JPH0331753A - Method for measuring concentration of chlorine ion in biofluid - Google Patents
Method for measuring concentration of chlorine ion in biofluidInfo
- Publication number
- JPH0331753A JPH0331753A JP16525889A JP16525889A JPH0331753A JP H0331753 A JPH0331753 A JP H0331753A JP 16525889 A JP16525889 A JP 16525889A JP 16525889 A JP16525889 A JP 16525889A JP H0331753 A JPH0331753 A JP H0331753A
- Authority
- JP
- Japan
- Prior art keywords
- biological fluid
- measuring
- concentration
- electrode
- biofluid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- VEXZGXHMUGYJMC-UHFFFAOYSA-M Chloride anion Chemical compound [Cl-] VEXZGXHMUGYJMC-UHFFFAOYSA-M 0.000 title claims description 24
- 238000000034 method Methods 0.000 title claims description 21
- 239000000460 chlorine Substances 0.000 claims abstract description 12
- 229910052801 chlorine Inorganic materials 0.000 claims abstract description 12
- -1 chlorine ions Chemical class 0.000 claims abstract description 10
- 229910021607 Silver chloride Inorganic materials 0.000 claims abstract description 9
- HKZLPVFGJNLROG-UHFFFAOYSA-M silver monochloride Chemical compound [Cl-].[Ag+] HKZLPVFGJNLROG-UHFFFAOYSA-M 0.000 claims abstract description 9
- 230000003647 oxidation Effects 0.000 claims abstract description 7
- 238000007254 oxidation reaction Methods 0.000 claims abstract description 7
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 7
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 7
- 229910052709 silver Inorganic materials 0.000 claims abstract description 7
- 239000004332 silver Substances 0.000 claims abstract description 7
- 229910001220 stainless steel Inorganic materials 0.000 claims abstract description 3
- 239000010935 stainless steel Substances 0.000 claims abstract description 3
- 239000013060 biological fluid Substances 0.000 claims description 40
- 210000004369 blood Anatomy 0.000 claims description 10
- 239000008280 blood Substances 0.000 claims description 10
- 229920005597 polymer membrane Polymers 0.000 claims description 8
- 210000002966 serum Anatomy 0.000 claims description 7
- 210000002381 plasma Anatomy 0.000 claims description 6
- 239000000126 substance Substances 0.000 claims description 6
- 239000012530 fluid Substances 0.000 claims description 5
- 150000002500 ions Chemical class 0.000 claims description 5
- 229920000515 polycarbonate Polymers 0.000 claims description 5
- 239000004417 polycarbonate Substances 0.000 claims description 5
- 210000003296 saliva Anatomy 0.000 claims description 4
- 238000007865 diluting Methods 0.000 claims description 3
- 239000012153 distilled water Substances 0.000 claims description 3
- 239000007788 liquid Substances 0.000 claims description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 3
- ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 claims description 2
- 239000007853 buffer solution Substances 0.000 claims description 2
- 229920002301 cellulose acetate Polymers 0.000 claims description 2
- 210000002751 lymph Anatomy 0.000 claims description 2
- 238000005259 measurement Methods 0.000 abstract description 11
- 238000011088 calibration curve Methods 0.000 abstract description 6
- BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 abstract description 5
- 239000011148 porous material Substances 0.000 abstract description 5
- 239000000463 material Substances 0.000 abstract description 3
- 239000011248 coating agent Substances 0.000 abstract 1
- 238000000576 coating method Methods 0.000 abstract 1
- 229920000642 polymer Polymers 0.000 abstract 1
- 239000012528 membrane Substances 0.000 description 5
- 238000006243 chemical reaction Methods 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 238000000691 measurement method Methods 0.000 description 3
- 241000251539 Vertebrata <Metazoa> Species 0.000 description 2
- 230000000052 comparative effect Effects 0.000 description 2
- 239000003792 electrolyte Substances 0.000 description 2
- 102000001554 Hemoglobins Human genes 0.000 description 1
- 108010054147 Hemoglobins Proteins 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 238000009614 chemical analysis method Methods 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000005443 coulometric titration Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 238000000835 electrochemical detection Methods 0.000 description 1
- 210000004880 lymph fluid Anatomy 0.000 description 1
- 229920006254 polymer film Polymers 0.000 description 1
- 229910001414 potassium ion Inorganic materials 0.000 description 1
- 229910001415 sodium ion Inorganic materials 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
Landscapes
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
Description
【発明の詳細な説明】
［産業上の利用分野〕
本発明は、電気化学的な検出法で、生体液中に含有され
る塩素イオンの濃度を定量的に測定する方法に関する。DETAILED DESCRIPTION OF THE INVENTION [Industrial Application Field] The present invention relates to a method for quantitatively measuring the concentration of chloride ions contained in a biological fluid using an electrochemical detection method.
