JPH0349680A - Microbacterium mutation strain 851r and preparation of 851 nutrition solution by application of said strain - Google Patents

Abstract

PURPOSE: To obtain a monocytic protein nutrient solution through a simple industrial fermentation process by using a specific Microbacterium mutant as productive strain by the use of a soybean powder medium or starch medium.

CONSTITUTION: This monocytic protein nutrient solution is produced by using bacteria; wherein Microbacterium mutant 851R is used as productive strain, a soybean powder medium or starch medium is used as productive medium for producing this solution through fermentation. This mutant 851R has the ability to convert a phytoprotein, esp. soybean protein, to monocytic protein. Furthermore, the mutant is a Gram-positive bacterium, nonmotile, capable of producing H₂S, positive in nitrate reduction, and viable in a milk when heated at 60°C for 30 min or at 72°C for 15 min. The color of this nutrient solution becomes light at 38°C and colorless at 40°C. In particular, the DNA of the above mutant contains R-fragment which is detectable by a specific R-fragment DNA probe.

COPYRIGHT: (C)1991,JPO

Description

DETAILED DESCRIPTION OF THE INVENTION The present invention relates to an industrial fermentation method using the strain as a production strain and a method for producing a nutrient solution.

Today, many methods are being attempted to select microorganisms that can be employed in industrial fermentation to produce single-cell proteins that are easily absorbed by the human body.

The inventors have published a method for producing a Se-rich single-cell protein and vitamin E nutrient solution and a bacterial fertilizer containing ammonia-resistant Azotobacter.

This method provides cultured Azotobacter with ammonia tolerance and nitrogen fixation capacity that allows the use of atmospheric nitrogen to produce single-cell protein solutions rich in Zn, Se and vitamins by conventional industrial fermentation methods. An object of the present invention is to use strain 851R as a production strain and design a medium suitable for culturing strain 851R and propagating strain 851R.

Soybeans are a type of protein-rich food.

It is difficult to do so. However, after digestion by bacteria of strain 851R, soy protein is converted into a single cell protein nutrient solution. Is the nutrient solution human? It is a kind of nutritional solution that is not only an easily digestible protein source, but also has the effect of improving the health of the central nervous system, enhancing the organism's immunity, regulating endocrine function, anti-aging, and inhibiting the growth of certain cancer cells. Configure. The microorganism of the present invention is microorganism mutant strain 851R.

The main characteristics of this strain are that it is a Gram-positive bacterium, is non-motile, has nitrate reducing ability, is capable of producing B■S, and can be heated to 63°C for 30 minutes or 72°C for 15 minutes. The reason is that it survives in the milk. Its thallus (
microorganisms) are red or orange-red. However, the color of the thallus turns pink at about 38°C and turns colorless at 40°C. In particular, the DN^ of this strain contains an R fragment detectable with a specific R fragment DN^ probe.

The invention further relates to an industrial method of using bacteria to produce single-cell protein nutrient solutions.

In this method, the microbial strain 851R cultured according to the present invention is used as a production strain, and a soybean medium or a starch medium is used as a production medium.

It is a mutant strain. The main characteristics are Gram-positive rods, non-motile, ability to assimilate glycerol raffinose, ability to reduce nitrate, ability to generate 2S, heated to 63°C for 30 minutes or 72°C for 15 minutes. It is to survive in the milk. The thallus is red or orange-red, and the color of the thallus becomes lighter at 38°C and becomes colorless at 40'C. In particular, the DNA of the strain contains a specific R fragment l-
RParklawn Dr detectable with DN^probe
ive, Rockville, Marylad 2
^merican Type Cul located at 0852
ture Collection ^, T. C. C. It has the essential characteristics of the sample deposited under deposit number 53981.

