JPH0369068B2 - - Google Patents
Info
- Publication number
- JPH0369068B2 JPH0369068B2 JP58236230A JP23623083A JPH0369068B2 JP H0369068 B2 JPH0369068 B2 JP H0369068B2 JP 58236230 A JP58236230 A JP 58236230A JP 23623083 A JP23623083 A JP 23623083A JP H0369068 B2 JPH0369068 B2 JP H0369068B2
- Authority
- JP
- Japan
- Prior art keywords
- adsorption column
- liquid
- switching valve
- container
- pump
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/483—Physical analysis of biological material
- G01N33/487—Physical analysis of biological material of liquid biological material
Landscapes
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Physics & Mathematics (AREA)
- Food Science & Technology (AREA)
- Biochemistry (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Biophysics (AREA)
- Medicinal Chemistry (AREA)
- Analytical Chemistry (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
- Extraction Or Liquid Replacement (AREA)
Description
【発明の詳細な説明】
本発明は血液、尿等中から薬物を抽出する方法
に関するものであり、更に詳細には従来の液−液
抽出法に代わる固−液抽出法を採用して、血液、
尿等中からの薬物の抽出を自動的に行うことがで
きるようにしたものである。DETAILED DESCRIPTION OF THE INVENTION The present invention relates to a method for extracting drugs from blood, urine, etc. More specifically, the present invention relates to a method for extracting drugs from blood, urine, etc. ,
This system is designed to automatically extract drugs from urine, etc.
従来、血液や尿等中から薬物、例えば覚醒剤、
催眠剤、精神安定剤、大麻成分、麻薬、ステロイ
ド、濃薬、カテコールアミン、プロスタグランジ
ン等を抽出する方法としては、有機溶剤を用いる
液−液抽出法が用いられているが、抽出操作中に
おける乳濁化や境界層の分離不充分などにより、
抽出効率がきわめて悪いと共に、抽出時間も長び
き、その上抽出操作に手間取る等のマイナス点が
多く、抽出、分析の迅速化、簡便化、効率化等が
望まれているにも拘らず、この要求に沿い難いも
のであつた。 Conventionally, drugs such as stimulants,
Liquid-liquid extraction using organic solvents is used to extract hypnotics, tranquilizers, cannabis components, narcotics, steroids, concentrated drugs, catecholamines, prostaglandins, etc. Due to emulsification and insufficient separation of the boundary layer, etc.
There are many negative points such as extremely low extraction efficiency, long extraction time, and time-consuming extraction operations.Despite the desire for faster, simpler, and more efficient extraction and analysis, It was difficult to meet the requirements.
本発明は斯かる点に鑑みなされたものであつ
て、従来の液−液抽出法に代わる固−液抽出法を
採用し、水溶液中に溶けている疏水性薬物をPHの
調節により難溶状態としてオクタデシルシリカ
（ODS）を主成分とする吸着剤中に通し、吸着、
濃縮を行つた後、水溶性物質を洗浄除去後、メタ
ノール等の可溶性溶剤により溶出することにより
薬物を抽出するようになしたものであり、自動的
にしかも定量的に抽出を行うことができ、抽出、
分析の迅速化、簡便化、効率化等の要求に沿うべ
くなされたものである。 The present invention has been developed in view of the above, and employs a solid-liquid extraction method instead of the conventional liquid-liquid extraction method, and allows hydrophobic drugs dissolved in an aqueous solution to be brought to a poorly soluble state by adjusting the pH. Pass it through an adsorbent whose main component is octadecyl silica (ODS) to adsorb it,
After concentration, the drug is extracted by washing and removing water-soluble substances and eluting it with a soluble solvent such as methanol, and the extraction can be carried out automatically and quantitatively. extraction,
This was done to meet the demands for faster, simpler, and more efficient analysis.
以下、図面を参照しつつ本発明について更に詳
細に説明する。 Hereinafter, the present invention will be explained in more detail with reference to the drawings.
図面は本発明に係る血液、尿等中の薬物自動抽
出方法に使用する装置の構成図である。 The drawing is a configuration diagram of an apparatus used in the method for automatically extracting drugs from blood, urine, etc. according to the present invention.
