JPH03187921A - Production of active substance - Google Patents

Abstract

(57) [Summary] This bulletin contains application data before electronic filing, so abstract data is not recorded.

Description

DETAILED DESCRIPTION OF THE INVENTION The present invention relates to a method for producing an active substance that inhibits various ionic reactions and has physiological effects such as antiviral, anticancer, and immunological effects.

The present inventor has discovered that the active substance obtained by adding ferrous sulfate to a large amount of aqueous hydrochloric acid solution dissolves in water and converts the water into a nonionic reaction system, suppresses various ionic reactions, and can be used in ordinary aqueous systems. It has been found that this method induces a reaction that is significantly different from that caused by an ionic reaction.

It is clear that all normal in-vivo reactions are carried out in the same type of nonionic reaction system formed by the above-mentioned active substances. Therefore, if the above-mentioned substance is introduced into an abnormal living body, the nonionic reaction system within the living body is restored, and the living body can return to normal.

Thus, the above-mentioned active substance obtained by the present invention has physiological effects such as antiseptic action, antiviral action, anticancer action, and immunological action in addition to metal corrosion inhibiting action, salt damage removal action, and soil damage removal action based on ionic reaction inhibition action. It has the following.

The active substance obtained by the present invention is thought to be an iron hydrochloride having properties intermediate between divalent iron and trivalent iron, and is obtained when divalent iron salts are added to a large amount of aqueous hydrochloric acid solution. It is. Specific examples of the method for producing the active substance of the present invention are shown below.

1.0■ ferrous sulfate (FeS04・7H7○)
The mixture was poured into 0 ml of 0.5N aqueous hydrochloric acid solution, stirred and dissolved, and then set up overnight. The resulting insoluble substances are separated, and the liquid f is concentrated under reduced pressure and dried in a desiccator. Add 10 ml of an 80% aqueous solution of isopropyl alcohol to the obtained dry powder, collect the eluted components, concentrate under reduced pressure to remove the solvent, and dry. By repeating the above extraction-concentration-drying operation several times, 0.6 inch crystals are obtained.

In this method, other than ferrous sulfate, divalent iron salts such as ferrous chloride, ferrous nitrate, ferrous phosphate, ferrous formate, and ferrous acetate may be used.

A 5% by weight aqueous solution of the crystals was prepared, and 0.01 ml of it was placed on paper chromatograph paper No. 51A (2αx4
Spot 3an inside from the bottom edge of 0an),
-Upward development is carried out at 20° C. for 15 hours using a mixture of butanol:acetic acid:water=5:1:4 volume ratio as a developing solvent. After the development, the paper was dried and a 1% by weight potassium ferricyanide aqueous solution was sprayed onto the filter paper as a coloring reagent to develop color. It was confirmed that the crystal development position was 1 spot and Rf = 0.07.

3- Then, when a similar paper chromatography test was performed on 1=1 equivalent of ferrous chloride and ferric chloride, the result of development was 2 spots, Rf=0,09
5 (FeC1□) and Rf=0.36 (FeC1a)
It was confirmed that

According to the paper chromatography test described above, the crystals exhibited properties intermediate between those of ferrous chloride and ferric chloride, and are presumed to be a single compound rather than a mixture.

Next, 0.1 g of the crystals was dissolved in distilled water to 100 ml.
Prepare a testable solution. Transfer 2.5 ml of the flask to a 501-volume volumetric flask, add 2.5 ml of 0.1% by weight aqueous orthophenanthroline solution and 2.5 ml of sodium acetate-acetic acid buffer (pH 4,5), and fill the flask up to the mark with distilled water. After standing at room temperature for 30 minutes, absorbance is measured at 510 nm. The divalent iron in the testable solution was determined from the standard curve obtained in the same manner for the ferrous chloride aqueous solution, and was 0.019 g/
It was 100ml.

Next, in the above operation, when the re-test solution is added to the volumetric flask, 1.0 ml of a pre-caulked 10% by weight hydroxylamine hydrochloride aqueous solution is added to reduce trivalent iron to divalent iron. The amount of divalent iron obtained in this case was 0.038g/
It was 100ml. Therefore, the amount of trivalent iron is 0.03
8g/100m1-0.019g/100m1=0.0
19 g/100 ml, suggesting that divalent iron and trivalent iron are present in equivalent amounts in the crystal.

Active substances according to the invention include, for example, sodium chloride, sodium sulfate, ammonium chloride, ammonium sulfate, diatomaceous earth,
It may be supported on inorganic compounds such as bentonite, silica, and alumina, and organic compounds such as vitamins, hormones, proteins, and lipids, and even in such cases, the action of the active substance of the present invention remains unchanged.

Examples of the present invention are shown below.

