JPH0363569A - Serum separation accelerator and test tube for serum separation - Google Patents

Abstract

PURPOSE:To provide the serum sepn. accelerator which is inexpensive, is easy to handle and does not affect an analysis value by using <=40nun fine powder of the acid resistant glass. CONSTITUTION:The fine powder of the acid resistant glass (C glass) sized <=40mum is charged into a plastic test tube and is used as the serum sepn. accelerator. Since the acid resistant glass is used, there are no eluting components from the glass into blood. The fine powder of the acid resistant glass sized preferably <=15mum exists preferably at >= 90% in the fine powder. The preferable amt. of the acid resistant glass to be used is 0.04 to 50mg per 1ml blood.

Description

DETAILED DESCRIPTION OF THE INVENTION [Field of Industrial Application] The present invention relates to a serum separation promoter and a serum separation test tube equipped with the same.

[Conventional technology]

In a blood test, collected blood is centrifuged, serum components are separated and extracted, and the components are analyzed. The test tubes used to collect blood for this purpose are changing from glass to plastic. This is because glass is expensive and has a high possibility of being damaged during handling. However, when plastic test tubes are used, fibrin monomers are mixed into the serum phase during serum separation, which often causes coagulation in the serum phase after separation. Coagulation in the serum phase interferes with subsequent tests, resulting in blood sampling having to be repeated.

Therefore, it is recommended to use various serum separation promoters and sealants that make use of the difference in specific gravity to form an intermediate phase between the serum phase and the blood cell phase during centrifugation.
No. 9 and Japanese Unexamined Patent Publication No. 195452/1983.

[Problem to be solved by the invention]

However, in conventionally known examples, fibrous or powdered glass is cited as particularly effective. The size of fibrous glass is mentioned, but powdered glass is not mentioned. Also, the composition of these glasses is not described.

In addition, in recent years, there has been an increase in the number of cases in which tests on blood collected at hospitals are outsourced to specialized external institutions.

At this time, it is necessary to cool the blood between collection and analysis to prevent deterioration. However, blood and serum separated from blood that has been cooled for more than several tens of minutes has a higher probability of coagulating than blood that has been kept at room temperature, and conventional serum separation promoters are not effective enough. There is.

The purpose of this invention is to use a glass powder that is inexpensive, easy to handle, and does not affect analytical values as a serum separation promoter to prevent coagulation after serum is separated from blood that has been under cooling. To obtain an unused serum separation promoter and a test tube for serum separation using the same.

[Means to solve the problem]

In the present invention, acid-resistant glass fine powder with a size of 40 μm or less is put into a plastic test tube and used as a serum separation promoter. Acid-resistant glass is also called C glass, and its composition is as follows.

5iOz: 50-70%, BzOi: 2-10
%.

MgO: 0-5%, A]z03+Faz○3: 2-
16%C30:4~15%+N*zO+K
z○: 3-15%.

Zゎ○: 0-10%.

If there are components eluted from the glass into the blood, it may affect the analytical values, and it is not always possible to make a diagnosis that something is wrong with your health when abnormal values appear as a result of a blood test. Therefore, in the present invention, the material is limited to acid-resistant glass.

Elution components depending on the type of glass were investigated in the following manner for acid-resistant glass and alkali-free glass (referred to as E-glass, which is mass-produced) according to the present invention. Even when it is actually used for blood tests, it is difficult to clearly distinguish the superiority of the results due to the type of glass. Therefore, 1 g of fine powder of both glasses was boiled in 30 d of 0.01N hydrochloric acid for 1 minute, and after cooling for 1 hour, various cation analyzes were conducted on the supernatant liquid. The results are shown in Table 1 below.

Elution of F62゛C, Pa was observed from the alkali-free glass fine powder, but this was not the case with the acid-resistant glass fine powder.

Furthermore, in order to prevent elution of other glass components, this acid-resistant glass fine powder may be purified by boiling it in dilute hydrochloric acid or the like in advance.

The particle size of the acid-resistant glass fine powder of the present invention is 40 μm
It is as follows. Preferably, 90% or more of the particles are 15 μm or less. This was observed and measured using a microscope. The effectiveness of glass powder with small particles was investigated as follows.

Place 15 1n diameter glass beads in a plastic test tube.
The one with 0■ addition, the one with 450■ addition, and the 40 of the present invention
Four types were compared: one to which 5 Oflv of acid-resistant glass fine powder of µm or less was added, and one to which no additive was added as a control. The operation was performed at room temperature. First, put 5 in a plastic test tube.
wrll's blood was added and centrifuged after 10 minutes. The mixture was left as it was for 30 minutes, and when coagulation started for the serum, each additive was added, shaken, and left for another 30 minutes.

Then, the mixture was centrifuged again and the serum content was observed over time.

The results are shown in Table 2 below. Turbidity or coagulation occurred in the case with no additives and the case with 150 μg of glass peas. The solution containing 450 μg of glass peas was clear after 7 minutes, but became cloudy after 15 minutes. The one to which 50 mg of acid-resistant glass fine powder was added remained clear even after 30 minutes.

