JPH0360064B2 - - Google Patents
Info
- Publication number
- JPH0360064B2 JPH0360064B2 JP4734684A JP4734684A JPH0360064B2 JP H0360064 B2 JPH0360064 B2 JP H0360064B2 JP 4734684 A JP4734684 A JP 4734684A JP 4734684 A JP4734684 A JP 4734684A JP H0360064 B2 JPH0360064 B2 JP H0360064B2
- Authority
- JP
- Japan
- Prior art keywords
- serum
- ion concentration
- blood
- value
- aminomethane
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 210000002966 serum Anatomy 0.000 claims description 18
- 210000004369 blood Anatomy 0.000 claims description 13
- 239000008280 blood Substances 0.000 claims description 13
- 239000007983 Tris buffer Substances 0.000 claims description 11
- 239000000872 buffer Substances 0.000 claims description 11
- 238000000034 method Methods 0.000 claims description 11
- 238000002203 pretreatment Methods 0.000 claims description 3
- 150000002500 ions Chemical class 0.000 description 24
- 239000011575 calcium Substances 0.000 description 22
- 238000001727 in vivo Methods 0.000 description 5
- 238000005259 measurement Methods 0.000 description 5
- 238000010790 dilution Methods 0.000 description 3
- 239000012895 dilution Substances 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
- 239000000654 additive Substances 0.000 description 2
- 238000007796 conventional method Methods 0.000 description 2
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 2
- BHPQYMZQTOCNFJ-UHFFFAOYSA-N Calcium cation Chemical compound [Ca+2] BHPQYMZQTOCNFJ-UHFFFAOYSA-N 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 238000007664 blowing Methods 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 229910001424 calcium ion Inorganic materials 0.000 description 1
- 229910002092 carbon dioxide Inorganic materials 0.000 description 1
- 239000001569 carbon dioxide Substances 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 125000004029 hydroxymethyl group Chemical group [H]OC([H])([H])* 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- NQMRYBIKMRVZLB-UHFFFAOYSA-N methylamine hydrochloride Chemical compound [Cl-].[NH3+]C NQMRYBIKMRVZLB-UHFFFAOYSA-N 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000007711 solidification Methods 0.000 description 1
- 230000008023 solidification Effects 0.000 description 1
- 239000012086 standard solution Substances 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/84—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving inorganic compounds or pH
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Immunology (AREA)
- Urology & Nephrology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Hematology (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Biotechnology (AREA)
- Inorganic Chemistry (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Description
【発明の詳細な説明】
本発明は、好気的条件下におかれた血清を検体
としそれに前処理を施こすことによつて、生体内
における血液のカルシウムイオン（以下、Ca2+
イオンという。）濃度を測定できるようにした血
液中のCa2+イオン濃度測定のための前処理方法
に関する。Detailed Description of the Invention The present invention uses blood serum under aerobic conditions as a sample and pre-processes it to detect blood calcium ions (hereinafter referred to as Ca 2+
It's called ion. ) A pretreatment method for measuring Ca 2+ ion concentration in blood that enables the concentration to be measured.
Ca2+イオンはH+と蛋白との結合において競合
しており、採血後の検体が空気に触れると炭酸ガ
スが脱気してPH値が上昇し、その結果Ca2+イオ
ンが減少する。従つて、好気的条件下におかれた
血清をそのまま検体として用いて測定したので
は、生体内におけるCa2+イオン濃度を得ること
ができない。このため従来は、全血状態で測定す
る方法とか、好気的条件下にある血清にCO2ガス
を吹き込むという前処理を行なつて、PH値を生体
内の血液中におけると略同じ7.4付近に戻してか
ら測定する方法が用いられていた。しかるに前者
の方法であると、血漿分離がなされていないの
で、素早く測定を行なわないと固まるおそれがあ
り、そのため多数の検体を測定する場合には不向
きでほとんど実用性がないといえる。一方、後者
の方法は血清中のCa2+イオン濃度がPH値に依存
していることを利用したもので、前者におけるよ
うな欠点もなく、比較的正確に生体内の血液中に
含まれるCa2+イオン濃度を得ることができるの
であるが、反面CO2ガスの吹き込みに時間がかか
るし、CO2ガスを発生し、血清に吹込ませるため
の装置が非常に大型化するといつた難点がある。 Ca 2+ ions compete with H + in binding with proteins, and when a sample after blood collection is exposed to air, carbon dioxide gas is degassed and the PH value increases, resulting in a decrease in Ca 2+ ions. Therefore, it is not possible to obtain the Ca 2+ ion concentration in the living body by measuring serum that has been placed under aerobic conditions as it is as a specimen. For this reason, conventional methods have been used to measure the pH value in whole blood, or pre-process by blowing CO 2 gas into the serum under aerobic conditions, so that the PH value is around 7.4, which is approximately the same as in blood in vivo. A method was used in which measurements were taken after the temperature was returned to . However, in the former method, since plasma separation is not performed, there is a risk of solidification unless the measurement is carried out quickly, and therefore it is unsuitable when measuring a large number of specimens and is almost impractical. On the other hand, the latter method takes advantage of the fact that the Ca 2+ ion concentration in serum depends on the PH value, and does not have the drawbacks of the former, and relatively accurately measures the Ca 2+ ion concentration in blood in vivo. 2+ ion concentration can be obtained, but on the other hand, it takes time to inject CO 2 gas, and the disadvantage is that the equipment for generating CO 2 gas and injecting it into the serum becomes very large. .
