JPH03227995A - Production of fructo-oligosaccharide - Google Patents

Abstract

PURPOSE:To obtain the subject hardly digestible compound, having low cariogenicity and physiological action such as improvement in intestinal bacterial floras and lowering of cholesterol in blood and useful in the field of food, pharmaceutical industries, etc., by pressing the juice from yacon (Polymnia sonchifolia). CONSTITUTION:The juice is pressed from yacon or extraction thereof with a solvent (preferably water or a water-soluble organic solvent) is carried out to afford the objective compound.

Description

[Detailed Description of the Invention] [Industrial Application Field] The present invention relates to a method for producing fructooligosaccharides, and more particularly, to a method for producing fructooligosaccharides characterized by squeezing juice from yacon tubers or extracting with a solvent. .

[Prior art and problems to be solved by the invention] Fructooligosaccharides have conventionally been produced by flowing a sucrose solution through a column of immobilized bacterial cells or immobilized enzymes that have fructose transfer activity, and are not directly extracted from natural products. However, the manufacturing method has not yet been established.

Therefore, the present inventors searched for various natural products and found that yacon tuberous root contained a large amount of Flag I oligosaccharide, and based on this knowledge, completed the present invention.

[Means to solve the problem]

The present invention provides a method for producing fructooligosaccharide characterized by squeezing yacon and a method for producing fructooligosaccharide characterized by extracting yacon with a solvent.

Yacon (Yacon) belongs to the Asteraceae family and is a root vegetable native to the Andean highlands.
Yacδn) + Jiguima + Jiguimilla.

Arboloco (Arboloco) Alicoma (A
ricuma).

It is also called Aricoma, and its scientific name is Polymnia 5onchifolia or P
It is olymnia edulis.

To produce fructooligosaccharides from yacon, especially yacon tubers, juice extraction or solvent extraction is performed as described above.

Since yacon contains about 86% water, juice can be easily obtained by squeezing it with an appropriate juicer.

Moreover, an extract containing fructooligosaccharides can be obtained by extraction using water (including hot water) or a water-soluble organic solvent. Here, as the water-soluble organic solvent, in addition to ethanol, methanol, acetone, acetonitrile, etc. can also be used, but ethanol is particularly suitable. Ethanol is preferably used as an aqueous solution, usually 20-90%
An aqueous solution of about 100% is used. Furthermore, when squeezing or extracting yacon, enzymes may be deactivated by heat treatment in advance, and the juice or extract can be used as is as a crude fructooligosaccharide solution, but it can also be used by activated carbon column chromatography, etc. It can also be used after being purified. If further purification is desired, purification methods commonly used for crude fructooligosaccharide solutions may be applied.

FIG. 1 shows an example of a method for extracting yacon tuberous roots using a water-soluble organic solvent.

After adjusting the PL+ of the yacon juice or extract to near neutrality, it is concentrated by vacuum concentration, etc., dissolved in distilled water, washed with an organic solvent such as chloroform, and separated from the organic solvent layer to form an aqueous layer. After concentrating again, ethanol is added and stirred, followed by solid-liquid separation means such as filtration and centrifugation to obtain a solid content, which is dissolved in distilled water and purified by chromatography such as activated carbon column chromatography. Plaque 1 purified by vacuum concentration if necessary
- Oligosaccharides are obtained.

The fructooligo* store contained in yacon is G when glucose is expressed as G, fructose is expressed as F, and the number of bonds is expressed as n.
F, and CF2 to CF exist, with CF being the most common. Refined trisaccharides and kesdose (CF) obtained from yacon
2) Comparison of the NMR spectra of the standard product 13(,-NMR) revealed the results shown in Figure 2, which revealed that this fructooligosaccharide has inulin-type β-1,2 bonds. became.

〔Example〕

Next, the present invention will be explained using examples.

Example 1 Fructooligosaccharides were produced from yacon tuberous roots by the process shown in FIG. That is, 300g of yacon tuber
Extract by adding 80% ethanol 500 to
A soluble fraction containing fructooligosaccharide was obtained by the method shown in the figure. The carbohydrate contents of both portions are shown in Table 1.

Table 1 1 把 1 分 一 くら m/- ■ 1- Fructose 350.1 + 42.
0 glucose 158.3 +28.6
Sucrose 74.5 + 19.0
CF2 60.1 +12.6GF,
47.4 + 8.2CF4 33.6 +
9.3 CF520.6 + 5.2 CF615.8 + 4.0 CF712.7 + 4.0 CF89.6 + 7.2 CF9 6.6 +2.3 Inulin in the remaining fraction was 13.5 mg/ g dry matter, and no starch was detected.

[Effects of the Invention] According to the present invention, fructooligosaccharides can be obtained from yacon through simple operations. This fructo-oligosaccharide not only has low caries properties and is indigestible, but also has physiological effects such as improving intestinal bacterial flora and lowering blood cholesterol.

Moreover, since yacon can be produced in large quantities,
As a fructooligosaccharide produced and purified from natural products, it is expected to be used in fields such as the food industry and the pharmaceutical industry.

[Brief explanation of drawings]

FIG. 1 shows an example of a method for extracting yacon tuberous roots using a water-soluble organic solvent. No. 27a is purified tri-M obtained from yacon
Figure 2 shows the I3CNMR spectrum of the Kesdos (CF2) sample.

Claims (3)

[Claims] (1) A method for producing fructooligosaccharides, which comprises squeezing yacon. (2) A method for producing fructooligosaccharides, which comprises extracting yacon with a solvent. (3) The method according to claim 2, wherein the solvent is water and/or a water-soluble organic solvent.