JPH0344554A - Filtration jig and filtration device - Google Patents

Abstract

PURPOSE:To facilitate mechanical and manual operations for suction and to make it possible to meet wet fixation in a simple manner by opening a filtration port in the bottom of a jig for filtration and also by opening a suction port in the lateral side part thereof. CONSTITUTION:After a water retaining piece 3 is built in the main body 4 of a jog, a filter body 2 is built therein. Next, an injecting body 1 is built in the main body 4 of the jig. On the occasion, the injecting body 1 is pressed elastically against the main body 4 of the jig by a retaining piece 12 and retained thereby, and the lower side is sealed hermetically by an O ring. Next, a sample solution is injected into an injecting body 1 and sucked through a filtration port 7 and a suction port 8 and a specimen is caught on a filter. On the occasion of suction, an open state is brought about by pressing an opening-closing pin 9 in the filtration port 7 or a sphere 11 in the suction port 8 against an elastic force of an elastic body 10 by a manual suction unit or a nozzle port of a suction device, and the suction is conducted. After the filtration, the filter body 2 is taken out by a pincette or the like. On the occasion when the specimen is set in wet fixation, the opening-closing pin 9 in the filtration port 7 is pushed upward by a slender rod or the like, the water retaining piece 3 is pushed by the pin and the water retaining piece 3 and the filter body 2 are taken out together.

Description

[Detailed Description of the Invention] (Industrial Application Field) The present invention deals with a filtration jig for capturing specimens such as cells by filtration on a filter having countless micropores, and the filtration jig. This invention relates to a filtration device.

[Conventional technology]

In order to microscopically examine a specimen such as a cell, it is necessary to prepare a preparation in advance, and conventionally, this preparation has been prepared by the so-called smear method. However, since this smear method involves manually applying the specimen onto a glass slide, it is inefficient and requires a high level of skill.

In view of these problems, the inventor of the present invention first invented a filter body holder (Japanese Patent Application No. 250188/1988). In this method, a membrane filter having numerous micropores is held in a holder, a solution is filtered through this filter, and only the specimen in the solution is captured on the filter. A filtration hole is opened in either the bottom surface or the side surface of the holder, and the solution is sucked through the filtration hole by a suction device, filtered, and the specimen is captured.

[Problem to be solved by the invention]

A problem with these conventional techniques is that the filter holes are opened on either the bottom or the side surfaces, and the filter holes are limited to those for a suction device or those for manual operation. When capturing a large number of specimens to create a large number of preparations, it is advantageous to mechanically process them using a suction device, and when capturing a small amount of specimen, it is advantageous to process them mechanically without using a suction device. It is more convenient to capture the specimen by hand using a manual aspirator such as a syringe. However, in the conventional holder, the filtration hole is opened only on one side or the bottom surface, and the filtration hole is limited to one of two mechanical devices and one for manual use, which is inconvenient to use. For these reasons, there has been a strong desire for a filtration jig that can be used both mechanically and manually.

In addition, in preparation of preparations, there are two methods: dry fixation, in which the specimen is prepared in a dry state, and wet fixation, in which the specimen is prepared in a wet state. In some cases, it is necessary to constantly supply moisture to the sample on the filter that has been removed from the holder, resulting in the inconvenience of requiring extra work.

The present invention was devised in view of these problems.1 It provides a filtration jig that can be easily used for both mechanical suction devices and manual suction operations, and can also be easily used for wet fixation. That is the issue. Another object of the present invention is to provide a filtration device that performs suction filtration by assembling this filtration jig.

[Means to solve the problem]

As a means for this purpose, a cylindrical injection body 1 into which a solution containing a specimen is injected, a filter body 2 in which a filter 6 having numerous micropores is attached to a frame body 5, and a thin plate-like injector with high water retention properties are used. It has a water retaining piece 3, and a filter hole 7 extending vertically through the center, and a suction hole 8 that communicates with the filter hole 7 and opens on at least one side. It consists of a filter body 2 and a jig main body 4 which holds the injection body 1 in a detachable manner.