Example 1 (Corrosion protection of metal) This example shows the corrosion protection effect of the active substance according to the present invention. Corrosion of metals occurs due to the generation of corrosion current between metals of the same type or between metals of different types on the metal surface. Therefore, corrosion protection can be achieved by surface treating metals with a solution containing the active substance of the present invention.

A 0.2 mark X 5 an 1°2 × 10-'g/ml) and glucose (
The mixture was placed in 200 ml of a mixed solution of 10-3 g/l 1 l) and treated at 80°C for 30 minutes.

When the treated iron pieces were subjected to a corrosion test in an HCI air stream, the untreated iron pieces already showed significant corrosion after one hour, but
The treated iron pieces showed no corrosion even after a 6-day corrosion test.

Example 2 (Removal of salt damage) This example shows the effect of the active substance of the present invention on removing salt damage. Electrolyte solutions, especially seawater, pose a great problem to ships and marine/coastal industries due to the metal ions they contain. These obstacles can be eliminated by application of the active substances according to the invention.

When the active substance of the present invention was added to natural seawater at a concentration of 10-12 g/ml, iron powder, manganese powder, and copper powder were added and left to stand, all chlorides were removed from untreated seawater within one day. However, no changes occurred in the treated seawater since 2000.

Example 3 (Improvement of continuous cropping impaired soil) This example shows the effect of the active substance of the present invention on improving continuous cropping impaired soil. When the same crop is continuously cultivated, depending on the crop, pathogenic bacteria may proliferate in the soil, making it almost impossible to harvest. The root cause is ionic reactions between inorganic and organic substances that accumulate in the soil. These obstacles can therefore be eliminated by the introduction of the active substances according to the invention.

The active substance of the present invention containing NaCl as a carrier was diluted with water to a concentration of 10-''g/ml in soil with severe continuous cropping damage accompanied by Fusarium proliferation that occurred in a radish cultivation area (Gifu Prefecture). The diluted solution was applied to the soil to moisten it, and radish was planted in the usual manner.As a result, all the crops in the treated soil grew healthy, and a yield index of 230 was obtained, compared to the yield of 100 in the control plot.

Example 4 (Biological Tissue Preservation) This example shows the biological tissue preservation effect of the active substance of the present invention.

Once a biological tissue is separated from a living individual, biological components such as proteins and carbohydrates immediately begin to decompose because the biological system is destroyed and tissue functions are lost. Since the active substance of the present invention is a basic substance that establishes a biological system,
Even after the living tissue is excised from the living body, tissue disintegration does not occur in the solution containing the active substance of the present invention.

A-tocopherol and Ubiquij non
e (Co-enzymeQ7) 0.1 g of each mixture was added and suspended, and then the above lipid portion was collected with ethanol. The above lipid carrying the active substance of the invention is thus obtained.

Tween-200, 1 is added to this lipid as a surfactant.
g was added and dispersed in water, and sequentially diluted with distilled water to prepare a preparation with a lipid concentration of 2 x 10-''M/L.

Immediately after killing the white rat, the muscle tissue was placed in a bottle, the above-mentioned treatment solution was added, the bottle was tightly capped leaving a partial room air layer, and the bottle was allowed to stand at room temperature. At the same time, as a control, distilled water was added to the muscle tissue and the tissue was sealed tightly and allowed to stand side by side. As a result, the tissue in the control plot collapsed after one week, microorganisms multiplied, and water rapidly dried up. However, the tissue of the samples from the treatment area remained intact, no microbial growth occurred, and the liquid remained clear for more than a month.

Example 5 (Regeneration of plant tissue) This example shows the regeneration effect of the active substance of the present invention on plant tissue.

The active substance of the present invention was prepared in the same manner as in Example 4, and lipid (α-t
ocopherol, Ubiquinone) as a carrier, prepared in a solution with a lipid concentration of 10-7 M/L, and after immersing cut branches of Japanese black pine in the prepared solution for 30 minutes,
The cuttings were placed in pots filled with quartz sand. The control plots all died, but the treated black pines took root.

Example 6 (Abiotic Synthesis of Biological Components) This example shows the effect of the active substance of the present invention on non-biological synthesis of biological components.

The active substances of the invention are involved in the biosynthesis of body proteins in vivo, which is generally known as a genetic phenomenon.

By using the active substance of the present invention, any protein can be synthesized in an abiotic system.

100ml of 1% active substance aqueous solution containing 1■ insulin
The active substance of the present invention was re-extracted from No. 1, washed with ethanol, and then dried and purified. A new aqueous solution containing this crystal as a solute (
2,9x 10-'g/Q) and here 0.1
g of cystine was dissolved, and the solution was subjected to dialysis through a cellulose membrane using distilled water as the external liquid. Ten volumes of the product in the dialysis tube were collected, crystallized, and subjected to various analyses, and it was confirmed that the product was insulin having the same composition as before.