Strain 851R has distinct characteristics that distinguish it from other species of the microbial genus. Applying strain 851R as a production strain and applying the nitrogen source-containing medium designed and used in the present invention produces a single-cell protein solution rich in peptides, various trace elements and vitamins (listed in the culture analysis report). can do. The culture solution contains more than 20 types of amino acids. This culture solution 100z1 contains amino acids 593-41
72B, vitamin EO. 3-1. Owg, vitamin 80
．． 1-0.3 mg, vitamin PI" 0.5-1.Ozg
, Se1.0-5.0ppm, Zn10-20pp+
a. Mo5.0-10ppm, Co5. (1~10pp
u+, Mn5-15ppm and Cu3. O・〜LOp
Contains pa+.

The weight composition of the medium prepared and used in the present invention is shown below.

1. Soybean medium Soybean powder 5-10% or soy milk (calculated as soybean weight) 5-20%? are various legumes, sunflower seed cake yeast extract or yeast powder CaCO3 K2RPO. MgSO4 NaC I Na2Mob. Na2SeO- ZnSO, CoCl 2. Starch medium Raw materials containing starch (sweet potatoes, potatoes, etc.) or dry materials containing starch (corn powder, etc.) Yeast extract K.HPO. 5-10% 0.02-0.5% 0.05-0.5% 0.02~0.2% 0.01~0.08% 0.01~O.OS% 5.0~50ppm 1.5~10ppm 2.5~50ppn+ 2.5~20ppm 10~20% 1~ 10% 0.04~0.08% 0.05~0.1% MgS04 0.0
2 ~0.04%CaCOz
0.05-0.5% NaCI
0.02-0.04% Na
Jo04 10-2
0pp+I1Na2SeOz
1.5~20ppmZnSO42,5~20p
p+n COC12 5-20
Appropriate amounts of other elements required for ppm human body may be added to the above medium. The amount of the element ranges from 1.5 to 100 ppm. The method for producing a nutrient solution utilizing strain 851R includes the step of aerating the culture in a conventional tank fermenter equipped with an agitator and gas transfer, and optionally the fermenter to homogenize the culture. Provision may be made to shake the culture for the purpose of incubation, and the culture may also be aerated with sterile air to meet the oxygen requirements of bacteria and other microorganisms during the incubation period. ．． 8 used as production strain
The 51R strain grows in the medium prepared according to the present invention. After three stages of cultivation (first, second and third seed cultivation), the thallus is inoculated into a previously autoclaved production medium and incubated for 36-64 hours. During the cultivation period, sterile air was passed through the medium at an aeration rate of 1:0.5-1. Ovvm, stirring speed 180
-260 rpm and culture temperature 28-40'C.

After the end of incubation, the culture broth is autoclaved and harvested. Next, the culture solution is directly packaged into products, and various products can be obtained by different processing methods depending on various needs. Precipitation and 15000rp+I1
, centrifugation at pressure -0.2 to -0.4 g/c II 12 N
Afterwards, a cola-type beverage can be produced from the culture solution. After concentration, the culture fluid becomes concentrated nutrients. After oven drying or spray drying, the culture liquid is in powder form, which can be further processed into capsules or tablets. For spray drying, the inlet temperature is 80℃~300℃,
The outlet temperature is 60°C to 120°C. The broth can be added to flour instead of water and eggs to bake bread and cakes or other foods. The culture solution can also be used in the production of cosmetics.

851 nutritional solution has anti-aging effects, improves constitution, and maintains liver and stomach in good condition. The invention will be explained in detail through experiments below. Male Kunming mice weighing 17.8±1.75 g were used and divided into several groups according to body weight. 1 for each group
I took precautions starting from zero. Test mice were given 851 nutrient solution (abbreviated as 851NS) instead of tap water for 13 consecutive days and fasted on the 14th day. Half of these test mice were then given tap water instead of 851NS. The other half of the test mice continued to drink the 851 nutrient solution.
CCI. at 4 p.m. was administered intraperitoneally. The mice in the control group were fed the same diet as the test group, except that they received tap water instead of 851NS and intraperitoneal administration of paraffin oil (CCI.solvent) on the 14th day.