It is necessary to provide a check valve mechanism in the filtration hole 7 and the suction hole 8, but this check valve mechanism is made by fitting and assembling a so-called commonly used check valve into the filtration hole 7 or the suction hole 8. It can be configured.

However, in order to further improve the sealing performance, the check valve mechanism in the filter hole 7 is constructed by providing an opening/closing pin 9 and a bullet 10 in the filter hole 7, and pressing the opening/closing pin 9 downward with one bullet 10. The filter hole 7 is closed and the opening/closing pin 9 is pressed upward to open the filter hole 7. When the opening/closing pin 9 is further pressed upward, the opening/closing pin 9 presses the water retaining piece 3 to retain water. It is preferable that the piece 3 be detached from the jig main body 4.

In addition, the check valve mechanism inside the suction hole 8 is provided with two spheres 11 and a bullet 10 or an opening/closing pin and a bullet 10, and the suction hole 8 is closed by pressing the sphere 11 or the opening/closing pin 9 with one bullet 10. At the same time, the suction hole 8 is opened by pressing the sphere 11 or the opening/closing pin 9 against the elasticity of the elastic material IO.
It can also be used to open up the

Furthermore, a pair of holding pieces 12 that press and hold the injection body 1 can be provided on opposing sides of the jig main body 4, and a positioning piece 12 for achieving positioning of the filter body 2 can be provided on the top surface of the jig main body 4. It is good to provide a piece 13.

Furthermore, it is preferable to form a fitting groove 15 into which the water retaining piece 3 fits on the upper surface of the jig main body 4, and to protrude a plurality of supporting pieces 16 around the filter hole 7 to support the water retaining piece 3 from below. An O-ring 17 whose lower end is in close contact with the upper surface of the filter body 2 is provided at the lower end of the injection body 1 to tightly hold the water retaining piece 3 in the fitting groove 15 of the jig main body 4 and whose upper end is attached to the filter body 2. It is preferable to provide a seal 18 that closely contacts the lower surface. A take-out groove 14 for taking out the filter body 2 may be formed on the upper surface of the jig main body 4.

Also, a filtration device that filters the solution inside the filtration jig.

A fixing base 19 for removably fixing the filtration jig, a nozzle 20 whose nozzle opening 23 at the upper end is removably assembled into the filtration hole 7 at the bottom of the filtration jig, and a nozzle 20 that can be fixed and moved up and down. , a lifting table 21 that fits and assembles the nozzle port 23 into the filter hole 7 in the raised state, and detaches the nozzle port 23 from the filter hole 7 in the lowered state; connected to the bottom end.

It consisted of a suction device 22 that filters the solution in the filter jig.

In this filtration device, the suction device 22 may be a roller pump, and a fixing liquid injection mechanism may be added.

[Effect]

First, the function of the filter jig will be explained.

First, after assembling the water retaining piece 3 to the jig main body 4, the filter body 2 is attached to the jig main body 4. This can be reliably achieved by disposing a packing 18 in the fitting groove 15 to tightly hold the water retaining piece 3. Also, the filter body 2
The assembly can also be achieved accurately by providing the positioning piece 13 on the top surface of the jig main body 4.

Next, the injection body 1 is assembled into the jig main body 4. In the jig body 4,
By providing a holding piece 12 that presses and holds the injector 1, and by providing an O-ring 17 at the lower end of the injector 1 that tightly contacts the upper surface of the filter body 2, a tight assembly that does not cause liquid leakage is achieved. You can.

After the assembly of the filter jig is completed, a solution containing the specimen is injected into the injection body 1. After the solution is injected, the solution is suctioned and filtered, and the sample is captured on the filter 6. In the case of the present invention, not only the filter hole 7 is opened in the bottom of the jig main body 4, but also the suction hole 7 is formed in the side surface of the jig body 4. Since the hole 8 is opened, a manual suction device such as a syringe can be inserted and a suction operation can be performed while visually confirming the suction hole 8, thereby making the work extremely easy.

