JPH0334908A - External preparation for skin - Google Patents

Abstract

PURPOSE:To obtain an external preparation for skin, improving chapped skin, having excellent effects on beautiful skin, containing a specific amount of adherent protein derived from shells having byssuses or microorganisms and having a pH in a specific range. CONSTITUTION:An external preparation for skin containing 0.0001-0.5wt.% adherent protein (preferably obtained especially from byssuses of shells and/or phenol gland) and having pH5.0-9.0. Further the external preparation is preferably blended with 1-50 times as heavy L-ascorbic acid or derivative thereof as the adherent protein. The external preparation has cell multiplying effects, improves dryness of skin, has adhesion, good compatibility with skin, can use an adherent substance resistant to sweat and water and is excellent.

Description

DETAILED DESCRIPTION OF THE INVENTION [Industrial Application Field] The present invention aims to provide an external skin preparation that improves rough skin and has excellent skin beautifying effects.

[Conventional technology]

Various methods have been devised so far to improve rough skin and create beautiful skin.

First, hormonal agents have been widely used to promote skin metabolism. However, hormonal agents may have side effects, and their types and amounts are strictly limited.

Furthermore, in order to improve the water retention of the skin, skin cosmetics containing hyaluronic acid, mucopolysaccharide, and soluble collagen (Japanese Patent Publication No. 19725/1983), and specific cosmetics to activate the cell activation effect. External skin preparation containing the three precepts of amino acids, vitamins, and sugars as essential ingredients (JP-A-61
-289016) and cosmetics containing rosmarinic acid that has a wound-healing effect (Japanese Patent Application Laid-open No. 162611/1983).
Various studies have been conducted, such as cosmetics containing fibronectin, a type of glycoprotein (Japanese Patent Application Laid-open No. 76007/1983), but it takes a long time to develop beautiful skin. However, it could not be said that it was sufficient to produce a significant effect in a short period of time. In addition to fibronectin, laminin and collagen are also known as substances that promote cell proliferation, but they are still insufficient.

On the other hand, various adhesive substances applied to the skin have been used so far.

For example, for beer-off type packs, water-soluble ↑)J
High-molecular polyvinyl alcohol is widely used, but it evaporates water slowly and takes time to dry. In addition, when sweating, the adhesion to the skin becomes weaker, resulting in an uncomfortable feeling, so there is a need for the development of new film-forming agents. In addition, acrylic acid ester-vinyl acetate copolymers are commonly used for patch plasters, poultices, and their fixing adhesive sheets, and sweat and moisture weaken the adhesive strength, causing them to peel off easily or over time. as rash, redness, itching,
It was not easy to use, as it sometimes caused allergic symptoms such as a rash.

(Problems to be Solved by the Invention) In order to improve rough skin and create beautiful skin, the present inventors searched for a clay that has an excellent cell proliferation effect and has strong adhesive properties against water, and applied it to the skin. Contain it in external preparations to increase its effectiveness.
l [The objective was to put it into actual use with the turtle, b.

[Means for solving the problem] The present defenders have been researching biological ingredients that can improve this problem and have excellent skin beautifying effects. We were able to obtain an adhesive substance that is flexible, blends well with the skin, and is resistant to sweat and moisture.

However, in the present invention, the adhesive protein is reduced to 0,000.
Containing 1 to 0.5% by weight (1 consisting of 7) 115.5-,-
It is a skin external preparation with a pH of 9.0, and in a preferred embodiment, it is an adhesive protein derived from shellfish byssus and/or phenol glands, and in a more preferred embodiment, it is an adhesive protein derived from shellfish byssus and/or phenol glands. This is an external skin preparation characterized in that the derivative is used in combination in an amount of 1 to 50 times the weight of the adhesive protein.

The present invention will be explained in detail below.

The adhesive protein used in the present invention includes proline residues,
It is an adhesive protein consisting of amino acid residues such as glycine residues, lysine residues, and tyrosine residues, and has a 77 amino acid sequence of approximately 300 to 1200 amino acids.

Suitable adhesive proteins are those derived from shellfish having byssus threads, such as molluscs, bivalves, molluscs, molluscs of the order Pteromorpha, molluscs such as the purple mussel, the rainbow mussel, the lagoon oyster, and the pearl oyster, as well as from microorganisms.

Below, the method of Engebretson (G, H, Enaebr)
etson at at, Comp, Bioch
em, Physiol, 77B[1], 201(
1984), ), we will describe a method for producing adhesive proteins from the byssus threads of Murazakii.

Murazakii Inoj”i is a black-shelled shellfish that grows in dense clusters in tetrapods and concrete seawalls (Isamu Sakaro, Research Report of Biological Research Institute, Central Research Institute of Electric Power Industry, No. 4)
85024. P, 1-17 (1986), ). The byssus consists of a trunk, thread, and sticky disk, which are cut from the shell and washed with seawater to remove foreign substances such as pebbles.

Byssus total 0.05M 1) Sue salt M (pH 7,5)
, 1.0M common salt, 0.001M butylated phenylmedyl sulfol, 0.01M N=edilmaleimide, 0.01M salt.
Stored in a proteolytic enzyme inhibitor solution consisting of 025M ethylenediamine 4 vinegar. Take out the byssus and mix it with 4M guanidine hydrochloric acid and 0.1M 1~lisu (pH 7).
, 2. Then, the bysulia is immersed in this solution and extracted at 5°C for 48 hours. In a centrifuge
[Divided into clear liquid and residue (20,000 x Q). The residue is dispersed in a 0.5M acetic acid aqueous solution, pepsin is added, and the protein is digested at 5°C for 48 hours.

