JPH0334913A - Hair cosmetic - Google Patents

Abstract

PURPOSE:To obtain the title excellent cosmetic promoting metabolism of scalp, dyeing white hair into gray, having protecting and recovering effects on damaged hair and split hair, containing a specific amount of adherent protein as an active ingredient. CONSTITUTION:A cosmetic containing 0.0001-0.5wt.% adherent protein as an active ingredient. The adherent protein used is an adherent protein which comprises amino acid residues such as hydroxyproline residue, lysine residue, tyrosine residue or 3,4-dihydroxyphenylalanine residue, has about 300-1,200 amino acid sequence and adhesion and is obtained especially from phenol gland and/or byssus. The adherent protein is obtained by cutting phenol gland of Soletellina diphos, storing the phenol gland in a solution of protease inhibitor, centrifuging, immersing the residue in an aqueous solution containing urea, sodium borate, etc., and collecting the residue.

Description

[Detailed Description of the Invention] [Industrial Application Field] The present invention improves the metabolism of the scalp, dyes gray hair gray, and coats the surface of the hair to protect damaged hair and split ends. The purpose of the present invention is to provide a hair cosmetic that has the effect of restoring hair loss.

[Prior Art] First, various studies have been conducted on methods of increasing scalp metabolism.

For example, scalp stimulants such as capsicum tincture and cantharis tincture have been used, but the amounts used are limited because they can be too irritating if the scalp is damaged.

Blood circulation promoters such as nicotinamide are also used, but their amounts are limited due to skin irritation.

Furthermore, keratolytic agents such as lactic acid and salicylic acid are also used, but they are highly acidic and cause serious skin irritation.

In addition, female hormones are sometimes used, but their types and amounts are strictly limited due to the fear of side effects.

Enzymes are also used in some cases, but they have drawbacks in terms of stability and safety.

Next, as a method to make gray hair less noticeable, hair dyes have been used to dye gray hair from gray to black.

For example, natural organic dyes such as henna and chamomile are used, but their quality varies widely and there are problems with quality control.

Synthetic dyes such as pyrogallol are also used, but
It is toxic and can irritate the skin, so extreme care must be taken when handling it.

Furthermore, inorganic pigments such as black iron oxide and carbon black are also used, but they have the drawback of having weak adhesion to hair and being easily removed by moisture or sweat.

Next, hair treatment agents have been used as a way to protect and restore damaged hair and split ends.

For example, hair treatment agents containing oily ingredients such as 2-octyldodecanol, castor oil, and olive oil may cause stickiness on the hair or make the hair itself feel heavy.

In addition, hair fixatives using polyvinylpyrrolidone, carboxymethylcellulose, acrylic acid resin, etc.
The resultant hair finish tends to be stiff and stiff, and a natural finish cannot be imparted.

Furthermore, hair cosmetics that use a combination of high molecular weight silicone and low boiling point oil make it easier to comb through the hair.

Although it can be expected to have a hair protective effect, its ability to protect and restore damaged hair and split ends is inferior.

[Problems to be Solved by the Invention] The present inventors have discovered an effect that increases the metabolism of the scalp, dyes gray hair gray, and further coats the surface of the hair to protect and restore damaged hair and split ends. The objective was to search for a material that has strong adhesive properties against water, incorporate it into hair cosmetics, confirm its effectiveness, and then put it into practical use.

[Means for solving the problem] The present invention is a hair cosmetic containing 0.0001 to 0.5% by weight of an adhesive protein, and in a preferred embodiment, the adhesive protein is a phenol gland of a shellfish. or/and from the byssus.

The present invention will be explained in detail below.

The adhesive protein used in the present invention consists of amino acid residues such as hydroxyproline residue, lysine residue, tyrosine residue, and 3,4-dihydroxylphenylalane (DOPA> residue), and has an amino acid sequence of about 300 to about 1,20
It is an adhesive protein with a stickiness of 0.

Suitable adhesive proteins are those derived from microorganisms and shellfish having byssus threads, such as mussels of the molluscs Bivalvia, Pteroptera, Mussel family, Rainbow mussels, Laguis mussels of the Laguidae family, and pearl oysters.

Below, the method of weighting (J, eyes, Waite, J.

Biological Chemistry, 258
[5], 2911 (1983).

and US Pat. No. 4,887,740, a method for producing trypsin-digested adhesive proteins from the phenolic glands of mussels will be described.

Murasaki mussels are dark-skinned shellfish that grow in dense clusters in tetrapods and concrete seawalls (Isamu Sakaro, Research Report of Biological Research Institute, Central Research Institute of Electric Power Industry, No. 485)
024. P, 1-17 (1986), ). The phenolic glands of fresh mussels were cut out and treated with 0.05M Tris-HCl (pH 7.5), 1
．． 0 M common salt, O, 0.01 M fermethylsulfonyl buttride, 0.01 M N-ethylmaleimide, 0.0
Store in proteolytic oxygen inhibitor solution consisting of 25 M ethylenediaminetetraacetic acid. This was cut into small pieces using a grinder, passed through a homogenizer, and then placed in a centrifuge.
Collect the residual power and discard the supernatant.

This residual force was dissolved in a 0.01M sodium borate aqueous solution (1) containing 3M urea and 0.01mM calcium chloride (88
．． After soaking at 5° C. for 48 hours, the sample is centrifuged (20,000×G), the residual force is collected, and the supernatant liquid is discarded.

This residual force was dispersed in a sodium bicarbonate aqueous solution (pH 8,0) under a nitrogen gas atmosphere, trypsin was added, and 2
Digest the protein at 5°C for 30 hours. Add glacial acetic acid to acidify and stop the enzymatic reaction.