［従来の技術〕
従来、生体液中に含有される塩素イオンの定量としては
、シャレス−シャレス法 （Ｓ ｃ ｈ ａＩｅｓ
５ｃｈａｌｅｓ）−電量滴定法、イオンＺ Ｎ法、な
ど種々のものがりる。[Prior Art] Conventionally, the Schales-Chares method has been used to quantify chlorine ions contained in biological fluids.
5chales) - There are various methods such as coulometric titration method and ion ZN method.
しかしながら、いずれも生体液をそのまま使用して塩素
イオンの定置を行うものではなく採取後ただちに生体液
を処理する必要があり、さらに薬物の干渉による誤差が
発生ずる等の問題があり、試料調製の操作が煩雑でその
上化学分析技術の習熟が必要とされる。 特に生体液が
血液の場合は血清を分離しなければならず−このような
余計な操作を必要とし測定法としては複雑にならざるを
得ない欠点がある。However, these methods do not use biofluids as they are to immobilize chloride ions, and the biofluids must be treated immediately after collection. Furthermore, there are problems such as errors caused by drug interference, and sample preparation is difficult. The operation is complicated and requires proficiency in chemical analysis techniques. In particular, when the biological fluid is blood, serum must be separated - such an extra operation is required, and the measurement method is inevitably complicated.
し・発明が解決しようとする問題点］
前記した様に、従来の生体液の塩素イオンの定置は、試
料の調製を含め定量操作が煩雑なため、迅速性かつ正確
を要求される分析には適さない欠点があるばかりか、繰
り返し分析精度の良い再現性を得るのがむずかしいとい
う問題点を含んでいるので、本発明はこれらの欠点なら
びに問題点を解決したものである。[Problems to be Solved by the Invention] As mentioned above, the conventional emplacement of chloride ions in biological fluids requires complicated quantitative operations including sample preparation, so it is not suitable for analyzes that require speed and accuracy. The present invention solves these drawbacks and problems, since it not only has disadvantages that it is not suitable, but also that it is difficult to obtain good reproducibility with repeated analysis accuracy.
［発明が解決しようとする課題］
本発明は、前記した従来の測定法の欠点ならびに問題点
を改善し、被検生体液中の塩素イオンの濃度を短時間に
、しかも操作は容易で、要求される繰り返し分析精度で
定量することができる、生体液に含有される塩素イオン
のＪ度を測定する方゛法を提供することを、その目的と
する６［課題間を解決するための手段］
本発明は、
「１． 同一槽内にある生体液中に、銀で構成１７た
作用電極およびステンレスで構成した対極と、銀／塩化
銀で構成した参照電極とからなる電極を塩素イオンは通
過することができるが、生体液中に含まれる蛋白質など
の共存物質を通過させない、微細多孔を有する高分子膜
状物で覆って構成した測定電極を設置し、作用電極の電
位レベルを対極に対して −０，６から＋〇、６Ｖ
ｖｓ、Ａｇ／Ａｇ／ＡｇＣｌに次いで＋０，６から一〇
、６Ｖ Ｖｓ、 Ａ ｇ／Ａ ｇ Ｃｊの範囲でサイク
リックに変化させ、その電圧変化に応じて発生する！？
ｉ変化を連続して得られる酸化波及び還元波の波高値と
して測定して、予め作成した検量線から被検生体液中に
含有されるＣＩイオンの濃度を測定する方法。[Problems to be Solved by the Invention] The present invention improves the drawbacks and problems of the conventional measurement methods described above, and enables the concentration of chloride ions in the biological fluid to be measured in a short time, with easy operation, and to meet the requirements. The objective is to provide a method for measuring the degree of J of chloride ions contained in biological fluids, which can be quantified with repeatable analytical accuracy. 6 [Means for solving the problems] The present invention is based on the following points: 1. Chlorine ions pass through an electrode consisting of a working electrode made of silver, a counter electrode made of stainless steel, and a reference electrode made of silver/silver chloride in a biological fluid in the same tank. However, by installing a measuring electrode covered with a polymer membrane with microporous that does not allow coexisting substances such as proteins contained in biological fluids to pass through, the potential level of the working electrode is set relative to the counter electrode. -0,6 to +〇,6V
Vs, Ag/Ag/AgCl, then cyclically changed in the range of +0, 6 to 10, 6V Vs, A g/A g Cj, and generated according to the voltage change! ?