C.C. Four control groups of mice were fed the same diet and allowed to drink tap water. The animals were fasted and the CCI<
(1.87 mmol/AIF) was administered intraperitoneally.

Animals were sacrificed on day 15 and liver samples were prepared. Ri
roshi Ohakawa (formerly roshi Obak)
Awa et at, ^nal. Biocl+em.
95:351-358. M[)8 in the liver was detected according to the method of 1979), and 1,1,3,3-tetrameth-1-xypropane (Japan TCI product, E. P. grade) was used as a standard sample for detection of lipid peroxide,
Absorbance at 80^ was detected using a spectrophotometer. MD^ content as nmo I per liver TiAg
Expressed as The data were analyzed statistically. The results were as follows.

As a result, it was found that 851 Eikan solution has strong anti-peroxidant lipid activity that is useful for anti-aging under certain conditions. Male Kuna+ing mice with a weight of 15 to 249 were used and divided into several groups. Mice were given water, ginseng, Nestle powdered milk and 851NS, respectively.

Mice in the 851NS group received 851NS (
5 to fhll days) for 4 days. Mice in the control group were given tap water for 4 days.

Mice in the Nestle powdered milk group were given 0.01% Nestle powdered milk instead of tap water for 4 days.

Cut natural Korean ginseng into slices, soak in boiling water for about 3 hours, then boil twice for 15 minutes while heating gently to give a concentration of 0.001%, and use this as a substitute for tap water for mice. I gave it for 4 days.

WaB Yun-muo (Journal or
Shanghai Medicine 2:46
-48. A swimming test was conducted according to the method of (1985).

The results are shown below.

As a result, it was found that the 851 nutrient solution significantly extended the swimming time of mice and improved the physique of mice.

After disposing of the used culture medium at the FCC, gastric cancer cells in a good breeding state (
FCC) was digested with 0.02% EDT^ and washed with 1640 medium containing 15% bovine serum. Viable cells were counted with tryptophan blue and total volume 1i+l (I
10'/l1) at 37°C for 24 hours. Cells were then washed and cultured in the same medium containing 851NS (0.2zffi/i1). control group cells to a volume of 1zf
fi medium (1640 medium containing 15% bovine serum 1
ose and 851NS2zi') at 37°C for 24 hours. Two wells were prepared for each group. The spent medium was then discarded. 0.02% EOTAQ. ! IfR
1 and 1・Reply 1・Fumble 10. Li& was added and viable cells were counted.

Suppression rate = Shi21 x 100% l1? and n2 are the number of viable cells in the control group and test group, respectively.

As a result, it was found that the average inhibition rate of 851NS against gastric cancer cells was 30%.

1. Euloteserassus Euplotes crassus is a unicellular organism that lives in seawater. Cell age is expressed by asexual generations and ranges from 1000 to 1500. Aging of Euplotes crassusi cells is associated with certain metabolic changes. Among these changes, a decrease in the rate of cell division is one of the most prominent features of cellular aging. The division rate of Euplotes crassus cells was significantly increased when the cells were cultured in seawater containing 10% 851NS. The cell division rate for 5 days during the culture period (7 days of generation) is shown below.

As is clear from the table above, Euplotes cras
sus [The cell division rate is 851NS with a seawater medium of 10%.
When containing 1. l1-l. It increased by 67 times. 851
When NS was phagocytosed into cells, cell metabolism was improved. When the medium contains 10% 851 nutrient solution, the division rate of aged Euplotes crassus cells (Zoo generation age) is 1.4 times (0.33-0.79 generations/
day) increased. The results showed that 851 nutrient solution can improve the metabolism of aging cells. 1. Materials: 1.
al College of Animal Center. 8
The batch number for the 51 nutrient solution is 06.