When sucking from the filter hole 7 or the suction hole 8.

Opening/closing pin 9 or sphere 11 in filter hole 7 or suction hole 8
1 by pressing in a direction against the elasticity of the elastic material 10 with a manual suction device or nozzle port 23 of a suction device to open it and suck it. By this suction operation, the solution in the injection body 1 is filtered and discharged by the filter 6 having numerous micropores, and only the specimen is captured on the filter 6.

After capturing the specimen, when removing the filter body 2 from the jig main body 4, if the specimen is to be dry-fixed, the filter body 2 is held and removed with tweezers or the like. In this case, if a take-out groove 14 is provided in the jig main body 4, one side of the tweezers can be inserted into the take-out groove 14, so that the filter body 2 can be easily held.

Conversely, when wet-fixing the specimen, use the opening/closing pin 9 inside the filter hole 7.
When pressed upward from the bottom side with a thin stick or the like, the upper end presses the water retaining piece 3 and the water retaining piece 3 and filter body 2 can be removed together. Even if the integrated body of the water retention piece 3 and the filter body 2 is left removed, the moisture in the water retention piece 3 will always soak the sample in the filter body 2, so the sample will not dry out, and therefore Perfect wet fixation is possible.

After the specimen is captured on the filter body 2, it is placed on a semi-transparent slide glass (see Japanese Patent Application No. 75701/1988 previously filed by the applicant) either after staining or as it is. Do a microscopic examination. In that case, by the light diffusing effect of the translucent slide glass.

The fine pores of the filter 6 are not visible, so only the specimen can be examined under a microscope. As a result, anyone can easily create slides without relying on the conventional smear method that requires a high degree of skill and has extremely low one-work efficiency.

Next, the operation of the filtration device will be explained.

First, the filter jig is fixed to the fixing table 19. Next, lift platform 2
1 and push up the opening/closing pin 9 of the filtration jig with the nozzle opening 23 to open the filtration hole 7, and in this state, the fitting is assembled. After injecting the solution into the injection body l.

The suction device 22 is operated to suction and discharge the solution from the filter hole 7, thereby capturing the specimen.

When the capture of the specimen is completed, the fixing table 19 is lowered, and then
Remove the filtering jig, fix a new filtering jig, and perform the operations described above.

〔Example〕

An embodiment of the filtration jig according to the present invention is shown in FIGS.

The jig body 4 is made of synthetic resin and has a substantially rectangular parallelepiped shape.

A filter hole 7 passes through its center. A support tube 26 having a plurality of support pieces 16 formed at the upper end thereof is fitted into the filter hole 7, and an elastic material 10 and an opening/closing pin 9 are arranged inside the support tube 26. The fitting groove 15 formed on the top surface of the filter jig is
It is sloped downward toward the filter hole 7, so that the filtered content liquid can easily enter the filter hole 7.

The suction hole 8 communicates with the filter hole 7 via a communication hole 29 . The suction hole 8 is provided with a ball 112, a bullet material 10, and a seal member 40 made by Junsei Co., Ltd., and the suction hole 8 is closed by pressing the sphere 11 against the seal member 40 with the elasticity of the bullet material. The sphere 11 is pressed by a manual suction device or a nozzle 20 of a suction device to open the suction hole 8 and perform suction filtration. Incidentally, instead of this sphere 11, the filter hole 7
It is also possible to use an opening/closing pin 9 such as that used in

The positioning pieces 13 for setting the mounting position of the filter body 2 are provided in six protruding stages. Of these, four symmetrically provided protruding pieces are used to set the vertical position, and the remaining two are used to set the horizontal position.