A method of measuring the concentration of CI ions contained in a sample biological fluid from a calibration curve prepared in advance by measuring i changes as the peak values of continuously obtained oxidation waves and reduction waves.
２、生体液が、血液、血清、血漿、リンパ液、髄液、唾
液から選んだ液である請求項Ｉに記載された、被検生体
液中に含有される塩素イオンの濃度を測定する方法。2. The method for measuring the concentration of chloride ions contained in a test biological fluid according to claim I, wherein the biological fluid is a fluid selected from blood, serum, plasma, lymph, spinal fluid, and saliva.
３ 生体液が、生体液を緩衝液又は蒸溜水で稀釈くしな
液である請求項１に記載された、被検生体液中に含有さ
れる塩素イオンの濃度を測定する方法。3. The method for measuring the concentration of chloride ions contained in a test biological fluid according to claim 1, wherein the biological fluid is a comb solution obtained by diluting the biological fluid with a buffer solution or distilled water.
４、微細多孔を有する高分子膜状物がポリカーボネート
、セルロースアセテート、から選んだ膜状物である請求
項１ないし３のいずれか１項に記載された、被検生体液
中に含有される塩素イオンの濃度を測定する方法。4. Chlorine contained in the test biological fluid according to any one of claims 1 to 3, wherein the microporous polymer membrane is selected from polycarbonate and cellulose acetate. A method of measuring the concentration of ions.
５、＠細条化を有する高分子膜状物が５〜７ミクロンの
膜厚である、請求項１ないし４のいずれか１項に記載さ
れた、被検生体２α中に含有される塩素イオンの濃度を
測定する方法。5. Chlorine ions contained in the test organism 2α according to any one of claims 1 to 4, wherein the polymer film having striations has a film thickness of 5 to 7 microns. How to measure the concentration of.
６、 高分子膜状物に配設したｍ細条化の直径が０．０
３ミクロン以下である、請求項１ない！−５のいずれか
１項に記載された。被検生体液中に含有される塩素イオ
ンの濃度を測定する方法、Ｊである。6. The diameter of the m strips arranged on the polymer membrane is 0.0.
There is no claim 1, which is 3 microns or less! -5. J is a method for measuring the concentration of chloride ions contained in a sample biological fluid.
生体液には、種々の電解質が含有されているがその中で
もナトリウムイオン、カリウムイオン、塩素イオンが重
要な電解質でありそれらのイオンの量を測定することに
より生体の状態を知ることが出来る。Biological fluids contain various electrolytes, among which sodium ions, potassium ions, and chloride ions are important electrolytes, and the state of the living body can be determined by measuring the amounts of these ions.
本発明の第１の特徴は採取した′＆まの生体液で面接合
まれる塩素イオンの：ａ反をＰ１定できることである。The first feature of the present invention is that it is possible to determine the :a ratio P1 of chloride ions that are brought into contact with each other in the collected biological fluid.
