JPH03225276A - Analyzing disk and analytical method using the analyzing disk - Google Patents

Abstract

PURPOSE:To easily separate and analyze a component having a different specific gravity by forming a hollow part in a disk-like disk body, injecting a sample into the hollow part and then rotating the disk body. CONSTITUTION:Two disk-like bases 2 are connected through ring-like spacers 3 and a hollow part 4 is formed between the bases 2 to constitute the disk body 1. A sample injection port 5 and a spindle hole 6 are formed, the hollow part 4 is charged with a developing solution, a liquid sample is injected from the injection port 5, the spindle hole 6 is set up on a rotation table for an analyzer, and the disk is rotated, so that centrifugal force is applied to the sample, a sample component is separated in the radius direction of the disk to execute the quantitative/quantitative analysis of the sample. Consequently, a component having a different specific gravity is easily separated, and during the rotation of the disk or after stopping the rotation, the component is directly analyzed without applying any treatment to the component.

Description

Detailed Description of the Invention [Field of Industrial Application] The present invention relates to an analytical disk and an analytical method using this disk, and in particular, to an analytical disk that can easily separate components with different specific gravity, and that The present invention relates to an analytical disk that allows component analysis to be performed in the same state after stopping, and an analytical method using the analytical disk.

The present invention is suitably implemented in various analyzes in the medical field, analyzes of substances containing fine powder, and the like.

[Prior Art] Analysis of the type and number (concentration) of component particles contained in liquid samples such as blood and urine is becoming increasingly important from the viewpoint of health management and detection of stage V of epidemics.

Conventionally, these analyzes have generally been performed by observing and analyzing a sample developed on a slide using a microscope.

For example, blood cell components in blood and insect eggs in feces can be analyzed by making a slide for each sample by dropping the sample onto a slide glass, and identifying the target object through mirror observation. There is.

[Problems to be Solved by the Invention] However, in the conventional analysis method using a preparation and an m-microscope as described above, it takes an hour or a lot of time to find the target object because various components are observed in a mixed state. There is a problem with the cost. In addition, since each component is not uniformly distributed on the preparation, there is a problem in that the analysis conditions cannot be kept constant. Furthermore, since all of the above-mentioned work is performed as inspection work, there is a problem in that the inspector feels uncomfortable and unclean.

In addition, in the above-mentioned conventional technology, it is difficult to automate the analysis because various components are mixed and each component is not uniformly distributed, and even if automated, there is a problem that the analysis takes a long time. .

The present invention has been made in view of the above-mentioned problems, and it is possible to easily separate components having different specific gravities, and to analyze the components during rotation or after the rotation has stopped. Analyzes can be performed quickly under the same analytical conditions. The purpose of the present invention is to provide an analysis disk suitable for automation and an analysis method using the disk.

[L stage for solving the problem] In order to achieve the purpose described in [L stage], the analytical disk of the present invention has
The configuration includes a hollow part for separating and developing an analytical sample, and a sample injection port for injecting the analytical sample into the hollow part, and preferably the 11 hollow parts are formed by two plates, at least one of which is transparent. The structure is such that the disks and ζ plates are held in a straight line, and more preferably the hollow portion is divided into a plurality of inspection sections by a separating wall.

In addition, an analysis method using the analysis disk of the present invention is such that an analysis sample is injected into the above-mentioned analysis disk, and the disk is rotated to separate and analyze components in the sample. The analysis is carried out by optical analysis means, and more preferably the analysis is carried out while the disk is rotating or after it has stopped.

[Function] The analytical disk according to the present invention has a hollow part inside the disk-shaped disk body, and is used by injecting an analytical sample into this hollow part and then rotating it. The components can be easily separated, and the components can be analyzed during rotation or after the rotation has stopped.

Furthermore, since the analysis method using the analytical disk according to the present invention uses the above-mentioned analytical disk, only one separation operation is required, and the analysis is performed after separating and fractionating the sample under certain conditions. This enables rapid analysis. Furthermore, since the sample can be analyzed in its original state without being removed from the disk either while the disk is rotating or after the rotation has stopped, quick JS analysis is possible. In addition, due to the ease of separation operation and rapid analysis,
This is an analysis method suitable for automation.

[Examples] Below, an analytical disk and an analytical method using the analytical disk according to the present invention will be specifically described based on examples.

First, an example of one analysis disk will be described.