ターンテーブル（図示せず）に保持した血液、
尿等の試料液１を入れた試料液容器２と前記ター
ンテーブル外に固定した水、希アルコール水、希
アセトン水等からなる洗浄液３を入れた洗浄液容
器４との間において交互に吸引すべく移動、昇降
可能に保持された注入針５を、途中部にポンプ６
及び切換バルブ７を設けたパイプ８をもつて内部
にオクタデシルシリカ（ODS）を主成分とする
吸着剤を充填した吸着カラム９に接続し、該吸着
カラム９をパイプ１０をもつてターンテーブル
（図示せず）に保持した抽出液容器１１と前記タ
ーンテーブル外に固定したドレン受１２との間に
おいて交互に吐出すべく移動可能に保持された溶
出針１３に接続し、更に酸性メタノール等からな
る溶出液１４を入れた溶出液容器１５と酸性アセ
トニトリル等の再生液１６を入れた再生液容器１
７を、途中部に切換バルブ１８及びポンプ１９を
設けたパイプ２０をもつて前記パイプ８の切換バ
ルブ７に接続して前記吸着カラム９に接続し、試
料液容器２と吸着カラム９間のポンプ６をもつて
所要量の試料液１を吸着カラム９に送液して吸着
剤に薬物を吸着させ、その後注入針５を洗浄液容
器４に移動させて所要量の洗浄液３を吸着カラム
９に送液した後該注入針５を上昇させて所要時間
空気を吸入してパイプ８，１０及び吸着カラム９
中の液をドレン受１２中に吐出させ、更に溶出針
１３を抽出液容器１１上に移動させた後切換バル
ブ７を切換え、ポンプ１９をもつて溶出液１４を
吸着カラム９に送液して吸着カラム９中に吸着し
た薬物を溶出して抽出液容器１１に吐出させ、そ
の後溶出針１３をドレン受１２上に移動させた後
切換バルブ１８を切換え、ポンプ１９をもつて再
生液１６を吸着カラム９に送液して吸着カラム９
の再生を行い、その後更に注入針５を下降させて
切換バルブ７を切換え、ポンプ６をもつて洗浄液
３を所要量送液し、全流路を洗浄液３に置換した
後ターンテーブルを回転させ、再び新たな試料液
を吸引し、もつて血液、尿等の試料中からの薬物
の抽出を順次自動的に行うようにしたことを特徴
とするものである。 blood held on a turntable (not shown);
In order to alternately draw suction between a sample liquid container 2 containing a sample liquid 1 such as urine and a washing liquid container 4 containing a washing liquid 3 consisting of water, dilute alcohol water, dilute acetone water, etc. fixed outside the turntable. An injection needle 5, which is held movable and movable up and down, is placed in the middle with a pump 6.
A pipe 8 equipped with a switching valve 7 is connected to an adsorption column 9 filled with an adsorbent whose main component is octadecyl silica (ODS), and the adsorption column 9 is connected to a turntable (Fig. The elution needle 13 is connected to an elution needle 13 which is movably held in order to discharge the liquid alternately between an extraction liquid container 11 held in a container (not shown) and a drain receiver 12 fixed outside the turntable, and an elution needle 13 made of acidic methanol, etc. An eluate container 15 containing a liquid 14 and a regenerating liquid container 1 containing a regenerating liquid 16 such as acidic acetonitrile.
7 is connected to the switching valve 7 of the pipe 8 with a pipe 20 provided with a switching valve 18 and a pump 19 in the middle, and connected to the adsorption column 9, and a pump between the sample liquid container 2 and the adsorption column 9 is connected to the switching valve 7 of the pipe 8. 6 to send the required amount of sample solution 1 to the adsorption column 9 so that the drug is adsorbed to the adsorbent, and then move the injection needle 5 to the washing liquid container 4 to send the required amount of washing liquid 3 to the adsorption column 9. After the liquid has been drained, the injection needle 5 is raised and air is sucked for a required period of time, and the pipes 8, 10 and adsorption column 9 are
After the liquid inside is discharged into the drain receiver 12 and the elution needle 13 is moved above the extract container 11, the switching valve 7 is switched and the eluate 14 is sent to the adsorption column 9 using the pump 19. The drug adsorbed in the adsorption column 9 is eluted and discharged into the extraction liquid container 11. After that, the elution needle 13 is moved onto the drain receiver 12, the switching valve 18 is switched, and the regeneration liquid 16 is adsorbed using the pump 19. Adsorption column 9 by sending liquid to column 9
After that, the injection needle 5 is further lowered to switch the switching valve 7, and the pump 6 is used to send the required amount of cleaning liquid 3. After replacing the entire flow path with the cleaning liquid 3, the turntable is rotated. This device is characterized in that a new sample liquid is aspirated again, and then drugs are automatically extracted from samples such as blood and urine in sequence.
尚、前記吸着カラム９は通常は内径４〜12mm、
長さ20〜40mmのチユーブにオクタデシルシリカ
（ODS）を主成分とする吸着剤を充填したものを
使用する。また、洗浄液３は注入針からの汚染の
危険性を考慮して１回抽出毎に全部新しい洗浄液
に交換補充するようになつている。 Note that the adsorption column 9 usually has an inner diameter of 4 to 12 mm,
A tube with a length of 20 to 40 mm filled with an adsorbent whose main component is octadecyl silica (ODS) is used. Further, in consideration of the risk of contamination from the injection needle, the cleaning liquid 3 is completely replaced and replenished with a new cleaning liquid every time extraction is performed.
本発明は上記の如く、従来の液−液抽出法に代
わる固−液抽出法を採用し、薬物の吸着−洗浄−
溶出−再生−洗浄液置換を自動的に行うことによ
り、血液や尿試料中の薬物を自動的にしかも定量
的に抽出することができ、且つ薬物の抽出、分析
上における迅速化、簡便化、効率化、衛生的等の
要望にも充分応えることができるものである。し
たがつて、従来方法による場合には実験者１人で
10時間以上も費やす作業が、本発明方法では無人
でしかも３〜５時間で行うことができるものであ
り、きわめて効率の良い抽出法である。 As described above, the present invention employs a solid-liquid extraction method in place of the conventional liquid-liquid extraction method, and uses drug adsorption, washing, and
By automatically performing elution, regeneration, and washing solution replacement, drugs in blood and urine samples can be extracted automatically and quantitatively, and drug extraction and analysis are faster, simpler, and more efficient. It can also fully meet the demands for cleanliness, hygiene, etc. Therefore, when using the conventional method, only one experimenter can
The process of the present invention, which would otherwise take more than 10 hours, can be carried out unattended and in 3 to 5 hours, making it an extremely efficient extraction method.
図面は本発明に係る血液、尿等中の薬物自動抽
出方法に使用する装置の構成図である。
１…試料液、２…試料液容器、３…洗浄液、４
…洗浄液容器、５…注入針、６…ポンプ、７…切
換バルブ、８…パイプ、９…吸着カラム、１０…
パイプ、１１…抽出液容器、１２…ドレン受、１
３…溶出針、１４…溶出液、１５…溶出液容器、
１６…再生液、１７…再生液容器、１８…切換バ
ルブ、１９…ポンプ、２０…パイプ。
The drawing is a configuration diagram of an apparatus used in the method for automatically extracting drugs from blood, urine, etc. according to the present invention. 1...Sample liquid, 2...Sample liquid container, 3...Washing liquid, 4
...Washing liquid container, 5...Injection needle, 6...Pump, 7...Switching valve, 8...Pipe, 9...Adsorption column, 10...
Pipe, 11... Extract container, 12... Drain receiver, 1
3...Elution needle, 14...Eluate, 15...Eluate container,
16...Regeneration liquid, 17...Regeneration liquid container, 18...Switching valve, 19...Pump, 20...Pipe.