Centrifuge (20,000XG) and separate into supernatant and residual liquid. The supernatant is passed through a gel to remove trypsin, and an ion exchange resin is used to obtain trypsin-digested adhesive proteins.

Moreover, the adhesive protein used in the present invention can also be obtained from a commercially available product, for example, from BioPolymers, Inc.

)y cell tak (Cel 1-Tak).

Furthermore, using recombinant DNA technology, microorganisms (E. coli,
Bioadhesive proteins produced using Bacillus subtilis and yeast as hosts (Japanese Patent Application Laid-open No. 1985-85400) can also be used.

These adhesion proteins can be used for their intended purposes at 0,000
01 to 0.5% by weight is used. If the concentration is lower than o or oooi% by weight, no effect can be expected, and if it exceeds 0.5% by weight, the specific odor will be strong, both of which are undesirable.

The hair cosmetics of the present invention include hair conditioners, hair sprays, hair tonics, scalp conditioners,
It is a hair dye, etc., and can be manufactured using conventional methods using raw materials commonly used in head and hair cosmetics.

In addition, during actual use, it is important to thoroughly rub the scalp and hair.

[Examples] Next, the formulations and manufacturing methods of Examples of the present invention and Comparative Examples for comparison will be described.

Incidentally, the blending ratio is in parts by weight.

The formulations of the hair conditioners of Example 1 and Comparative Example 1 are summarized in Table 1.

(Margin below) ■, Stir at room temperature to uniformly dissolve.

Add the mixture (2) while stirring and solubilize. Add ■ to the mixture without stirring.

Pack into a container and store in a cool, dark place.

Example 2 Hairspray (formulation) 95% ethanol 76,935 dimethylpolysiloxane 0.5 olive oil
0.5 polymethacrylic acid ester/7.0 ethanol solution (30%) δ-tocopherol o, oi adhesive protein 0.005 scent F4
0.
05 Purified water 15. O (Production method) Uniformly disperse at room temperature.

Fill it into a pump-type spray container and use it as a product.

Example 3. Scalp conditioner (prescription) 0 1 (manufacturing method) Add ■ to a homomixer, stir at room temperature, and dissolve uniformly. Add ■ to this while stirring to emulsify. Next, add ■ to neutralize. do.

Then add ■ to obtain a milky lotion.

Pack it into a container and use it as a product.

[Effects of the Invention] The effect of the product of the present invention on promoting scalp metabolism will be compared from the turnover of scalp stratum corneum cells. In addition, a dyeability test to determine the ability to dye gray hair gray, and a breaking strength test to protect damaged hair will be shown.

(1) Turnover rate of scalp stratum corneum cells <Experimental method and results> (Sample) Invention product 1 and comparative amount 1 (Test method) 2 20 male and female subjects aged 25 to 60 years old are divided into two groups. Referring to Japanese Patent Publication No. 63-106558, the stratum corneum cells of the scalp are peeled off with adhesive tape, stained with gentian violet-brilliant green staining solution, and the area of the cells is measured with an image analyzer.1 For details, refer to the product of the present invention. In another group, a comparative amount of 1.5q of each was applied to the scalp twice a day and used continuously for one month.Again, the same method as at the beginning of the study was used to test the stratum corneum cells of the scalp. Measure and compare areas.

(Results) The area of the group coated with the product of the present invention was reduced by 12%, while the area of the group coated with a comparative amount was reduced by only 4%.

Since it is already known that there is a correlation between the decrease in cell area and cell turnover, it is clear that the product of the present invention promotes scalp metabolism.

(2) Experimental method and results for stainability test> (Sample) 3 Invention product 1 and comparative amount 1 (Test method) Twenty subjects, male and female, aged 40 to 60 with gray hair, were divided into two groups. One group received the product of the present invention, another group received a comparative amount, and 1
A dose of 2.59 doses each was applied twice a day to the scalp and hair, and continued use was continued for 3 months.

Photographs of the condition of the applied area were taken before and after the start of the test, and the degree of grayness was compared relative to each other.

(Evaluation criteria) Significant effect: 10+ Quite effective: 10: Somewhat effective ± No effect) As is clear from Table 2, the product of the present invention, compared to the comparative amount,
You can see that she has dyed her white hair gray.

(3) Experimental method and results for hair breaking strength test (Sample) Inventive product] and Comparative product 1 (Experimental method) 100 female hairs damaged by perm, bleach, hair dye, etc. were collected. The average thickness is the diameter o, o
oa, hair length 5cm), tied into 10 bundles. This was immersed in the sample for 30 minutes, and then dried in a silica gel desiccator at 20°C day and night to obtain a test sample.

Set it in a tensile strength tester (manufactured by Toyosui-Rudoin Co., Ltd.),
The breaking strength of the hair bundle is measured, and the average of 5 measurements is calculated.

(Results) 5 As is clear from Group 3, compared to the comparative product, the product of the present invention has
It was found that the breaking strength was increased, and the product of the present invention was found to be effective in protecting and restoring damaged hair.

As is clear from the above results, the product of the present invention has been confirmed to have the effect of increasing the metabolism of the scalp, dyeing gray hair gray, and protecting and restoring damaged hair and split ends, resulting in excellent scalp hair. It's a cosmetic product.

Claims (1)

[Scope of Claims] 1) A hair cosmetic containing 0.0001 to 0.5% by weight of adhesive protein. 2) The hair cosmetic according to claim 1), wherein the adhesive protein is obtained from phenolic glands and/or byssus of shellfish.