A pair of holding pieces 12 are provided, and the upper parts are fixed to the opposite sides of the jig main body 4 by a shaft pin 27, and the lower parts are pressed outward by a spring 28. This spring 2
The holding piece 12 is actuated by the elasticity of the water retaining piece 8 to hold the water retaining piece 3. A convex portion 24 is formed at the head of this holding piece 12, and a concave portion 25 is formed at the bottom of the injection body 1. The convex portion 24 presses the concave portion 25 downward, thereby retaining water. Piece 3 can be held firmly.

This injection body l is made of hard resin and has a substantially cylindrical shape. A circumferential groove is formed at the lower end of the inner circumferential surface of the injection body 1, and an O-ring 17 made of soft resin is attached to the circumferential groove. The lower end of this O ring 17 is located below the lower end of the injection body 1 and is in close contact with the frame 5 of the filter body 2. By doing so, it is possible to prevent the solution inside the injection body I from leaking out (see FIG. 5). Incidentally, the injection body I may be made transparent or translucent, and a scale may be engraved on its peripheral wall to visually measure the amount of solution to be injected.

The filter body 2 is composed of a rectangular thin plate-like frame body 5 with a wall thickness of 0.1 to 0.2 J and a large opening in the center, and a membrane filter 6 stretched over the opening. . This membrane filter 6 has a wall thickness of several tens of microns and has countless fine straight holes of several micrometers in size. A solution containing a specimen is filtered by this filter 6, and specimens larger than the micropores are captured on the filter 6.

The water retaining piece 3 has a circular shape that fits into the fitting groove 15 of the jig main body 4, and is made of a material with high water retaining properties.

FIG. 6 shows another embodiment of the filtration jig of the present invention.

In this embodiment, the support tube 26 and the support piece 16 are not provided, and the bottom surface of the fitting groove 15 is also made substantially flat, which is a simple structure.

Further, unlike the above two embodiments, a configuration in which a plurality of jig bodies 4 are arranged in series can also be adopted. In this case, each unit may be detachable, or a plurality of units may be fixedly assembled as shown in FIGS. 7 and 8. In the case of this embodiment, two jig bodies 4 are fixedly assembled, and the filter body 2 also has two openings formed in one frame 5, and a filter 6 is stretched over the openings. There is.

Figure 7.8.9 shows an embodiment of the filtration device of the present invention.

The fixing base 19 is immovably fixed between the first support shaft 31 and the second support shaft 32, and forms an assembly step portion 34 on which four filter jigs are detachably assembled. This fixed table 19 can be used as a work table by setting its dimensions large. Furthermore, by making the fixed base 19 transparent, it is possible to see through the members located below the fixed base 19,
This allows you to visually check the state of suction.

Immediately above the fixing base 19, a gripping base 30 is provided, which is similarly constructed between the first support shaft 31 and the second support shaft 32, and which fits and grips each injection body 1.

The lifting platform 21 is slidably assembled between a first support shaft 31 and a second support shaft 32. First and second support shafts 31.3
A spring 36 is installed between the elevating table 21 of 2 and the fixed table 9.
is provided to press the elevator platform 21 downward. Lifting platform 21
A push-up member 33 is attached to the push-up member 33, and by rotating the push-up member 33, the lifting platform 21 is moved upwardly against the elasticity of the spring 36. Lifting platform 21
Four nozzles 20 are assembled in the lifting platform 21.
As the nozzle 20 moves upward, this nozzle 20 also rises,
The nozzle port 23 pushes up the opening/closing pin 9 of the filtration hole 7 of the jig body to open the filtration hole 7 and is assembled so as to be suctionable.

Incidentally, the lifting and lowering movement of the lifting platform 21 can be achieved not only manually using the push-up member 33 but also by mechanical means such as a cylinder.

After suction filtration is completed, by rotating the push-up member 33 in the opposite direction, the elevating table 21 is lowered by the elasticity of the spring 36, and the nozzle port 23 is removed from the filter hole 7. A coil spring 35 is attached to each nozzle 20.

The assembly between the nozzle port 23 and the filter hole 7 is made strong, and there is some play in the assembly position.