Separate the supernatant and residue using a centrifuge (20,000
XG). The supernatant was fractionated by gel chromatography (Sephadex G-150), and the molecular weight was approximately 15,000.
Pepsin-digested adhesive protein fractions are obtained by collecting fractions of ~150.000.

Furthermore, Waite's method (formerly Waite, J., BiO
IOgical Chemistry, 258 [5L2
911 (1983), and U.S. Patent No. 4.687.74.
0), from the phenol glands of the purple mussel,
]・It is also possible to obtain an adhesive protein by digesting ribsin.

Further, the adhesive protein used in the present invention can be obtained from commercially available products, such as CellTak manufactured by Piobolimers (B10PO1yIIlerS, InC1).

Furthermore, using recombinant DNA technology, microorganisms (E. coli,
Bioadhesion proteins produced using Bacillus subtilis, yeast) as hosts (Japanese Patent Application Laid-open No. 61-85400@) can also be used.

These adhesive proteins are stable near neutrality and p
It can be used at pH 5.5 to pH 9.0, but especially pt+e,
5 to pH 8.0 is suitable. For the purpose of improving rough skin, these adhesive proteins are used in amounts of o, oooi to 0.5% by weight. o, oooi If the concentration is lower than 0.5% by weight, no effect can be expected, and if it exceeds 0.5% by weight, the specific odor will be strong.
Neither is preferable.

Next, the L-ascorbic acid or its derivative used in the present invention refers to the vitamin C group, and especially "-ascorbic acid phosphate ester magnesium salt (hereinafter referred to as AP
〉 is preferred.

By using the adhesive protein and vitamin C group together, the stability of the adhesive protein can be increased and the skin beautifying effect can be further enhanced. If the amount of the vitamin C group is lower than the weight of the adhesive protein, the effect of increasing the stability of the adhesive protein will be reduced, and if the amount of the vitamin C group exceeds 50 times the weight of the adhesive protein, the adhesive protein This would be excessive for the purpose of stabilizing the system, and would be wasteful for the intended purpose.

〔Example〕

Next, the formulations and manufacturing methods of Examples of the present invention and Comparative Examples for comparison will be described.

Incidentally, the blending ratio is in parts by weight.

The formulations of the nutritional emulsions of Example 1 and Comparative Example 1 are summarized in Table 1.

(Manufacturing method) Add ① to a homomixer and stir at room temperature to dissolve uniformly. Add ① to this while stirring to emulsify.

Next, add ■ to neutralize.

After that, add ■ to make a homogeneous emulsion.

Pack into a container and store in a cool, dark place.

Example 2. Ready-to-use lotion (prescription) (manufacturing method) Stir ① at room temperature to uniformly solubilize it and use it as a lotion.

Mix (2) at low speed to obtain powder 1.

(How to use) Take an appropriate amount of the powder (■) in the palm of your hand, add 1 part of the lotion (■), and mix well with your fingers. Apply this to your skin and spread it well.

Example 3. Beer-off type pack fee (prescription) 1.3 Butylene glycol = 1 le paraoxyben'a M methyl (Manufacturing method) Disperse and dissolve (2) at room temperature.

This is often accompanied by ■.

Pack it into a container and use it as a product.

(Effects of the Invention) The fact that the product of the present invention is a skin preparation for external use that improves 1] It and is immersed in the Mitsuki effect will be described in detail.

(1) Experimental method and results for skin beautification effect> 2 (Sample) Crystal of the present invention and comparative product (Test method) 16 subjects exhibiting senile xeroderma were selected, and each was randomly assigned to Group A (8 ), divided into 8 groups (8 people) (B, A f
Group 8 was asked to use Invention Product 1 twice a day (morning and daytime) for 2 months, and the skin condition was visually observed after 2 months.

(Evaluation [i > 41-4-: Most of the roughness has recovered -+-+: Much of the roughness has recovered 10: Somewhat of the roughness has recovered: No change results) As is clear from Table 2, the product of the present invention It can be seen that this method improves Tsuki n Are and is superior to Yaguruma 1 effect.

(2) l] 11 Improvement Effects Experimental Methods and Results> (Samples) Invention Product 1 and Comparative Amount 1 (Test Method) A panel of 20 women with dry or rough skin 1 let.
Comparative amount 1 was applied to the entire back of the left hand, and inventive product 1 was applied to the entire back of the right hand.
Apply each twice a day (morning and evening), stretching well, and after applying this for 20 days, a beauty expert will evaluate the evaluation items shown in Table 3 on a 5-point scale (ml I received it.

Average scores for all panelists are not shown in Table 4.

(Left space below) (Evaluation) (Results) As is clear from Table 4, the nutritional emulsion of the present invention, when used repeatedly on the skin, achieved high scores in all items and achieved the intended purpose. There is.

on the other hand, Ratio without adhesive proteins Calibration amount 1 is Degree of improvement in dryness and fine wrinkles The match is low.

Claims (1)

[Scope of Claims] 1) An external preparation for skin having a pH of 5.5 to 9.0 and containing 0.0001 to 0.5% by weight of an adhesive protein. 2) The skin external preparation according to claim 1), wherein the adhesive protein is obtained from the byssus and/or phenolic glands of shellfish. 3) The skin external preparation according to claim 1) or 2), characterized in that L-ascorbic acid or a derivative thereof is used in an amount of 1 to 50 times the weight of the adhesive protein.