2. Method: I Two pregnant Kunming mice were fed standard chow and tap water separately. After parturition, 12 mice were randomly divided into control group and 851NS group. Newborn mice from the same litter (born from the same parents) were weighed at the time of suckling. Mothers and newborn mice of the control and 851NS groups were fed standard feed or feed containing 851NS during the suckling period and 15 days after weaning, respectively. Next, newborn mice from the same litter were weighed. Fluid samples from the eyeballs of newborn mice were collected.

The weight gain and white blood cell classification of newborn mice were as follows. Class 1 Self Lv'] Otsu l. Results: 1.1 Table 1 shows the changes in body weight of newborn mice. 1.2 Table 2 shows the effect of 851NS on leukocyte differentiation in newborn mice. Δ2 group: Gisa Keill ■ 2. Discussion 851 nutrient solution was a bioengineered product containing over 20 amino acids, several vitamins, and trace elements. As is evident from the results, 851 nutritional solution significantly increased the level of lymphocytes in newborn mice, thus it was found that 851NS could stimulate and improve the hematopoietic function of newborn mice.

Furthermore, the results revealed that 85R4S can increase the weight of newborn mice, and 851NS contains components that promote the growth of newborn mice.

Do you want to go to the bottom of the 4th L mouse? Se-ju ■
Vessel R1L 1. Materials: uI male Kunming mice weighing 42-44 g were obtained from the animal center of Fujian Medical College.

851NS, 4N IC+ and 0.1% indomethacin were used. 2. Method 2.1 Creation of subchronic injury model of gastric mucosa in mice Eight mice were randomly divided into two groups. Normal saline was orally administered to mice in the control group once a day. Mice in the test group received alternating doses of 48 HCI or 0.1% indomethacin.
The drug was administered (orally) once a day for 2 days. These mice were fed regular chow and tap water. After 10 days, the mice were sacrificed. The stomachs were removed and washed with water. The gastric mucosa of the control group showed a shiny red color, but the gastric mucosa of the test group was dull, pale, and swollen. The gastric cavity was filled with 10% neutral formalin for fixation, divided into sections with paraffin wax, and stained with IIE staining. Microscopic examination of the gastric mucosa revealed that the endothelium of the mucosa was thinned and covered with corroded keratinous material, and the gastric glands were colonized with corroded necrosis. No significant changes were observed in the control group. It was confirmed that the above method can serve as a model for subchronic injury.

2,2 851NS against subchronic injury of gastric mucosa in mice
Therapeutic effect of 30 mice were randomly divided into control group and 851 group, and HCI or indomethacin was administered alternately for 10 days according to the above method. These two groups of mice were given standard chow or IIIril food containing 851 for 10 sips, and even after the administration of HCI or indomethacin was stopped, the
Feed was continued for days. Then the mice were killed. Changes in gastric mucosa were tested according to the above method.

No significant difference was observed in the morphology of the gastric mucosa between the Hige-sai L1 color control and 851 groups. The anterior stomach mucosa of the 851 group was slightly thinner than that of the control group. As a result, 851 was found to be beneficial in the recovery of gastric mucosal damage.

1. Method Rats were randomly divided into control and 851 groups and fed standard chow and chow containing 851 (8i+ffi/rat), respectively. Provide all rats with sufficient tap water. After 15 days, all rats were fasted for 14 days and indomethacin (2.5i+y/rat) was subcutaneously administered once a day for 2 days. Eighteen hours after the second injection, the rats were sacrificed and the stomachs were examined.

Symptom length L {roasted l. Results Number and extent of acute bleeding in the gastric mucosa: The bleeding in the gastric mucosa of 6 mice in the control group and 4 mice in the 851 group was locally limited in a patchy, striped or elongated state. Control group 1
Diffuse hemorrhage of the gastric mucosa occurred in one rat. The number of bleeding points on the gastric mucosa was 6 to 12 in the control group and 2 to 8 in the 851 group. The number of gastric mucosal hemorrhages in the control group was significantly higher than in the 851 group.

2. As is clear from the results, gastric mucosal bleeding induced by indomethacin was prevented when rats were given 85INS for 15 days.