生体液としては、血液、血清、血漿、すンバ湾、Ｎ液、
唾液など種々のものがあるが、壺も代表的なものは血液
であるのでこの血液について説明すると採取したそのま
までも、血清、血漿等を適宜分離してから測定すること
もできるし、分離した血清、血漿等について塩素イオン
の濃度を測定することもできるａｔた、［を液又は蒸溜
水で稀釈して測定することもできる。この様な測定法は
血液に限らずリンパ液、ＭＨ１唾液などの全ての生体液
について同様に行うことができる。Biological fluids include blood, serum, plasma, Sumba Bay, N fluid,
There are various things such as saliva, but blood is the most common type of blood in the jar.To explain about blood, it can be measured as it is, or after separating serum, plasma, etc. as appropriate, or it can be measured after separating serum, plasma, etc. The concentration of chloride ions can be measured in plasma, etc., and can also be measured by diluting it with liquid or distilled water. Such a measurement method can be performed not only for blood but also for all biological fluids such as lymph fluid and MH1 saliva.
本発明は全てのを椎動物、無節を椎動物の生体液ばかり
でなく、植物の生体液についても含有される塩素イオン
の濃度を測定できるのである。The present invention can measure the concentration of chloride ions contained not only in the biological fluids of all vertebrates and non-vertebrates, but also in the biological fluids of plants.
本発明の第２の特徴は測定電極を生体液中に含まれる蛋
白質などの共存物質を通過させないｍｓ多孔を有する高
分子膜状物で覆って構成したことである。高分子膜状物
としては、ポリカーボネート、セルロースアセテ−１−
等から得た膜状物が使用される。Ｓ厚は５〜７μｍが好
適であり、これより厚くなると塩素イオンの透過が遅く
なるので、測定時間が長くなる欠点が生ずる。微細孔は
直径が０．０３μｍ以下である必要がある。孔がこれよ
り大きいと生体液中に含まれる蛋白質などの共存物質が
通過してしまうので測定結果にＳ影響を及ぼすからであ
る。この様な特種の膜で測定電極を覆うので採取したま
まの生体液で面接合まれる塩素イオンの濃度を測定でき
るのである。The second feature of the present invention is that the measurement electrode is covered with a polymeric membrane having ms pores that do not allow coexisting substances such as proteins contained in biological fluids to pass through. Polycarbonate, cellulose acetate-1-
A film-like material obtained from etc. is used. The S thickness is preferably 5 to 7 μm; if it is thicker than this, the permeation of chlorine ions becomes slow, resulting in a disadvantage that the measurement time becomes longer. The micropores need to have a diameter of 0.03 μm or less. This is because if the pores are larger than this, coexisting substances such as proteins contained in the biological fluid will pass through, which will affect the measurement results. By covering the measurement electrode with such a special membrane, it is possible to measure the concentration of chloride ions that are present in the biological fluid as it is collected.
このことを代表的な生体液である血液について詳しく説
明すると高分子膜状物のｒＲ細孔の直径が０゜０３μｍ
これより大きいと血液中に含まれる蛋白質、ヘモグロビ
ンなどの共存物質を通過してしまうのである。To explain this in detail regarding blood, which is a typical biological fluid, the diameter of the rR pores of the polymer membrane is 0°03 μm.
If it is larger than this, coexisting substances such as proteins and hemoglobin contained in the blood will pass through.
本発明の第３の特徴は測定電極の作用電極の電位レベル
を対極に対し −０，６から＋０．６Ｖ ＶＳ、ＡＱ／
ＡｇＣ１に次いで＋０．６から一〇、６Ｖ ｖｓ、 Ａ
ｏ／Ａ ｇ ／ＡｇＣｌの範囲でサイクリックに変化
させ、その電圧変化に応じて発生する電流変化を連続し
て得られる酸化波及び還元波の波高値として測定して塩
素イオン濃度を測定することである。The third feature of the present invention is to change the potential level of the working electrode of the measuring electrode from -0.6 to +0.6 V VS, AQ/
AgC1 followed by +0.6 to 10, 6V vs. A
To measure the chloride ion concentration by cyclically changing the voltage in the range o/Ag/AgCl and measuring the current change that occurs in response to the voltage change as the peak value of the continuously obtained oxidation wave and reduction wave. It is.
［作 用］
本発明は、前述のように特種な、微細多孔を有する高分
子膜状物で測定電極を覆い生体液中に含まれる蛋白質な
どの共存物質の通過を防止するので採取したままの生体
液で面接合まれる塩素イオンの濃度を測定できる格別の
作用が奏される。[Function] As mentioned above, the present invention covers the measurement electrode with a special polymeric membrane having micropores to prevent the passage of coexisting substances such as proteins contained in biological fluids. It has a special ability to measure the concentration of chloride ions that are present in biological fluids.
また、本発明においてはまず測定電極の作用電極の電位
レベルを対極に対し−０，６から＋〇、６Ｖ ｖｓ。In the present invention, first, the potential level of the working electrode of the measurement electrode is set from -0.6 to +0.6 V vs. the counter electrode.
１７／Ａｇ／ＡｇＣｌ！に変化させてＡｇ＋ＣＩ−Ａｇ
Ｃｌモｅ−の反応による酸化電流を測定しついで、＋０
．６から−０，６Ｖ ＶＳ、／ｇ／ＡｇＣｌにサイクリ
ックに変化させてＡｇＣＩ ＋ｅ−−Ａｇ−）−ＣＩ−
の反応による還元電流を測定することができる。17/Ag/AgCl! Change it to Ag+CI-Ag
After measuring the oxidation current due to the reaction of Clmoe-, +0
．． AgCI +e--Ag-)-CI- by cyclically changing from 6 to -0,6V VS, /g/AgCl
The reduction current due to the reaction can be measured.
サイクリックに変化させることにより、Ａ ａｔ極に生
成したＡｇＣ１を溶解して自動的に電極を洗浄すること
ができる。By cyclically changing it, AgC1 generated in the A at electrode can be dissolved and the electrode can be automatically cleaned.
［実施Ｓ］
以下、本発明の一実施例を第１図ないし第２図に従って
説明する。[Embodiment S] An embodiment of the present invention will be described below with reference to FIGS. 1 and 2.
第１図に、本発明を実施例について具体的に説明する。In FIG. 1, the present invention will be specifically explained with reference to an embodiment.
第１図は本発明の実施例のボルタモダラムを示＋、、作
用電極１として銀電極を用い、被検液として株式会社常
光製の血清を使用したボルタモダラムである。FIG. 1 shows a volta modalum according to an embodiment of the present invention, in which a silver electrode was used as the working electrode 1 and serum manufactured by Joko Co., Ltd. was used as the test liquid.
第１図の縦軸は波高値を示しそして横軸はＶｖｓ。The vertical axis in FIG. 1 shows the peak value, and the horizontal axis shows Vvs.
Ａｇ／ＡｇＣ１を示す。Indicates Ag/AgC1.
測定電極は膜厚平均５μｍで直径はぼ０．０３μｍの微
細多孔を有するポリカーボネート膜で包まれている。The measuring electrode is wrapped in a polycarbonate membrane having an average thickness of 5 μm and micropores of approximately 0.03 μm in diameter.
第１図の曲線から明らかなように、作用ｔ％の電位を対
極に対して一〇、６から＋Ｇ、６Ｖ ｖｓ、ＡＱ／Ａｇ
Ｃ１に変化させると酸化電流が流れてその波形が描かれ
る１次いで４０．６から一〇、６Ｖ ＶＳ、Ａ（＋／Ａ
ｇ Ｃ１にサイクリックに変化させると還元電流が流
れてその波形が描かれる。この第１図のボルタモダラム
から波高値を測定する。As is clear from the curve in Figure 1, the potential of action t% is 10, 6 to +G, 6V vs, AQ/Ag with respect to the opposite electrode.
When changing to C1, an oxidation current flows and its waveform is drawn.
When g C1 is changed cyclically, a reduction current flows and its waveform is drawn. The wave height value is measured from this voltamodrum shown in FIG.
第２図は、予め作成した検量線を示す、第１図から得た
波高値と第２図の検量線から含有される塩素イオンの濃
度が得られるのである。FIG. 2 shows a calibration curve prepared in advance, and the concentration of chlorine ions contained can be obtained from the peak value obtained from FIG. 1 and the calibration curve of FIG.