Example 7 (Preservative and antifungal effects) This example shows the antiseptic and antifungal effects of the active substance of the present invention. When the active substance of the present invention comes into contact with microorganisms, it acquires a new protein synthesis function according to the information possessed by the microorganisms. This phenomenon has conventionally been understood as an antigen-antibody reaction.

=9-10- This antibody can be used to preserve foods and prevent mold.

The active substance of the present invention was synthesized using magnesium chloride as a carrier, and a solution with a magnesium chloride concentration of 10-'g/ml was prepared and used as a treatment solution.

The pre-caulked clams and mochi pieces were left standing at 32°C in an open system for 3 days to allow microorganisms to propagate. The resulting microorganisms were placed in a test tube containing 10ml of the above treatment solution, and 0.0% each of starch and peptone were added.
5g and left at 32°C for 5 days. 0.1 ml of the resulting suspension was added to 100 ml of water and this liquid was dipped into fresh clams and rice cakes, sealed and stored at room temperature.

The control plots all showed rot and mold growth, but no microbial growth occurred for more than 3 weeks in the treated samples.

Example 8 (Antiviral action) This example demonstrates the antiviral action of the active substance of the present invention.

When a substance or factor that alters the biological system maintained by the active substance of the present invention invades from outside, the biological function deteriorates and appears as a so-called lesion. Viral infection disorders are caused by the introduction of nucleic acids from the outside and destruction of biological systems. Therefore, by effectively introducing the active substances of the present invention, infectious disorders can be eliminated.

After ingesting TMV into tomato leaves using a pre-caulked tomato as a host plant and causing biological growth, the sap was collected from the tomato leaves immediately before the test and diluted 500 times with water to prepare a test TMV suspension.

After about 1 month, carborundum was applied to leaves of cultivated tobacco plants, and half of the test leaves were treated with TMV suspension diluted 2 times with water as a control, and the other half of the test leaves were treated with TMV suspension. The solution was diluted 2 times with the retesting solution and applied to each cotton pad. The retest solution is 2.9X of the active substance of the present invention.
1% MgC1,-6H2O in 10'g/Q aqueous solution
I used the one that was added so that After the leaves dried after application (approximately 30 minutes later), the remaining carborundum was washed with water, and the plants were cultured in a coitotron at 26°C.

After 3 days of culture, the number of spots that appeared on each test leaf and the inhibition rate of the retested substance were determined, and the results are shown in Table 1.

Table 1 Inhibition of virus infection by active substance solution Example 9 (Anticancer effect) This example shows the anticancer effect of the active substance of the present invention. Malignant tumors are caused when biological systems are severely destroyed by viruses or various carcinogenic substances from the outside, and normal cells migrate to abnormal cells. By introducing the active substance of the present invention here, a biological system is established and the proliferation of abnormal cells can be inhibited.

1 in a 2.9X10-'g/Q aqueous solution of the active substance of the invention.
a-tocopherol and Ubiqu in 0ml
inone (C.

-enzyme Q? ) After adding and suspending 1.0 g of each mixture, the lipid portion was collected with ethanol. The above lipid carrying the active substance of the invention is thus obtained.

This lipid was dispersed in water with 1 g of Tveen-200 added, and diluted with distilled water in order to create a prepared solution. Based on the prepared solution of each concentration, 5 synthetic medium
A culture solution of m 858 was prepared. Cells obtained by trypsium digestion of healthy parts of the human stomach and cancerous tissue in culture media with various lipid concentrations are cultured for 24 hours, and the number of cells is 100 when cultured in a culture solution that does not contain lipids. Figure 1 shows the results of the index. Clear inhibition of abnormal cell growth was observed at 2 x 10-''M, where normal cell growth occurs.

Example 10 (Anticancer effect) This example shows the anticancer effect of the active substance according to the present invention. An active substance complex of the present invention using physiological saline as a carrier was synthesized. The above active substance complex was administered to a terminal stage gastric cancer patient (♀, 46 years old) who had a 5aa x 10an tumor formed in the pyloric region of the stomach, and whose removal surgery was not possible. As a result of applying 70 ml per day, physical strength recovered from the 4th week, and from the 3rd month onward, they were able to go out, increased food intake, and gained weight, and after 6 months, they were able to resume normal daily life. We are now in a position where we can operate.

Since application, liver functions have remained normal.

[Brief explanation of drawings]

FIG. 1 shows the proliferation index in culture medium containing the active substance of the invention. In the figure, the □ line is a normal cell...the line is an abnormal cell

Claims (1)

[Claims] Step 1: Adding salts of divalent iron to a large amount of aqueous hydrochloric acid solution to dissolve them. Step 2: Leaving the solution to remove the resulting insoluble substances. Boiling the solution from which the insoluble substances have been removed to form a solid product. Step 3: Extracting the obtained dry matter with an alcohol-water mixed solvent and concentrating the extract to obtain crystals Step 4: A method for producing an active substance consisting of the above steps 1, 2, 3, and 4.