The nozzle 20 is connected to a roller pump, which is a suction device 22 . A tube 39 made of an elastic material connected to the lower end of the nozzle 20 is handled by a roller 38 that rotates when a handle 37 is turned, thereby sucking the solution in the filtering jig. Note that the suction means is not limited to this roller pump, and for example, well-known means such as an air pump can be used.

Since fixation such as Papanicolaou staining involves wet fixation, the captured specimen must never be allowed to dry or dry, and therefore it is necessary to quickly supply a fixative for capture. 7th
In the apparatus shown in Figure 8, the fixative solution is manually injected into each filtration jig one by one after the sample has been captured.

This operation can be quickly carried out by assembling the fixing liquid injection mechanism shown in FIG. 12 to the filtration device, for example. In this injection mechanism, a plurality of syringes 43 are detachably assembled radially from the center of the rotating table 41, and each set of syringes 43 is set to be located directly above each filter jig.

Operate the lever 45 to press the syringe head 44 with the pressure piece 42.
is pressed to inject the fixative solution in the syringe 43 into the filter jig. By providing this injection mechanism,
The fixative can be injected into multiple filtration jigs at once, and is effective for injecting the fixative for wet fixation, which requires quick fixation. By rotating the two-turn table 41, each set of syringes 43 can be successively positioned directly above the filter jig.

In the above embodiment, the solution is mechanically sucked through the filter hole 7 by the device, and the solution is manually sucked through the suction hole 8.
It is also possible to insert a syringe into the syringe and perform suction manually, or it is also possible to fit the nozzle of a suction device into the suction hole 8 and perform suction. The filtration jig of the present invention is capable of handling both.

[Effects of the Invention] As described above, the filtration jig according to the present invention has the filtration holes on the bottom and the suction holes on the side, so that suction can only be carried out through the filtration holes with a mechanical device. Since suction can be easily performed using a manual suction device through the suction hole on the side, it is possible to perform suction filtration operations as needed, which is extremely convenient.

In addition, by providing an opening/closing pin in the filtration hole and pressing the opening/closing pin upward, the water retaining piece and the filter body can be removed as one unit, which is extremely advantageous for keeping the specimen in a moist and fixed state. .

Similarly, the filtration device according to the present invention was invented to carry out the filtration operation using the filtration jig of the present invention, and the two devices have an excellent effect in that the suction filtration operation of the filtration jig is extremely easy. demonstrate.

[Brief explanation of drawings]

FIG. 1 is a front sectional view showing an embodiment of the filtration jig of the present invention;
Fig. 2 is a side view thereof, Fig. 3 is a plan view thereof, Fig. 4 is a plan sectional view thereof, Fig. 5 is a partially enlarged view of Fig. 1, and Fig. 6 is a front sectional view showing another embodiment. , FIG. 7 is a plan view showing still another embodiment, and FIG. 8 is a side view thereof. FIG. 9 is a partial front view showing one embodiment of the filtration device of the present invention, FIG. 10 is a side view thereof, and FIG. 11 is a plan view thereof. FIG. 12 is a front view showing an embodiment of the injection device assembled into the filtration device. Explanation of symbols Injection body, 2: Filter body, 3: Water retention piece. Jig body, 5: Frame body, 6: Filter filtration hole,
8: Suction hole, 9: Opening/closing pin. Bullet material, 11: Sphere, 12: Holding piece. Positioning piece, 14: Take-out groove, 15: Fitting) 14° support piece, 17: O-ring, 18: Packing. Fixed table, 20: Nozzle, 21: Lifting table. Suction device, 23: Nozzle 0.24: Convex/concave portion, 26: Support tube, 27: Axis pin spring, 29: Communication hole, 30: Grip base. First support shaft, 32: second support shaft. Push-up member, 34: Assembly stepped portion. Coil spring, 36: Spring. Handle, 38: roller, 39: tube. Seal member, 41: rotating table, 42: pressure piece. Syringe, 44: Syringe head. Renoku - Gomura 441-; @ to 44 2--- Phil 7-i $ 3- (Water Agency 4---; it is not 5--- Frame f book 6--
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Claims (12)