Therefore, it can be seen that the smaller the glass particles, the greater the effect with a small amount. This fine glass powder is obtained by finely pulverizing acid-resistant glass and passing it through a 325 mesh sieve, but commercially available products can also be used. Since this particle has an amorphous shape and is not spherical, a particle that passes this point has a diameter of 40 μm.
No samples exceeding m were observed. Therefore, the particle size was set to 40 μm or less as a small particle size that could be obtained without increasing costs.

The preferred amount of acid-resistant glass fine powder to be used is 0.04 to 50 mg per blood l-. If the amount is more than 50 ■, it is effective, but there is a risk of causing hemolysis that destroys blood cells. Further, the lower limit value is based on the following experimental results.

5.0cm of acid-resistant glass fine powder with a size of 40μm or less
, 0.5■, 0.2■, 0.1■, and omg were placed in plastic test tubes, respectively. Five portions of each sample of blood were added thereto, stored in a refrigerator at 5°C for 4 hours, centrifuged, and the serum phase obtained was observed. Blood from 20 people was used.

As a result, 0.2■ for blood 5-, that is, blood 1
When 0.04 μm or more per ml was used, a clear serum phase was obtained, but when it was less than this, turbidity and coagulation occurred.

Further, in the present invention, for example, 2
00■ is dispersed in a liquid 100ynR that does not affect blood analysis measurements such as purified water, prepared in a suitable container such as a bottle, and shaken before use to make it uniformly dispersed. Using a quantitative pipette, pipet a fixed amount into a plastic blood collection tube. The liquid is then evaporated by drying under reduced pressure, and the tube is used as a test tube for blood collection.

A suitable stopper is used for this test tube because the fine glass powder inside is not fixed within the test tube. Even if the fine glass powder scatters in the test tube and adheres to the vessel wall or the back of the stopper after the stopper is put on and before use, the fine glass powder will be removed at that time because it is shaken after the blood is added. It comes into contact with blood and the entire amount can be used effectively.

Table 1 Table 2 Changes in hemolysis serum phase: No hemolysis ○: Clear and smooth △: Slightly cloudy Blood is added to the powder. The test tube is stoppered, shaken, and refrigerated at 5°C. In this process, the surface of the pulverized glass comes into sufficient contact with the blood, and fibrin monomers in the blood are converted to fibrin, promoting the coagulation effect of the blood. Therefore, the serum obtained by subsequent separation using a centrifuge does not contain fibrin monomers and does not coagulate.

〔Effect of the invention〕

Blood to be analyzed is placed in a plastic test tube equipped with the acid-resistant glass fine powder, shaken, cooled for several tens of minutes, and then centrifuged. Blood cells and fibrin are precipitated, and the serum phase is supernatant. obtained as.

Since the serum obtained in this way does not coagulate, blood analysis operations can be performed without any problems.

Furthermore, since fine powder of acid-resistant glass is used, components eluted from the glass do not affect the measured values.

A dispersion of fine glass powder allows a certain amount of fine glass powder to be accurately and easily put into a test tube. A test tube prepared by putting this fine glass powder dispersion into a test tube and then drying the liquid is highly convenient because blood can be immediately added to the test tube and subsequent operations can be carried out.

[Example 1] Acid-resistant glass was crushed and passed through a 325 mesh sieve to obtain a fine powder of 40 μm or less. Disperse 1 g of this fine powder in 500 g of purified water, and
I put 2.51nl into each book. This was dried under reduced pressure to prepare a plastic test tube containing 5 mg of acid-resistant glass fine powder. Two test tubes were used for each person, and five tubes of blood from 60 people were put into each tube. Of these, 60 participants were used for condition A, and the remaining half were used for condition B. Condition A was to leave the blood at room temperature for 30 minutes after adding it, and then to store it in the refrigerator for 2 hours. Condition B was to add blood and immediately store it in the refrigerator for two and a half hours.

The whole was then centrifuged and the separated serum phase was observed.

[Example 2] The same procedure as Example 1 was carried out except that a 625 mesh sieve was used to obtain a fine powder of 20 μm or less.

[Example 3] The same procedure as Example 1 was carried out except that the amount of acid-resistant glass fine powder put into the test tube was changed to 2.5 μm.

[Comparative Example 1] The same procedure as Example 1 was carried out except that a 150-mesh sieve was used to obtain a fine powder of 100 μm or less.

[Comparative Example 2] Example 1 except that acid-resistant glass fine powder was not used.
I made it the same as

[Comparative Example 3] Acid-resistant glass! Same as Example I except that instead of using a powder, one drop of a commercially available serum separation enhancer was used.

The results of each of the above Examples and Comparative Examples were evaluated based on the percentage of 60 samples in which the separated serum phase remained clear, and are shown in Table 3 below.

When acid-resistant glass fine powders of 40 μm or less and 20 μm or less were used, the serum phase was 100% clear in both cases A and B.

Table 3

Claims (2)

[Claims] (1) A serum separation promoter that is put into a plastic test tube and is made of acid-resistant glass fine powder with a size of 40 μm or less. (2) A test tube for serum separation, containing the serum separation promoter according to claim 1 at a ratio of 0.04 to 50 mg per ml of blood.