1. 22 l'listar rats weighing 140-22h
Fujian Me (10 males and 2 females)
Obtained from animal center of dical college. CCI. The analytical grade is refined peanut oil with a concentration of 40%.
851NS diluted in agar gel (Shangha
i Che+sical Agent! 4anufa
ctory products> and LEB versatile electrophoresis equipment. 2. Method 22 rats were randomly assigned to control group (n=6), CCI
．． The patients were divided into three groups: group (ii=8) and CCI4+851 group (low 8). CCI. and CCl. Rats in the +851 group received CCl. (100 g of 0.1 taki U) was administered subcutaneously twice a week (every 3 days) for 5 weeks. Control and C
C.I. The rats in the group were fed standard chow. CCI, +8
Group 51 rats were fed a diet containing 851NS (8zU
Rat. 7 days). All rats were provided with sufficient tap water and food for 5 weeks; 5 days after the final administration of CCl, blood was collected from the axillary vein; serum was collected and liver function was detected; using an LEB electrophoresis device. Electrophoresis of serum proteins was performed. Pathological changes in the liver were examined. Aya 4LL1 East 1. Results: 1.1 Rat SGPT CCI. Rat SGI'T and 851NS Kyo p1 was compared to the control group, p2 was CCI. Compare to groups. 1.
2 No significant differences were observed in the thymol turbidity test (TTT) and zinc sulfate turbidity test @ (ZnTT) between these groups. 1.3 Visual observation of the liver: The color and gloss of the liver sac in the control group were normal. The liver sac was shiny. CCI. The liver sacs of rats in the group showed watery degeneration.
The color of the liver was light brown and lackluster, in accordance with the morphological changes of the liver due to aging. CCI. The liver morphology of rats in the +851 group was CCI. It was better than the control group, but worse than the control group.

1.4 Liver observation under a microscope: In the control group, the liver structure of five rats except one with weak watery degeneration was normal, CCI. Eight rats in the group showed significant hepatic watery degeneration. Plasma in the lateral parts of the liver lobules showed diffuse or focal reticular areosis. There were normal cells and degenerated cells in the central part of the liver lobules. The steatotic hepatocytes were scattered or concentrated, and it was difficult to distinguish them from the watery degenerated cells. CCl.
The livers of 6 rats in the +851 group were treated with CCI. The other two rats had less watery degeneration than the rats in the CCI.
It showed the same degree of watery degeneration as the group. The liver histological changes in rats followed the results of liver function S(;PT. This means that the results of rat SGI'T in all groups were reliable.

1.5 Electrophoresis of rat serum proteins: Two serum samples from each group were used for electrophoresis. The results showed that there were no differences between these groups. From these results, CC
I. It was found that the liver injury induced by 20% did not result in changes in serum proteins in rats.

1,6 Weight gain: CCl. Although the weight gain of rats in the +851 group was greater than that of other groups, there were no statistically significant differences between these groups (p>0.05)
．． 2. Discussion As a result of visually and microscopically examining rat serum SGPT and morphological changes, it was found that 85l exhibited preventive and therapeutic effects on CCI-induced subchronic liver damage. 1. Materials Kunming mice were obtained from Fujian Animal Center. It was used. 851NS of Medical College (batch number 06) 2. Method: Twenty-seven Kunming female mice, which were mated with male mice of the same species and considered to be pre-pregnant (with vaginal thrombus), were randomly divided into a control group and an 851 group. Mice in the control group were fed standard chow, and mice in the 851 group were fed chow containing 851. All mice were given tap water. Seven mice were randomly selected from each group, and after confirming that they were pregnant due to enlarged abdomens, they were fed separately using the method described above. Newborn mice from the same litter were weighed on the day of birth and after suckling for 20 days. The weight and appearance of newborn and suckling mice were examined. Class 5 R914. l. Result 1. Effect of R1 on the body weight of newborn mice (immediately after birth)
Effects of 51NS Table 3: Effects of BoI on the body weight of newborn mice compared with the main control group. 1.2 The effect of 851NS on the body weight of mice during the suckling period
Shown in the table.