[Claims] (1) A cylindrical injection body into which a solution containing a specimen is injected (1
), a filter body (2) in which a filter (6) with numerous micropores is stretched over a frame body (5), a thin water-retaining piece (3) with excellent water-retaining properties, and a filter body (3) arranged vertically in the center. It has a filter hole (7) passing through it, and a suction hole (8) that communicates with the filter hole (7) and opens on at least one side, and the water retaining pieces (
3) A filtration jig consisting of a filter body (2) and a jig body (4) that removably holds the injection body (1). (2) The filter jig according to claim 1, further comprising a check valve mechanism provided in the filter hole (7) and the suction hole (8). (3) Connect the check valve mechanism inside the filter hole (7) using the opening/closing pin (9).
and a bullet (10), and the filter hole (7) is opened by pressing the opening/closing pin (9) downward with the bullet (10).
), and the opening/closing pin (9) is pressed upward to open the filter hole (7), and when the opening/closing pin (9) is pressed upward, the opening/closing pin (9) is closed. 3. The filtration jig according to claim 2, wherein the filtration jig presses the water retaining piece (3) to separate the water retaining piece (3) from the jig main body (4). (4) The check valve mechanism in the suction hole (8) is provided with a sphere (11) and a bullet (10) or an opening/closing pin (9) and a bullet (10), and the bullet (10) The suction hole (8) is opened by pressing the sphere (11) or the opening/closing pin (9).
4. The suction hole (8) according to claim 2, wherein the suction hole (8) is opened by pressing the sphere (11) or the opening/closing pin (9) against the elasticity of the elastic material (10). Filtration jig. (5) The injection body (1) is placed on the opposite side of the jig body (4).
Claim 1 comprising a pair of holding pieces (12) for holding the
, 2, 3 or 4. The filtration jig according to . (6) The filtration jig according to claim 1, wherein a positioning piece (13) for positioning the filter body (2) is provided on the upper surface of the jig body (4). (7) A take-out groove (14) for taking out the filter body (2) is formed on the top surface of the jig body (4).
6. The filter jig according to 2, 3, 4, 5 or 6. (8) Form a fitting groove (15) into which the water retention piece (3) fits on the top surface of the jig body (4), and support the water retention piece (3) from below around the filter hole (7). Multiple support pieces (16
8. The filtration jig according to claim 1, wherein the filtration jig has a protruding portion. (9) Attach the lower end of the injection body (1) to the filter body (
2) An O-ring (17) is provided that tightly contacts the upper surface of the jig body (4) to tightly hold the water retaining piece (3) in the fitting groove (15) of the jig body (4), and the upper end is attached to the lower surface of the filter body (2). Claims 1, 2, 3, further comprising a packing (18) that is in close contact with the
The filter jig according to 4, 5, 6, 7 or 8. (10) Fixing stand (19) that removably fixes the filter jig
and a nozzle (20) whose upper end nozzle opening (23) is removably assembled into the filter hole (7) on the bottom of the filter jig, and the nozzle (20) is assembled and fixed and moves up and down and rises. In the lowered state, the nozzle port (23) is fitted and assembled into the filter hole (7), and in the lowered state, the nozzle port (23) is removed from the filter hole (7). A filtration device comprising: a lifting platform (21); and a suction machine (22) connected to the lower end of the nozzle (20) to filter the solution in the filtration jig. (11) The filtration device according to claim 10, wherein the suction device (22) is a roller pump. (12) A rotary table (41) on which a plurality of syringes (43) containing a fixative solution are removably assembled in a radial manner so as to be positioned directly above the filtering jig, and a syringe head (44) of the syringe (43). 12. The filtration device according to claim 10 or 11, further comprising a fixing liquid injection mechanism comprising a pressure piece (42) that presses the pressure member (42) by actuation of a lever (45).