本発明者は、好気的条件下にある血清のPH値を
生体内の血液における値まで戻す手法として、バ
ツフアを血液に添加するという新たな手法を着想
した。しかし、バツフアとして用いることのでき
る添加剤はPH値を下げる効果はあつても、Ca2+
イオンと結合するものが大半で、そのためCa2+
イオン濃度を生体内の値まで戻すことはできない
ものであつた。そこで本発明者は多くの添加剤を
個別に、或いは混合等して試験を行なつた結果、
Ca2+イオンと結合の少ないバツフアを現出させ
たのである。 The present inventor conceived of a new method of adding buffer to blood as a method of returning the PH value of serum under aerobic conditions to the value of blood in vivo. However, although additives that can be used as buffers have the effect of lowering the PH value, they
Most of them combine with ions, so Ca 2+
It was not possible to return the ion concentration to the in-vivo value. Therefore, the present inventor conducted tests using many additives individually or by mixing them, and found that
This resulted in the appearance of a buffer with less binding to Ca 2+ ions.
本発明は、このような本発明者の着想及びその
後の試験・研究によつて完成されたもので、従来
手法におけるような欠点のない新規かつ有用な手
法を提供することを目的としている。 The present invention was completed based on the inventor's ideas and subsequent tests and research, and aims to provide a new and useful method that does not have the drawbacks of conventional methods.
而して、本発明に係る血液中のCa2+イオン濃
度測定のための前処理方法は、好気的条件下にあ
る血清にバツフアとしてトリス（ヒドロオキシメ
チル）アミノメタン−HClを加えて血清中のCa2+
イオンを測定することを要旨とする。 Therefore, in the pretreatment method for measuring Ca 2+ ion concentration in blood according to the present invention, tris(hydroxymethyl)aminomethane-HCl is added as a buffer to serum under aerobic conditions. Ca 2+ in
The gist is to measure ions.
トリス（ヒドロオキシメチル）アミノメタン−
HClは添付図のバツフア濃度−Ca2+イオン濃度曲
線に示すようにトリス（ヒドロオキシメチル）ア
ミノメタン−HCl濃度を増やしていつても検体の
Ca2+イオン濃度が変化しないという特性がある。
このことはトリス（ヒドロオキシメチル）アミノ
メタン−HClがCa2+イオンとほとんど結合しない
ことを意味する。従つて、トリス（ヒドロオキシ
メチル）アミノメタン−HClをバツフアとして用
い、好気的条件下にある血清のPH値を生体内の血
液のPH値付近に戻して測定すれば、生体内の
Ca2+イオン濃度を得ることができるのである。
このように本発明方法によれば、好気的条件下
にある血清を検体として用いることができるの
で、血清の保存に留意する必要がない。血清に
バツフアを加えるだけで測定できるので、前処理
装置として大掛かりな装置が不要である。 Tris(hydroxymethyl)aminomethane-
As shown in the buffer concentration - Ca 2+ ion concentration curve in the attached figure, HCl can be used to increase the concentration of tris(hydroxymethyl)aminomethane-HCl in the sample.
It has the characteristic that the Ca 2+ ion concentration does not change.
This means that tris(hydroxymethyl)aminomethane-HCl hardly binds to Ca 2+ ions. Therefore, if you use tris(hydroxymethyl)aminomethane-HCl as a buffer to return the PH value of serum under aerobic conditions to around the PH value of blood in the body and measure it, the PH value of the blood in the body can be measured.
This allows us to obtain the Ca 2+ ion concentration.
As described above, according to the method of the present invention, serum under aerobic conditions can be used as a specimen, so there is no need to be careful about preserving serum. Since measurements can be made by simply adding buffer to serum, no large-scale pretreatment equipment is required.
といつた効果がある。尚、図中の曲線Ａ及びＢ
は一定のCa2+イオンを含む溶液においてバツフ
ア濃度を変化させたときのCa2+イオン濃度を実
測した値である。 There is a certain effect. In addition, curves A and B in the figure
is the actually measured value of Ca 2+ ion concentration when the buffer concentration is changed in a solution containing constant Ca 2+ ions.
次に本発明の実施例を説明する。 Next, embodiments of the present invention will be described.
