JPH0339726Y2 - - Google Patents

Description

[Detailed Description of the Invention] Industrial Application Field The present invention relates to a support wetting (buffering) device in an electrophoresis device.

Conventional technology As is well known, in electrophoresis, a sample such as serum protein is coated on a support containing a certain pH buffer solution, and when a direct current is applied to both ends of the support, buffer ions and charged particles are collected. By utilizing the fact that molecules or atoms migrate in the direction of the pole opposite to their charge, are arranged on a support in a different order of migration speed, and are separated, each fraction is separated by the difference in separation speed. This method measures the composition of a specimen by quantifying the amount of surface.

In order to carry out the above measurements, each sample is coated on a thin support film moistened with a buffer solution, electrophoresed in an electrophoresis tank, fixed and stained, and each substance separated into the electrophoresis membrane. It is necessary to read the concentration of Therefore, it is essential to first wet the support with a buffer solution for each sample. Conventionally, this wetting step has been carried out by rotating and moving a sponge roller containing a buffer solution along the surface of the support film.

Problems to be solved by the invention However, in the conventional wetting method, there is a problem in that wrinkles occur on the film surface of the support due to contact with the sponge roller, causing various troubles in subsequent steps.

It is known that a feature of cellulose acetate membranes, which are widely used as supports, is that wrinkles do not occur when the membrane is wetted while being fed through a buffer solution at a moderate speed.

The present invention was devised in view of the above points, and aims to eliminate wrinkles that occur on the support during the wetting process, so that a good wet state can be obtained at all times.

Means for Solving the Problems In order to achieve the above object, the present invention includes a buffer solution tank and a support disposed in the buffer solution tank with a predetermined curved shape concave toward the bottom. with a guide of
A pair of feed rollers sends the support into the buffer solution tank and sends it along the guide, and a pair of feed rollers that pinch the wet support sent along the guide and send it out of the buffer solution tank, and remove excess buffer in the sending process. A configuration comprising a pair of squeezing rollers for squeezing out the liquid was adopted.

Operation The support supplied to the device is sandwiched between the feed rollers placed on the inlet side of the buffer solution tank, and by the rotation of the rollers, it is sent into the buffer solution tank, and then sent along the guide to the exit side at an appropriate speed. It will be done. During the feeding process, the support is wetted with a buffer solution. The support body sent to the exit side along the guide is sandwiched between squeezing rollers, and sent out to the next process (electrophoresis processing process) by the rotation of the rollers, and during the sending out,
Excess buffer wetting the support is squeezed out.
Therefore, the support sent out from the wetting device is evenly, uniformly, and appropriately wetted.

Embodiments Hereinafter, embodiments of the present invention will be described with reference to the drawings.

FIG. 1 shows a partial cross section of the electrophoresis device according to the present invention, in which a paper feed unit 10 is installed in the upper part of the inside of the device.
is provided, and one by one is taken out one by one from among the plurality of supports A housed in the paper feed cassette 11 by the rotation of the rotating roller 12 and sent out. When two or more supports A are stacked on top of each other, they are discharged out of the apparatus by the trouble ejecting unit 20, and when only one support member A is present, they pass through the same unit 20.

A wetting unit 30 is provided in the lower part of the apparatus, below the discharge unit 20.

The wetting unit 30 includes a buffer tank 31, a support guide 32 disposed inside the buffer tank 31 with a predetermined curved shape concave toward the bottom, and a buffer tank located below the discharge unit 20. It consists of a pair of support feed rollers 33, 33 disposed on the inlet side of the buffer solution tank 31, and a pair of squeezing rollers 34, 34 disposed facing the support outlet side of the buffer solution tank 31.

The support A, which has passed through the discharge unit 20 and is sent downward, is first caught between the feed rollers 33, 33, and then sent into the buffer tank 31 by the rotation thereof, and is moved along the curved shape of the guide 32. It is sent to the exit side as shown by the arrow. During the feeding process, the support A is wetted by the buffer solution 100 filled in the buffer solution tank 31. At that time, the feed rollers 33, 3
It goes without saying that the feed rate of the support A by the rotation of step 3 is set at an appropriate value.

The support A sent along the guide 32 to the exit side of the buffer solution tank 31 is first sandwiched between squeezing rollers 34, 34, and then sent out to the serum application unit for the next step by rotation thereof. In the process, support A
The excess buffer solution 100 moistened by the squeezing roller 3
4 and 34. As a result, the support A that has passed between the squeezing rollers 34, 34 is kept evenly and uniformly and properly moistened.

Effects of the invention As explained above, according to the invention, the support is guided through the buffer solution at an appropriate speed by the rotation of the feed roller, and the support after leaving the buffer tank is moved by the squeezing roller. As a result of this action, an even, uniform, and appropriate moist state is maintained, so wrinkles do not occur during the wetting process, and a good moist state is always obtained.

[Brief explanation of the drawing]

FIG. 1 is a sectional view of the device according to the present invention. 31... Buffer tank, 32... Guide, A... Support, 33, 33... Feed roller, 34, 34...
...Aperture roller.

Claims (1)

[Scope of utility model registration request] a buffer solution tank; a support guide disposed in the buffer solution tank to have a predetermined curved shape concave toward the bottom; the support body is fed into the buffer solution tank, and is guided along the guide; A pair of feeding rollers for feeding, and a pair of squeezing rollers for pinching the wetted support fed along the guide, sending it out from the buffer solution tank, and squeezing out excess buffer solution in the sending process. A support wetting device in an electrophoresis device consisting of: