JPH0361428A - Method for culturing row wood log for mushroom - Google Patents

Abstract

PURPOSE:To efficiently enable to inoculate in a short period by inserting tips of a chip spawn into an inoculation hole previously formed, aslant moving the chip spawn in a state nearly coinciding a breaking ditch formed in the chip spawn with the opening part of inoculation hole and breaking off the part inserted into a raw wood log from the breaking ditch and inoculating to the part. CONSTITUTION:Breaking ditch 2 axially and perpendicularly crossing at prescribed intervals is formed in a relatively long rod-like shaped article obtained by heating and forming a raw material in which nutritive elements such as row wood or rice bran necessary for the growth of mushroom hypha are added to a synthetic resin harmless to the proliferation of hypha of mushroom of provide a chip spawn raw material 1a. Spawn of mushroom is cultured in the chip spawn raw material 1a to give a chip spawn 1. The tip of the chip spawn 1 is inserted into a spawn hole 5 and the chip spawn 1 is aslant moved in a state nearly coinciding a breaking ditch 2 formed in the chip spawn with the opening of spawning hole 5 and broken off. Thus, the chip spawn 1 is successively spawned into a spawning hole 5. Thereby the spawning of chip spawn 1 to row wood log 4 can be simply and readily carried out and, simultaneously, the time necessary for the spawning can be remarkably shortened.

Description

[Detailed Description of the Invention] [Industrial Application Field] The present invention relates to a method for cultivating mushroom logs using so-called seed pieces.

[Conventional technology]

There are two methods for cultivating mushroom logs: a sawdust starter, which is made by culturing mushroom fungi on sawdust containing nutrients such as rice bran, and a seed starter, which uses seeds made by culturing mushroom fungi on small pieces of wood of various shapes. There is a way.

Mushroom cultivation using seed fungi is one of the methods.

Simply drive the seed piece (the seed piece material with mushroom fungi cultured; the same shall apply hereinafter) into the inoculation hole formed in the log.
It is easier to inoculate than sawdust starter, and is mainly used for cultivating shiitake mushrooms and nameko mushrooms.

In other words, in the conventional cultivation using seed pieces, a cylindrical or truncated cone-shaped seed material with a length of about 18 mm is placed in a culture bottle, two types of seed material are sterilized, and then, for example, Shiitake fungus is added to the seed material. is inoculated and cultured in the dark in a culture room until the Shiitake bacteria are sufficiently spread in the seed material stored in a culture bottle.

Therefore, when cultivating, the producer first removes the packed seeds from the packaging bag and uses an appropriate device to remove excess bacteria that have adhered to the surface of the seeds. After that, seed pieces were driven into the inoculation holes formed in the log using an electric drill, etc. one by one using a hammer, etc. After that, the log with the second type seed placed in was placed face down according to the usual method, and the shiitake mushrooms were grown. Cultivated.

[Problem to be solved by the invention]

Cultivating mushrooms on logs using seed pieces requires the work of driving the first seed pieces one by one into inoculation holes formed in the log with a hammer or the like when inoculating the logs.

This driving operation is extremely troublesome for the two workers as they hold relatively small seed pieces (as mentioned above, diameter 1 ON, length 18 mm) with their fingers and drive them into the inoculation hole, which can result in injury. There are many troubles such as doing something wrong or damaging the 2nd type piece.

In addition, this driving requires repeated work of driving the seedlings one after another into a large number of seeding holes formed in the raw wood, so there is a problem in that the two workers are highly fatigued and one job takes a long time.

In view of the current situation, it is an object of the present invention to provide a method for cultivating mushroom logs that allows for simple and easy inoculation of seeds onto logs.

[Means to solve the problem]

In order to achieve this purpose, the method for cultivating mushroom logs of the present invention involves heating a raw material in which nutrients necessary for growing mushrooms, such as sawdust and rice bran, are added to synthetic resin powder that is not harmful to the growth of mushroom mycelium. A relatively long rod-shaped molded product obtained by molding was used as a seed piece material by forming fold grooves perpendicular to the axial direction at a predetermined interval, and mushroom seed bacteria were cultured on this seed piece material. Using a seed piece, insert the tip of the seed piece into the inoculation hole formed in the log, and insert the seed piece with the crease formed on the second seed piece aligned with the opening of the inoculation hole. It is characterized by sequentially inoculating seed pieces into the inoculation holes by tilting and breaking them off from the crease.

The seeds used in this invention are sawdust.

The raw material is a mixture of rice bran, corn bran 8 gypsum, and other nutrients that promote the elongation and growth of mushroom mycelia, and a synthetic resin that binds these nutrients and solidifies them into a predetermined shape, and this raw material is made into a mold. It is a relatively long piece that is injected and heated and molded into a rod shape.

The synthetic resins used are thermosetting resins such as epoxy resins, as well as thermoplastic resins such as polypropylene, high-density polyethylene, and vinyl chloride resins, which do not inhibit the growth of mushrooms.

Hereinafter, a method for producing round rod-shaped seed pieces used in the present invention will be explained based on the accompanying drawings.

As shown in FIG. 1, the seed piece 1 used in the present invention has a relatively long round bar shape as a whole, and has fold grooves 2, 2, 2, 2, 2, 2, 2, 2, 2, 2, 2, 2, 2, 2, 2, 2, 2, 2, 2, 2, 3, 3, 3, 3, 3, 3, 3, 3, 3 and 4 being at a certain intervals are each extend perpendicularly to the axial direction, ... is formed, and by cutting from this fold groove 2, the same individual seed pieces as before are made.

To make a long seed piece 1, add 20% by weight of epoxy resin powder to 80% by weight of nutrients such as sawdust, rice bran, etc. for elongation and proliferation of mushroom hyphae, stir well, and pour the mixture into a mold. , By heating, it is molded into a round bar shape with a diameter of about 8 to 10 mm and a length of about 20 to 50 cm. Mushroom bacteria are cultured on the material 1a by a predetermined method.

When molding the seed material, the amount of synthetic resin blended with respect to the nutrients is in the range of 5 to 20% by weight. It is undesirable because it tends to have poor rooting and proliferation.

In addition, the length of the 2nd type piece material depends on the workability of inoculating the raw wood and 1
It is calculated based on how many seed pieces are obtained from the book seed piece materials.

Note that this seed piece (2) is made by arranging a plurality of round rod-shaped seed piece materials 1a in parallel in a plane as shown in FIG.

Adjacent seed piece materials 1a, la... are mutually separably connected and integrated to form a plate-like body, and various piece materials la, Ia... on one side of the plate-like body are formed.・・・
Mushroom fungi are cultured on the plate-shaped body formed by forming fold grooves 2a, 2a, etc. by providing notches perpendicular to the axial direction at predetermined intervals, respectively. You may use it by separating it into seed pieces 1.

To cultivate the mushroom fungus, nine seed pieces 1a are immersed in water to absorb water to a moisture content of approximately 30% to 60%, and then multiple seed pieces 1a are soaked as shown in Figure 4. , la...
... is placed in a polyethylene culture bag 3, a large number of seed material 1a are sterilized together with the culture bag 3, and then a liquid mushroom seed is inoculated, the opening of the culture bag 3 is sealed, and the process is carried out in a culture chamber. be.

[For production]

The mushroom cultivation method of the present invention is carried out using a relatively long seed piece 1 in which grooves 2 are formed at predetermined intervals along the axial direction, as shown in FIG. Insert the tip of the second type piece 1 into the inoculation holes 5, 5... formed in the log 4,
If you tilt the seed piece (in the direction of the arrow) with the opening of the inoculation hole 5 and the crease 2 aligned, the tip will be separated from the crease 2, and the seed piece will be inserted into the log as in the previous method. It is something that can be inoculated into.

Therefore, the time required for inoculation can be significantly shortened compared to the conventional cultivation method in which individual seed pieces are driven one by one into the inoculation hole using a hammer or the like.

〔Example〕

The present invention will be explained in more detail below using Examples, Comparative Examples, and Reference Examples.

Loss of fertility on the brain 65% oak sawdust (wt%; same below).

A raw material was obtained by adding 15% of epoxy resin powder to 20% of nutrients consisting of rice bran and gypsum and stirring for about 30 minutes.

This raw material is molded into a mold consisting of an upper mold and a lower mold, in which 15 round bar-shaped seed pieces of 8 diameters x 230Ql in length can be molded into plate-like seed pieces that are separably connected to each other. Feed 150g into the mold, temperature 150℃, pressure 60kg/
It was heated and pressed for 5 minutes at J or above.

After cooling the obtained seed piece material of the plate-shaped body, 1 to 13 seed pieces (13 pieces x 15 pieces) are placed on each round rod-shaped molded article (seed piece material) on one side of the plate-shaped body. = 195 seed pieces) were made in the axial direction at intervals of 17.5 fl, and the diameter was W' 1 /
The grooves were formed at a depth of 2.

5 pieces of seed piece material (19
5 x 5 = 975 seeds can be collected).

After absorbing water to a level of about 40%, it is made of polyethylene and opened at the top.

The cells were placed in a culture bag with a sterilization filter made of nonwoven fabric attached to the ventilation hole formed near the opening.

Prepare 10 culture bags containing plate-shaped seed material, heat each bag until it reaches a temperature of 120°C, continue heating for 1 hour to sterilize it, and then store it at room temperature (approximately 20°C). "C)
cooled down to.

Next, Shiitake mushrooms were inoculated by spraying from the opening of the culture bag toward the seed material of the plate-shaped body, and then the opening of the culture bag was heat-sealed.

These were brought into a culture room and cultured in the dark at a temperature of 23° C. and a humidity of 70%, and the number of days required for the Shiitake fungus to spread and germination were investigated.

975 wooden seed pieces obtained using the traditional method were made to absorb water.
After adding nutrients, they were placed in pieces into the same culture bag as used in Example 1, inoculated with Shiitake mushroom under the same conditions, cultured in the dark, and the number of days of spread and bacterial growth were investigated.

The results are shown in Table 1 for comparison and comparison with the results of Example 1.

[Margin below]

Table 2 Next, the plate-shaped seed pieces obtained by the above method were separated from the mutually adjacent parts to obtain a total of 15 round rod-shaped seed pieces, and these were divided into 40 logs (with a diameter of about IQ). cm x length 90cm
The seeds were inoculated into 25 equally divided holes with a diameter of 9.2*m using an electric drill, and the time required to inoculate a total of 975 seeds was investigated.

The method of inoculation is as shown in Fig. 1, by inserting a round rod-shaped seed piece L into the seed hole 5 of about 9.2 wm in diameter and about 20 to 25 mm in depth formed in the log 4, and inserting it into the nuclide piece L. Formed crease groove 2
With the seed piece 1 aligned with the opening of the inoculation hole 5, the seed piece 1 was tilted in the direction of the arrow and cut using the folding groove 2.

On the other hand, the 975 seed pieces that had been cultured in Comparative Example 1 were inoculated using the conventional method (inserting the seed pieces one by one into the hole in the log and tapping their heads with a hammer to embed them), and the time taken to inoculate them was investigated. did.

The results are shown in Table 2.

Table 2 As is clear from Tables 1 and 2 above, the seeds used in this invention allow mushroom fungi to be cultured in an extremely short period of time, and inoculation onto logs is faster than before. This can be done in about 172 hours compared to seed inoculation.

Affected branch group A seed piece used in this invention made according to Example 1 (hereinafter referred to as this seed piece), a conventional wooden seed piece obtained according to Comparative Example 1 (hereinafter referred to as wood piece piece), and a conventional method Regarding the three types of seed pieces (hereinafter referred to as plaster pieces), which are made of plaster pieces with a bottom shape.

Shiitake mushrooms were cultivated by changing the diameter of seed pieces, the size of the logs to be inoculated, the cultivation location, etc., and the development of fruiting bodies was tested.

When inoculating the logs of various pieces, it is necessary to use one seed piece.

By inserting it into the inoculation hole and breaking it off through the crease,
The wood pieces and plaster pieces were inserted into the inoculation holes using a hammer or the like as before.

The results are shown in Table 3. Details in the item columns in the table are as follows.

1) A large-diameter tree, the average diameter of the log is 12-.

Small and medium seed trees are logs with an average diameter of 5-8 cm.

Furthermore, the broad-leaved forest is a Quercus oak forest.

2) Among the types of seed pieces, A represents a seed piece with a diameter of 8 m, and B represents a seed piece with a diameter of 10 meters.

3) Weight is the weight (g) of the fruiting body generated per 4f tree volume 0.1rr.

4) What is incidence rate?

The ratio (%) of shiitake mushrooms with a cap diameter of 4.5 cm or more to the total yield at. Mushroom inoculum is added to the seed material, which is made from a rod-shaped molded material obtained by adding nutrients necessary for growing mushrooms, such as sawdust and rice bran, to the powder.Fold grooves perpendicular to the axial direction are formed at predetermined intervals. The seed piece is cultured and used as a seed piece, and the tip of the seed piece is inserted into a pre-formed inoculation hole in the log, and the crease formed on the first seed piece is aligned with the opening of the seed piece. The seed piece is tilted and the part inserted into the log is broken off from the crease and inoculated.

Therefore, inoculation can be carried out by an extremely simple and easy method of inserting the tip of a relatively long seed piece into an inoculation hole formed in a log and breaking it off, and it can be done in the same way as conventional inoculation. Driving the seeds with a hammer etc.,
Since there is no need for sealing wax after inoculation, inoculation can be carried out efficiently in a short period of time.

On the other hand, the seed pieces used are relatively long and can be obtained simply and easily using a bottom mold, and the cultivation of the mushroom fungus can be completed in an extremely short period of time, and the germination state of the mycelia is good. Since it is a type of mushroom, the yield of mushrooms after inoculation is also high.

Moreover, it has the advantage of being able to produce products of good quality.

[Brief explanation of drawings]

FIG. 1 is a perspective view showing how seed pieces are inoculated into logs by the method of the present invention, FIG. 2 is a perspective view showing an example of seed pieces used in this invention, and FIG. 3 is a perspective view of seed pieces used in this invention. FIG. 4 is a perspective view showing an example of the culture state of the seeds used in the present invention. 1... Seed piece 1a... Seed piece material 2.2a...
Folding groove 3...Culture bag 4...Log 5...Inoculation hole

Claims (1)

[Claims] (1) Synthetic resin powder that is harmless to the growth of mushroom mycelia,
Crease grooves perpendicular to the axial direction at predetermined intervals are formed on a relatively long rod-shaped product obtained by heating and molding raw materials to which nutrients necessary for growing mushrooms, such as sawdust and rice bran, are added. A seed piece is used as a seed piece material, and a mushroom seed piece is cultured in this seed piece material, and the tip of the seed piece is inserted into the seed hole formed in the log, and the crevice formed in the seed piece and the seed piece are inserted. A method for cultivating mushrooms, which is characterized in that the seed pieces are tilted so that the openings of the fungus pores are aligned with each other, and the seed pieces are successively inoculated into the inoculation hole by breaking off the seed pieces from the grooves.