1.3 No abnormalities were observed in appearance, eating ability, or general activity. 2. Discussion 2.1 As is clear from Table 3, when 851NS was given to female mice during pregnancy, 851NS was able to promote weight gain in mouse fetuses (p<0.01).

2.2 As is clear from Table 4, when 851NS was given to female mice during the lactation period, 851NS was able to promote the weight gain of the suckling mice <p<0.05
).

2.3 As is clear from the results, when female mice were given 851NS for 40 days from pregnancy to lactation, abnormalities occurred (
For example, no fetal malformations or adverse effects were observed. Examples of the present invention will be described below in a non-limiting manner. Oito-tsuru L A 2.5 t capacity tank fermenter was used for this preparation.

The seed fermenter had a capacity of 0.5 t. The raw material charge is 40
%, soybean powder 5%, yeast extract 0.04%, K
ll2P0. 0.05%, MgSO. 0.02%,
CaCOs 0.02%, NaClO. 02%, Na
2MoOi 10ppm, Na2SeO. 2.5p
p(ZnSO. 10ppm, CoCl22.5ppm
A 5% soybean medium was used as a production medium containing water and water. The amount of inoculum is 1%, the seed fermenter is 21,
Cell age is 24 hours (1st seed culture - 2nd seed culture - 3rd seed culture)
Seed culture, culture incubated for 24 hours was used as inoculum), culture temperature was 30 °C, stirring speed was 26
0rpse, aeration rate 1:0. It was called aVV seagull. Incubation of the culture lasted 45 hours. After incubation, the culture was autoclaved, passed through an assembly line into bottles, and then autoclaved again to give the final product. Culture solution 100z1 contained 3.7% dry material, 2.06% protein, 0.23% lipid, 0.75% carbohydrate, vitamins and inorganic salts (Table 5). An 8 ton tank fermenter was used for this preparation. The amount of raw material charged was 40%. In other words, the amount of medium was 3.2t.
It was. 10% soy milk was used. Medium is soybean 32
0 &, yeast extract 1. 28k9, κH2PO. i
．． e. fI,? 8SO4 640y- CaCOs
640g, NaCI 6401I, NI12MO
04321F, NazSeO* 8g, ZnSO+
32y, 8g of CoCl and water.
The amount of inoculum was 1%, and the cell age was 24 hours (first seed culture, second seed culture, and third seed culture; cultures incubated for 24 hours were used as inoculum). Culture temperature 30°C. Stirring speed 220 rpm, aeration rate 1:0
．． It was set to 8vvm. After incubation in the seed fermenter, the inoculum was inoculated into an 81-container fermenter, and the culture temperature was 30°C, the stirring rate was 22Grp, and the aeration rate was 1:0.8.
vvm. After 48 hours, the incubation was terminated, and the culture solution was autoclaved, passed through an assembly line, bottled, and autoclaved again to obtain the final product. Culture solution 1
00jf is asparachynic acid 543mg, glu9mine vi710mg, serine 160B, glycine 280mg
, histidine 65mg, arginine 226u, threonine 232JllF, alanine 359ig, broline 12
6+B, tyrosine 10fB, valine 283i+g, methionine 53, isoleucine 262B, leucine 309
1Il? , phenylalanine 148JIIF, lysine 2
It contained 12B, cystine 38 and 9, totaling 4172 zg.

The nutrient solution prepared by the method described in Example 2 from Oito Bonito was dried using a spray dryer commonly used in the production of dried milk to obtain 851 nutrient powder. The inlet temperature of the spray dryer was 80°C, and the outlet temperature was 60°C. 851 Nutrient Solution 0.81 to Dry Powder 20.4 AFI
This was further processed and made into capsules. One capsule contained 0.28 g of dry powder, or was formulated into a granule preparation with 80A starch and 10A9 honey. 851 nutrient solution prepared by conventional fermentation method to 150
Centrifugation was performed at 0 rpm to obtain a precipitate. Thus, 85
960 kg of supernatant was obtained from 1 ton of nutrient solution. fruit juice 500
851 fruit juice cola was prepared by adding &g, and 851 honey cola was prepared by adding 40 kg of honey. Flavoring agents can also be added to the supernatant to prepare various 851 beverages with different tastes as desired.