試験結果
次に、表１に本発明による株式会社常光製の１漬の塩素
イオンの濃度再現性を示す。Test Results Next, Table 1 shows the reproducibility of the concentration of chlorine ions in a single dip made by Joko Co., Ltd. according to the present invention.
測定例
［注Ｊ
回 数
平均値 ＝１
慨準変差＝０
変動係数−〇
表 １
塩素イオンの４度
（ｍ ｍｏｌ／１）
１００．９
ｉｏｏ、１
１００．９
１００．４
９９．８
１０１．１
１０１．０
１００．４
９８．９
９９．２
０
００、 ２７
、 ７６８９
、７６７％
測定は同一反応槽内で電極を洗浄しないで連続測定した
。Measurement example [Note J Number of times average value = 1 Standard deviation = 0 Coefficient of variation - Table 1 4 degree of chloride ion (m mol/1) 100.9 ioo, 1 100.9 100.4 99.8 101 .1 101.0 100.4 98.9 99.2 0 00, 27, 7689, 767% Measurements were carried out continuously in the same reaction tank without cleaning the electrodes.
比較例
次に比較のために同一試料について測定電極を膜厚平均
１０μｍで直径はぼ０．０５〜ｌ Ｑ ）１ｍの細孔を
有するポリカーボネート膜で包んで測定した結果を表２
に示す。Comparative Example Next, for comparison, the same sample was measured by wrapping the measurement electrode in a polycarbonate membrane with an average thickness of 10 μm and a diameter of approximately 0.05 to 1 m (Q) pores, and the results are shown in Table 2.
Shown below.
表
塩素イオンの１度
（ｍ ｍｏｌ／Ｉ）
１０１．４
８５．７
７９．２
７４．４
７２．０
測定例
以上のように本発明は優れた反復再現性を示すが本発明
の構成を欠く比較例は全く反復再現性がなく本発明の優
れた作用効果が理解できる。他の生体液を使用しても同
様に優れた反復再現性がえられる。1 degree of surface chlorine ion (m mol/I) 101.4 85.7 79.2 74.4 72.0 Measurement example As described above, the present invention shows excellent repeatability, but lacks the structure of the present invention The comparative examples showed no reproducibility at all, and the excellent effects of the present invention can be understood. Similar excellent reproducibility is obtained using other biological fluids.
［発明の効果］
本発明は、上記したような簡便な操作で、且つ迅速に、
しかも精度よく生体液中の塩素イオンの濃度を測定する
方法を提供するものである。この方法により被検体の状
態を適確迅速に検出できる優れた効果が奏される。[Effects of the Invention] The present invention provides simple and quick operations as described above.
Moreover, it provides a method for accurately measuring the concentration of chloride ions in biological fluids. This method provides an excellent effect of accurately and quickly detecting the condition of the subject.
第１図は本発明の実験結果を示すボルタモダラムである
。第２図は検１線を示すグラフである。FIG. 1 is a voltamodalum showing the experimental results of the present invention. FIG. 2 is a graph showing the test line.