Claims (1)
液１を入れた試料液容器２と前記ターンテーブル
外に固定した水、希アルコール水、希アセトン水
等からなる洗浄液３を入れた洗浄液容器４との間
において交互に吸引すべく移動、昇降可能に保持
された注入針５を、途中部にポンプ６及び切換バ
ルブ７を設けたパイプ８をもつて内部にオクタデ
シルシリカを主成分とする吸着剤を充填した内径
４〜12mm、長さ20〜40mmの吸着カラム９に接続
し、該吸着カラム９をパイプ１０をもつてターン
テーブルに保持した抽出液容器１１と前記ターン
テーブル外に固定したドレン受け１２との間にお
いて交互に吐出すべく移動可能に保持された溶出
針１３に接続し、更に酸性メタノール等からなる
溶出液１４を入れた溶出液容器１５と酸性アセト
ニトリル等の再生液１６を入れた再生液容器１７
を途中部に切換バルブ１８及びポンプ１９を設け
たパイプ２０を前記パイプ８の切換バルブ７に接
続して前記吸着カラム９に接続し、試料液容器２
と吸着カラム９間のポンプ６をもつて所要量の試
料液１を吸着カラム９に送液して吸着剤に薬物を
吸着させ、その後注入針５を洗浄液容器４に移動
させて所要量の洗浄液３を吸着カラム９に送液し
た後該注入針５を上昇させて所要時間空気を吸入
してパイプ８，１０及び吸着カラム９中の液をド
レン受１２中に吐出させ、更に溶出針１３を抽出
液容器１１上に移動させた後切換バルブ７を切換
え、ポンプ１９をもつて溶出液１４を吸着カラム
９に送液して吸着カラム９中に吸着した薬物を溶
出して抽出液容器１１に吐出させ、その後溶出針
１３をドレン受１２上に移動させた後切換バルブ
１８を切換え、ポンプ１９をもつて再生液１６を
吸着カラム９に送液して吸着カラム９の再生を行
い、その後更に注入針５を下降させて切換バルブ
７を切換え、ポンプ６をもつて洗浄液３を所要量
送液し全流路を洗浄液３に置換した後、再び新た
な試料液を吸引し、もつて血液、尿等の試料中か
らの薬物の抽出を順次自動的に行うようにしたこ
とを特徴とする血液、尿等中の薬物自動抽出方
法。1 A sample liquid container 2 containing a sample liquid 1 such as blood or urine held on a turntable, and a cleaning liquid container 4 containing a cleaning liquid 3 made of water, dilute alcohol water, dilute acetone water, etc. fixed outside the turntable. An injection needle 5, which is held movable and movable up and down for alternate suction, is connected to a pipe 8 with a pump 6 and a switching valve 7 in the middle, and an adsorbent containing octadecyl silica as a main component is inside. An extract container 11 connected to a packed adsorption column 9 having an inner diameter of 4 to 12 mm and a length of 20 to 40 mm and holding the adsorption column 9 on a turntable with a pipe 10, and a drain receiver 12 fixed outside the turntable. An eluate container 15 containing an eluate 14 such as acidic methanol and a regenerating liquid 16 containing a regenerating liquid 16 such as acidic acetonitrile is connected to an elution needle 13 which is movably held so as to discharge alternately. Liquid container 17
A pipe 20 having a switching valve 18 and a pump 19 in the middle is connected to the switching valve 7 of the pipe 8 and connected to the adsorption column 9, and the sample liquid container 2 is connected to the switching valve 7 of the pipe 8.
A required amount of sample solution 1 is sent to the adsorption column 9 using a pump 6 between the adsorption column 9 and the drug adsorbed to the adsorbent, and then the injection needle 5 is moved to the washing liquid container 4 to dispense the required amount of washing liquid. 3 to the adsorption column 9, the injection needle 5 is raised to suck in air for the required time to discharge the liquid in the pipes 8, 10 and the adsorption column 9 into the drain receiver 12, and then the elution needle 13 is After moving it onto the extract container 11, the switching valve 7 is switched, and the eluate 14 is sent to the adsorption column 9 using the pump 19, and the drug adsorbed in the adsorption column 9 is eluted and transferred to the extract container 11. After discharging, the elution needle 13 is moved onto the drain receiver 12, the switching valve 18 is switched, and the regenerating liquid 16 is sent to the adsorption column 9 using the pump 19 to regenerate the adsorption column 9. After lowering the injection needle 5 and switching the switching valve 7, pumping the required amount of cleaning liquid 3 using the pump 6 and replacing the entire flow path with the cleaning liquid 3, a new sample liquid is aspirated again, and blood, 1. A method for automatically extracting drugs from blood, urine, etc., characterized in that drugs are automatically extracted sequentially from samples such as urine.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP23623083A JPS60128367A (en) | 1983-12-16 | 1983-12-16 | Automatic extraction of medicinal substance in blood, urine or the like |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP23623083A JPS60128367A (en) | 1983-12-16 | 1983-12-16 | Automatic extraction of medicinal substance in blood, urine or the like |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS60128367A JPS60128367A (en) | 1985-07-09 |
| JPH0369068B2 true JPH0369068B2 (en) | 1991-10-30 |
Family
ID=16997717
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP23623083A Granted JPS60128367A (en) | 1983-12-16 | 1983-12-16 | Automatic extraction of medicinal substance in blood, urine or the like |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS60128367A (en) |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5338958B2 (en) * | 1973-08-20 | 1978-10-18 |
-
1983
- 1983-12-16 JP JP23623083A patent/JPS60128367A/en active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| JPS60128367A (en) | 1985-07-09 |
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