADH for enzyme immunoassay
JP2638877B2 (ja)	1997-08-06	免疫化学的測定法
Mattiasson et al.	2018	Novel approaches to enzyme-immunoassay
Brown et al.	1991	Potentiometric enzyme channelling immunosensor for proteins
Messina et al.	2005	Continuous-flow/stopped-flow system using an immunobiosensor for quantification of human serum IgG antibodies to Helicobacter pylori
Gotoh et al.	1989	Immuno-FET sensor
JPH0318149B2 (de)	1991-03-11
Monroe	1990	Amperometric immunoassay
EP0125368B1 (de)	1989-06-21	Verfahren zur immunologischen Bestimmung durch Reaktionsgeschwindigkeits-Potentiometrie unter Verwendung von fluoridionenselektiver Elektrode
JP2543630B2 (ja)	1996-10-16	特定タンパク質の糖化割合の測定方法
Monroe	1989	Potentiometric (bioselective electrodes) assay systems: Utility and limitations
Karube et al.	1984	A catalytic immuno-reactor for the amperometric determination of human serum albumin
Aboul-Enein et al.	1999	The construction of an amperometric immunosensor for the thyroid hormone (+)-3, 3′, 5-triiodo-l-thyronine (l-T3)
JPS5984160A (ja)	1984-05-15	免疫分析用試薬および免疫分析方法
Clark Jr et al.	1978	The cholesterol electrode: use of the polarographic oxidase anode with multiple enzymes
EP0399127A1 (de)	1990-11-28	Homogene immunochemische Methode zur Bestimmung von Haptenen mittels ionenselektiver Elektroden
Broyles et al.	1987	Homogeneous and Heterogeneous Enzyme Immunoassays Monitored with Carbon Dioxide Sensing Membrane Electrodes
Heineman et al.	1988	Electrochemical immunosensors
EP0058539A1 (de)	1982-08-25	Enzymatisches Nachweisverfahren und seine Anwendung