Claims (1)
およびステンレスで構成した対極と、銀／塩化銀で構成
した参照電極とからなる電極を塩素イオンは通過するこ
とができるが、生体液中に含まれる蛋白質などの共存物
質を通過させない、微細多孔を有する高分子膜状物で覆
つて構成した測定電極を設置し、作用電極の電位レベル
を対極に対して−０．６から＋０．６Ｖｖｓ．Ａｇ／Ａ
ｇＣｌに次いで＋０．６から−０．６Ｖｖｓ．Ａｇ／Ａ
ｇＣｌの範囲でサイクリックに変化させ、その電圧変化
に応じて発生する電流変化を連続して得られる酸化波及
び還元波の波高値として測定して、予め作成した検量線
から被検生体液中に含有される塩素イオンの濃度を測定
する方法。 ２、生体液が、血液、血清、血漿、リンパ液、髄液、唾
液から選んだ液である請求項１に記載された、被検生体
液中に含有される塩素イオンの濃度を測定する方法。 ３、生体液が、生体液を緩衝液又は蒸溜水で稀釈くした
液である請求項１に記載された、被検生体液中に含有さ
れる塩素イオンの濃度を測定する方法。 ４、微細多孔を有する高分子膜状物がポリカーボネート
、セルロースアセテート、から選んだ膜状物である請求
項１ないし３のいずれか１項に記載された、被検生体液
中に含有される塩素イオンの濃度を測定する方法。 ５、微細多孔を有する高分子膜状物が５〜７ミクロンの
膜厚である、請求項１ないし４のいずれか１項に記載さ
れた、被検生体液中に含有される塩素イオンの濃度を測
定する方法。 ６、高分子膜状物に配設した微細多孔の直径が０．０３
ミクロン以下である、請求項１ないし５のいずれか１項
に記載された、被検生体液中に含有される塩素イオンの
濃度を測定する方法。[Claims] 1. Chlorine ions pass through an electrode consisting of a working electrode made of silver, a counter electrode made of stainless steel, and a reference electrode made of silver/silver chloride in a biological fluid in the same tank. However, by installing a measuring electrode covered with a polymer membrane with microporous that does not allow coexisting substances such as proteins contained in biological fluids to pass through, the potential level of the working electrode is set relative to the counter electrode. -0.6 to +0.6V vs. Ag/A
gCl then +0.6 to -0.6V vs. Ag/A
gCl is cyclically changed in the range of voltage, and the current change that occurs in response to the voltage change is measured as the peak value of the continuously obtained oxidation wave and reduction wave. A method to measure the concentration of chloride ions contained in. 2. The method for measuring the concentration of chloride ions contained in a test biological fluid according to claim 1, wherein the biological fluid is a fluid selected from blood, serum, plasma, lymph, spinal fluid, and saliva. 3. The method for measuring the concentration of chloride ions contained in a test biological fluid according to claim 1, wherein the biological fluid is a liquid obtained by diluting the biological fluid with a buffer solution or distilled water. 4. Chlorine contained in the test biological fluid according to any one of claims 1 to 3, wherein the microporous polymer membrane is selected from polycarbonate and cellulose acetate. A method of measuring the concentration of ions. 5. Concentration of chloride ions contained in the biological fluid to be tested according to any one of claims 1 to 4, wherein the polymer membrane having micropores has a thickness of 5 to 7 microns. How to measure. 6. The diameter of the micropores arranged in the polymer membrane is 0.03
The method for measuring the concentration of chloride ions contained in a test biological fluid according to any one of claims 1 to 5, which is less than a micron.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP16525889A JPH0331753A (en) | 1989-06-29 | 1989-06-29 | Method for measuring concentration of chlorine ion in biofluid |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP16525889A JPH0331753A (en) | 1989-06-29 | 1989-06-29 | Method for measuring concentration of chlorine ion in biofluid |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH0331753A true JPH0331753A (en) | 1991-02-12 |
Family
ID=15808905
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP16525889A Pending JPH0331753A (en) | 1989-06-29 | 1989-06-29 | Method for measuring concentration of chlorine ion in biofluid |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH0331753A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0643300A1 (en) * | 1993-09-09 | 1995-03-15 | Kodak Limited | Method and apparatus for measuring silver ion activity |
| US11217714B2 (en) | 2018-09-13 | 2022-01-04 | Kabushiki Kaisha Toshiba | Evaluation method on anion permeability of graphene-containing membrane and photoelectric conversion device |
-
1989
- 1989-06-29 JP JP16525889A patent/JPH0331753A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0643300A1 (en) * | 1993-09-09 | 1995-03-15 | Kodak Limited | Method and apparatus for measuring silver ion activity |
| US11217714B2 (en) | 2018-09-13 | 2022-01-04 | Kabushiki Kaisha Toshiba | Evaluation method on anion permeability of graphene-containing membrane and photoelectric conversion device |
| US11682740B2 (en) | 2018-09-13 | 2023-06-20 | Kabushiki Kaisha Toshiba | Evaluation method on anion permeability of graphene-containing membrane and photoelectric conversion device |
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