851 Nutrient solution prepared by conventional fermentation methods was centrifuged. The supernatant was concentrated by membrane evaporator or vacuum rotary evaporator to obtain 851 concentrated nutrients. After evaporation, concentrated nutrients 100A9 were obtained from 0.5 L of supernatant. After adding the honey toe, the concentrated nutrients were filled into vials with easy-open caps. This nutrient is convenient to take orally.

Claims (9)

[Claims] (1) It has the ability to convert plant proteins, especially soybean proteins, into single-cell proteins, is a Gram-positive bacterium, is non-motile, is capable of producing H_2S, and is nitrate reduction positive; It survives in milk when heated to 60°C for 30 minutes or 72°C for 15 minutes, has red or orange-red thallus, and the color of the thallus changes to pink at 38°C and colorless at 40°C, indicating that the strain Microbacterium mutant strain 851R whose DNA is characterized by containing an R fragment detectable by a specific R fragment DNA probe. (2) 12301 Parklawn Drive, USA
e, Rockville, Marylad 20852
American Type Culture located in
Collection includes A. T. C. C. Deposit number 53
Microbacterium mutant strain 851R having essential characteristics of the sample deposited in 981. (3) A method for producing a single-cell protein nutrient solution by using bacteria, using Microbacterium mutant strain 851R as a production strain and as a production medium for producing 851R nutrient solution by fermentation. A method characterized by using a soybean powder medium or a starch medium. (4) The method according to claim 3, characterized in that an oven-drying spray process is used to collect single cell proteins from the culture solution, and a concentration process is used to purify the culture solution. (5) 5-10% by weight of soybean powder or 5-20% by weight of soybean milk (calculated as soybean weight) or 5-10% by weight of various pulse cakes, sunflower seed cakes, yeast extract or yeast powder 0.02-0. 5% by weight, CaCO_30.0
5-0.5% by weight, K_2HPO_40.02-0.2
Weight %, MgSO_40.01-0.08 weight %, Na
Cl0.01-0.08% by weight, Na_2MoO_45
-50 ppm by weight, Na_2SeO_31.5-10 ppm by weight, ZnSO_42.5-50 ppm by weight and C
Medium of strain 851R according to claim 1, containing oCl_22.5-50 ppm by weight. (6) Raw materials containing starch (sweet potatoes, potatoes, etc.)
10-20% by weight or 1-10% by weight of starch-containing dry materials (such as corn flour), yeast extract 0.04-0.
08% by weight, CaCO_30.05-0.5% by weight, K
_2HPO_40.05-0.1% by weight, MgSO_4
0.02-0.04% by weight, NaCl0.02-0.0
4% by weight, Na_2MoO_410-20 ppm by weight,
Na_2SeO_31.5-20 ppm by weight, ZnSO
Medium of strain 851R according to claim 1, containing _45-20 ppm by weight and CoCl_25-20 ppm by weight. (7) Nutrient solution produced by fermentation with Microbacterium mutant strain 851R in a medium containing a nitrogen source. (8) The solution has an anti-aging effect, a constitutional improving effect, a cancer cell proliferation suppressing effect, an effect of stimulating and improving blood formation in living organisms, an effect of recovering gastric mucosal damage, a therapeutic effect on acute bleeding in the gastric mucosa, a therapeutic effect on liver damage, and a postpartum constitution. 851R nutrient solution according to claim 7, characterized in that it has a restorative effect of. (9) A series of products manufactured from bacteria of strain 851R or from 851 nutrient solution.