実施例 １
試検管等にトリス（ヒドロオキシメチル）アミ
ノメタン−HCl（PH7.0〜7.4）の高濃度溶液を入
れ、蒸発乾燥させる。これに一定量の血清を入れ
撹拌して、固化したトリス（ヒドロオキシメチ
ル）アミノメタン−HClを溶解させる。これを検
体としてCa2+イオン濃度測定装置にてCa2+イオ
ン濃度を測定する。Example 1 A highly concentrated solution of tris(hydroxymethyl)aminomethane-HCl (PH7.0-7.4) is placed in a test tube and evaporated to dryness. Add a certain amount of serum to this and stir to dissolve the solidified tris(hydroxymethyl)aminomethane-HCl. Using this as a sample, the Ca 2+ ion concentration is measured using a Ca 2+ ion concentration measuring device.
ここで、トリス（ヒドロオキシメチル）アミノ
メタン−HClを乾燥させて用いるのは、溶液であ
ると血清のCa2+イオン濃度が薄くなり、正確な
測定が行なえなくなるおそれがあるためである。
また、加える血清の量はバツフアの終濃度が50〜
200ｍmol／となるような適当量とすればよい。 Here, the reason why tris(hydroxymethyl)aminomethane-HCl is used after drying is that if it is in solution, the concentration of Ca 2+ ions in serum may become diluted, making it impossible to perform accurate measurements.
In addition, the amount of serum to be added is such that the final concentration of serum is 50~
The amount may be set to an appropriate amount of 200 mmol/.
実施例 ２
オートサンプラーで血清中のCa2+イオン濃度
を測定する場合には、サンプル（血清）の流れる
ラインに一定量のトリス（ヒドロオキシメチル）
アミノメタン−HClを加えて測定する。このとき
のトリス（ヒドロオキシメチル）アミノメタン−
HClは、水溶液状のものを用いる。その濃度とし
て１〜2mol／の高濃度のものを用い検体の希
釈が1.1倍以上にならないように少量のバツフア
を注入する。しかしこのままでは検体の希釈が無
視できないので校正用標準液がシステムを流れる
ときも同様に本バツフア溶液を加え、希釈分をキ
ヤンセルして測定する。この方法をとれば機器の
差等により注入量の絶対値が変化しても欲しい生
体内Ca2+イオン濃度値が得られる。Example 2 When measuring the Ca 2+ ion concentration in serum with an autosampler, a certain amount of Tris (hydroxymethyl) is added to the sample (serum) flow line.
Measure by adding aminomethane-HCl. At this time, tris(hydroxymethyl)aminomethane-
An aqueous solution of HCl is used. A high concentration of 1 to 2 mol/ml is used, and a small amount of buffer is injected so that the dilution of the specimen does not exceed 1.1 times. However, as it is, the dilution of the sample cannot be ignored, so when the calibration standard solution flows through the system, the buffer solution is added in the same way to cancel the dilution and perform measurements. By using this method, the desired in-vivo Ca 2+ ion concentration value can be obtained even if the absolute value of the injection amount changes due to differences in equipment.
図はバツフア濃度−Ca2+濃度曲線を示す実測
データである。
The figure shows actually measured data showing a buffer concentration-Ca 2+ concentration curve.
Claims (1)
リス（ヒドロオキシメチル）アミノメタン−HCl
を加えてCa2+イオンを測定することを特徴とす
る血液中のCa2+イオン濃度測定のための前処理
方法。1 Add tris(hydroxymethyl)aminomethane-HCl as a buffer to serum under aerobic conditions.
1. A pretreatment method for measuring Ca 2+ ion concentration in blood, the method comprising: measuring Ca 2+ ion concentration in blood.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP4734684A JPS60190865A (en) | 1984-03-12 | 1984-03-12 | Pretreatment for measuring concentration of ca2+ ion in blood |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP4734684A JPS60190865A (en) | 1984-03-12 | 1984-03-12 | Pretreatment for measuring concentration of ca2+ ion in blood |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS60190865A JPS60190865A (en) | 1985-09-28 |
| JPH0360064B2 true JPH0360064B2 (en) | 1991-09-12 |
Family
ID=12772591
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP4734684A Granted JPS60190865A (en) | 1984-03-12 | 1984-03-12 | Pretreatment for measuring concentration of ca2+ ion in blood |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS60190865A (en) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10087612B2 (en) | 2014-02-12 | 2018-10-02 | Fresh Products, Inc. | Floor shield |
| USD778411S1 (en) | 2014-11-05 | 2017-02-07 | Fresh Products, Inc. | Urinal screen |
| DE202015102279U1 (en) | 2014-11-05 | 2015-08-14 | Fresh Products, Inc. | urinal |
| CA3084992A1 (en) | 2017-12-20 | 2019-06-27 | Fresh Products, Inc. | Urinal screens |
| USD915786S1 (en) | 2018-08-31 | 2021-04-13 | Fresh Products, Inc. | Absorbent mat |
| USD925009S1 (en) | 2018-10-25 | 2021-07-13 | Fresh Products, Inc. | Urinal screen |
| USD1078945S1 (en) | 2022-07-13 | 2025-06-10 | Fresh Products, Inc. | Urinal screen |
-
1984
- 1984-03-12 JP JP4734684A patent/JPS60190865A/en active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| JPS60190865A (en) | 1985-09-28 |
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