FIGS. 1(a) and 1(b) are a plan view and a sectional view showing an analysis disk according to an embodiment of the present invention.

In the figure, l represents the disk body, which has a disk-like shape. This disk body 1 has a structure in which two disk-shaped disk substrates 2 are joined by a ring-shaped spacer 3, and a hollow portion 4 is formed inside thereof.

2 is the disk substrate, which is made of polycarbonate.

It is formed from plastics such as polystyrene and polymethyl methacrylate, transparent materials such as glass, or non-transparent materials such as ceramics and metals. When performing analysis using optical analysis means, it is necessary to use a transparent material for at least one of the disk substrates.For example, when performing analysis using reflected light, one disk substrate must be transparent; When performing analysis using transmitted light, both disk substrates must be transparent. Further, for example, if changes in electrical parameters such as impedance are used as an analysis means, both disk substrates may be formed of a non-transparent material such as ceramic or metal coated with an insulator.

3 is a spacer, which has a link-like shape. Spacer 3 consists of two disk substrates 2.

It is held parallel by a certain distance.

The two disk substrates 2 and the spacer 3 are closely attached to each other by an adhesive or the like.

The thickness of the hollow portion 4 formed by joining the two disk substrates 2 via the spacer 3 is not particularly limited, and is appropriately selected depending on the type of sample to be analyzed, the viscosity of the developing solution, and the like. Further, the hollow portion 4 may be divided into a plurality of inspection sections having an arbitrary number and shape by a separation wall (not shown).

There is no particular limitation on the method of division, but it is preferable to divide the hollow part 4 into a plurality of inspection sections in this way by dividing it in the circumferential direction using a separation wall 7 extending in the radial direction, as shown in FIG. , it is possible to perform multiple analyzes at the same time.

Reference numeral 5 denotes a sample injection port, which is provided for injecting the analytical sample 1 into the hollow portion 4. The size and shape of the sample injection port 5 are not particularly limited.It is preferable to provide the sample inlet 5 at the center of the disk as shown in the figure. It is also possible to prevent the sample from scattering to the outside by providing a lid on the injection port 5 or by forming the sample injection port 5 from a material such as rubber and injecting the sample using a syringe.

In addition, when the hollow part 4 is divided into a plurality of inspection sections,
It is preferable to provide a sample injection port 5 for each inspection section (
(See Figure 2).

Reference numeral 6 has a shaft hole, which is provided for setting the analytical disk on a rotating shaft of a rotary table or the like of an analyzer when rotating the analytical disk. Note that the shaft hole 6 may not be provided depending on the configuration of the rotating machine (separator) of the analyzer.

The uninvented analysis disk substrate is not limited to the above embodiments, and can be modified as appropriate.

For example, the shape of the analysis disk is not particularly limited as long as it has a hollow part, and is not limited to the above-mentioned disk shape, and the outer periphery of the analysis disk can be used to easily clean the inside of the hollow part 4. An opening/closing hole may be provided for the purpose of opening and closing.

Furthermore, the analytical disk having the hollow portion 4 may be molded in one piece.

Next, an analysis method using the analysis disk of the present invention will be explained.

In the analysis method of the present invention, the analysis sample includes blood,
Examples include liquid samples such as urine and feces, liquid samples containing or dispersing inorganic microparticles, samples containing impurities, or containing components with different specific gravity. It can be applied to any sample.Analytical samples are subject to various adjustments depending on the purpose of the analysis and the characteristics of the analytical means.For example, liquids that do not affect the analyte (such as alcohol, The specific gravity is adjusted by mixing water, etc.), the viscosity is adjusted by increasing the viscosity by adding ethylene glycol, polyvinyl alcohol, etc., or the degree of a is adjusted so that particles do not overlap. .

Next, the hollow portion 4 of the disk body 1 is filled with a developing solution.

The developing solution alters the quality of the disk plate and sample.

It is necessary to use something that does not cause deformation. - Use water, liquid solution, etc. inside the shell. Further, it is more effective for fractionation to use a mixed liquid (for example, water and polyethylene glycol) that generates a density gradient in the radial direction due to centrifugal force.

In addition, it is also possible to use a solution obtained by diluting the sample without using any developing solution.For example, in the case of blood.

A solution diluted 100 to 5000 times with physiological saline or the like can be used.

Next, a sample is injected into the sample injection port of the analysis disk. The sample used is usually a liquid sample that has been subjected to the various adjustments described above.

After the sample is injected, the disk is rotated to apply centrifugal force to the sample, and the components in the sample are separated and fractionated in the radial direction of the disk due to the difference in density of the sample.

Note that the rotational speed of the disk is appropriately selected so as to provide a condition in which the sample can be appropriately separated into one fraction by centrifugal force. It varies depending on the sample, but usually 500 to 3000 (rpm
) is rotated.

Quantitative and/or qualitative analysis of the sample is performed after the sample has been completely collected.

As the analysis means, various optical analysis means are used, for example, which combine a light source such as a laser, an LED, or a halogen lamp, and a light receiving system such as a photodetector CCD (line sensor).

In this case, whether to use reflected light, transmitted light, or absorbed light is determined depending on one analysis sample.

Note that the optical analysis means here also includes optical image analysis. For example, an optical image is converted into an electronic image, and an image of particles that matches the shape of a specific particle stored in advance is extracted from the electronic image. The analysis is done by doing this.

Further, the analysis may be performed by visual observation or microscopic observation, or by using electrical analysis means such as the impedance described above.

Analysis can be performed either while the disk is rotating or after it has stopped rotating. Furthermore, in either case, analysis can be performed without removing the sample from the disk.

When the analysis is performed during rotation, an optical analysis means is mainly used. For example, as shown in FIG. 2, an optical analysis means consisting of a laser light source 10 and a photodetector 11 is used. Because laser light can be focused.

It is possible to collect information on the order of 1 μm.

The analysis is performed at a radial position where the object is divided. In this case, as shown in FIG. 2, if the laser light source lO and photodetector 11 are made reciprocating in the radial direction A of the disk, multiple components fractionated in the radial direction of the disk can be analyzed simultaneously. Ru. Note that the number of times the disk surface is analyzed is not limited to one time, but may be performed multiple times. Performing the analysis multiple times in this manner is useful for obtaining data with fewer errors or for discovering abnormal values.

Furthermore, when performing analysis using a disk whose hollow part is divided in the circumferential direction by a separation wall, the standard sample may be injected into one of the divided sections. You can perform more useful analysis in terms of data reliability, etc. by referring to .

When analysis is performed after rotation has stopped, visual observation,
This can be done by m* mirror observation or any other analytical means.

After the rotation of the disk has stopped, the separated and fractionated samples tend to be homogenized by diffusion, etc.

It is preferable to conduct the analysis within a certain period of time after the rotation has stopped.
Note that by making the thickness of the hollow part thin or by controlling the viscosity of the developing solution, it is possible to maintain the separated state of the sample for a long time even after the rotation has stopped.

According to the analysis method of the present invention, for example, qualitative and quantitative analysis of blood cell components in blood can be easily performed. Furthermore, when used in the analysis of samples containing minute inorganic particles, the particle size and number of particles can be easily measured, and therefore a call counter can be used.
It can also be used as a substitute for.

[Effects of the Invention] As explained above, according to the analytical disk of the present invention, components having different specific gravity can be easily separated, and the components can be analyzed during rotation or after the rotation has stopped. Ru.

Therefore, it provides an analysis method that can be analyzed quickly under certain analysis conditions and is suitable for automation.

[Brief explanation of drawings]

FIGS. 1(a) and 1(b) are a plan view and a sectional view showing an analysis disk according to an embodiment of the present invention. FIG. 2 is a partially cutaway plan view of an analytical disk having a plurality of test sections, and FIG. 3 is a schematic diagram showing a specific example of the analytical method using the analytical disk of the present invention. l・Disk body 2 Disk substrate 3, spacer 4 Hollow part 5 Sample injection port

Claims (6)

[Claims] (1) An analysis disk characterized by having a hollow part for separating and developing an analysis sample, and a sample injection port for injecting the analysis sample into the hollow part. (2) The analytical disk according to claim 1, wherein the hollow portion is formed by holding two disk substrates, at least one of which is transparent, in parallel. (3) The analytical disk according to claim 1 or 2, wherein the hollow portion is divided into a plurality of inspection sections by a separation wall. (4) An analysis method comprising injecting an analysis sample into the analysis disk according to any one of claims 1 to 3, and rotating the disk to separate and analyze components in the sample. (5) The analysis method according to claim 4, wherein the analysis is performed by optical analysis means. (6) The analysis method according to claim 4 or 5, wherein the analysis is performed while the disk is rotating or after the disk is stopped.